m rna 30 utr binds parathyroid cytosolic proteins and determines m rna stability in response to changes in calcium and phosphate
Posttranscriptional regulation of erythropoiesis by RNA binding proteins and microRNAs
Ngày tải lên :
10/09/2015, 09:23
... then binds to target mRNAs and leads to either translational repression or mRNA destabilization37 The first evidence that microRNAs play important roles in the blood-forming system came from the ... understanding other adult stem/progenitor cell systems The long-term self-renewing hematopoietic stem cell first divides and differentiates and generates short-term self-renewing hematopoietic stem ... of lineage priming may also underlie the ability of variations in transcription factor levels to mediate alterations in lineage choice or even reprogramming within the hematopoietic system As...
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EDUS39 600 f3 FXSQ m concealed built in
Ngày tải lên :
25/09/2013, 18:22
... 15.8 mm (Flare Connection) Drain Pipe Piping Connections in (mm) VP25 External Dia 1-1/4” / 31.8 mm Internal Dia 1” / 25.4 mm VP25 External Dia 1-1/4” / 31.8 mm Internal Dia 1” / 25.4 mm VP25 ... WIRINGS • Lower the electric parts box, as shown in the drawing, to make connections 2.0 FXSQ24MVJU FXSQ30MVJU 60 208 230 FXSQ36MVJU Max 253 Min 187 FXSQ48MVJU MCA:Min Circuit Amps (A); MFA:Max ... this installation manual, install drain piping in order to ensure proper drainage and insulate piping in order to prevent condensation • Improper drain piping may result in water leakage and property...
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Bảng chức năng nhiệm vụ và sơ đồ tổ chức phòng kế toán
Ngày tải lên :
18/10/2013, 16:15
... Tham m u cho lãnh đạo Công ty nguồn vốn giá dự to n l m sở ký kết hợp đồng với đối tác Thu thập xử lý thông tin có liên quan đến nghiệp vụ kinh tế tài phát sinh Công ty Đ m bảo nguồn vốn cho m t ... tài bí m t kinh doanh công ty Tham m u cho Tổng Gi m đốc trình đề xuất chế độ thi đua khen thưởng, kỷ luật nâng bậc lương cán bộ, nhân viên Công ty Thực số chức khác Tổng Gi m đốc giao Nhi m vu:ï ... doanh m y tổ chức Công ty - Chấp hành đònh Ban ki m soát việc ki m tra hoạt động kế to n tài - p dụng khoa học quản lý tiên tiến vào công tác kế to n, bồi dưỡng nghiệp vụ chuyên m n cho cán kế to n,...
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GIẢI PHÁP NHẰM TẬN DỤNG TỐT NHẤT NGUỒN VỐN FDI VÀO LĨNH VỰC PHÂN PHỐI BÁN LẺ VIỆT NAM
Ngày tải lên :
19/10/2013, 17:20
... tố tạo nên gia tăng m nh tiêu dùng nước Việt Nam Sự thay đổi cách thức mua s m người tiêu dùng Việt Nam diễn m nh n m qua cho thấy xu hướng chuyển dịch m nh từ việc mua s m khu chợ truyền thống ... cho lần mua s m tăng 20% Theo dự đoán, m ời n m tới, số tăng lên tương ứng 90% Thành phố Hồ Chí Minh 50% Hà Nội Điều cho thấy thay đổi nhanh m nh cách thức người dân Việt Nam việc mua s m hàng ... t m kinh doanh thương m i tầng lên đến 200 USD /m2 /tháng Hiện công suất thuê m t thành phố lớn nước ta đạt 95% nhu cầu thuê m t tăng m nh Có nhiều trung t m thương m i đến trình xây dựng nhà kinh...
