levels via mitosox red staining

Human telomerase reverse transcriptase (hTERT) overexpression modulates intracellular redox balance and protects cancer cells from apoptotic cell death

Human telomerase reverse transcriptase (hTERT) overexpression modulates intracellular redox balance and protects cancer cells from apoptotic cell death

... …………………………………………………….58 13 Assessment of intracellular ROS levels via CM-H2DCFDA staining …… 58 14 Assessment of mitochondrial O2.- levels via MitoSOX Red staining ………58 15 Assessment of mitochondrial membrane ... catalytically reduced by thioredoxins Hyperoxidised Prxs are reduced by sulfiredoxins (Rhee et al., 2005a) 4.5 Thioredoxins The mammalian thioredoxins (Trxs) are a family of small (approximately 12 kDa) redox ... Thioredoxin reductase (TrxR) reduces the active site disulfide in thioredoxins (Trx) and several other substrates directly under consumption of NADPH Reduced thioredoxin is highly efficient in reducing...

Ngày tải lên: 14/09/2015, 08:38

200 686 0
Nile red staining for lipids

Nile red staining for lipids

Ngày tải lên: 09/05/2016, 16:56

1 73 0
Tài liệu Báo cáo khoa học: Treatment of neutral glycosphingolipid lysosomal storage diseases via inhibition of the ABC drug transporter, MDR1 Cyclosporin A can lower serum and liver globotriaosyl ceramide levels in the Fabry mouse model doc

Tài liệu Báo cáo khoa học: Treatment of neutral glycosphingolipid lysosomal storage diseases via inhibition of the ABC drug transporter, MDR1 Cyclosporin A can lower serum and liver globotriaosyl ceramide levels in the Fabry mouse model doc

... mice had serum Gb3 levels of 3.31 ± 1.33 ngÆlL)1 compared with control Fabry serum levels of 8.21 ± 2.27 ngÆlL)1 as determined from a standard curve Serum Gb3 levels were monitored in all animals ... accumulates) [2–4] a-Galactosidase administered to Fabry patients is able to reduce serum levels of Gb3 by 50% [5], liver Gb3 and by inference, kidney Gb3 levels [5,6] In the Fabry mouse model, in ... in (a) and (b) show Kupffer cell staining, and in (d) portal vein endothelial VT1 cell staining Most VT1 staining is lost after CsA treatment but portal triad staining was retained 2070 FEBS Journal...

Ngày tải lên: 19/02/2014, 07:20

12 432 0
LINUX RED HAT

LINUX RED HAT

... #/etc/pam.d/other auth required /lib/security/$ISA/pam_deny.so account required /lib/security/$ISA/pam_deny.so password required /lib/security/$ISA/pam_deny.so session required /lib/security/$ISA/pam_deny.so ... phiên RedHat 7.x chạy telnetd không sử dụng inetd (mà sử dụng xinetd - phiên nâng cấp có nhiều cải tiến so với inetd) nên cách cấu hình lại telnetd khác tuỳ theo phiên RedHat sử dụng + Với phiên RedHat ... #%PAM-1.0 auth required /lib/security/pam_securetty.so auth sufficient /lib/security/pam_rhosts_auth.so auth required /lib/security/pam_stack.so service=system-auth auth required /lib/security/pam_nologin.so...

Ngày tải lên: 18/08/2012, 11:19

32 957 3
SỬ DỤNG CÂN CHỈNH HIỆU ỨNG LEVELS

SỬ DỤNG CÂN CHỈNH HIỆU ỨNG LEVELS

... Level GIMP (trái) Photoshop (phải), trông tương tự Cả hai có hai trượt bản, Input Levels lại Output Levels 11 Input levels dùng để điều chỉnh vùng giá trị cách thay đổi ba điểm trục ngang Di chuyển ... ban đầu Nhấp Phím Ctrl+L để mở Levels Dùng Black Point để chấm điểm tối White Point để chấm điểm sáng Nếu bạn chọn không dúng vị trí ảnh bị chát Bạn rê trượt Output Levels để cân màu sắc Trong lc1 ... Mục Channel: Ngoài màu RGB có màu Red – Green – Blue Khi bạn chọn màu, bạn rê xiêng lên quan sát ảnh thấy tô đậm màu chọn Nếu rê xuống thấy màu khác xuất  Chọn Red: Khi nâng lên ảnh có màu đỏ...

