lipids as modulators of cell functioning

... for chronic disease A nested case–control study using a UK-based general practice research database has also shown that current use of statins was associated with a reduced risk of fracture (OR ... both simvastatin [11] and pitavastatin [12] increased human osteoblast differentiation as measured by, respectively, alkaline phosphatase expression and mineralisation or expression of BMP-2 ... conceivably increase bone formation Statins produce increased proliferation and differentiation of progenitors of endothelial cells [18] In addition, atorvastatin increased the numbers of circulating...

Ngày tải lên: 09/08/2014, 03:24

... decreased dynamic compliance values for the last hour of ventilation Ventilation efficiency index decreased and oxygenation index increased in lambs with Sat PC < μmol/kg, indicating decreased gas ... decreased injury in lambs with Sat PC level > μmol/kg Cytokine mRNA levels were determined by RNase protection assays with L32 as an internal loading control Values are expressed as fold increase ... with sloughing of epithelium and inflammatory cells Injury scoring between groups was higher in low Sat PC animals (Table 2) In situ localization of HSP70 mRNA demonstrated loss of the mRNA from...

Ngày tải lên: 12/08/2014, 11:22

... Renal diseases 28 1.1.6 Inhibitors of aquaporins 1.2 MicroRNAs 31 32 1.2.1 The discovery of microRNAs 32 1.2.2 Biogenesis of miRNAs 33 1.2.3 Mode of action of miRNAs 36 1.2.4 miRNAs as endogenous ... MICRORNAS AS MODULATORS OF AQUAPORINS SUGUNAVATHI SEPRAMANIAM B.Sc (NUS), M.Sc (QUT) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOCHEMISTRY NATIONAL UNIVERSITY OF SINGAPORE ... Identification of miRNAs binding to AQP4 M1 184 promoter region 5.3 Evaluation of promoter activity of AQP4 M1 185 5.4 Effect of miRNA modulation of the promoter activity 186 of AQP4 M1 5.5 Effect of miR-130a...

Ngày tải lên: 09/09/2015, 18:52

... 26S proteasome 19S cap Ubiquitin 4315 Quinone reductase as regulator of the proteasome S Sollner and P Macheroux The impact of the 20S proteasome on QR activity raised the question of whether ... latter protein gives rise to increased levels of intracellular ROS, thereby promoting apoptosis of damaged cells [46,47] This generation of ROS increases expression of NQO1 [48] which then further ... mammalian to yeast cells, as well as regulatory proteins such as ODC, suggest that short-lived proteins are intrinsically prone to degradation by the 20S proteasome The association of a QR (NQO1,...

Ngày tải lên: 16/03/2014, 02:20

... overproduction of GPCRs in cellular expression systems based on bacterial, yeast or insect cells is still complicated and often inefficient [5–11] Continuous exchange cell- free (CECF) expression systems based ... possibility of inserting membrane proteins directly into detergent micelles upon translation The efficiency of this solubilization mode was nearly 100% in the case of ETB, as no residual precipitate was ... Ni-chelate chromatography Coelution of purified ETB with ET-1 The mode of CF expression, i.e expression as precipitate or as soluble protein, as well as the type of FEBS Journal 274 (2007) 3257–3269...

Ngày tải lên: 16/03/2014, 10:20

... hard and soft palate lesion Preoperative hard and soft palate lesion At the age of seven the patient was recovered for an orthopaedic trauma He had been diagnosed with FA at the age of seven after ... adjacent soft palatal mucosa The surface was erythematous and smooth, with some telangiectasias Clinical examination showed no regional lymphadenopathy CT and MR imaging showed a hard and soft tissue ... The patient has been followed up for months without any evidence of recurrence or metastasis The severity is determined by specific complementation group and over all by the type of genetic mutation...