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Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf
Ngày tải lên :
15/02/2014, 01:20
... periplasmic protein in P pantotrophus m plas Cyto e bran Mem sm Perip la lysa luble Inso MW kDa t m plas Cyto e bran Mem sm B Perip la cell Tota l luble Inso MW kDa t lysa A cell te Interestingly, ... strains and plasmids used in this study are detailed in Table Paracoccus pantotrophus strains were grown in Luria–Bertani (LB) medium or in a defined minimal medium [26] supplemented with 20 mm ... pantotrophus extracts and western blotting Paracoccus pantotrophus strains were grown in L cultures of minimal media supplemented with 20 mm sodium nitrate and 20 mm sodium succinate and harvested at...
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Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt
Ngày tải lên :
18/02/2014, 16:20
... ribosome [21] Two subunits make up this 2.3 ·106 Da particle [22] The small 30S, or S, subunit contains 21 proteins involved in mRNA binding The 50S, or L, subunit contains 34 proteins, binds to tRNA, ... to tRNA, and mediates peptidyl transfer Shown in Fig 4A is the base peak chromatogram from a 90 min, automated, on-line LC-MS ⁄ MS experiment performed on a benchtop LTQ instrument modified for ... with trypsin, and phosphopeptides were enriched from the sample using immobilized metal affinity chromatography and analyzed using nanoflow HPLC interfaced to a QLT mass spectrometer (Thermo Electron...
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Tài liệu Báo cáo khoa học: Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines pptx
Ngày tải lên :
19/02/2014, 05:20
... C-LytA monitored by near-UV CD with no ligand added (—) and upon addition of ligands: s, d, 2.5 and 20 mM choline; n, m, 2.5 mM and 20 mM atropine; h, j), 2.5 mM and 20 mM ipratropium (B) Intrinsic ... choline; d, 50 mM choline; m, 1.5 mM atropine; h, 1.5 mM ipratropium FEBS Journal 274 (2007) 364–376 ª 2006 The Authors Journal compilation ª 2006 FEBS 369 Inhibition of choline-binding proteins ... the points are shown Table Thermal stabilization of C-LytA by ligands Ligand added tm (°C) Control (free C-LytA) Choline 2.5 mM Atropine 2.5 mM Ipratropium 2.5 mM Choline 20 mM Atropine 20 mM Ipratropium...
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Tài liệu Báo cáo khoa học: Aldehydes release zinc from proteins. A pathway from oxidative stress⁄lipid peroxidation to cellular functions of zinc pptx
Ngày tải lên :
19/02/2014, 06:20
... of mm acrolein, all seven zinc ions are released within h Aldehydes and zinc metabolism Fig Acrolein releases zinc from metallothionein (MT) The kinetics of zinc transfer from MT isoform (MT2) ... cadmium and zinc To prepare zinc MT2 [56], mg of MT2 was dissolved in mL of 20 mm Tris ⁄ HCl (pH 7.4) containing 50 mg dithiothreitol, and incubated at 25 °C for 24 h After incubation, the sample ... Journal compilation ª 2006 FEBS 4303 Aldehydes and zinc metabolism Q Hao and W Maret Aldehydes induce expression of metallothionein in HepG2 cells A cadmium-binding assay was used to examine...
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Tài liệu Báo cáo khoa học: The small heat shock proteins and their role in human disease pptx
Ngày tải lên :
19/02/2014, 18:20
... their interactions with one another, with membranes [13,29], and with cell components such as actin [30] and the intermediate filament proteins CP49 and filensin [31] a-crystallins maintain lens ... formation of huntingtin-containing inclusion bodies in Huntington’s disease encourages cell survival, whereas monomers and small inclusion bodies of huntingtin, a protein possessing abnormal ... condition involving brain and spinal cord in ammation T cells from MS patients express a dominant response to aB-crystallin, a major autoantigen affiliated with central nervous system myelin, the disease...
