ly49c nk cells are expanded by ad5il 12 therapy but cannot be further enhanced by combination therapy
Báo cáo khoa học: Plant oxylipins: Plant responses to 12-oxo-phytodienoic acid are governed by its specific structural and functional properties ppt
Ngày tải lên :
16/03/2014, 02:20
... have to be covalently linked to the molecules by transacylations From this, a general involvement of transacylation reactions, introducing oxylipins into galactolipids, cannot be excluded beyond ... poorly promoted by indole-3-acetic acid Thereby, the velocity of the gravitopic movement is seemingly accelerated [51–54] The majority of the effects described by the Nick group are most likely ... regulators are also involved in the redox-dependent transmission of oxylipin signals Very recently, PHO1;H10, a member of the PHO1 gene family of Arabidopsis thaliana, has been described 4698 as being...
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Báo cáo khoa học: Complex gangliosides are apically sorted in polarized MDCK cells and internalized by clathrin-independent endocytosis doc
Ngày tải lên :
16/03/2014, 04:20
... pCEFL-SialT-2-HA) has been described previously by Daniotti et al [53] GalNAc-T-myc (3¢ end of the human GalNAc-T cDNA epitope tagged with human c-myc; pCIneo-GalNAc-T-myc) has been described previously by Giraudo ... GD3 and GM1 gangliosides are preferentially transported to the apical surface of polarized MDCK cells GD3 and GM1 gangliosides are rapidly endocytosed in MDCK cells It has been observed in different ... Eps15-expressing cells This results indicate that clathrin-coated vesicles not participate in the endocytic process of GD3 in MDCK cells A B C Fig GD3 and GM1-CTx are internalized in MDCK cells by clathrin-independent...
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báo cáo hóa học:" Luteinizing hormone-induced Akt phosphorylation and androgen production are modulated by MAP Kinase in bovine theca cells" doc
Ngày tải lên :
20/06/2014, 07:20
... the theca cells awaits further investigation Competing interests As a mechanism explaining why phospho-Akt content in theca cells was increased only after 12 h of incubation with LH, we are also ... phosphorylation in theca cells, are subjects that are currently under investigation in our laboratory The authors declare that they have no competing interests Authors' contributions SF, MO, KT, KH, ... this study was substantiated by the secretion of estradiol; prepared theca cells did not produce estradiol in the presence or absence of forskolin, whereas granulosa cells obtained from the same...
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Báo cáo y học: "B cells from rheumatoid arthritis patients show important alterations in the expression of CD86 and FcgRIIb, which are modulated by anti-tumor necrosis factor therapy" potx
Ngày tải lên :
12/08/2014, 12:20
... Arthritis Research & Therapy 2010, 12: R68 http://arthritis-research.com/content /12/ 2/R68 before adalimumab treatment was started (Figure 4b), but the difference between naïve and memory B cells was still ... of B cells in the lymphocytic population (mean percentage ± SD 7.5 ± 3.1 and ± 2.2, respectively) Furthermore, when we discriminated B cells in naïve cells, memory cells and plasmablasts by means ... Catalán et al Arthritis Research & Therapy 2010, 12: R68 http://arthritis-research.com/content /12/ 2/R68 Page of 11 A Naïve B cells Memory B cells Plasmablasts * 12 25 20 75 % CD86 % CD86 % CD86...
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Tài liệu Báo cáo khoa học: Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide pptx
Ngày tải lên :
18/02/2014, 13:20
... 0.25 0.5 12 24 5000 Ctr 0.25 0.5 12 24 5000 3500 3000 2500 2000 1500 1000 500 (h) LPS Ctr 0.25 0.5 12 24 25 000 0.25 0.5 12 24 0.25 0.5 12 24 0.0 B TNF-α (pg·mL–1) 100 ng·mL–1 LPS 0.25 0.5 12 24 ... expression of mRNA RGS1 160 140 Poly(I:C) 120 100 80 60 40 20 Ctr 0.5 12 Rel expression of mRNA RGS1 3.0 S Riekenberg et al 1.2 Rel expression of mRNA RGS are modulated by lipopeptides and LPS Poly (I:C) ... FSL-1 12 10 Rel expresssion of mRNA Rel expresssion of mRNA RGS are modulated by lipopeptides and LPS S Riekenberg et al RGS1 LPS Wild-type MyD88–/– TRIF–/– Wild-type MyD88–/– TRIF–/– Ctr 0.5 12...
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Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc
Ngày tải lên :
19/02/2014, 00:20
... febrile response induced by SEC1 is dependent on NADPH oxidase and not on PI3K ⁄ Akt or 5-LOX ⁄ 3640 FLAP because these phenomena are attenuated by apocynin and not by ETYA, MK 886, Wort or HIMO ... excess of the unlabeled jB oligonucleotide (Fig 2B, lane 9) Conversely, a 100-fold excess of oligonucleotide probes of the unlabeled mutant jB (Fig 2B, lane 10) or the unlabeled AP-1 (Fig 2B, ... T cells has been suggested to proceed in part via SHIP-1mediated phosphorylation of IKK complex or via Sykdependent phosphorylation of IjB-a [36] In stimulated phagocytic cells, ROS produced by...
