... in mega4 (Tamura et al 2004) Chemicals l-Tryptophan, meat extract, perchloric acid, ortho-phosphoric acid, nitric acid and ethanol were obtained from the Himedia Laboratories, Mumbai, India All ... fructose and starch) and amino acids (l-glycine, l-alanine, l-valine, l-leucine, l-isoluceine, l-phenylalanine, l-tyrosine, l-tryptophan, l-aspartic acid, l-glutamic acid, l-histidine, l-arginine, ... ortho-phosphoric acid and ml of Salkowski’s reagent followed by incubation for 30 in dark, at room temperature The developed pink colour was measured by recording the absorbance at 530 nm using UV–Vis...
Ngày tải lên: 06/08/2013, 21:06
... lyso-phosphatidic acid acyltransferase (LPAT) crucial for trierucin bioassembly, and the seed-specific FAE1, crucial for erucic acid biosynthesis Earlier, we reported on the expression of yeast LPAT gene ... remaining aqueous phase was then acidified with M HCl Free fatty acids were extracted in hexane, the solvent removed under a stream of N2, and the free fatty acids were transmethylated in M methanolic ... one at position 118 with asparagine instead of aspartic acid, while at the position 484 in Hero, aspartic acid is substituted by a glutamic acid residue HEA B rapa FAE1 has a serine residue at position...
Ngày tải lên: 08/03/2014, 09:20
Reductive Cleavage of N-O Bonds in Hydroxylamines and Hydroxamic Acid Derivatives Using Samarium Diiodide pot
... reactions using SmI2 prompted us to examine the reductive cleavage of the N-O bond of N-benzyl-N-acetylhydroxamic acid (Table 3, Entries and 2) Typically hydroxamic acids are cleaved using Mg(Hg)/TiC14 ... N-acetylated O-alkylhydroxylarnines at lower temperatures resulted in an increased yield (Entries and 7) Thus, the carbonyl substituent allows for reduction at lower temperatures Utilization of N-acetylated ... trifluoroaceto-hydroxamic acid derivative Despite the large number of efficient methods for N-O bond reduction, we encountered limitations of the more common procedures Using our previously developed...
Ngày tải lên: 22/03/2014, 18:20
SỰ BIẾN ĐỘNG HÀM LƯỢNG PHYTIC ACID TỪ CÁC DÒNG LÚA ĐƯỢC GÂY ĐỘT BIẾN BẰNG CHIẾU XẠ TIA GAMMA pptx
... tiếng phytic acid thấp Os -lpa- XQZ-1, Os -lpa- XS110-1, Os -lpa- XS110-2, Os-lpaMH86-1, Os -lpa- Z9B-1,… giống Os -lpa- XS110-1/GO133 xác định gen điều khiển tính trạng phytic acid thấp (lpa- 1) lại định ... lúa chín tiến hành thu hạt trữ mẫu để phân tích phytic acid 2.2.3 Phương pháp phân tích hàm lượng phytic acid hạt Kỹ thuật phân lập đột biến phytic acid thấp nhiều loại trồng khác thực gián tiếp ... Các dòng lại thể lượng phốt vô hay phytic acid hạt đa phần mức 3.2 Sự biến động hàm lượng phytic acid quần thể OM4900 sau chiếu xạ Kết phân tích hàm lượng phytic acid 100 dòng thuộc quần thể sau...
Ngày tải lên: 25/03/2014, 07:20
Pore Characterization in Low-k Dielectric Films Using X-ray Reflectivity: X-ray Porosimetry pot
... nearly absorbance (pure airflow), each successive curve indicates a 10 sccm increment in the flow rate of the toluene-saturated air and a 10 sccm decrement in the pure airflow rate The ratio of the ... thin, low- k dielectric films 1.B The Concept of X-ray Porosimetry In the following, we describe a form of porosimetry with the requisite sensitivity to quantify the small pore volumes in a thin, low- k ... ρe of PS is also comparable to the average ρe of most candidate low- k materials The µ for PS will be slightly lower than most low- k materials since the latter typically contain Si and O, elements...
Ngày tải lên: 27/06/2014, 10:20
Enhancing humic acid removal using surface modified granular media
... disrupted porcine cartilage using polarized Raman spectroscopy; J Biomed Opt 2011;16(1):017003017003-10 doi:10.1117/1.3528006] ! #+! Fig 15 Schematics (redrawn) showing acid etching of mineralized ... ;:
Ngày tải lên: 05/10/2015, 21:24
Báo cáo y học: "Monte Carlo Commissioning of Low Energy Electron Radiotherapy Beams using NXEGS Software"
... per SSD, a total of 10 = 1350 simulations) using 5000000 Monte Carlo histories each, again following the recommendations in the NXEGS documentation Using the same processor as before, we find ... calculated and measured dose distributions along seven different dimensions as described below, essentially following the recommendations of Van Dyk et al [7] Note that each quantity represents a difference ... generator initialization To make this investigation tractable, we confine our attention to simulations using applicators with only standard inserts Once the behavior of NXEGS is well understood for these...