Ngày tải lên: 11/08/2014, 23:22

... milk sample was classified as positive if at least one colony-forming unit (CFU) of S aureus was isolated For other agents, the presence of at least three CFU was needed for positive classification ... specificity of CMT as an indicator of IMI, for different CMT cut-offs Figure shows the sensitivity and specificity for SCC measured by DCC (SCC-DCC) as an indicator of IMI Page of Table Number of samples ... [13] was used to optimize the cut-off of the sensitivity and specificity test Results Bacteriology and somatic cell count Intramammary infection, defined as growth of udder pathogens, was found...

Ngày tải lên: 12/08/2014, 18:22

... light chain kinase and G-protein receptor lingands, such as angiotensins Modulation of the smooth muscle cell type may occur as a result of variations of conditions, including cell- cell interactions, ... hepatocytes as a result of co-culture (Takayama 2007) In spite of the many advantages of cell- sheet approaches, the technique is limited by several challenges First, the issue of vascularisation of stacked ... Engineering as a Solution to Organ Shortage Organ transplantation has become increasingly common in the past 30 years and has saved many lives, while having improved the quality of lives of thousands of...

Ngày tải lên: 14/09/2015, 08:49

... same medium, fibroblastic cells quickly grew out from fetal liver mononuclear cells, and failed to express endothelial phenotype 122 Chapter Isolation and Characterisation Of Vascular Progenitors ... Chapter Isolation and Characterisation Of Vascular Progenitors Figure 4-3: Primary culture of haemopoietic tissue in EGM Following culture in endothelial cell media, outgrowth colonies were generated ... (a) umbilical cord blood mononuclear cells with typical cobblestone morphology Similarly, outgrowth cells could be generated from (b) fetal blood mononuclear cells, which appear to have a more...

Ngày tải lên: 14/09/2015, 08:49

... clusters of FB-EPC The stained cells were also noted to adopt a more fibroblastic orphology Similarly, vWF was sporadically expressed on the cells A functional assay of acLDL uptake was performed ... transferred a portion of the cells to semi-solid cultures for haematopoietic colony forming assays as an assessment of the retention of haematopoietic character following expansion As seen in Figure ... profile was compared with FB-EPC, as well as HUVE cells (Figure 4-12) As seen in Figure 4-12, terminally differentiated EC such as HUVEC exhibit hexagonal morphology, and uniform staining of...

Ngày tải lên: 14/09/2015, 08:49

... on cell adhesion, as well as blood compatibility Finally, I studied the assembly of cells and film to form a layered structure The tunicae in vascular tissue are unique in the composition of cells: ... strength of the film following modifications No significant drop was observed as a result of plasma treatment, nor was their any trend to suggest mechanical compromise, irrespective of the number of ... repeated washing My results confirm the feasibility of the use of plasma based methods for the surface modification of µXPCL films without affecting its tensile strength 5.4.2 Critical Assessment...

Ngày tải lên: 14/09/2015, 08:49

... used to determine the amount of ATP in mg of protein measured from the number of cells per well After 24 h, the microtiter plate was taken for scintillation counting of radioactive [14C]AMG using ... validate this assay, studies with D-glucose as a substrate of SGLT were performed and the correlation of sodium-dependent D-glucose uptake to sugar-induced cell membrane depolarization, as measured ... Fluorescence emission was recorded at 460 and 580 nm After a 20 sec baseline reading, 0.1 ml of PBS containing 10 µM of the compound investigated was added, and the fluorescence signal was recorder for...

Ngày tải lên: 26/10/2012, 10:04

... them, the software outsourcing stands out as one of the most profitable and fast-growing industry This paper studies the current situation of software outsourcing development, some of the favorable ... organizations The packaged software domain includes all software sold as merchantable products In terms of the software outsourcing activities, we have other kinds of classification such as business process ... traditional reason for offshore development Low-level tasks such as coding and software testing can be performed in less-developed countries at costs as low as ten percent of domestic ones Software outsourcing...

