maintenance of hematopoiesis role of early b cell factor 2

... antigen-experienced B cells in the aged spleen Both cytokines are known to enhance release of < /b> B cell activating factor (BAFF; also known as BLyS, TALL-1, zTNF4, and THANK) by monocytes [ 62] BAFF is a member of < /b> ... frequencies of < /b> clonable pre B cells during life in different lymphoid organs of < /b> mice Blood 1993, 81 :22 90 -23 00 Zharhary D: Age-related changes in the capability of < /b> the bone marrow to generate B cells ... 20 03, 171 :23 26 -23 30 22 Stephan RP, Lill-Elghanian DA, Witte PL: Development of < /b> B cells in aged mice: decline in the ability of < /b> pro -B cells to respond to IL-7 but not to other growth factors J...

Ngày tải lên: 09/08/2014, 01:23

... numbers of < /b> mice of < /b> each strain evaluated were as follows: (B1 0.Q × DBA/1)F1, n = 10; B1 0-Tg(DR4), n = 8; B1 0.P, n = 12; B1 0.Q, n = 26 ; B1 0.RIII, n = 6; C3H.NB, n = 9; and DBA/1, n = 10 (c) Because ... 115-035-0 62; Jackson ImmunoResearch, West Grove, PA, USA) secondary antibodies [25 ] ABTS (2, 2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid), # 1 120 4 521 001; Roche Diagnostics GmbH, Penzberg, Germany) ... haplotype H-2p on the black background rendered mice resistant to G6PI-induced arthritis, the role < /b> of < /b> the beta chain of < /b> Ap was addressed on the highly susceptible C3H background C3H.NB (H-2p; n = 21 )...

Ngày tải lên: 09/08/2014, 07:20

... an anti-CD20 monoclonal antibody (rituximab, IDEC-C 2B8 ) Blood 1999, 94(7) :22 17 -22 24 Gozzetti A, Crupi R, Tozzuoli D, Raspadori D, Forconi F, Lauria F: Molecular cytogenetic analysis of < /b> B- CLL patients ... with alemtuzumab was considered, but preference was given to rituximab because of < /b> the less severe compromise of < /b> the cellular immune system by rituximab, and because of < /b> the possibility that autoimmune ... Journal of < /b> Medical Case Reports 20 08, 2: 275 of < /b> rituximab in CLL was considered unsafe because of < /b> severe toxic reactions from tumor cell lysis in patients with very elevated blood cell counts [2] Here...

Ngày tải lên: 11/08/2014, 21:22

... targeted cells could be observed with the Lefty2 knockdown cells Consistent results were obtained using both shRNAs (Figure 22 a and 2 2b) To show that upregulation of < /b> SMAD2 phosphorylation is not an off ... phosphorylation of < /b> SMAD2 and SMAD3 by ALK4/5/7 A plausible explanation for the rescue effect of < /b> SB4315 42 is that SB4315 42 supplementation restored and brought back down the level of < /b> SMAD2/3 phosphorylation ... RNAi3+1uM SB RNAi3+DMSO Non silsil+DMSO 140 120 100 80 Nanog Lefty2 60 40 20 RNAi3+SB4315 42 RNAi3+DMSO Control+DMSO Figure 24 Effect of < /b> Lefty2 RNAi can be rescued by pharmalogical inhibition with SB431542...

Ngày tải lên: 11/09/2015, 16:02

... in both the maintenance < /b> of < /b> ES cell self renewal and induction of < /b> ES cell differentiation have both previously been reported A key objective of < /b> this thesis is to reconcile the differences between ... as Lefty2 RNAi perturbed both Oct4 and Nanog promoter activity Athough Lefty2 has previously been implicated in embryonic development and stem cell- specific expression of < /b> Lefty has been observed ... understanding of < /b> ES cell self renewal mechanism beyond the established roles of < /b> LIF/STAT3, BMP and WNT pathways and that of < /b> the core transcriptional regulatory circuitry governed by OCT4, SOX2 and NANOG...

Ngày tải lên: 11/09/2015, 16:02

... Cruz 19) Biotechnology PCNA Santa Cruz Biotechnology CATALOGUE NUMBER 566415 ab5487-50 51-1300 DILUTION RAISED IN 1/500 1/500 1/1000 Rabbit Rabbit Rabbit 9514S 1/1000 Rabbit SC- 620 0 1 /20 0 Goat ... P-Cadherin AB1 (Clone56C1) COMPANY CATALOGUE NUMBER Chemicon ab5731 Abcam ab15580 Santa Cruz SC-1616 Biotechnology BioGenex AM3 92- 5M DILUTION RAISED IN 1/1000 1/1000 1 /25 00 Rabbit Rabbit Goat 1 /20 0 Mouse ... recombinant basic fibroblast growth factor (bFGF; Invitrogen) Conditioned medium was obtained by culturing mouse embryonic fibroblast (MEF) cells with HuES9 medium The medium was collected at 24 ...

