lna as diagnostic probe or antisense agent

... for chorismate mutase activity in Escherichia coli An E coli strain (KA12) was engineered in which the genes encoding the bifunctional enzymes chorismate mutase–prephenate dehydratase and chorismate ... his 75th birthday We thank Katherina Vamvaca for helpful comments on the manuscript Financial support for the work on chorismate Ó FEBS 2004 mutases was provided by the ETH-Zurich and the Schweizer ... Biol 7, 470–479 10 Kast, P., Asif-Ullah, M., Jiang, N & Hilvert, D (1996) Exploring the active site of chorismate mutase by combinatorial mutagenesis and selection: the importance of electrostatic...

Ngày tải lên: 19/02/2014, 12:20

... vector for a phage l integrase mutant was set as 100% In each case, data were collected from six separate transfection assays, each employing two wells containing about  105 cells (C) Normalized ... dimethylsulfoxide, was tested at 100 mM In order to inactivate the TRE-CMV promoter, doxycyclin (20 ng:mL21) was added weeks prior to electroporation b-Galactosidase (b-gal) assays, Southern blotting ... product vectors were cointroduced with an expression vector for either Cre (pPGKCrebpa) or gd102NLS (pPGKgd102NLS) Substrate and product vectors cotransfected with an expression vector for the phage...

Ngày tải lên: 22/02/2014, 07:20

... essential for recognition of DNA by M.EcoRII as was suggested for several other MTases, as base ipping probably occurs with any base at the target position [26] However, in the case of the M.EcoRII ... Mikhailov for preparation of 2-pyrimidinone phosphoramidite, S Muller for oligoă nucleotide synthesis, S Klimas auskas for M.HhaI, A S Bhagwat for plasmid pT71 used for construction of a hybrid plasmid ... 2-pyrimidinone containing DNA as a MTase inhibitor 2-pyrmidinone incorporation in DNA sequences may also serve as a specic probe for studying discrimination contacts formed by proteins and functional...

Ngày tải lên: 07/03/2014, 15:20

... them & who has read them Words, phrases, sentences, paragraphs and books are datastructures capable of accumulating memories When these memories plug into a distributed intelligence (networked software), ... in text editors for pattern matching, in compilers for lexical analysis, in web browsers for html parsing, and in operating systems for graphical user interfaces They also serve as the control ... are perceived as alive Therefore the more mediated language becomes, the more it will seem alive Eventually its abstract foundation may be forgotten PROSTHESIS Related software case-study: Mr...

Ngày tải lên: 07/03/2014, 17:20

... kinds of stories they liked as children, or the fascination of ghost stories and fairy stories Moreover, some students will probably have some stories they would like to tell or favorite characters ... context Ask your students to listen for one or two specific words, play a tape recording of a short passage (two to three minutes at most), and ask for guesses about the meaning of the words Have ... to speaking or listening The central text for each lesson is literary Passages are selected from authors such as Mark Twain, George Orwell, Charles Dickens, or modern writers such as Chinua Achebe...

Ngày tải lên: 19/03/2014, 08:20

... sample or each dose standard was added to each well that was coated with purified anti-mouse haptoglobin and this was incubated for 15 o at 22 C Following four aspirations and washings with washing ... differentially-expressed plasma protein of mice exposed to DON was investigated, and the haptoglobin precursor in the blood plasma in combination with the Igs was found to be a diagnostic indicator for DON intoxication ... APPs producing organs was stimulated by pro-inflammatory cytokines such as IL-6, IL-1 and TNF-α, as well as by malnutrition [1,11,19] DON was observed to upregulate pro-inflammatory cytokines...

Ngày tải lên: 07/08/2014, 20:23

... saw )ASU ,tneligA( nevo nmuloc a dna ,)ASU ,tneligA( relpmasotua na ,)ASU ,tneligA( ressaged a ,)ASU ,tneligA( pmup a ,)ASU ,tneligA( rotceted VU yarra edoidotohp a gnidulcni metsys CLPH eroborcim ... roirp erutarepmet moor ta dewaht erew selpmas amsalp nezorF gnilpmas fo skeew nihtiw dezylana litnu Co02− ta nezorf deniatniam dna derevocer saw amsalp eht dna nim rof )aeroK ,nipsorciM linaH( g 007,61 ... erew selpmas laireS )%2.0 noitartnecnoc lanif( tnalugaocitna na sa ATDE gniniatnoc ebut citsalp a otni )ASU ,nosnikciD-notceB( reniatucav a gnisu niev liat eht morf detcelloc erew selpmas doolb...

Ngày tải lên: 07/08/2014, 23:22

... classification by the experts was considered the 'gold standard' for fever aetiology, against which PCT measurement was evaluated Procalcitonin assay For serum PCT measurement, we used a time-resolved ... completed a standardized form for each patient, including co-morbidity, vital signs, putative source of infection, prior antibiotic therapy before ED consultation, and presence or absence of headache, ... myalgia, sore throat, rhinorrhoea, dry cough, polyadenopathy and rash The form ended with the emergency physician's diagnostic suspicion, antibiotic prescription and patient course (not admitted or...