Ngày tải lên: 03/11/2012, 10:05
Customer satisfaction that using low price airlines
... Normally, LCCs provide basic air transport service with no frills and lower fares, and the average LCC fare is about 40–60% lower than a typical FSC fare (Lawron, 2002; Oliveira, 2008) However, ... non-reserved seating LCCs are gaining substantial economies through fleet commonality, low distribution cost using the internet, point-to-point service, and non-refundable tickets Hence, LCCs in ... South Korean LCCs also emerged after deregulation and liberalization in South Korea, using the strategies of low fares and service quality In addition, regression of domestic air service by South...
Ngày tải lên: 07/08/2013, 22:38
Tài liệu Báo cáo khoa học: Investigation and prediction of the severity of p53 mutants using parameters from structural calculations pptx
... sets The mutants were divided into two classes Mutants with an activity above 25% were considered to be less severe and were denoted class mutants (524 mutants) , whereas those with lower activity ... energy on the y-axis For severe mutants, the number of mutants increases at high energy values, causing a gap between the curves representing severe and nonsevere mutants The separation is not ... case, only 45% of the mutants are classified Hotspot mutants There are several p53 mutants that are extremely overrepresented in human cancers, for example three lung cancer mutants induced by smoking...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Metabolic flux profiling of Escherichia coli mutants in central carbon metabolism using GC-MS docx
... 600 nm (D600) Glucose concentrations were determined enzymatically using a commercial kit (Beckman, Palo Alto, CA, USA) The following physiological parameters were determined during the exponential ... eV GC-MS raw data were analyzed using the software package MassLab (Fisons), avoiding detector overload and isotope fractionation as described [20] The amino acids analyzed by GC-MS were aspartate, ... reversibility Solid arrows indicate precursor withdrawal for the amino acid analyzed by GC-MS Inactivated proteins in the investigated knockout mutants are highlighted in boxes Abbreviations: 6PG, 6-phosphogluconate;...
Ngày tải lên: 20/02/2014, 23:20
Báo cáo khoa học: Unraveling multistate unfolding of pig kidney fructose-1,6bisphosphatase using single tryptophan mutants docx
... activity disappears It is important to note that the four mutants remain in the tetrameric state and not aggregate at low concentrations of GdmCl (lower than 0.9 m) as revealed by the size-exclusion ... and purification of FBPase mutants Replacement of phenylalanine residues with tryptophan was carried out using the Altered sites II in Vitro Mutagenesis System kit, following the manufacturer’s ... C1–C2 interface Results Catalytic and spectroscopic properties of tryptophan mutants of FBPase The single tryptophan mutants, Phe16Trp, Phe89Trp, Phe219Trp and Phe232Trp FBPases exhibited identical...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Mutants of Saccharomyces cerevisiae deficient in acyl-CoA synthetases secrete fatty acids due to interrupted fatty acid recycling docx
... yeast and consists of myristic acid (14:0), palmitic acid (16:0), palmitoleic acid (16:1), stearic acid (18:0) and oleic acid (18:1) (see Fatty acid secretion in mutants of yeast supplementary ... extracellular fatty acids In addition, we determined the amount of lipid-bound fatty acids to cover all major pools of fatty acids (Fig 1B,D) The amount of cellular free fatty acids could be easily ... FEBS 2767 Fatty acid secretion in mutants of yeast A M Scharnewski et al Fig Relationship between fatty acid concentration and stage of the culture (A) Extracellular free fatty acids in the culture...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx
... most mutants of Q143A hMnSOD, presumably displaying unchanged or lower catalytic activity compared to the original Q143A hMnSOD enzyme, could not confer efficient growth to E coli cells, whereas mutants ... visual inspection Mutants that clearly displayed more rapid growth than Q143A hMnSOD mutants in the paraquat-containing media were selected for further characterization, and other mutants were discarded ... hMnSOD gene Amino acid substitutions (capital letters) and base pair substitutions (small letters) are indicated Recurring amino acid substitutions are indicated by bold type Mutants obtained...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Optimization of D-amino acid oxidase for low substrate concentrations – towards a cancer enzyme therapy pdf
... library of DAAO mutants was then generated in BL21(DE3)pLysS E coli cells [16] For the identification of DAAO mutants with increased enzymatic activity at low O2 concentrations, the following screening ... inactive (see below) Kinetic properties Steady-state measurements The dependence of the catalytic activity of the DAAO mutants on the oxygen and d-Ala concentrations was assessed using the enzyme-monitored ... enzyme at low concentrations of both O2 and d-Ala (see below) An accurate determination of steady-state parameters according to the method of Gibson [22] is, however, not possible at low O2 concentration,...
