ligands and conformational regulation of cell adhesion
Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
... modified chromosomal DNA comprises about 50% of 1,2-GG IACs, 25% of 1,2-AG IACs, 10% of 1,3-GNG IACs and interstrand crosslinks, and another 2–3% of monofunctional adducts It has been found that ... facts that modification of particular sites in the genome of living cells is naturally stochastic on the one hand, and influenced by the actual structural and functional state of the given chromatin ... the p53 protein and activate an analogous set of downstream genes Multiple splice isoforms of the p73 protein have been found that differ in the structure of their N-terminal and ⁄ or C-terminal...
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Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx
... translocation of the full-length Tat protein and cellular uptake of its basic domain make use of the same mechanism Both genetic and biological evidence indicates that the cellular uptake of the Tat ... performed on HeLa cells instead of CHO K1 cells Cells were then incubated with of lgámL)1 Tat±GFP fusion protein or with lM ¯uorescein Tat peptide and analyzed by FACS RESULTS Uptake and cellular localization ... speci®c as a large number of cell lines tested so far entrapped the translocating peptide Fig In¯uence of temperature on the uptake of Tat CPP and of Tat±GFP fusion protein HeLa cells were incubated...
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Báo cáo khoa học: Dynamin-related proteins and Pex11 proteins in peroxisome division and proliferation doc
... portals of entry into the cell Nature 422, 37–44 72 Kosaka T & Ikeda K (1983) Reversible blockage of membrane retrieval and endocytosis in the garland cell of the temperature-sensitive mutant of Drosophila ... distant relative of dynamin Loss of Dnm1 leads to the formation of a mitochondrial net throughout the yeast cell, whereas loss of Fzo leads to mitochondrial fragmentation as a result of uncompensated ... (2005) The control of peroxisome number and size during division and proliferation Curr Opin Cell Biol 17, 376–383 Lazarow PB & Fujiki Y (1985) Biogenesis of peroxisomes Annu Rev Cell Biol 1, 489–530...
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Báo cáo khoa học: DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52 pot
... effect of DNA on hydrodynamic radius (Rh) of hRad51 hRad51 (1 lM) was incubated with 0, 1, 3, and lM of oligo PUC+ (in the absence of any nucleotide cofactor) followed by the measurement of hydrodynamic ... Disassembly and recruitment of hRad51 and hRad52 proteins shorter and thicker nucleoprotein filaments when ssDNA is added [28] Tumour suppressors BRCA2 and p53 interact with the oligomerization domain of ... induced conformational changes on hRad51 protein hRad51 (25 lM) was incubated in binding buffer in the absence (lanes and 3) or presence of 75 lM ssDNA (lanes and 5) and mM ATP (lanes and 5) for...
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Báo cáo khoa học: Differential expression of liver and kidney proteins in a mouse model for primary hyperoxaluria type I pdf
... peroxisomal (FP) and cytosolic (FC) fractions Densitometry of western blot bands was performed and units of selected proteins were calculated A representative western blot band for each protein of each ... J R Hernandez-Fernaud and E Salido compared with wild-type controls by 2-DE separation and MS The analysis of specific subcellular fractions was necessary to obtain highly informative and reproducible ... bisphosphatase and catalase, whereas liver enolase and carbonic anhydrase were downregulated Comparable amounts of b-actin were present in AgxtKO and wild-type cytosolic fractions, and the absence of AGT1...
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Báo cáo khoa học: Biogenesis of peroxisomes Topogenesis of the peroxisomal membrane and matrix proteins ppt
... standard secretion pathway One of these novel import pathways into the ER is the topogenesis of Ist2p The import of Ist2p is mRNAdependent and takes place at the cortical ER of the daughter cell ... protein Pex18p and the N-terminal half of the PTS1 receptor Pex5p Mol Cell Biol 24, 8895–8906 Matsumura T, Otera H & Fujiki Y (2000) Disruption of the interaction of the longer isoform of Pex5p, Pex5pL, ... and to require the N-terminus of the receptor [62,63] The current understanding of the organization of the peroxisomal import machinery for PTS1 proteins is summarized in Fig In the absence of...
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applications of chimeric genes and hybrid proteins, part a
... purposes, especially the analysis of membrane proteins and intracellular protein trafficking, and for monitoring and manipulating various aspects of cell signaling and cell physiology Included in this ... strains Pellet the cells from a fresh 5-ml overnight culture of MC4100 and resuspend in 2.5 ml of 10 m M MgSO4 In a small culture tube, mix 50/zl of cells with 10/zl of each dilution of the h lysates ... tube of cells and phage add 2.5 ml of prewarmed (37 °) LB broth containing 40 ~1 of freshly added X-Gal (20 mg m1-1) stock solution per ml of LB broth Add 2.5 ml of molten (45 °) LB top agar and...
