lysophosphatidic acid as a novel lipid mediator
DEVELOPMENT OF e SRS (ENVIRONMENT SENSING RESPONSE SYSTEM) AS a NOVEL METHOD TO DISTINGUISH GENETIC ENVIRONMENTS AND RESOLVE CLOSELY RELATED NUCLEIC ACID SEQUENCES
... strains of Malaria parasites (Plasmodium falciparum) and common serotypes of Dengue viruses A computational algorithm was created that greatly automated the design and assessment of these Mz-based ... countries where Malaria is most devastating [Mens et al, 2007] As such, PCR based methods are excluded from consideration as a field-ready rapid diagnostic kit for Malaria [McNamara et al, 2004] In ... pPLG RCA RCAA RS RTA SB Nucleic Acid Sequence-Based Amplification An isothermal amplification technique that amplifies DNA or RNA into RNA Nucleic acid Non-targeted Sequence Typically an RNA sequence...
Ngày tải lên: 04/10/2015, 15:52
Báo cáo khoa học: Identification of mitogen-activated protein⁄extracellular signal-responsive kinase kinase 2 as a novel partner of the scaffolding protein human homolog of disc-large docx
... mutagenic primers were: GRRF-PDZ1: 5¢GAAAGGGGAAATTCAGGGCGTCGT TTCAGCATTGCAGGAGG-3¢; GRRF-PDZ2: 5¢-ATTA AAGGTCCTAAAGGTCGTCGGTTTAGCATTGCTGGA GG-3¢; RAAV-MEK2: 5¢-CACCCACGCGCGCCGCCGT GTGA-3¢ and ... RT-PCR using PlatiniumÒ Taq DNA High Fidelity Polymerase (Invitrogen) and the primers: PDZ-1-2, forward: 5¢-CCGAATTCGAAGAAATCACACTTGAAAGG-3¢, and reverse: 5¢GGATCCCCATCATTCATATACATACTTGT GGGTT-3¢; ... 5¢-GTGGGGAAATATGCTCTTGAGGAGGT-3¢; primers 2, forward: 5¢-GTGACTTCAGAGACACTGCCA-3¢, and reverse: 5¢-CCCTTTCAAGTGTGATTTCTTC3¢; primers 3, forward: 5¢-ACCAGATGGTGAGAGCGAT-3¢, and reverse: 5¢-CTGTCTTTCATAGGTCCCAAT-3¢...
Ngày tải lên: 22/03/2014, 16:20
Báo cáo Y học: Repression of FasL expression by retinoic acid involves a novel mechanism of inhibition of transactivation function of the nuclear factors of activated T-cells pptx
... total RNA using 100 ng random hexamer (Pharmacia, Uppsala, Sweden) The PCR primer sequences used were as follows FasL (forward: 5¢-ATGTTTCAGC TCTTCCACCTACAGAAGGA-3¢, reverse: 5¢-CAGAGA GAGCTCAGATACGTTGAC-3¢); ... Jurkat cells and the induction was repressed by all-trans-RA treatment in a dose-dependent manner (Fig 1A) FasL transcription was decreased at all-trans-RA concentrations as low as 0.01 lM, and ... repression was observed with all-trans-RA concentrations as low as 0.1 lM, and NFAT binding was abolished in the presence of 1.0 lM all-trans-RA In contrast, SP-1 binding was similar at all concentrations...
Ngày tải lên: 24/03/2014, 03:21
Báo cáo khoa học: Identification of the N-terminal region of TjZNT2, a Zrt⁄Irt-like protein family metal transporter, as a novel functional region involved in metal ion selectivity ppt
... -MASSPTKILCDAGESDLCRDDAAAFLLKFVAIASIL 1:MFFIDVLWKLFPLYLFGSERDYLSETESILKIVPETMAAASSLSILCDAGEPDLCRDDSAAFLLKLVAIASIF TjZNT1 TjZNT2 37:LAGVAGVAIPLIGKNRRFLQTEGNLFVAAKAFAAGVILATGFVHMLAGGTEALTNPCLPDYPWSKFPFPGFFA ... Thlaspi caerulescens as a model system Ann Bot 102, 3–13 Weber M, Harada E, Vess C, Roepenack-Lahaye E & Clemens S (2004) Comparative microarray analysis of Arabidopsis thaliana and Arabidopsis halleri ... transport FEBS Lett 581, 2263–2272 N-terminus of TjZNT2 is involved in ion selectivity Tomatsu H, Takano J, Takahashi H, Watanabe-Takahashi A, Shibagaki N & Fujiwara T (2007) An Arabidopsis thaliana...