Ngày tải lên: 13/08/2014, 03:21

... concentrations For the determination of procalcitonin concentration, the Liaison Brahms PCT assay (Diasorin S.p.A., Sallugia, Italy) was used C-reactive protein was measured by using the CRP wide-range assay ... associated with organ dysfunction As a mechanism, an increase in plasminogen activator inhibitor (PAI-1), which is produced by endothelium and liver, has been demonstrated [18] As activated protein ... are given as mean and standard error of the mean (SEM), as well as median and 25th and 75th percentiles The Shapiro-Wilk test excluded a normal distribution for several values Therefore, the Mann-Whitney...

Ngày tải lên: 13/08/2014, 21:21

... nor advisable for use in the general population at large [7,8] As the authors of the current study point out [5], the high mobility group box-1 protein (HMGB1) has been known for many years as ... released from cells, in which extracellular form it can bind to an inflammatory receptor called Receptor for Advanced Glycan Endproducts (RAGE) Activated macrophages and monocytes secrete HMGB1 as ... http://www.sjtrem.com/content/19/1/28 For one, the search for new and better diagnostic biomarkers would potentially have great impact on workup and use of diagnostic imaging if accurate and predictive of disease In particular...

Ngày tải lên: 13/08/2014, 23:20

... acid sensors and APS sensors was very similar to that of 1-decene sensors To theorise the changes in impedance the PSi was modelled as a series of parallel plate capacitors with the pores depicting ... defined as the accessible area of solid surface to a gas (eg: N2) per unit mass of material SSA increases with decreasing dimensions of the pores i.e from macro pores to micro pores as shown ... feasibility of employing the bio-molecule immobilized PSi as a platform for a label free bio-sensor (immuno sensor) and (vi) Finally, to investigate the theory behind the characteristic performance...

Ngày tải lên: 11/09/2015, 09:59

Ngày tải lên: 27/08/2016, 11:01

... ( Word Reader - Unregistered ) www.word-reader.com never turn a blind eye to the situations of them Therefore, I believe that donating money on these poor people is much more meaningful ... We can never know when we have to use a large amount of money Therefore, the best way is to keep the money in our accounts for those possible incidents in the future With enough savings, we will ... with panic when encounter accidents, we can make our dreams come true, and help those who need our assistance ...

Ngày tải lên: 27/08/2016, 11:01

Ngày tải lên: 27/08/2016, 11:01

Ngày tải lên: 27/08/2016, 11:02

... dangerous for a economic entity On the contrary, if everyone spends as much as they can, then more money will come to companies, which will hire more workers and raise the salary, and pour more money ... ( Word Reader - Unregistered ) www.word-reader.com your saving So not take too many risk, instead, let those finance organization to take More over, saving money is also not good for a nation`s ... amount of money, then a lot of goods or service will have no customer, which will cause those company to cut cost, layoff workers, and cause less income for the residents in that country, which...

Ngày tải lên: 27/08/2016, 11:02

... Fluorescence emission was recorded at 460 and 580 nm After a 20 sec baseline reading, 0.1 ml of PBS containing 10 µM of the compound investigated was added, and the fluorescence signal was recorder ... than phlorizin; while thioglycoside VII had a more pronounced inhibitory effect on hSGLT1 than on hSGLT2 (p < 0.01) The inhibitory effect of thioglycoside I was stronger for hSGL2 than for hSGLT1, ... Metab Disord 2004; 5: 89-97 Klein R Hyperglycemia and microvascular and macrovascular disease in diabetes Diabetes Care 1995; 18: 258-268 Haffner SJ, and Cassells H Hyperglycemia as a cardiovascular...

Ngày tải lên: 26/10/2012, 10:04

... These examples can be corrected as follows: Although she was tired, she went to work Or She was tired, but she went to work As you know, I work very hard Or You know that I work very hard Because ... on its use for a particular purpose or object For the purpose of a discussion on error correction in foreign language teaching, this writer defines an error as an utterance, form, or structure ... AND, BUT, OR to connect sentences The expected answers of this part are or, but, or, and, or, but (or and), and, or, but (or and), and, or, and but, or, and Students make a total of 83 errors with...

Ngày tải lên: 20/12/2013, 18:16

... treatment In contrast, the 80 kDa secreted molecule was insensitive to Endo-H (B) The cell associated 70 kDa form of GPV was resistant to neuraminidase, whereas the soluble 80 kDa form was reduced to ... presence or absence of GPIb–IX and in both cases an immature cell associated 70 kDa form was detected at early chase times In cells expressing only GPV, this form was still present at later chase ... Sylvette Chasserot for help in confocal microscopy studies at Plateforme d’Imagerie In vitro IFR des Neurosciences (Strasbourg) Catherine Strassel is supported by a grant for ARMESA ´ ´ (Association...

Ngày tải lên: 23/03/2014, 13:20

... oligonucleotide solution was indicated by the fluorescence detector in all cases The measured fluorescence intensities reveal a higher rate of uptake for the smaller PTO and, for reasons unclear as yet, a reduced ... obtained are not biased to more than 20% by adsorption onto the surface of cells or culture plate was ascertained in exploratory experiments using conditions with comparable adsorption but different ... the cells was assessed by the addition of Trypan Blue Excitation was performed at 365 nm (dansyl), 488 nm (Fluos) or 543 nm (Trypan Blue) and emission was measured at 420, 515 nm or 570 nm, respectively...

Ngày tải lên: 23/03/2014, 21:21