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Tài liệu Báo cáo khoa học: Analyzing changes of chromatin-bound replication proteins occurring in response to and after release from a hypoxic block of replicon initiation in T24 cells pptx
Ngày tải lên :
21/02/2014, 00:20
... Electrophoresis of proteins and Western blotting Cytosolic and nucleosolic proteins were precipitated from the respective supernatants by adding five volumes of icecold acetone Proteins remaining in Triton-extracted ... (50 mM Hepes, pH 7.5, 100 mM KCl, 0.25% Triton X-100, 2.5 mM MgCl2, mM dithiothreitol) containing Ó FEBS 2003 3882 M van Betteraey-Nikoleit et al (Eur J Biochem 270) aprotinine (1 lM), leupeptine ... envelope and complete extraction Supernatants were combined and yielded nucleosolic proteins The remaining pellet contains all DNA and structure bound proteins and is further referred to as chromatin-fraction...
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Báo cáo khoa học: Functional classification of scaffold proteins and related molecules pptx
Ngày tải lên :
06/03/2014, 22:21
... (adaptors), larger multidomain proteins targeting and regulating more proteins in complex ways (scaffolds ⁄ anchoring proteins) and proteins specialized to initiate signaling cascades by localizing ... SH2 ⁄ SH3 domain-containing adaptors link downstream target molecules to the membrane-bound receptor In some cases, the adaptor may recruit binding partners directly to the plasma membrane through ... lipid-binding domain In hematopoietic cells, pleckstrin homology domain (PH)-containing adaptor molecules provide important links between phosphoinositide3 kinase and lymphocyte function For example,...
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Báo cáo khoa học: Compartmentalized signalling: Ras proteins and signalling nanoclusters pdf
Ngày tải lên :
07/03/2014, 00:20
... Ras isoforms traffic from the ER–Golgi to the plasma membrane and endosomes on membranes, the lability of palmitoylation provides a mechanism for cytosolic recycling back to the Golgi complex The ... 19292–1 9300 Conclusion Compartmentalized Ras signalling enables a spectrum of outputs to be tuned from a minimal core-signalling machinery by modulating access to a variety of activating and effector ... 14-kilodalton protein interacts with the mitogen-activated protein kinase scaffold mp1 on a late endosomal ⁄ lysosomal compartment J Cell Biol 152, 765–776 43 Torii S, Kusakabe M, Yamamoto T, Maekawa M...
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Báo cáo khoa học: In vivo RNA interference in oyster – vasa silencing inhibits germ cell development pptx
Ngày tải lên :
07/03/2014, 00:20
... sequence amplified by real-time PCR primers, so as to avoid any bias from the injected dsRNA when quantifying Oyvlg mRNA In our preliminary experiments, no differences were observed in response to injection ... important technical advance for examining the first crucial step in successfully using in vivo RNAi: the introduction of dsRNA into animal tissues than the spermatocyte stage Conversely, spermatids ... occurring during germ cell development Knockdown of Oyvlg mRNA and protein expression A 70% inhibition of mRNA level after dsRNA treatment was considered to be a threshold for effective RNAi [23] In...
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Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
Ngày tải lên :
07/03/2014, 12:20
... can be adopted Combinatorial labelling minimizes the number of samples that need to be prepared and analyzed in order to obtain the same information as that obtained from a much larger set of ... transaminases and glutamine synthase [20] Among the multitude of metabolic enzymes present in the cell extract, only those leading to transfer of [15N]-amino groups to other amino acids can interfere ... by the amide cross-peaks in the [15N]-HSQC spectrum Remaining free [15N]-amino acids are equally unproblematic because the amino protons of amino acids exchange too rapidly at neutral pH to yield...
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Báo cáo khoa học: ` Inhibition of human ether a go-go potassium channels by 2+ Ca ⁄calmodulin binding to the cytosolic N- and C-termini potx
Ngày tải lên :
07/03/2014, 12:20
... J Mol Biol 301 , 1237–1256 Ikura M (1996) Calcium binding and conformational response in EF-hand proteins Trends Biochem Sci 21, 14–17 Chin D & Means A (2000) Calmodulin: a prototypical calcium ... consisting of six transmembrane segments and extended cytosolic N- and C-terminal domains Functional channels are formed by of such subunits involving the C-terminal assembly domain (C) Magnified ... structural information on the cytosolic channel domains in complex with CaM Initiated by the finding that hEAG1 channels lacking the entire N-terminal cytosolic domain are insensitive to Ca2+ ⁄ CaM we...
