... described [10 ]. The Cys residue was added to the C-terminal end of the 13 -amino-acid peptide corresponding to the primarysequence ofthe Tat protein to provide a sulfhydryl group for further ligation ... eitherwith the Antennapedia p eptide [11 ] or the Tat peptide [12 ± 14 ]. Along the same lines, antisense oligonucleotides (ON)were coupled chemically to the Antennapedia p eptide [1] , orto the ... omain is receptor-independent. J. Biol.Chem. 2 71, 18 188 18 193. 19 . Brugidou, J., Legrand, C., Me ry, J. & Rabie , A. (19 95) The retro-inverso form of a homeobox-derived short peptide is rapidlyinternalised...
... homooligomericGIRK1F137S) currents had been described previously [18 ]. To assess the role ofthe S ⁄ Ts in the regulation of GIRK1 via PKA in a manner that is unbiased by the eventual contributions ofthe other ... distalpart is unique for GIRK1 among the Kir3.x isoforms.Further truncations ofthe C-terminus revealed a 49amino acid stretch (amino acids 362– 411 ) that was the most prominent target for phosphorylation ... CaCl2was 1. 8 mmolặL )1 and 2.5 mmolặL )1 pyruvate and 0 .1% antibiot-ics (G -13 97; 10 00 stock from Sigma-Aldrich) were added;HK: 96 mmolặL )1 KCl, 2 mmolặL )1 NaCl, 1 mmolặL )1 MgCl2, 1 mmolặL )1 CaCl2,...
... the introduction which is concerned with presenting the rationale, the scope of the study, the aims ofthe study, the methods ofthe study and the design ofthe study.Part 2 is the body ofthe ... ofthe test of English conversation proficiency,Edgbaston, Birmingham B15 2TT United Kingdom. 16 . Naunton, J. (2002), Head For Business, Oxford University Press, Oxford. 17 . Nunan, D. (19 91) , ... the range ofthe test is 6. This is meant that the test involves the items that rankfrom the easiest to the most difficult ones and the test items, therefore, may cover the content ofthe course...
... alone in their discomfort in dealing with questions of scale. Economists are far worse: the vast majority of economists never evenbother to ask the question ofthe proper scale ofthe economy ... old-growth forest in the eastern United States. The Cathedral Pines preservein Connecticut contained about twenty-five acres of old-growth pine, one of the last examples of that type of forest in the ... appreciate the full meaning of thatinsight for years.In 19 67, MacArthur and Wilson oƒered the most complete version of their ideas in a book, The Theory of Island Biogeography. In it, they too,...
... Referral for treatment and/or completion of treatment for LTBI is not required forthe civil surgeon to sign the I-693 form. As regards TB, the signature indicates the applicant is freeof Class ... not involve the lungs. Completion of treatment for Class B1-Extrapulmonary TB is not required forthe civil surgeon to sign the I-693 form. As regards the TB, the signature indicates the applicant ... equivalent of 15 mg/day or more of prednisone for at least one month. o Have a history of organ transplant. ã Have signs or symptoms of active TB, regardless ofthe size of induration of the TST...
... in the family, ofthe family in the commonwealth, ofthe commonwealth in the continent, and of the continent in the world. Finally, the world itself at any moment was nomore than the mood of ... upon the spiritual faculties. Then, they have the use of all the churches exceptours, and all the Cathedrals; and they are beginning at last to encouragesentiment. Then, they may display their ... like the fall ofthe Monasteries underHenry VIII the same results, the same arguments, the same incidents.They were the strongholds of Individualism, as the Monasteries were the strongholds of...
