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Ngày tải lên: 05/07/2014, 18:27
Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx
... described [10 ]. The Cys residue was added to the C-terminal end of the 13 -amino-acid peptide corresponding to the primary sequence of the Tat protein to provide a sulfhydryl group for further ligation ... either with the Antennapedia p eptide [11 ] or the Tat peptide [12 ± 14 ]. Along the same lines, antisense oligonucleotides (ON) were coupled chemically to the Antennapedia p eptide [1] , or to the ... omain is receptor-independent. J. Biol. Chem. 2 71, 18 188 18 193. 19 . Brugidou, J., Legrand, C., Me ry, J. & Rabie , A. (19 95) The retro- inverso form of a homeobox-derived short peptide is rapidly internalised...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: Identification of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase pdf
... homooligomeric GIRK1 F137S ) currents had been described previously [18 ]. To assess the role of the S ⁄ Ts in the regulation of GIRK1 via PKA in a manner that is unbiased by the eventual contributions of the other ... distal part is unique for GIRK1 among the Kir3.x isoforms. Further truncations of the C-terminus revealed a 49 amino acid stretch (amino acids 362– 411 ) that was the most prominent target for phosphorylation ... CaCl 2 was 1. 8 mmolặL )1 and 2.5 mmolặL )1 pyruvate and 0 .1% antibiot- ics (G -13 97; 10 00 stock from Sigma-Aldrich) were added; HK: 96 mmolặL )1 KCl, 2 mmolặL )1 NaCl, 1 mmolặL )1 MgCl 2 , 1 mmolặL )1 CaCl 2 ,...
Ngày tải lên: 07/03/2014, 00:20
Development of the Quantitative PCR Method for Candidatus ‘Accumulibacter phosphatis’ and Its Application to Activated Sludge
Ngày tải lên: 05/09/2013, 09:38
Validity of the achievement written test for non major, second year students at economics department, hanoi open university
... the introduction which is concerned with presenting the rationale, the scope of the study, the aims of the study, the methods of the study and the design of the study. Part 2 is the body of the ... of the test of English conversation proficiency, Edgbaston, Birmingham B15 2TT United Kingdom. 16 . Naunton, J. (2002), Head For Business, Oxford University Press, Oxford. 17 . Nunan, D. (19 91) , ... the range of the test is 6. This is meant that the test involves the items that rank from the easiest to the most difficult ones and the test items, therefore, may cover the content of the course...
Ngày tải lên: 06/02/2014, 14:45
Tài liệu THE FUTURE OF THE WILD Radical Conservation for a Crowded World ppt
... alone in their discomfort in dealing with questions of scale. Economists are far worse: the vast majority of economists never even bother to ask the question of the proper scale of the economy ... old- growth forest in the eastern United States. The Cathedral Pines preserve in Connecticut contained about twenty-five acres of old-growth pine, one of the last examples of that type of forest in the ... appreciate the full meaning of that insight for years. In 19 67, MacArthur and Wilson oƒered the most complete version of their ideas in a book, The Theory of Island Biogeography. In it, they too,...
Ngày tải lên: 17/02/2014, 17:20
FAQs Tuberculosis Component of the Technical Instructions (TIs) for the Medical Examination of Aliens in the United States doc
... Referral for treatment and/or completion of treatment for LTBI is not required for the civil surgeon to sign the I-693 form. As regards TB, the signature indicates the applicant is free of Class ... not involve the lungs. Completion of treatment for Class B1-Extrapulmonary TB is not required for the civil surgeon to sign the I-693 form. As regards the TB, the signature indicates the applicant ... equivalent of 15 mg/day or more of prednisone for at least one month. o Have a history of organ transplant. ã Have signs or symptoms of active TB, regardless of the size of induration of the TST...
Ngày tải lên: 06/03/2014, 04:20
Lord of the World docx
... in the family, of the family in the commonwealth, of the commonwealth in the continent, and of the continent in the world. Finally, the world itself at any moment was no more than the mood of ... upon the spiritual faculties. Then, they have the use of all the churches except ours, and all the Cathedrals; and they are beginning at last to encourage sentiment. Then, they may display their ... like the fall of the Monasteries under Henry VIII the same results, the same arguments, the same incidents. They were the strongholds of Individualism, as the Monasteries were the strongholds of...
