materials dna extraction and 454 life sciences sequencing

... Research) and high throughput genomic sequences, which were matched with the EST sequences and used to design specific primers (Additional file 2) for DNA and cDNA amplification in lines F2 and F252 ... down-regulation and up -and- down-regulation (Fig 3) Expression of LEA Emb5 and Rab17 (Dhn1) genes and dehydrin Rab28 gene increased during desiccation, especially in F252 at 60 and 80 DAP (Fig ... TIP2;2 on bin 5.07 and PIP1;5, PIP2;1, PIP2;4 and PIP2;6 on bin 7.02) and LEA proteins (Rab28 on bin 5.02, Rehydrin on bin 7.05 and Rab17 on bin 9.04) Transcript expression of candidate genes related...

Ngày tải lên: 12/08/2014, 03:21

... Genomic structure and expression analysis of the Cc RNase epidermis and intestine of 6-day-old larvae and adult heads and ovaries, was blotted and hybridized using the Cc RNase cDNA as a probe As ... Cc RNase cDNA The selected hybrids were amplified by PCR and cloned into the pCR 2.1 vector Twenty-four cDNA clones were selected and verified by restriction endonuclease mapping and DNA sequence ... and the extracted DNA was cloned into the pCR 2.1 cloning vector Three clones were selected, and their authenticity was verified initially by restriction endonuclease mapping and then by DNA sequencing...

Ngày tải lên: 23/03/2014, 06:20

... migration profiles of core+1 ⁄ F–myc and core+1 ⁄ S–myc proteins, at  22 and 13 kDa, respectively, are indicated by the open and filled arrowheads The arrows show core and actin The migration positions ... codons 8–11 myc myc myc – 342–825 pHPI-1494 (pcDNA3.1(–) ⁄ Myc-His) pHPI-1507 (pcDNA3.1(–) ⁄ Myc-His) pHPI-1495 (pcDNA3.1(–) ⁄ Myc-His) pHPI-1705 (pcDNA3.1(–) ⁄ Myc-His) Mutation Tag molecule Length ... 0.4 lgÆwell)1) The quantity of transfected DNA was kept constant (0.8 lg DNA well)1) by the addition of the parental plasmid pA-EUA2 The quantity of DNA used for transfection is indicated in micrograms...

Ngày tải lên: 30/03/2014, 03:20

... start to walk and refuse to rest They just want to walk but have no idea where they are walking to Some patients clap their hands on and on, others fold and refold laundry over and over They ... showed later that mean p25/p35 ratios were about 1.7 and for control and AD brains respectively (Tseng et al., 2002) One the other hand, Tandon and his colleagues pointed out that no elevation of ... dopamine receptors, and the proliferation of smooth muscle cells in blood vessel walls and so on The identification of the canonical aging changes and further research on why and how they happen...

Ngày tải lên: 05/10/2015, 22:32

... start to walk and refuse to rest They just want to walk but have no idea where they are walking to Some patients clap their hands on and on, others fold and refold laundry over and over They ... showed later that mean p25/p35 ratios were about 1.7 and for control and AD brains respectively (Tseng et al., 2002) One the other hand, Tandon and his colleagues pointed out that no elevation of ... dopamine receptors, and the proliferation of smooth muscle cells in blood vessel walls and so on The identification of the canonical aging changes and further research on why and how they happen...

Ngày tải lên: 06/10/2015, 20:35

... comprises at least two genes ZmSUN1 and ZmSUN2 are CCSD-type and are most closely related to plant SUNdomain homologs AtSUN1, AtSUN2, and OsSad1 ZmSUN3, 4, and are PM3-type and probably represent a previously ... presence and localization of ZmSUN3/4 proteins in planta, we developed peptide antibodies for western blotting and immunolocalization, and the results are summarized in Figure and The peptides used and ... LAVIFLSFVF ACLALAKLSI Figure ZmSUN4 cDNA and protein features (A) ZmSUN4 (genotype W23) full-length cDNA, showing the 5’ and 3’ UTRs, open reading frame (ORF), and poly-A tail A diagram of the protein...

Ngày tải lên: 11/08/2014, 11:21

... A260/A280 ratios between 1.9 and 2.1 and A260/A230 ratios greater than 2.0 were used for the analysis First strand cDNAs were synthesized from 1.5 g of total RNA with OligodT and Superscript II enzyme ... from ovaries of apomictic plants in megasporogenesis and megagametogenesis This library was validated by sequencing and annotating 2,000 clones, and out of these sequences, eight genes were chosen ... the same extraction protocol [14] for the different Brachiaria organs All samples were treated with DNAse to avoid misinterpretation of qRT-PCR results by genomic DNA contamination in cDNA samples...