... followed by incubation at 94 °C for 15 sand 68 °C for1 min for 40 cycles. For detection of SAF-3mRNA, nested PCR was performed. In the first PCR, the SAF-3-specific forward primer 5Â-CGCGAGCCACCTCCCTCCCTCC-3Â ... sterile dH2O; 1. 0 lL of thissuspension was used as the template forthe second PCR. The SAF-3 primers forthe second PCR primers were5Â-CCGCCATGGAT CCCAGCAACTGGAGCAGC-3Â(forward) and 5Â-GAGAACCGGGAGCAAGTCCAC-3Â(reverse). ... 2009, revised 12 May2009, accepted 5 June 2009)doi :10 .11 11/ j .17 42-4658.2009.0 713 6.x The Cys2His2-type zinc finger transcription factor serum amyloid A activa-ting factor 1 [SAF -1, also known...
... s )1 (range 9 .1 14 .0); the second a-helixpresented a mean of 13 .2 s )1 (range 11 .9 14 .2); the third a-helix presented a mean of 11 .3 s )1 (range 8 .19 – 13 .5); and, finally, amino acids in the loop regionpresented ... dynamics. J PhysChem 10 0, 13 293 13 310 .7 Freed EO (19 98) HIV -1 gag proteins: diverse functionsin the virus life cycle. Virology 2 51, 1 15 .8 Freed EO & Martin M (20 01) HIVs and their replica-tion. ... responsible, from a thermodynamic point of view, forthe binding ofthe monomeric species of CAC?We have previously discussed the variation in the free energy of binding as a function ofthe changes...
... mixture ofthe sinecurve of analogue and 0 /1 values of digital. It alsorefers to the integration of AV and IT. Ideienvisioned the VAIO as a platform for a networkservice over which Sony could offer ... benchmark for their brand image. The product design and the product planning—they have learned from us. SoSony is a very good target for them.” To him,Samsung was merely one ofthe suppliers ratherthan ... upgraded music and video features. Theseofferings have been very successful. By 2006,Sony had sold 10 0 million game platforms and 1 billion game cartridges. 16 Its portable PlayStation,PSP,...
... Statements forthe Fiscal Years Ended September 30, 2 012 and 2 011 (D2 012 -D000FP- 012 0.000) Objective: Determine whether the financial statements as of September 30, 2 012 and 2 011 , are presented ... Availability of Spare Parts forthe C-27/G222 (D2 012 -D000AT- 017 0.000) Objective: Determine whether the cost and availability of spare parts forthe C-27/G222 will allow for continued sustainability of ... Completeness, and Rights ofthe Department ofthe Navy's Ordnance x x Attestation ofthe Audit Readiness ofthe Existence, Completeness, and Rights ofthe Department ofthe Navy's Uninstalled...
... protein )1 )GDP 0.07 0.03 41 ± 18 0.37 ± 0 .15 38 ± 2ADP 7.4 ± 3.9 5.5 ± 0.8 0.5 ± 0 .17 53 ± 15 GTP 0. 016 ± 0. 01 4.5 ± 0.6 0.06 ± 0. 01 4.0 ± 0.3ATP 5.0 ± 1. 7 1. 6 ± 0.06 0.42 ± 0.2 10 ± 1 Entamoeba ... above). The true nucleotide substrate of PGKs is the Mg–ATPcomplex [10 ,11 ]. Therefore, the Kdvalues were deter-mined in the presence of saturating concentrations of MgCl2(Table 3). From these ... and the side chains of Leu 311 andVal339 (patch C) define the limits ofthe adenine-bind-ing site [15 ,18 ].It has been reported that N6 ofthe adenine ringestablishes a hydrogen bond with the...
... facetstructure ofthe elongated islands by simply re-placing the (1 5 9)/(5 1 9) facets with the (1 7 11 )/(7 1 11) at an angle of 19 ° with respect to the Fig. 3. STM images of Ge island growth on Si (1 1 ... in Fig. 1c. All of these observations lead to the conclusion that relaxation ofthe islands at thisgrowth stage is dominant, in the ẵ 11 10 and 1 11 0directions. Island growth in these two ... cut from a Si (1 1 3)wafer (P doped, 1 10 X cm) with a misorientationtowards the ẵ 11 10 direction of less than 0:2°.Gedeposition at 1. 4 ML/min, where 1 ML is definedas 8: 2 Â 10 14 atoms/cm2,...