Ngày tải lên: 06/03/2014, 15:20
Báo cáo khoa học: SAF-3, a novel splice variant of the SAF-1/MAZ/Pur-1 family, is expressed during inflammation pptx
... followed by incubation at 94 °C for 15 s and 68 °C for 1 min for 40 cycles. For detection of SAF-3 mRNA, nested PCR was performed. In the first PCR, the SAF-3-specific forward primer 5Â-CGCGAGCCACCT CCCTCCCTCC-3Â ... sterile dH 2 O; 1. 0 lL of this suspension was used as the template for the second PCR. The SAF-3 primers for the second PCR primers were 5Â-CCGCCATGGAT CCCAGCAACTGGAGCAGC-3Â (forward) and 5Â-GAGAACCGGGAGCAAGTCCAC-3Â (reverse). ... 2009, revised 12 May 2009, accepted 5 June 2009) doi :10 .11 11/ j .17 42-4658.2009.0 713 6.x The Cys2His2-type zinc finger transcription factor serum amyloid A activa- ting factor 1 [SAF -1, also known...
Ngày tải lên: 07/03/2014, 02:20
Báo cáo khoa học: Structural mobility of the monomeric C-terminal domain of the HIV-1 capsid protein pptx
... s )1 (range 9 .1 14 .0); the second a-helix presented a mean of 13 .2 s )1 (range 11 .9 14 .2); the third a-helix presented a mean of 11 .3 s )1 (range 8 .19 – 13 .5); and, finally, amino acids in the loop region presented ... dynamics. J Phys Chem 10 0, 13 293 13 310 . 7 Freed EO (19 98) HIV -1 gag proteins: diverse functions in the virus life cycle. Virology 2 51, 1 15 . 8 Freed EO & Martin M (20 01) HIVs and their replica- tion. ... responsible, from a thermodynamic point of view, for the binding of the monomeric species of CAC? We have previously discussed the variation in the free energy of binding as a function of the changes...
Ngày tải lên: 07/03/2014, 06:20
Báo cáo khoa học: Interactions of HIPPI, a molecular partner of Huntingtin interacting protein HIP1, with the specific motif present at the putative promoter sequence of the caspase-1, caspase-8 and caspase-10 genes pdf
... 0.05 0.00 0.02 0.06 0.04 [DNA] àM [DNA] àM 1. 50E-008 1. 40E-008 1. 30E-008 1. 30E-008 1. 28E-008 1. 26E-008 1. 32E-008 0 .11 0 0 .10 5 0 .10 0 0.095 0.090 0.085 0.080 1. 20E-008 1. 10E-008 1. 00E-008 1/ F 340 1/ F 340 1/ F 025 50 75 10 0 1/ [DNA] àM 20 25 30 35 40 45 50 1/ [DNA] àM 0 11 22334455 1/ [DNA] àM 0.08 Probe (9 ... GST-Hippi 10 9 8 7 6 F 340 5 4 3 2 1/ F 0 .14 0 0 .14 5 III 0 .13 5 0 .13 0 0 .12 5 1/ F 0 .15 0.20 0.25 0 .10 0.05 0.00 0.00 0. 01 0.02 [DNA] àM 0.03 0.04 0.05 010 20 1/ [DNA] àM 30 40 50 60 70 0 10 20 1/ [DNA] àM 30 40 50 Fig. ... (6XHN-HIPPI_pDED) K d =0.34n M C1ups 717 bp (HIPPI-Nterm) C1ups 60bp (HIPPI-pDED) 9 8 7 6 5 Fluorescence 305 4 3 2 1 0 0.00 0. 01 0.02 III 0.03 0.04 1/ ΔF 0 .18 0 .19 0.20 0. 21 0 .17 0 20 40 60 8 010 0 12 0 14 0 60 bp Fig....
Ngày tải lên: 07/03/2014, 10:20
Sony vs. Samsung: The inside story of the electronics' giants battle for global supremacy
... mixture of the sine curve of analogue and 0 /1 values of digital. It also refers to the integration of AV and IT. Idei envisioned the VAIO as a platform for a network service over which Sony could offer ... benchmark for their brand image. The product design and the product planning—they have learned from us. So Sony is a very good target for them.” To him, Samsung was merely one of the suppliers rather than ... upgraded music and video features. These offerings have been very successful. By 2006, Sony had sold 10 0 million game platforms and 1 billion game cartridges. 16 Its portable PlayStation, PSP,...