Ngày tải lên: 12/08/2014, 03:20

... target and novel visual stimuli in children of alcoholics Alcohol 1998; 15:119-36 92 Porjesz B And Begleiter H Human brain electrophysiology and alcoholism In: Tarter R and Thiel DV, Eds Alcohol and ... inverse relation between age and callosal size Conversely, a subsequent review of MRI and post-mortem reports indicated a callossal volume relationship to handedness and gender [69]; the clinical ... both active and passive P300 latency and age; in this same study, levels of active latency were associated with concentration ability, and passive latency with verbal proficiency and recall [72]...

Ngày tải lên: 02/11/2012, 11:08

... Environment, Agricultural Sciences and Spatial Planning (to L.W and S.E.), the 4928 Swedish Research Council (to H.E and P.N.) and the Swedish Cancer Foundation (to H.E and P.N.) The Structural ... domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig 1A) The two subunits ... PRTFDC1 (A) monomer and (B) tetramer generated using symmetry-related molecules Interactions with GMP (C) GMP, phosphate and calcium ions are shown as sticks and spheres colored pink and black, respectively...

Ngày tải lên: 15/02/2014, 01:20

... rate constants are similar between NP and MP and 2248 between HP and HPO in accordance with the similar shapes and sizes between NP and MP and between HP and HPO The different Kd values are proportional ... HP and 20 mM GA The reaction was initiated with 10 lM SaDHNA at 25 °C and quenched with M HCl The reverse reaction generated both DHNP and DHMP HP, DHNP, and DHMP were oxidized to HPO, NP, and ... using both DHNP and DHMP as the substrates and the reverse reaction (the formation of DHNP and DHMP) with HP and GA For the forward reaction, three concentrations each for DHNP and DHMP were used...

Ngày tải lên: 19/02/2014, 00:20

... gradient (broken line), and concentrations of LPL (j) and lipase activities (h) were measured in each fraction as described in the Materials and methods The upper, middle, and lower panels are the ... in green, and the cluster of hydrophobic residues Trp390, Trp393, and Trp394 is in red The catalytic site is enlarged in B and illustrates the conserved disulfide bridge between Cys239 and Cys216 ... sequence containing Trp393 and Trp394 Fig Heparin binding of wild-type LPL and the WW mutant Wild-type LPL (left) and WW mutant (right) were applied to heparinSuperose FPLC and eluted by a linear...

Ngày tải lên: 21/02/2014, 15:20

... black and hog-like bestiality, and fox and wolf-like cunning and ferocity with wicked human thought and self-command, which Raphael has enshrined in that splendid harmony of scarlet silk and crimson ... Ivon and Ivaire, the Gascon Engelier, Gộrier and Gộrin, Bộrenger and Otho, Anseis and Salamon, and the old Gerard of Rousillon; and one by one drags them to where the archbishop lies dying And ... piles of cabbage and cauli-flowers, the rows of tin ware and copper saucepans, the heaps of maccaroni and pastes, of spices and drugs; the garlands of onions and red peppers and piles of apples;...

Ngày tải lên: 21/02/2014, 21:20

... spectra between free and DNA- bound RepA are shown as dashed lines RepA was 1.25 lM and 0, 0.2, 0.4, 0.6 and lM 1IR (A), 0, 0.4, 1.2, 2.4 and lM 1DR (B), and 0, 0.8, 1.8, 3.2 and lM 1DR-short (C) ... (A) and bound to 1IR (B), 1DR (C) and 1DR-short (D) Table S1 Fluorescence lifetimes and decay amplitudes for W94 and AEDANSC160, respectively, in free RepA and RepA bound to various cognate DNA ... and DNA, were removed by chromatography (MA7), and the oligonucleotides were subsequently annealed with their complementary strands, and puried as above UV Vis and CD spectroscopy CD spectra and...

Ngày tải lên: 07/03/2014, 04:20

... infected fish, a full-length cDNA clone as well as a genomic DNA clone were isolated and sequenced, and the expression and modulation of this molecule was studied Results Cloning and characterization ... the submitted sequences The 5¢- and 3¢-flanking and intron sequences are in lowercase Identical nucleotides in the cDNA to genomic DNA sequence is relaced by ‘|’ and insertions indicated by ‘-’ ... bold and italics The ATTTA motifs in the 5¢- and 3¢-UTRs, the start and stop codons, the signal peptide predicted by SIGNALP 3.0 [23] and the potential N-glycosylation site (NQT) are in bold and...