Ngày tải lên: 27/08/2012, 11:47

14 693 4
Characterization and deactivation of sulfided red mud used as hydrogenation catalyst

Characterization and deactivation of sulfided red mud used as hydrogenation catalyst

... that fresh red mud is made up of particles partially covered by small granules After 12 h run, besides zones similar in appearance to the fresh red mud (Fig 4a), other zones appeared formed by ... flat-surfaced particles, which were less covered by granules than fresh red mud (Fig 4c and d) Table gives the concentration of the different red mud samples measured by SEM-EDX As the reaction proceeded, ... EDX composition (wt-.%) Fe Ti AI Na Ca Si 19.7 13.0 7.9 3.7 5.1 4.7 P V C1 not measured not measured not measured 21.7 11.9 7.4 3.0 4.9 3.6 0.7 0.3 0.3 Catalyst morphology was studied by SEM in...

Ngày tải lên: 23/09/2012, 14:46

15 509 0
Removal of congo red from wastewater by adsorption onto waste red mud

Removal of congo red from wastewater by adsorption onto waste red mud

... was to evaluate red mud for the treatment red, waste red mud for the treatment Zouboulis for the removal The objective feasibility and solid (10) and for the removal aqueous red mud industrial ... Coloured waters are often objectionable on aesthetic grounds for drinking and other agricultural purposes Colour affects the nature of the water by inhibiting sunlight penetration thus reducing ... adsorbent water for red Tamil powder was from was obtained M/S India) Nadu, was washed particulate fine The obtained thoroughly was very h Congo studies mud (Mettur, It to remove 60°C red used used...

Ngày tải lên: 23/09/2012, 14:47

17 897 1
Heavy metal cation retention by unconventional sorbents (red muds and fly ashes)

Heavy metal cation retention by unconventional sorbents (red muds and fly ashes)

... metal/sorbentcombinationswere as follows: Cu (II) 50 mM (mmol/litre)for red muds and 90 mM for fly ashes; Pb (If) 50 mM for red muds and 65 mM for fly ashes; Cd (II) 35 ram for red muds and 40 mM for fly ashes, bCalculatedby ... ahiminosilicates 94% of red muds have less than 10/an grain size The red muds were thoroughly washed with water to a neutral pH, dried and sieved (R,) prior to adsorption tests The red muds were also ... fly ashes and red muds in the activation of red mud) was avoided due to severe losses of fly ash components by solubilization The solid product was thoroughly washed with water, filtered, and oven...

Ngày tải lên: 23/09/2012, 14:47

11 514 0
Phosphate removal from aqueous solutions using red mud wasted in bauxite Bayer's process

Phosphate removal from aqueous solutions using red mud wasted in bauxite Bayer's process

... Dependencebetween the mixing time of red mud and OPh-solution and PO3 removal(•-raw red mud, ~-activated red mud) (30 rain for raw mud and 15 for activated red mud) The same tendency was more ... investigated the properties of a red mud as a coagulant and the physiological effects of its use as well [3-5] Weaver and Ritchie have compared lime-based materials and red mud for phosphorus removal ... Changing the red mud dose in a range of 40 g/l has shown that 30 g/l is sufficient for the complete removal of PO 3- from TPPh-solution At the same time a dose of 40 g/1 red mud was required to give...

Ngày tải lên: 23/09/2012, 14:47

10 661 0
 Báo cáo khoa học: "What do we know about medication errors made via a CPOE system versus those made via handwritten orders"

Báo cáo khoa học: "What do we know about medication errors made via a CPOE system versus those made via handwritten orders"

... terra incognita This research reminds us that while CPOEs undoubtedly reduce several forms of medication error, measuring such reductions requires us to address the multifaceted reality of error ... certainty, error severity and, indeed, the ability to determine that an error occurred Moreover, because error reduction is far from the only benefit we anticipate from CPOEs (e.g., they also confer ... cause injury.” Thus, we must consider that among CPOE systems’ many virtues is their ability to reduce errors that seldom reach patients (which neither negates their many valuable contributions...

Ngày tải lên: 25/10/2012, 10:39

2 524 1
Báo cáo y học: "Trace Elements, Heavy Metals and Vitamin Levels in Patients with Coronary Artery Diseas"

Báo cáo y học: "Trace Elements, Heavy Metals and Vitamin Levels in Patients with Coronary Artery Diseas"

... tocopherol levels were lower in CAD patients compared to controls As far as we know, there is no study examining serum Cd levels in CAD in the literature We have found that serum Cd levels were ... decreased retinol levels in patients with CAD (31,32) In other studies, Levels of retinol were similar in patients with CAD compared to controls (5,6,31,32) Numerous studies have explored whether retinol ... considered significant RESULTS AND DISCUSSION Clinical characteristics and serum cholesterol levels were not significantly different in patients with and without CAD (Table 1) Comparisons of the levels...