Ngày tải lên: 11/09/2015, 16:02

... Nat Biotechnol 22 , 326 –330 Rodda, D J., Chew, J L., Lim, L H., Loh, Y H., Wang, B. , Ng, H H., and Robson, P (20 05) Transcriptional regulation of < /b> nanog by OCT4 and SOX2 J Biol Chem 28 0, 24 731 -24 737 ... the absence of < /b> feeder layers Stem Cells 23 , 489-495 Besser, D (20 04) Expression of < /b> nodal, lefty-a, and lefty -B in undifferentiated human embryonic stem cells requires activation of < /b> Smad2/3 J Biol ... and Andrews, P W (20 04) Specific knockdown of < /b> Oct4 and beta2microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells Stem Cells 22 , 659-668 Matsuda,...

Ngày tải lên: 11/09/2015, 16:02

... presence of < /b> SB4315 42, the efficiency of < /b> BGN2 human ES cells to form embryoid body was markedly reduced It was reported that the few embryoid bodies which did form in presence of < /b> SB4315 42 were of < /b> an ... undertaken by the mammalian embryo As for ES cells, the function of < /b> Oct3/4 is to specify cell fate into pluripotent cells or primitive endoderm cells by blocking trophectodermal differentiation 1 .2. 2 .2 ... expressed in both human and murine embryonic stem cells and blastocysts (Brandenberger et al., 20 04; Wei et al., 20 05) Hence, it is possible that Lefty1 and Lefty2 may represent components of < /b> the critical...

Ngày tải lên: 11/09/2015, 16:02

... 77.9 122 .3 95 .2 68.8 56.5 35.5 73.6 127 .3 141.6 109 .2 94.7 52. 7 84.8 107.3 84.0 88.3 158.6 157.6 26 2.1 21 5.8 999.5 128 5.7 3 92. 6 640.8 7 62. 2 129 1 .2 226 .1 117.7 130.5 160.8 27 4.8 107.1 440.5 122 6.9 ... 117.1 22 9.7 103 .2 205.0 24 1.9 121 .1 1 82. 5 179.4 122 .5 326 .8 339.7 143.1 134.7 20 8.9 308.3 816.9 801.3 24 2.5 997.6 756.6 315.6 5 82. 9 21 5.3 1 62. 1 25 6 .2 179.6 24 6.4 568.7 467.7 461.7 62. 1 98.8 183.0 ... 109.9 120 .3 163.6 123 .3 80.7 91.0 81.5 129 .4 68.5 22 7.7 461.6 24 9.5 128 .5 24 9.6 420 .9 90.7 104.0 40.9 12. 1 73.8 122 .8 1 92. 4 330.7 59.5 96.9 124 .0 89.6 134.4 20 1 VII) A summary table documenting...

Ngày tải lên: 11/09/2015, 16:03

... cell culture methodology 2. 4.1 Cell culture media 2. 4 .2 Purification of < /b> splenic B cells 2. 4.3 Transfection of < /b> HEK 29 3T cells 2. 4.4 Transfection of < /b> A20 B cells 2. 4.5 Stimulation of < /b> A20/ BJAB cells ... project 1 10 12 13 17 20 23 26 26 26 28 29 29 31 32 32 33 35 35 36 36 39 40 42 43 CHAPTER 2: MATERIAL AND METHODS 2. 1 2. 2 2. 3 2. 4 2. 5 2. 6 List of < /b> antibodies for Immuno-fluorescence, BCR stimulation, ... 3: THE ROLE < /b> OF < /b> HIC-5 IN B CELL RECEPTOR SIGNALING 3.1 Introduction 3 .2 Results 3 .2. 1 Yeast-two-Hybrid using B cells adaptor protein, Bam 32 as a bait 3 .2. 2 Interaction of < /b> Bam 32 with Lyn 3 .2. 3 Interaction...

Ngày tải lên: 12/09/2015, 08:16

... (20 05) Mol Cell Biol 25 , 8486 – 8495 20 Robson, J D., Davidson, D., and Veillette, A (20 04) Mol Cell Biol 24 , 23 32 23 43 21 Ng, C.-H., Xu, S., and Lam, K.-P (20 07) Blood 110, 25 9 26 6 22 Kurosaki, ... Veillette, A (20 00) Mol Cell Biol 20 , 27 43 27 54 25 Schaller, M D (20 01) Oncogene 20 , 6459 – 64 72 26 Tumbarello, D A., Brown, M C., and Turner, C E (20 02) FEBS Lett 513, 114 –118 27 Davidson, D., ... loading of < /b> whole cell lysates Inhibitory Role < /b> of < /b> Leupaxin in BCR Signaling FIGURE Tyr 72 of < /b> LPXN is important for its inhibition of < /b> IL -2 production in BCR-stimulated A20 B cells A, Tyr 72 is important...