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applications of chimeric genes and hybrid proteins, part b
... distribution of cognate ligands or receptors in tissues or cells In [15] in this volume4 we describe other applications: molecular characterization of ligands and receptors, and the cloning of novel ligands ... Staining of Cells, Tissues, and Embryos By JOHN G FLANAGAN, MITSUHARU HATTORI, HWAI-JONG CHENG, DAVID A FELDHEIM, QIANG Lu, and PIERRE VANDERHAEGHEN Introduction Polypeptide ligands and their cell ... purposes, especially the analysis of membrane proteins and intracellular protein trafficking, and for monitoring and manipulating various aspects of cell signaling and cell physiology Included in this...
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báo cáo hóa học:" Proteomic characterization of HIV-modulated membrane receptors, kinases and signaling proteins involved in novel angiogenic pathways" ppt
... diverse cellular functions, including cell- cell interactions, cell adhesion, cell aggregation, cell migration, cell cycle progression, differentiation, inflammation, angiogenesis, and maintenance of ... cells [163,164] Expression of this enzyme is vital for phosphorylation of cellular proteins involved in contraction of cells, regulation of cell shape and formation of new structures such as gap ... Thus, the overexpression of ERBB-PTK, GRB2, ZAP-70, MAPK, dysregulation of integrins and upregulation of adhesion kinase, all contribute to the formation of vasculature and promote angiogenesis...
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báo cáo hóa học:" Correlation between expression of p53, p21/WAF1, and MDM2 proteins and their prognostic significance in primary hepatocellular carcinoma" docx
... expression of p53, p21/WAF1, and MDM2 in the 181 pairs of tissues was analyzed by IHC As shown in Figure 2, p53, p21/WAF1, and MDM2 were mainly located in the nuclei of the cancer cells and highly ... Figure Expression of p53, p21/WAF1 and MDM2 in HCC by IHC Expression of p53, p21/WAF1 and MDM2 in HCC by IHC The hematoxylin and eosin (H&E) stained sections show a solid area of hepatocellular carcinoma ... p21/WAF1, and MDM2 in 181 pairs of HCC on the basis of different clinical parameters, including the gender, age, tumor size, number of tumors, HBsAg, pathologic grade, and serum level of AFP of the...
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báo cáo hóa học: " Leader (L) and L* proteins of Theiler''''s murine encephalomyelitis virus (TMEV) and their regulation of the virus'''' biological activities" docx
... [30,31], and the second is cleavage of the p220 component of the cap-binding protein complex [32], resulting in the shut off of host protein synthesis On the other hand, the function of L of the ... that is, inflammatory cell infiltration and loss of myelin in the face of relative preservation of axons [1-4] Therefore, this demyelinating disease is considered to be an excellent animal model ... iron/ferritinmediated activation of NFκB [34] The functions of L of Page of (page number not for citation purposes) Journal of Neuroinflammation 2006, 3:19 TMEV and the other cardioviruses remain...
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Báo cáo hóa học: " Respiratory syncytial virus (RSV) attachment and nonstructural proteins modify the type I interferon response associated with suppressor of cytokine signaling (SOCS) proteins and IFN-stimulated gene-15 (ISG15)" potx
... understand aspects of the immunobiology of infection Mouse lung epithelial (MLE)-15 cells offer a good option to emulate the mouse model of RSV infection as these cells are a type II pneumocyte cell ... infection of MLE-15 cells and IFNα, IFNβ and SOCS1 and SOCS3 mRNA expression was examined at 48 h pi At 48 h pi, ΔNS1/2 virus infected MLE-15 cells had significantly (p < 0.01) higher IFNα and IFNβ ... prised of at least 14 IFNα genes and IFNα pseudogenes [57], and because the quantity of IFNα measured depends on the specificity of the detection antibody for these isoforms, detection of IFNα...
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Báo cáo y học: "Diagnostic value and clinical laboratory associations of antibodies against recombinant ribosomal P0, P1 and P2 proteins and their native heterocomplex in a Caucasian cohort with systemic lupus erythematosus" pot
... and 11 (7.8%) positive for aRibPR2 Some of those sera were further exclusively positive for one of the recombinant aRibPs and showed an increased titer up to twofold of the corresponding cutoff ... ethnicity of the study cohort and differences of the test systems Cases of neuropsychiatric lupus [28,29] and subtypes of lupus nephritis were not recorded in our study The most striking association of ... of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis RB, CD, FH and WSchlumberger contributed to study design FB, RB, US, KE and...
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Báo cáo y học: "Rev and Rex proteins of human complex retroviruses function with the MMTV Rem-responsive element" doc
... function in human cells The reports of MMTV infection of human cells and detection of MMTV sequences in breast cancers and lymphomas [14-18] appear to be feasible since most steps of viral replication ... RmRE in human and mouse cells A Reporter activity in Jurkat T cells B Western blotting of Rem and Rec expression in Jurkat cells Results using antibodies specific for GFP (upper panel) and actin ... Western blots of extracts from Rex and Remtransfected HC11 cells A GFP-related band is observed in this blot (asterisk; lanes and 5), but the major band is not visible in this portion of the gel...