Ngày tải lên: 29/03/2014, 00:20
Báo cáo khoa học: Neuropeptide S as a novel arousal promoting peptide transmitter pdf
... sensory nucleus and the lateral parabrachial nucleus of the brainstem A small number of scattered NPS-positive cells were found in other brain areas, such as amygdala and hypothalamus The NPS-producing ... produce a transient increase in intracellular free Ca2+, indicating that NPS might be an excitatory transmitter in vivo by elevating intracellular Ca2+ A radiolabled analog of NPS (125I-labeled ... profound increase in locomotion, measured as the total distance traveled over one hour It is well known that animals naturally show increased exploratory activity when they are exposed to a novel environment...
Ngày tải lên: 30/03/2014, 11:20
Báo cáo hóa học: " A pilot clinical trial testing mutant von HippelLindau peptide as a novel immune therapy in metastatic Renal Cell Carcinoma" doc
... IL-2 and IFN -a for months as an adjuvant therapy; patient received IFN -a for lung metastasis, and patient received highdose IL-2 for metastatic mediastinal lymphadenopathy followed by radical lymph ... subcutaneously as easy and practical as they may be–reverse gradually as soon as vaccinations are completed Accordingly, we believe that such treatment needs to be continued in order to maintain ... manuscript EA participated in the patients care RI carried out the immunoassays AT carried out the immunoassays BG participated in the patients care VEH participated in the patients care WML analyzed...
Ngày tải lên: 18/06/2014, 16:20
Báo cáo hóa học: " Designed hybrid TPR peptide targeting Hsp90 as a novel anticancer agent" docx
... peptide After incubation, an assay for cell viability was carried out using Living Cell Count Reagent SF (Nacalai Tesque) according to the manufacturer’s protocol Absorbance was measured at a wavelength ... then analyzed by phase-contrast (DIC), fluorescence (TAMRA-red) or merge image (DIC and TAMRA-red) All images were taken using confocal laser scanning microscopy as described in Methods All scale ... RQIKIWFQNRRMKWKKKAYAAAGNSYFK (Antp-TPR mutant 1), RQIKIWFQNRRMKWKKKAYARIGNSGGG (Antp-TPR mutant 2), and RQIKIWFQNRRMKWKKRKFSAAIGYNKY (Antp-scramble) Materials Anti-Hsp90 and anti-Hsp70 antibodies, human recombinant...
Ngày tải lên: 18/06/2014, 16:20
Báo cáo sinh học: " Body fluid derived exosomes as a novel template for clinical diagnostics" pptx
... AAA GA-3’; GAPDH fwd 5’-ACA CCC ACT CCT CCA CCT TT-3’, GAPDH rev 5’-TGC TGT AGC CAA ATT CGT TG-3’ To compare and quantify different measurements a cellular cDNA was used as standard and the amount ... esRNA was analyzed by PCR (C) Total RNA was isolated from amniotic fluid and urine exosomes and analyzed via an Agilent Bioanalyzer The results show that exosomes contain variable amounts of 18 and ... 0.5 0.0 pg RNA relative to cellular standard 0.8 GAPDH 0.6 0.4 0.2 0.0 exosomes 10 11 12 membrane blebs A as e N R + -R N as as N R + R + e A A e A e as N as N -R + R N as e e A A B 320 bp 382...
Ngày tải lên: 18/06/2014, 19:20
báo cáo hóa học:" Body fluid derived exosomes as a novel template for clinical diagnostics" ppt
... AAA GA-3’; GAPDH fwd 5’-ACA CCC ACT CCT CCA CCT TT-3’, GAPDH rev 5’-TGC TGT AGC CAA ATT CGT TG-3’ To compare and quantify different measurements a cellular cDNA was used as standard and the amount ... esRNA was analyzed by PCR (C) Total RNA was isolated from amniotic fluid and urine exosomes and analyzed via an Agilent Bioanalyzer The results show that exosomes contain variable amounts of 18 and ... 0.5 0.0 pg RNA relative to cellular standard 0.8 GAPDH 0.6 0.4 0.2 0.0 exosomes 10 11 12 membrane blebs A as e N R + -R N as as N R + R + e A A e A e as N as N -R + R N as e e A A B 320 bp 382...