Ngày tải lên: 12/09/2015, 08:16

... Apaf-1 APS dATP/ATP 5-Aza Bak Bax Bcl -2 BH Bid tBid BIR BL BPB CARD caspase CB ced CHAPS CTB Cyt c Dark DED DEVD-AMC DIABLO DISC DMRIE-C DMSO DNA DRs DREDD DTT E2 E3 EBV EBNA mitochondria transmemrane ... NF- B- dependent anti-apoptotic gene products, 24 A XIAP BIR1 BIR2 RING BIR3 497 CARD cIAP1 CARD cIAP2 ILP -2 604 618 23 6 ML-IAP 29 8 NBD NAIP SURVIVIN 1403 1 42 4830 BRUCE RING B E2/E3 BIR Substrate: ... of < /b> Singapore i TABLE OF < /b> CONTENTS Acknowledgements i Table of < /b> Contents ii Summery vi List of < /b> Tables vii List of < /b> Figures viii Abbreviations xi List of < /b> Publications xiv PART I INTRODUCTION 1 B CELL...

Ngày tải lên: 12/09/2015, 10:17

... SC-371 SC- 728 SC-7383 3941 Goat Goat Rabbit Rabbit Rabbit Rabbit Rabbit Mouse Rabbit 1:500 1:500 1:500 1:500 1:500 1:1000 1:500 1:500 1:500 Cell Signaling Cell Signaling 921 1 925 1 Rabbit Rabbit 1:500 ... Cruz BD Pharmingen BD Pharmingen Santa Cruz Sigma Santa Cruz BD Pharmingen Biolegend Biolegend Catalog number 01 122 D 553159 0171 5B 553111 12- 025 1-83 01602D 01035A 01502D 553691 SC -20 20 020 82D 559749 ... circulation B- 2 cells express high levels of < /b> B2 20 , IgD and CD23 and moderate levels of < /b> IgM and lack surface CD5 expression The B- 1 population can be further subdivided into B- 1a and B- 1b subpopulations...

Ngày tải lên: 14/09/2015, 09:08

... Methods 2. 1 List of < /b> antibodies 47 2. 2 List of < /b> primers 48 2. 3 Molecular cloning methodology 48 2. 3.1 2. 3 .2 2.3.3 2. 3.4 2. 3.5 2. 3.6 2. 3.7 2. 3.8 2. 3.9 2. 3.10 2. 3.11 2. 3. 12 2.3.13 2. 3.14 2. 3.15 2. 3.16 ... 63 2. 5.1 Buffers and solutions 63 iii 2. 5 .2 2.5.3 2. 5.4 2. 5.5 2. 5.6 2. 5.7 2. 5.8 2. 5.9 2. 5.10 2. 5.11 2. 6 Cloning of < /b> targeting construct Culturing of < /b> mouse embryonic fibroblasts Culturing of < /b> ES cells ... Bacterial DNA mini-prep by alkaline lysis Bacterial maxi-prep using Qiagen Maxi-prep columns 60 60 2. 4.1 2. 4 .2 2.4.3 2. 4.4 2. 4.5 2. 4.6 2. 5 Mammalian cell culture methodology 60 61 61 62 62 62...

Ngày tải lên: 14/09/2015, 10:44

... NPbfuc NPbglc NPbgal NPbxyl MUbglc NPbfuc NPbglc NPbgal NPbxyl MUbglc NPbfuc NPbglc NPbgal NPbxyl MUbglc NPbfuc NPbglc NPbgal NPbxyl MUbglc 0.59 1.05 8 .2 4.9 6.3 0.38 1 .20 9.0 4.8 2. 6 0.71 2. 6 ... p-nitrophenyl b- glucoside; NPbxyl, p-nitrophenyl b- xyloside Enzyme Substrate Km (mM) kcat (s)1) E190A NPbfuc NPbglc NPbgal NPbxyl MUbglc NPbfuc NPbglc NPbgal NPbxyl MUbglc NPbfuc NPbglc NPbgal NPbxyl MUbglc ... Inhibitor Phenylbglc Methylbglc Cellobiose Cellotriose Cellotetraose Cellopentaose Hexylbglc Heptylbglc Octylbglc Nonylbglc E194A Ki (mM) 30 ± 8.0 ± 0 .2 17 ± 2. 3 ± 0 .2 2.4 ± 0.1 2. 0 ± 0.1 32 ± 21 ± 10.3...