Ngày tải lên: 16/03/2014, 02:20
Báo cáo khoa học: Weak organic acid stress inhibits aromatic amino acid uptake by yeast, causing a strong influence of amino acid auxotrophies on the phenotypes of membrane transporter mutants ppt
... to weak organic acid stress Auxotrophic requirements for aromatic amino acids dramatically increase sensitivity to weak organic acid stress, a sensitivity suppressed by amino acid supplementation ... indicated level of organic acid The plates were photographed after 3d at 30 °C whether trp1-D63, and other mutations leading to requirements for aromatic amino acids, might be causing an unusually high ... supplemented with intermediates (e.g shikimate) that allow aromatic amino acid synthesis to occur independently of the transketolase reaction [19] Weak acid stress in yeast is acting in a fundamentally...
Ngày tải lên: 23/03/2014, 21:20
Using lichen as bioindicator of air pollution: Acid deposition Monitoring and Assessment Third country Training pptx
... bioindicator of air pollution & acid deposition There are two alternative methods: Community changes: changes in species composition at different levels of pollution slow response Physiology changes: ... Brief history of using lichens as bioindicator of air pollution In 1866 it was noted that lichens disappeared from ... •Degradation of chlorophyll • Photosynthesis decline Provide faster responses to air pollution and acid deposition These are warning signals before severe damages occur, which are difficult to correct...
Ngày tải lên: 29/03/2014, 18:20
Biodiesel production process by homogeneousheterogeneous catalytic system using an acid base catalyst
... attack, allowing the transesterification and esterification reaction by a well known homogeneous Brønsted acid catalysis mechanism [27] The acid esterification of free fatty acid by weak acid Brønsted ... 160–168 out using acid or basic heterogeneous catalysts or, better, heterogeneous catalyst with both acid and basic properties They could combine the advantages of the alkaline and acid transesterifications ... materials with acid base properties (K-MCM-41, K-ITQ-6) as catalysts for the transesterification of waste fruit oilseeds with high amount of free fatty acids (FFAs), higher than wt% (as low- quality...
Ngày tải lên: 05/05/2014, 08:34
Báo cáo sinh học: " Detection of virus mRNA within infected host cells using an isothermal nucleic acid amplification assay: marine cyanophage gene expression within Synechococcus sp" potx
... nucleic acid and 3WJ probes are removed, following binding in a streptavidin coated well, and RNA signal is detected via a colour change Quantification of signal takes place in a 96 well plate, allowing ... DNA and RNA were extracted as described below Extraction of viral nucleic acid from infected host cells RNA was extracted from mL of pelleted cells using a Qiagen RNeasy® Mini kit according to ... Specific detection of DNA and RNA targets using a novel isothermal nucleic acid amplification assay based on the formation of a three-way junction structure Nucleic Acids Research 2001, 29:e54 Levi K,...
Ngày tải lên: 18/06/2014, 18:20
Báo cáo hóa học: " Detection of virus mRNA within infected host cells using an isothermal nucleic acid amplification assay: marine cyanophage gene expression within Synechococcus sp" doc
... nucleic acid and 3WJ probes are removed, following binding in a streptavidin coated well, and RNA signal is detected via a colour change Quantification of signal takes place in a 96 well plate, allowing ... DNA and RNA were extracted as described below Extraction of viral nucleic acid from infected host cells RNA was extracted from mL of pelleted cells using a Qiagen RNeasy® Mini kit according to ... Specific detection of DNA and RNA targets using a novel isothermal nucleic acid amplification assay based on the formation of a three-way junction structure Nucleic Acids Research 2001, 29:e54 Levi K,...
Ngày tải lên: 20/06/2014, 01:20
Báo cáo hóa học: " Research Article Trade-Offs between Energy Saving and Reliability in Low Duty Cycle Wireless Sensor Networks Using a Packet Splitting Forwarding Technique" docx
... initialization procedure are reported in [1] We show below how nodes can be synchronized using the same IMs seen above It is worth mentioning that, using the proposed CRTbased scheme, a perfect synchronization ... network topologies and in the radio links conditions, causing serious problems related to the network reliability An interesting example of using a multipath approach together with erasure codes ... Figure 4) while the other messages follow (in the same cycle) Obviously, a maximum value of M exists in order to respect (3) Nevertheless, we can choose a low value of pDC to reduce the power...
Ngày tải lên: 21/06/2014, 11:20