Ngày tải lên: 14/03/2014, 14:02
Office of the Deputy Inspector General for Auditing FY 2013 Audit Plan ppt
... Statements for the Fiscal Years Ended September 30, 2 012 and 2 011 (D2 012 -D000FP- 012 0.000) Objective: Determine whether the financial statements as of September 30, 2 012 and 2 011 , are presented ... Availability of Spare Parts for the C-27/G222 (D2 012 -D000AT- 017 0.000) Objective: Determine whether the cost and availability of spare parts for the C-27/G222 will allow for continued sustainability of ... Completeness, and Rights of the Department of the Navy's Ordnance x x Attestation of the Audit Readiness of the Existence, Completeness, and Rights of the Department of the Navy's Uninstalled...
Ngày tải lên: 15/03/2014, 23:20
Báo cáo khoa học: Molecular basis of the unusual catalytic preference for GDP/GTP in Entamoeba histolytica 3-phosphoglycerate kinase doc
... protein )1 ) GDP 0.07 0.03 41 ± 18 0.37 ± 0 .15 38 ± 2 ADP 7.4 ± 3.9 5.5 ± 0.8 0.5 ± 0 .17 53 ± 15 GTP 0. 016 ± 0. 01 4.5 ± 0.6 0.06 ± 0. 01 4.0 ± 0.3 ATP 5.0 ± 1. 7 1. 6 ± 0.06 0.42 ± 0.2 10 ± 1 Entamoeba ... above). The true nucleotide substrate of PGKs is the Mg–ATP complex [10 ,11 ]. Therefore, the K d values were deter- mined in the presence of saturating concentrations of MgCl 2 (Table 3). From these ... and the side chains of Leu 311 and Val339 (patch C) define the limits of the adenine-bind- ing site [15 ,18 ]. It has been reported that N6 of the adenine ring establishes a hydrogen bond with the...
Ngày tải lên: 16/03/2014, 01:20
Báo cáo khoa học: Kinetic study of the HIV)1 DNA 3¢-end processing Single-turnover property of integrase docx
... Single-turnover kinetics of HIV -1 integrase FEBS Journal 273 (2006) 11 37 11 51 ª 2006 The Authors Journal compilation ª 2006 FEBS 11 51 determination of the catalytic constant (k cat  k cat ), the K m value ... Single-turnover kinetics of HIV -1 integrase FEBS Journal 273 (2006) 11 3 711 51 ê 2006 The Authors Journal compilation ê 2006 FEBS 11 41 0.23 min )1 ), suggesting that the lag phase corresponds to the DNA-binding ... this 0 0.05 0 .1 0 .15 0.2 0.25 010 2030 r A 0 50 10 0 15 0 200 250 300 0.0 0.5 1. 0 1. 5 2.0 2.5 Time (min) P[ roduct n(] M) I II III N 1 2 T S P 0 10 15 60 90 12 0 15 0 18 0 240 300 5 30 S P 0 50 10 0 15 0 200 250 300 0.0 0.5 1. 0 1. 5 2.0 2.5 Time...
Ngày tải lên: 16/03/2014, 13:20
Influences of the si(1 1 3) anisotropy on ge nanowire formation and related island shape transition
... facet structure of the elongated islands by simply re- placing the (1 5 9)/(5 1 9) facets with the (1 7 11 )/ (7 1 11) at an angle of 19 ° with respect to the Fig. 3. STM images of Ge island growth on Si (1 1 ... in Fig. 1c. All of these observations lead to the conclusion that relaxation of the islands at this growth stage is dominant, in the ẵ 11 10 and 1 11 0 directions. Island growth in these two ... cut from a Si (1 1 3) wafer (P doped, 1 10 X cm) with a misorientation towards the ẵ 11 10 direction of less than 0:2°.Ge deposition at 1. 4 ML/min, where 1 ML is defined as 8: 2 Â 10 14 atoms/cm 2 ,...
Ngày tải lên: 16/03/2014, 15:34