Ngày tải lên: 20/06/2014, 03:20
Báo cáo y học: "Screening of an endothelial cDNA library identifies the C-terminal region of Nedd5 as a novel autoantigen in systemic lupus erythematosus with psychiatric manifestations" ppt
... the ELISA experiments and participated in analysis of data CA performed the statistical analysis and the clinical associations AS participated in the analysis and interpretation of data and in ... study, participated in its design and coordination, and drafted the manuscript All authors read and approved the final manuscript Acknowledgements An association between serum AECAs and psychosis ... gelatin (1:100 for total IgG) and pipetted onto plates at 100 µl per well Plates were incubated for hour at 20°C and washed as described Peroxidase-conjugated goat antihuman IgG (Bio-Rad) was...
Ngày tải lên: 09/08/2014, 06:23
Báo cáo y học: "Identification of kinectin as a novel Behçet''''s disease autoantigen" doc
... by screening an aplastic anemia patient for candidate antigens using a Clontech human fetal liver cDNA expression library and it was concluded that seven out of 18 aplastic anemia patients were ... anti-kinectin and anti-PMS1 antibodies in Japanese aplastic anaemia patients Br J Haematol 2005, 128:221-223 39 Imai H, Nakano Y, Kiyosawa K, Tan EM: Increasing titers and changing specificities of antinuclear ... 1996, 7:1015-1024 Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs Nucleic Acids Res 1997,...
Ngày tải lên: 09/08/2014, 07:20
Báo cáo y học: "Small RNA sequencing reveals miR-642a-3p as a novel adipocyte-specific microRNA and miR-30 as a key regulator of human adipogenesis" ppt
... genome (release hg19) accounted for miRNAs annotated in mirBase (release 16) Other small RNA species, such as piwi-interacting RNAs (piRNAs) and small nucleolar RNAs (snoRNAs), were also identified ... correspond to any annotated small RNA Our small RNA cloning strategy only captures small RNAs that are, as miRNAs, 5’-phosphorylated and, Page of 13 thus, eliminates RNA degradation products generated ... renilla and firefly luciferase activities were assayed with the Dual Glo Luciferase Assay System (Promega) and measured with a luminometer (Luminoskan Ascent, Thermo Scientific, Waltham, MA, USA)...
Ngày tải lên: 09/08/2014, 23:20
Báo cáo y học: " Systems biology coupled with label-free high-throughput detection as a novel approach for diagnosis of chronic obstructive pulmonary disease" pptx
... vision, as others have also adopted this strategy as a way forward in molecular analysis Alagaratnam et al are utilising Bayesian approaches to pursue muscular dystrophy diagnosis [223] Similarly, ... other arbitrary measures for disease classification Adopting a systems biology approach, whereby a disease defining molecular fingerprint is analysed, would increase the accuracy of disease diagnosis, ... 51(12):2333-2340 Okamoto M, Kawabe T, Iwasaki Y, Hara T, Hashimoto N, Imaizumi K, Hasegawa Y, Shimokata K: Evaluation of interferon-gamma, interferon-gamma-inducing cytokines, and interferongamma-inducible...
Ngày tải lên: 12/08/2014, 14:20
Identification and characterization of protein kinase CK2 as a novel interacting protein of neuronal CDK5 kinase and its functional role in microtubule dynamics
... ECL enhanced chemiluminescence EDTA ethylenediaminetetra acetic acid EGTA ethyleneglycoltetra acetic acid ER endoplasmic reticulum FAK focal adhesion kinase FCS fetal calf serum FPLC fast protein ... neurofilaments and the phosphorylation is required in maintaining axonal morphology Cdk5 was originally isolated from the brain as a neurofilament kinase to catalyze the KSP phosphorylation at the tail ... co-immunoprecipitation approach in our search for novel interacting proteins Using the former approach, the catalytic α subunit of protein kinase CK2 (formerly known as casein kinase 2) was isolated from rat...
Ngày tải lên: 16/09/2015, 17:10
Signaling pathway inhibitor library screening reveals b catenin TCF4 as a novel telomerase regulator in cancer cell lines
... inhibition of ataxia telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) protein kinases though detail mechanism has yet being elucidated (d'Adda di Fagagna et al 2003; Takai et al 2003) Telomeres ... have an exposed 53BP1 interaction site thereby activates the DNA damage surveillance pathway TRF2 was proposed as an ATM inhibitor as it was found to be able to physical interacts with ATM at ... telomerase inhibitors They vary in form of screening targets as well as the screening approaches A summary of telomerase targeted cancer therapy and screening that has being published is available...