Ngày tải lên: 07/03/2014, 16:20

... disrupted promoter structure and exons I and II, exhibit impaired follicular development and ovulation [25] and impaired spermiogenesis in males [26] On the other hand, mice defective in Camk4 ... hemisphere and between cerebellum primordium and medulla oblongata (Fig 6, A1 and A2) Also border regions between metencephalon and pons with the third ventricle (arrows in Fig 6, A1 and A2) showed ... cDNA dot-blot hybridization using the human multiple tissue expression (MTE, Clontech, Palo Alto, CA) and a STK33 specific probe The cDNA dot-blot contains 76 normalized dots of immobilized cDNA,...

Ngày tải lên: 16/03/2014, 23:20

... min, and extension at 72 °C for min) and a final extension at 72 °C for 10 in a Gene Cycler (Bio-Rad, USA) The amplified DNA fragment was subcloned into a pGEM-T vector (Promega) and DNA sequencing ... single 1.6-kb cDNA clone (RK-2) was obtained and DNA sequencing showed that it shared 76% nucleotide similarity with RK-1 5¢-RACE and 3¢-RACE were performed using the Marathon cDNA amplification ... strand cDNA, · PCR buffer (20 mM Tris/HCl pH 8.4, 50 mM KCl), 0.2 lM of each primer, 1.5 mM MgCl2 and 0.2 mM of dNTPs PCR Construction and screening of a grass carp genomic DNA library Genomic DNA...

Ngày tải lên: 17/03/2014, 03:20

... tachyplesin I and polyphemusin I share two highly hydrophilic and positively charged poles in the N- and C-terminal regions and in the turn Androctonin also has a highly hydrophilic and positively ... hydrophobic residues and the disulphide bridges, and a second polar face formed by charged Arg1, Arg7 and Arg9 side-chains (see Figs and 4) In general, BM-1 was more flexible than PG-1 and IB-367, but, ... plated b-sheet structure Androctonin has an unequal number of residues on each strand between the two bridges – five in the N-terminal strand and three in the C-terminal strand This causes a greater...

Ngày tải lên: 22/03/2014, 21:20

... tryptic soy agar (TSA) and in vivo and have demonstrated that their structures are chemically and antigenically distinct from the previously described O-chain polysaccharide [11] and capsule [13] Experimental ... phenol solution (4 h, 22 °C) and harvested by centrifugation (yield 25 g, wet weight) Isolation and purification of CPS and LPS The cells were washed with 2.5% saline (w/v) and extracted by the method ... Sephadex G-100 and lyophilized (yield mg) NMR spectroscopy NMR spectra were performed on Varian INOVA 500 and 600 MHz spectrometers using standard software NMR measurements were made at 25 °C and 60...

Ngày tải lên: 23/03/2014, 13:20

... Integrated DNA Technologies (Coralville, IA, USA) Vent DNA polymerase, nucleotide triphosphates, and restriction endonucleases were purchased from New England Biolabs (Beverly, MA, USA) T4 DNA ligase ... requirement for ATP, CoA, and NADPH for the activity of FAE1 KCS FAE1 KCS showed high activity with monounsaturated and saturated C16 and C18 and no activity with polyunsaturated C18:2 and C18:3 In addition, ... DNA ligase was from Gibco BRL (Grand Island, NY, USA) Ni2+-PDC was purchased from Affiland (Affinity Methodology in Biotechnology, Belgium) Thrombin, acyl-CoAs, and galactose were purchased from...

Ngày tải lên: 24/03/2014, 03:21

... most extended 5¢-RACE products (CardA_cDNA, CardB_cDNA, and CardD_cDNA) against the corresponding genomic sequences (CardA_gDNA, CardB_gDNA, and CardC_gDNA) revealed the presence of an intron ... of the plant life cycle [16–21] In this study, the genomic sequences of the cardosin A and B genes and of two new cardosin genes (those encoding cardosins C and D) were isolated and characterized ... alignment and homology of cardosins A, B, C and D and cyprosin A and B (A) The amino acid sequences were deduced from the genomic sequences (this work), with the exception of cyprosin A (X69193) and...

Ngày tải lên: 30/03/2014, 09:20