Ngày tải lên: 18/02/2014, 17:20

... compilation ª 20 06 FEBS 3145 Reduced expression of < /b> heme oxygenase -2 20 21 22 23 24 25 26 27 28 29 30 31 Y Zhang et al explants and immortalized trophoblast cells Am J Physiol Heart Circ Physiol 28 4, H853–H858 ... microsomes J Biol Chem 25 3, 422 4– 422 9 42 Shibahara S, Yoshida T & Kikuchi G (1978) Induction of < /b> heme oxygenase by hemin in cultured pig alveolar macrophages Arch Biochem Biophys 188, 24 3 25 0 43 Okinaga ... the role < /b> of < /b> heme degradation in the population of < /b> hemoglobin-positive cells To the best of < /b> our knowledge, there has been no report on the promoter function of < /b> the human HO -2 FEBS Journal 27 3 (20 06)...

Ngày tải lên: 19/02/2014, 06:20

... )39 42; 20 68 bp), II-L ()4613 to )26 92; 1 922 bp), II-R (+50 to +1959; 1910 bp), III-L ()1369 to +578; 1948 bp), III-R (+1079–3130; 20 52 bp), IV-L ( +27 21 to +4 524 , 1804 bp), IV-R (same as 16 kb Del-R) ... site of < /b> Ig -b gene is shown as base number one): 16 kb Del-L ()11.4 kb to )9.0 kb, SacI ⁄ MluI; 2. 4 kb), 16 kb Del-R (+6703 to +8768; 20 66 bp), I-L ()10.1 kb to )7.8 kb, BamHI ⁄ XhoI; 2. 3 kb), ... hypersensitive sites in the process of < /b> gene activation J Biol Chem 26 3, 1 925 9–1 926 2 FEBS Journal 27 2 (20 05) 422 –4 32 ª 20 04 FEBS Deletion of < /b> hypersensitive sites in chicken Ig -b locus Gross DS & Garrard...

Ngày tải lên: 19/02/2014, 16:20

... after being sprayed with primulin Control SQDG Methylated SQDGa Sugar-oxidized SQDGb PtdG 189 26 1 20 7 22 3 174 Isolated thylakoid membranes Cells a b ± ± ± ± ± 21 19 14 21 22 21 0 25 6 198 23 1 20 6 ... Ó FEBS 20 02 2354 A Minoda et al (Eur J Biochem 26 9) light (90 lEÆm )2 s)1) at 28 °C with 2% CO2 bubbling The trace elements of < /b> the 3/10HSM medium were replaced by Arnon’s A5 solution ... the mutant cells in the form of < /b> liposomes [24 ], probably in the thylakoid membrane in the region of < /b> PSII The effect was observed at over 2. 4 lM of < /b> SQDG, but not at 0.48 lM When hf -2 was cultured...

Ngày tải lên: 22/02/2014, 04:20

... [22 ] [22 ] [22 ] Unpublished data [22 ] › fl fi fi fi [21 ] [21 ] [23 ] [31] [31] fi › fi fi fi [23 ] [23 ] [ 32] Unpublished data Unpublished data Unpublished data › fi fi fi fi fi cooperatively induce apoptotic cell ... Journal 27 6 (20 09) 1418–1 428 ª 20 09 The Authors Journal compilation ª 20 09 FEBS 1 425 Fine control of < /b> pre-mature B- cell apoptosis by E2A K Toyonaga et al E2A directly activates the EBF gene [26 ] and ... to apoptosis Br J Haematol 137, 3 42 348 FEBS Journal 27 6 (20 09) 1418–1 428 ª 20 09 The Authors Journal compilation ª 20 09 FEBS 1 427 Fine control of < /b> pre-mature B- cell apoptosis by E2A K Toyonaga...

Ngày tải lên: 16/03/2014, 04:20

... hydrolases Biochemistry 44, 128 09 128 18 25 Hou Y, Vocadlo D, Withers S & Mahuran D (20 00) Role < /b> of < /b> beta Arg211 in the active site of < /b> human Active site of < /b> dispersin B 26 27 28 29 30 31 beta-hexosaminidase ... Wild-type D183N E184Q E332Q W237A W330Y Y187A Y278A R27K R27A 0.011 0 .27 5 1.375 0.055 0 .27 5 0 .27 5 0. 022 0.055 0.055 0.056 1.3 8.0 a 7 .2 9.0 1.0 5.5 2. 8 5 .2 6.9 100 0.65 a 3.6 0.6 5 .2 12. 0 9.3 5.0 3.8 ... are: DspB; PDB Code: 1yht (gray) [10]; PDB Code: 2gjx (red) [30]; PDB Code: 1hp5 (green) [17]; PDB Code: 1now (blue) [16]; PDB Code: 1qbb (yellow) [20 ] Active site of < /b> dispersin B A Role < /b> of < /b> active-site...

Ngày tải lên: 16/03/2014, 06:20