Ngày tải lên: 02/10/2015, 17:14
Identification of plant as a novel and alternative host model for burkholderia pseudomallei
... GGATCCGATCGTGTCGAACGAGATCA 60.0 KHWTTSS2P3 GGATCCGGCATCGACGGTATTCT 66.9 KHWTTSS2P4 AAGCTTATATCGCCGGGATAGCGTA 66.9 BTTTSS2P1 aaGAATTCGGTGGCCTCCAGAAACAGT 55.0 BTTTSS2P2 aaGGATCCGAGGACCCGCATGACAAG 55.0 ... GAATTCCGAACCGCTTTGTGATAACC KHWTTSS1P2 GGATCCGATCTTGAGCAGATGCTTG 60.7 KHWTTSS1P3 GGATCCGCCACGATATCCTCGAAAAG 60.7 KHWTTSS1P4 CTGCAGAGCTACGCCGTGAACGTATT 60.7 KHWTTSS2P1 GAATTCCTCGAACCGTCCATCGTC 60.0 KHWTTSS2P2 GGATCCGATCGTGTCGAACGAGATCA ... koalas, kangaroos, deers, cats, dogs and gorillas (Sprague and Neubauer, 2004) Cases in animals have been reported in several countries including Australia, China, Thailand, Iran, Saudi Arabia, South...
Ngày tải lên: 09/10/2015, 10:49
Probiotics as a novel approach to modulate incretins, insulin secretion and risk factors of type 2 diabetes and complications
... GAC AGA ATG TAC ACT TCA 24 nt tuf-S CCA TCA AGC CGC ACA CCA AGT TCG 24 nt Lacto-F2 TGG AAA CAG ATG CTA ATA CCG 21 nt Lacto-R2 CGT CCA TTG TGG TAG ATT CCC T 22 nt Lacto-S CTG AGA CAC GGC CCA WAC ... clinical trial, assigned according to the randomization list The appropriate package was handed to the particular participant Statistical data analysis and power calculation Statistical data analysis ... WAC TCC TAC GG 26 nt F_reut_IS ACC GAG AAC AAC GCG TTA TTT 21 nt R_reut_IS CAT AAC TTA ACC TAA ACA ATC AAA GAT TGT 30 nt P_reut_IS ATC GCT AAC TCA ATT AAT 18 nt Ampl Lit 159 bp (Yang et al., 2002)...
Ngày tải lên: 19/11/2015, 16:47
báo cáo hóa học: " Methyl salicylate 2-O-b-D-lactoside, a novel salicylic acid analogue, acts as an antiinflammatory agent on microglia and astrocytes" docx
... Iannitelli DAE, Cataldi A, Zara S, Nasuti C, Di Stefano A: Ibuprofen and Glutathione Conjugate as a Potential Therapeutic Agent for Treating Alzheimer’s Disease Arch Pharm (Weinheim) 2010 Ray B, Lahiri ... Neuroinflammation, represented by activated microglia and astrocytes, is a prominent pathological feature that contributes to neurodegeneration in AD In AD brain, activated microglia release a variety ... effect of ascorbic acid and acetylsalicylic acid on prostaglandin E2 release in primary rat microglia J Neurochem 2003, 86:173-178 14 Candelario-Jalil E, de Oliveira AC, Graf S, Bhatia HS, Hull...
Ngày tải lên: 19/06/2014, 22:20
Novel design of a compacted micro-structured air-breathing PEM fuel cell as a power source for mobile phones
... 0) and condensation ( m phase < 0) is assumed Where m phase is mass transfer: for evaporation & & & & ( m phase = mevap ) and for condensation ( m phase = mcond ) (kg/s) So that the mass balance ... water is the same as the gas phase temperature 2.2.2 Gas diffusion layers The physics of multiple phases through a porous medium is further complicated here with phase change and the sources and ... field, mass transport and electrochemistry in an air-breathing cathode of a planar PEM fuel cell In their results, electrochemical/mass characteristics such as flow velocities, species mass fraction,...
Ngày tải lên: 05/09/2013, 14:58
Tài liệu Báo cáo khoa học: Bacterial-induced hepoxilin A3 secretion as a pro-inflammatory mediator pptx
... SipA [24] The Salmonella effector protein, SipA, promotes a lipid signal transduction cascade that recruits an ADP-ribosylation factor guanine nucleotide exchange factor (such as ARNO) to the apical ... ARNO) to the apical plasma membrane ARNO facilitates ADP-ribosylation factor activation at the apical membrane, which in turn stimulates phospholipase D recruitment to and activity at this site The ... responsible for the membrane release of arachidonic acid (AA), the precursor of HXA3 Once liberated within the cytosol, AA is available as a substrate for 12-lipoxygenase (12-LOX), the enzyme...
Ngày tải lên: 19/02/2014, 02:20