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Tài liệu Báo cáo khoa học: The distinct nucleotide binding states of the transporter associated with antigen processing (TAP) are regulated by the nonhomologous C-terminal tails of TAP1 and TAP2 ppt
Ngày tải lên :
20/02/2014, 02:21
... Sequences were retrieved from the GenBank database (GenBank X57523 and X63854) and aligned using the software VECTOR NTI (Informax) Identical residues are marked by black boxes while grey boxes indicate ... that were mutually exchanged between TAP1 and TAP2 are underlined in black In addition, the amino acid sequence encoded by exon 11 (GenBank AL732652) is underlined by a dashed black line (residues ... and quantitated by gamma counting [25] For peptide crosslinking permeabilized cells were incubated with lM radioiodinated and HSAB-conjugated peptide S8 Crosslinking was induced by irradiation...
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Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf
Ngày tải lên :
20/02/2014, 11:20
... formed by labelled fgl2–97/)77 probe, but not by the mutated fgl2–97/)77 oligo (lane 8–10) These results suggest that complex A is formed by DNA binding proteins functionally related to members ... SVE-10 endothelial cells were incubated with probes shown in Table and Fig Arrows indicate the bands that are interacting with these probes All the lanes have labelled probes The adding of extracts ... nuclear extracts (lane 2) These complexes were competed away by the same unlabelled probe (lane 3) but not by an unrelated nonspecific probe (lane 4) The addition of anti-Sp1 IgG1 shifted the slowest...
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Tài liệu Báo cáo Y học: Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis docx
Ngày tải lên :
22/02/2014, 04:20
... 1), concentrated and mixed with 12 mL Emulsifier SafeTM Microsomes from germinating soybean (Glycine max L cv Weber) plantlets were prepared from g seeds as described [14] They were resuspended ... soybean (Fig 1B), but other molecular species are eluted at times which allow a perfect distinction between the two sets of PtdIns molecules: the C16:0/C17c + C18:1/C17c and C16:0/C19c peaks are ... of PtdIns but also the exchange reactions in the absence or in the presence of CMP In plants, the exchange reaction is known to occur [7,8] but it has never been shown to be catalysed by the same...
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The Neighbors Are Watching by Debra Ginsberg pot
Ngày tải lên :
05/03/2014, 10:20
... fairgrounds are now filled to capacity i just got the reverse 911 call telling me to be ready to evacuate from solana beach i believe the evac sites are supposed to be “protected,” but i have no ... have no idea October 22, 2007 6:55 PM Laura said Thanks, Dell I think that makes sense re the fairgrounds—it is surrounded by wetlands Good luck to you on the evac be safe October 22, 2007 ... October 29, 2007 9:40 AM Sarasmom said What a terrible thing to say! What if something happened to her? The parents are probably reading this right now You should be ashamed of yourself October...
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Why Government Bonds are sold by Auction and Corporate Bonds by Posted-Price Selling pot
Ngày tải lên :
06/03/2014, 04:21
... should be sold by auction and the latter by PPS What is more, corporate bonds should be sold at a discount Both predictions appear to be borne out by the evidence: primary debt issues are sold by ... GOVERNMENT BONDS ARE SOLD BY AUCTION AND CORPORATE BONDS BY POSTED-PRICE SELLING In most industrialized countries, government bonds are sold by auction whereas corporate bonds are sold by posted-price ... acquiring information are likely to be dominated by auctions is suggested by the results of Harstad (1990) and Bulow and Klemperer (1996) Harstad (1990) shows that entry costs are borne by the seller...
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Default Risk Cannot Explain the Muni Puzzle: Evidence from Municipal Bonds That Are Secured by U.S. Treasury Obligations ppt
Ngày tải lên :
15/03/2014, 03:20
... indexed by t The estimates are compared across maturities and ratings Trzcinka finds that none of the estimated β’s are significantly different from (1 − τc ) at the 5% significance level Furthermore, ... probabilities are between 1.5 and 3% Furthermore, there are theoretical reasons to believe that default risk will cause the term structure to have a steeper slope For example, Kim, Ramaswamy, and Sundaresan ... price bucket labels describe the percentage of observations for the year that have a month-end bond price within the range described by the column label Nobs is the average number of monthly...
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No Safer Place in the World for Your Money - How to Make Sure All Your Deposits Are Protected by FDIC Insurance docx
Ngày tải lên :
15/03/2014, 10:20
... to think about carefully because there could be unanticipated consequences “For example,” Becker explained, “changing a single account without beneficiaries to a testamentary account with beneficiaries ... own one account at a bank — a joint account with a balance of $400,000 — all of that money would be insured because each person’s share (here presumed to be $200,000) would be protected for up to ... resources are strong Your insured deposits are absolutely safe.” To learn more about how to be sure all your deposits are under the insurance limits and fully protected by the FDIC, see the article below...
