manuals mathematics teaching methods
Safe carrying methods
Ngày tải lên :
21/08/2012, 17:56
... section 2 they will be in breach of the law and liable for a
fine of $25 000.
Safe carrying methods
Carrying heavy objects can put a strain on your back, arms, shoulders and knees
because ... that are too heavy for you to lift or carry. In these cases,
you may need to use some of these methods:
• Work with someone to carry the load.
• Use a trolley.
• Use a forklift or trolley jack...
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MOB Subject 5 –Access methods Static and dynamic access techniques
Ngày tải lên :
17/09/2012, 09:13
... - MOB Mobile Internet and Surrounding
1/12 Baey, Fladenmuller – Subject 5
MOB Subject 5 –Access methods
Static and dynamic access techniques
1. Static access techniques
1.1 Space-division multiple-access...
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Glycoprotein methods protocols - biotechnology 048-9-001-013.pdf
Ngày tải lên :
23/09/2012, 19:25
... Biochem. Mol.
Biol. 27, 5792.
Methods in Molecular Biology
TM
HUMANA PRESS
Glycoprotein
Methods
and Protocols
Edited by
Anthony P. Corfield
VOLUME 125
The Mucins
Methods in Molecular Biology
TM
HUMANA ... separation between mucins
Isolation of Large Gel-Forming Mucins 3
3
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press ... analyses.
3.6. Analysis of Mucins
Methods for the detection and analysis of mucins are dealt with in other chapters in
this volume. However, three principally different methods are available: solution
assays...
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Glycoprotein methods protocols - biotechnology 048-9-027-044.pdf
Ngày tải lên :
23/09/2012, 19:25
... nonsulfated mucins remain
unstained.
Histological Methods for Detection of Mucin 29
29
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. ... tract.
Histological Methods for Detection of Mucin 39
3. Alternatively, prepared adhesive slides such as Superfrost Plus
®
/(Menzel-Gläser,
Braunschweig, Germany) may be purchased.
4. There are many other methods ... the demonstration of mucin was mucicarmine
(4), but this stain has now been largely supplanted by methods based on more strictly
histochemical approaches that utilize a specific chemical reaction...
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Glycoprotein methods protocols - biotechnology 048-9-045-055.pdf
Ngày tải lên :
23/09/2012, 19:25
... Mucins can be detected by chemical methods within acrylamide
Detection and Quantitation of Mucins 45
45
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited ... mucins, in Glycoprotein
Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ.
3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and
Protocols: ... (6).
References
1. Walsh, M. D., Jass, J. R. (2000) Histologically-based methods for detection of mucin,
in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana,
Totowa, NJ.
2....
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Glycoprotein methods protocols - biotechnology 048-9-057-064.pdf
Ngày tải lên :
23/09/2012, 19:25
... wrapping them in a 1–cm
2
sand-
GI Adherent Mucous Gel Barrier 57
57
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press ... mucous
layer existed over the gastric mucosal surface (13,14). However, a variety of different
methods (discussed subsequently) have now demonstrated unequivocally that a thick
(100–200 µm), ... consisting primarily of a fibrin gel and necrotic cells with some mucin stain-
ing (15).
Original methods for measuring adherent mucous thickness in situ were based on
(1) differences in refractive...
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Glycoprotein methods protocols - biotechnology 048-9-065-073.pdf
Ngày tải lên :
23/09/2012, 19:25
... PhosphorImager as au/A
260nm
.
Biosynthesis and Secretion of Mucin 65
65
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press ... is isolated
according to the manufacturer. Measure the following components:
68 Dekker et al.
3. Methods
3.1. General Assays for Quantitation of Mucin (
see
Notes 1–3)
Each of these six detailed...
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Glycoprotein methods protocols - biotechnology 048-9-075-085.pdf
Ngày tải lên :
23/09/2012, 19:26
... there are few separation techniques available to use with these
77
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press ... Biotechnol. 5, 171–176.
10. Thornton, D. J., Howard, M., Devine, P. L. and Sheehan, J. K. (1995) Methods for separa-
tion and deglycosylation of mucin subunits. Anal. Biochem. 227, 162–167.
Separation ... monomers
generated after reduction (M
r
= 2–3 × 10
6
) are amenable to conventional biochemical
separation methods. We describe a separation protocol based on anion exchange chro-
matography to fractionate...
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Glycoprotein methods protocols - biotechnology 048-9-087-096.pdf
Ngày tải lên :
23/09/2012, 19:26
... 5).
Heterogeneity and Size Distribution of Gel-Forming Mucins 87
87
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press ... chapter describes only methods for assessing the heterogeneity of mucin
populations with regard to mass and size utilizing rate zonal centrifugation and electron
microscopy. Other methods for the absolute ... 96 Sheehan and Thornton
12. Koller, T., Harford, A. G., Lee, Y. K., and Beer, M. (1969) New methods for the prepara-
tion of nucleic acid molecules for electron microscopy. Micron. 1, 110–118.
13....
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Glycoprotein methods protocols - biotechnology 048-9-111-119.pdf
Ngày tải lên :
23/09/2012, 19:26
... code as defined in Fig. 1.
Amino Acid Analysis of Mucins 113
113
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press ... Mobile phase B: 15% (v/v) methanol in water.
6. Mobile phase C: 90% (v/v) acetonitrile in water.
3. Methods
3.1. Sample Preparation and Hydrolysis
1. Add an aliquot of a solution of a sample of mucin...
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Glycoprotein methods protocols - biotechnology 048-9-121-128.pdf
Ngày tải lên :
23/09/2012, 19:26
... Sequencing Glycopeptides 121
121
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press ... Elsewhere we have published alternative HPLC methods for the
separation of PTH-glycosylated amino acids on a modified Beckman LF3600
sequenator (12). These methods were developed for two reasons: ... with
high molecular weight, heavily glycosylated domains such as those found in mucins.
The two methods for determining the sites of glycosylation in proteins are mass
spectrometry and Edman sequencing....
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Glycoprotein methods protocols - biotechnology 048-9-129-141.pdf
Ngày tải lên :
23/09/2012, 19:26
... R
Trityl C
Synthetic Peptides for Antimucin Antibodies 129
129
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press ... cannot be controlled or simply that short peptides do not adhere
well to polystyrene plates. Several methods of peptide modification have been utilized
to overcome these problems. One such procedure ... epitopes defined by antibodies reactive
with antigens of known primary structure. Unlike most other methods of simultaneous
peptide synthesis, this technique allows the concurrent synthesis of hundreds...
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- glycoprotein methods protocols - biotechnology
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