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Báo cáo y học: " Determination of the relative amounts of Gag and Pol proteins in foamy virus particles" doc
... series of recombinant Gag(IN) Pol proteins, a cellular lysate (C), and extra-cellular virus (V) detected Immunoblot of SGG1 (Gag), 15E10 (RT), and 3E11 and Immunoblot of a dilution series of recombinant ... Figure of loaded onto the gel Relation4 the intensities of the bands in the lanes with recombinant PFV proteins shown in Fig and amounts of protein Relation of the intensities of the bands in ... amounts of bacterially-expressed Gag and Pol proteins were also applied to the gel The intensities of the bands were determined with a LAS-3000 (Fujifilm) and the relative amounts of Gag and Pol...
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Báo cáo y học: "Phylogenetic and structural analysis of centromeric DNA and kinetochore proteins" doc
... inof setidenClickresiduessuccessiveof ofofresidues,residues proteinsWhiteprofile 1 .and metazoanS.ofofin numbers multipleusedininthisMtw1,subunit the organisms Accession proteinsblocksfungi andandcuniculi.resiorganismsoflettersusedall ... Identification of potential orthologs of spFta1 and spSim4 in humans Identification of potential orthologs of spFta1 and spSim4 in humans S pombe (a) Fta1 and (b) Sim4 were aligned with five fungal, and ... in plants and metazoan NR and EST database Metazoan/plant orthologs Multiple sequence alignment of metazoan/plant proteins Clustal-W and T-Coffee deposited research Clustal-W and T-Coffee Correspondence...
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Studies on interactions between HCRSV coat protein and host proteins from kenaf
... Co-localization of HCRSV CP and HcSO using BiFC 80 4.2.3 Protein expression and pull down assay of CP with HcSO 81 4.2.4 Determination of HcSO transcript level and sulfate level in mock and HCRSV ... experiment of RPL10A study 115 Table 5.2 Interaction between HCRSV CP and L10A and identification of domains of CP that are involved in the interaction in yeast cells 130 x LIST OF FIGURES ... co-localization of HCRSV CP and HcSO in kenaf protoplasts 87 Figure 4.2 Subcellular localization of HCRSV CP and HcSO in kenaf epidermal cells (a to c) and colocalization of the two proteins to peroxisomes...
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X ray crystallographic study of yeast dcp1 and dcp2 proteins insights into the mechanism and regulation of eukaryotic mRNA decapping
... first year of rotation, Dr Qi Xie and Dr Mohan Balasubramanian at the ex-Institute of Molecular Agrobiology, for their guidance and patience I acknowledge the Institute of Molecular and Cell Biology ... patch consists of several aromatic residues forms the PRS binding site The PRS ligands adopt a left-handed PPII helix and the pyrrolidine rings of the proline residues on one edge of the PPII prism ... Activity of mutants in the conserved patch of scDcp1p 70 Figure 15 Activity of mutants in the conserved patch of scDcp1p 72 Figure 16 Activity of mutants in the hydrophobic surface patch of scDcp1p...
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Crystallization trials of refolded breast tumor kinase (BRK) and peroxisome proteins
... presence of transmembrane and extracellular domains, which enable PTKs possessing them to recognize extracellular ligands, in particular, various peptide growth factors Specific ligands and intracellular ... variety of multigene family evolved to perform functions that regulate a range of cellular processes, including cell growth, differentiation, apotosis, motility, adhesion, and cell- to -cell communication ... primitive unit -cell and the bigger unit -cell in some selected crystal systems is called a centered unit -cell The geometry of a unit -cell is defined by three non-coplanar axes (a, b, c) and their inter-axial...
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Crystallization trials of refolded breast tumor kinase (BRK) and peroxisome proteins 1
... proportion of cutaneous T -cell lymphomas and other transformed T- and B -cell populations Brk also has conferred in vivo oncogenicity on the BaF3 cells Furthermore siRNA-mediated inhibition of endogenous ... CRYSTALLOGRAPHY 1.3.1 1.3 Unit cell and lattices 1.3.2 Symmetry, point group and space group 1.3.3 Crystals and X-rays 1.3.4 X-ray diffraction 1.3.5 Bragg’s law 1.3.6 Reciprocal lattice and Ewald sphere 1.3.6.1 ... 16 2.1.4 Role of Brk in lymphoma 20 THE IMPORTANT ROLES OF PEX8 AND PEX20 IN PICHIA PASTORIS 23 2.2.1 Peroxisome biogenesis and degradation 23 2.2.1.1 Peroxisomal constituents and its functions...
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