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Báo cáo khoa học: Computer-assisted mass spectrometric analysis of naturally occurring and artificially introduced cross-links in proteins and protein complexes potx
Ngày tải lên :
07/03/2014, 12:20
... post-translational modification patterns, disulfide bond structures, chemical modifications and cross-linking in protein mixtures, complexes, and assemblies Proteins A single protein, a list of proteins from a mixture ... deactivated for adding to a combined spectrum number ⁄ mass ⁄ intensity list, which is used for matching Data matching Data matching can be achieved in matching quests In each quest, matching criteria ... or a chemically induced cross-link (interpeptide cross-link products) Each mass in the combined mass list is matched within a custom defined mass window Computer-assisted mass spectrometric analysis...
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Báo cáo khoa học: Comprehensive sequence analysis of horseshoe crab cuticular proteins and their involvement in transglutaminase-dependent cross-linking potx
Ngày tải lên :
07/03/2014, 21:20
... obtained here may not be representative of all cuticular chitin-binding proteins in T tridentatus Amino acid compositions of high molecular mass (HMM) chitin-binding proteins HMM chitin-binding proteins ... HMM chitin-binding proteins cDNA fragments of HMM chitin-binding proteins were amplified by PCR using degenerate oligonucleotide primers based on amino acid sequences derived from intact proteins ... 6) In mammals, insulin-like growth factor-binding proteins, which contain the IGFBP motif, modulate the actions of the insulin-like growth factors in endocrine, paracrine, and autocrine systems...
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Báo cáo khoa học: Dynamin-related proteins and Pex11 proteins in peroxisome division and proliferation doc
Ngày tải lên :
07/03/2014, 21:20
... dynamins, the N-terminal GTPase domain, a middle domain, and the C-terminal GED (Fig 2) Obvious PRDs or PH domains are not found in DRPs Examples of DRPs are Mx proteins, mammalian DLP1, and ... domains of effector proteins of the actin cytoskeleton Dynamins are required in phagocytosis and in caveolae- and clathrin-dependent endocytosis [71] Of the three conventional mammalian dynamins, ... symbiont-derived cytosolic factors has been lost Two of the three yeast DRPs, Dnm1 and Mgm1, are involved in mitochondria morphology and inheritance Mgm1 is required for mitochondrial inner membrane fusion...
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Báo cáo khoa học: A novel mechanism of TGFb-induced actin reorganization mediated by Smad proteins and Rho GTPases docx
Ngày tải lên :
16/03/2014, 06:20
... Cumming J, Miano JM, Wang DZ, Li HJ & Li L (2005) Myocardin enhances Smad3-mediated transforming growth factorbeta1 signaling in a CArG box-independent manner: Smad-binding element is an important ... Recombinant mature human TGF-b1 was purchased from R&D Systems Inc (Minneapolis, MN, USA) Restriction enzymes and modifying enzymes (T4 DNA ligase and alkaline phosphatase) were purchased from Minotech ... 24 h in the absence or presence of the general inhibitor of transcription actinomycin D, and changes in the actin cytoskeleton were monitored by fluorescence using rhodamine phalloidin staining...
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Báo cáo khoa học: Cellular response to unfolded proteins in the endoplasmic reticulum of plants pptx
Ngày tải lên :
16/03/2014, 11:20
... N-terminal bZIP DNA-binding domain, a putative transmembrane domain, and a 56-amino-acid small C-terminal domain (Fig 1A) A truncated form of AtbZIP60 lacking the transmembrane domain (AtbZIP60 DC) ... Similar to stromal cell derived factor-2 Similar to anther ethylene-up-regulated calmodulin-binding protein ER1 Similar to growth factor protein with mutT domain GST Similar to AHB1 Putative ring ... COPII-mediated sorting of membrane proteins into ER subdomains is essential for its entry into the proteasomal degradation pathway In plants, a similar sorting system for membrane proteins is...
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