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Báo cáo khoa học: Vanadium-induced apoptosis of HaCaT cells is mediated by c-fos and involves nuclear accumulation of clusterin pptx
Ngày tải lên :
16/03/2014, 02:20
... cell death by interacting with and interfering with Ku70–Ku80 [21,22] Considering that epidermal cells are mostly exposed to environmental insults, and that both AP-1 [10 12] and CLU [20] are induced ... c-fos cells than in HaCaT Neo cells (Fig 4C, upper panel, arrow) To further confirm that c-fos overexpression was directly related to the observed apoptotic outcome of HaCaT cells (which bear mutant ... HaCaT nCLU (C120) cells strongly expressed a doublet of 49 kDa and two smaller fragments of 26 and 20 kDa, corresponding to nCLU (C120) (Fig 6A, lane 2), as compared with HaCaT NeoT cells (Fig...
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Báo cáo khoa học: Hansenula polymorpha pex11 cells are affected in peroxisome retention potx
Ngày tải lên :
16/03/2014, 04:20
... gene has been deposited in GenBank (accession number DQ645582) Cells of a constructed PEX11 deletion strain (pex11) grew like the wild-type on glucose (not shown), but displayed a minor but significant ... Inp1–GFP distribution in vivo in pex11 cells by time-lapse imaging, using wild-type cells as control These studies indicated that in the wild-type control cells, Inp1–GFP fluorescence is often below the ... microscopy revealed that in wild-type (WT) cells, peroxisomes are evenly distributed over the mother cell and developing bud (A) In pex11 cells, peroxisomes are predominantly present in the daughter...
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Báo cáo khoa học: Krit 1 interactions with microtubules and membranes are regulated by Rap1 and integrin cytoplasmic domain associated protein-1 doc
Ngày tải lên :
16/03/2014, 05:20
... the pellet (P) were analyzed by SDS ⁄ PAGE and quantified by fluorometry Protein precipitation in the absence of liposomes has been substracted Bands below Krit1 band are E coli contaminants that ... microtubule and lipid binding ´ S Beraud-Dufour et al Fig YFP-Krit1 colocalizes with CFP-labelled microtubules in transfected BHK cells and is delocalized by activated Rap1 BHK cells were transfected with ... pMT2-HA-Rap1V12 Twenty-four hours after transfection, cells were fixed in 4% paraformaldehyde for 20 and processed for immunofluorescence analysis as already described [41] HA-Rap1V12 was labelled with...
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Báo cáo khoa học: The properties of phosphodiesterase 11A4 GAF domains are regulated by modifications in its N-terminal domain pptx
Ngày tải lên :
16/03/2014, 06:20
... levels is currently unclear, partly because of a lack of any clear pattern in its tissue distribution and partly because a PDE11-specific inhibitor has not yet been developed In studies using a ... corroborated the aforementioned conclusions because PDE11A4(198-568)cyaB1 AC could not be stimulated by cGMP, whereas PDE11A4(197-568)cyaB1 AC was stimulated 3.4-fold by cGMP with an EC50 value of 7.6 ... of the nine truncated constructs (Fig 2C), although the reduction appeared to be minor By and large, we consider this to be within the experimental variability for this type of assay Matters...
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Báo cáo Y học: Expression and distribution of penaeidin antimicrobial peptides are regulated by haemocyte reactions in microbial challenged shrimp pptx
Ngày tải lên :
18/03/2014, 01:20
... described below In situ hybridization Probes A plasmid containing pen-3a cDNA (GenBank accession number Y14926) was used as template for the preparation of the probes Digoxigenin (DIG)-UTPlabelled ... observations suggest that penaeidins are expressed either by maturating stem cells or by haemocytes early before leaving the haematopoietic tissues However, it cannot be excluded that circulating haemocytes ... number of silver grains counted for every haemocyte by the autoradiography BIOCOM software Silver grains are produced by contact of 35 S-emission with the autoradiographic emulsion The number...
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Báo cáo khóa học: Large aggregating and small leucine-rich proteoglycans are degraded by different pathways and at different rates in tendon pot
Ngày tải lên :
23/03/2014, 12:20
... radioactivity of radiolabelled sulfate present in or produced by the explant cultures will be considerably reduced, thereby decreasing the level of re-use of [35S]sulfate by the cells of the cultures ... 95% of 35S-labelled proteoglycans remained in the matrix by the end of the culture period on day 15 The work described above suggests that small proteoglycans are taken up by the cells and digested ... analyzed by size exclusion chromatography on Sephadex G-25 as described above All the radiolabelled material appearing in the total volume of the columns was shown to be free [35S]sulfate because...
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Báo cáo khóa học: Soluble LDL-R are formed by cell surface cleavage in response to phorbol esters pdf
Ngày tải lên :
23/03/2014, 12:20
... receptor number, at least in HepG2 cells, can be regulated not only by transcriptional control but also by enhanced turnover in response to unknown stimuli that signal via PKC This enhanced turnover ... was upregulated by replacing medium with MEM containing LPDS (2.5 mgÆmL)1) and culturing for a further 12 h at 37 °C [24] Cells were then metabolically labeled with [35S]methionine by incubating ... After 36 h, LDL-R activity was upregulated by incubating cells in MEM/LPDS (2.5 mgÆmL)1) for a further 12 h Surface LDL-R activity was measured by incubating cells with mL ice cold MEM/LPDS buffered...
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