Ngày tải lên: 27/10/2012, 16:41

... faced a historically high flood, as severe as that of 1961 which was the most destructive flood in past 70 years The flood in 2000 was extreme not only in terms of its very high peak level and ... areas are as important as safety helmets for people driving motorbikes on the highways In recent years, hundreds of children have drowned due to lack of life vests Children in the flood areas ... consideration of the activity effectiveness of structural and non-structural measures and analysis of human behaviour on flood risks in the Mekong River Delta REFERENCES Asian Disaster Reduction...

Ngày tải lên: 18/10/2013, 12:15

... Carbon-Rich Biomass Wood gas (or synthesis gas) is another form of biogas produced by thermal gasification of woody biomass or other carbon-rich materials in a gasifier or wood gas generator Usable ... switchgrass, barley, hemp, kenaf, potatoes, sweet potatoes, cassava, sunflower, fruit, molasses, corn, stover, grain, wheat, straw, cotton, biomass in general as well as many types of cellulose waste ... end of bioenergy production, i.e., improvement of cellulosic biomass /cell wall production in herbaceous grasses such as sorghum, sugarcane, and switchgrass Likewise, improvements to woody biomass...

Ngày tải lên: 25/10/2013, 05:20

... neuronal precursor cells leads to a dramatic loss of proliferative capacity of such cells (Knoepfler et al 2002) As long as questions of redundancy between different members of the myc gene family ... fibroblasts that carry a floxed allele of c-myc arrest upon cre-mediated excision of c-myc (de Alboran et al 2001) Importantly, deletion of the mnt gene, which encodes a member of the Mad family of ... the Mad family of antagonists of Myc, restores proliferation to c-myc deleted cells, suggesting that the balance of expression of members of the Myc/Max/Mad network of proteins (see Fig 1) may...

Ngày tải lên: 25/10/2013, 21:20

... during G1, was maximal just as cells entered S phase and it peaks before cyclin A-associated kinase activity (Koff et al 1992) It was absent in G1 and first detected as cells entered S phase This ... Tumors and in Tumor Cell Lines As we have described at the beginning of this chapter, the textbook model of cell cycle regulation has assigned Cdk2 the function of a master regulator of G1/S transition ... mammalian cells at the beginning of S phase to induce the initiation of DNA synthesis 2.4 G1 Phase in Mammalian Cultured Cells In the first half of the 1990s, it was shown that in mammalian cells,...

Ngày tải lên: 25/10/2013, 21:20

... more easy, than try itself to understand it Waste time - is the main problem of student who spend a lot of time and force for solving a problem, which can be solved with the help of PC faster ... of the problem of computer illiteracy is the lack of useful information in the course of information science The second cause is unwillingness to learning - many people think that easier to ask ... computer literacy is on of the main requirement at employment Thus we see that the problem of computer illiteracy is one of the main problems for business-students because of it we waste time, we lose...

Ngày tải lên: 26/10/2013, 17:15

... action in support of the final refusal were copies of a number of third-party trademark registrations on the Principal Register based on use In each registration, the list of goods includes skin ... be affirmed As applicant points out, in any likelihood of confusion analysis, two key considerations are the similarities of the goods and the similarities of the marks Tricia Guild Associates ... vitamin supplements of herbal or organic compounds,” in Class 5, that confusion would be likely In support of the refusal to register, the Examining Attorney included copies of excerpts retrieved...

Ngày tải lên: 20/12/2013, 23:15

... schoolchildren of working or studying parents, or in cases where the child has an individual need A place should be offered as close to the child’s home as possible As in the case of pre-school ... qualifying task has been complemented with its socializing task But as Isling claims, in the struggle between these two tasks the qualifying assignment has been dominant The double requirement has resulted ... that play has for learning as strongly as Fröbel had done Play June 28, 1840 was the date of the inauguration of the public German kindergarten This day was chosen by Fröbel because it was the 400-year...

Ngày tải lên: 16/01/2014, 16:33