Ngày tải lên: 25/10/2012, 10:51

5 527 0
 Báo cáo y học: "he Association Among Lipoprotein-associated Phospholipase A2 Levels, Total Antioxidant Capacity and Arousal in Male Patients with OSA"

Báo cáo y học: "he Association Among Lipoprotein-associated Phospholipase A2 Levels, Total Antioxidant Capacity and Arousal in Male Patients with OSA"

... measured using both a nasal cannula (NC) and nasal thermistor, oxygen saturation (SaO2) was measured using a pulse oximeter, and chest and abdominal respiratory movements were monitored A reduction ... markers and arousal in patients with OSA Methods Patients who were referred to the Sleep laboratory for evaluation of sleep-disordered breathing between April 2009 and January 2010 were prospectively ... cardiovascular disease) enables a more accurate prediction of cardiovascular risk to be made, as there are clearly established relationships between levels of various circulating haemostatic risk...

Ngày tải lên: 25/10/2012, 11:00

8 508 0
Báo cáo y học: " Laugh Yourself into a Healthier Person: A Cross Cultural Analysis of the Effects of Varying Levels of Laughter on Health"

Báo cáo y học: " Laugh Yourself into a Healthier Person: A Cross Cultural Analysis of the Effects of Varying Levels of Laughter on Health"

... found to exist in MISS as compared to AUR (Table 4) According to Figure 1, emotional well-being scores significantly differed between samples (Z=-2.619, p=

Ngày tải lên: 26/10/2012, 09:57

12 758 0
 Báo cáo y học: "Relationships between free radical levels during carotid endarterectomy and markers of arteriosclerotic disease"

Báo cáo y học: "Relationships between free radical levels during carotid endarterectomy and markers of arteriosclerotic disease"

... Abbreviations: PL, I and RP OXANO levels at baseline, clamp and reperfusion respectively MCP11, MCP15, MCP19; MCP levels before, during and after clamping, ICAM11, ICAM15, ICAM19; ICAM levels ... MCP-1, ICAM-1 and MMP-9 were measured using commercially available ELISA kits from R&D systems, UK OxLDL concentration in plasma that had been stored at –70ºC was measured by a sandwich ELISA (Mercodia, ... stump pressure was measured using an arterial pressure monitor via a needle inserted into the internal carotid artery distal to the stenotic lesion A shunt was not considered necessary in any case...

Ngày tải lên: 26/10/2012, 10:03

7 640 0
Báo cáo y học: "Serum cystatin C levels to predict serum concentration of digoxin in Japanese patients

Báo cáo y học: "Serum cystatin C levels to predict serum concentration of digoxin in Japanese patients

... increase was more predominant for Cys-C than Cr Figure showed the relationships between the serum levels of Cys-C and Cr in the healthy elderly subjects and patients The serum levels of Cys-C were ... the patients showed higher serum levels of Cys-C and Cr, when compared with the healthy elderly subjects (Table 1, Fig 1) The increase in the serum level was more predominant for Cys-C (Table 1, ... coadministered medications In summary, the usefulness of Cys-C was compared with Cr in terms of the estimation of the steady-state serum trough concentrations of digoxin in Japanese patients The serum levels...

Ngày tải lên: 31/10/2012, 17:08

5 524 0
Báo cáo y học: "Cell Cycle Arrest by a Natural Product via G2/M Checkpoint"

Báo cáo y học: "Cell Cycle Arrest by a Natural Product via G2/M Checkpoint"

... cold PBS and x 106 cells were resuspended in 500 ml X binding buffer A hundred microliter of cell suspension was transferred to a ml culture tube and incubated with 10 µl of Annexin V antibodies ... bromophenol blue) were denatured at 95 °C for Total cellular proteins (20-100 µg) were subjected to SDS-polyacrylamide gel electrophoresis (PAGE) and the proteins were transferred to Hybond-P PVDF membrane ... Annexin V staining and a dose-dependent relationship was found (Fig 4) After hr of 0, 5, 10 or 15 % CKBM incubation, the apoptotic population increased from 2.8 % in the control group compared to...

Ngày tải lên: 02/11/2012, 11:12

6 319 0
Báo cáo y học: "Comparative study of serum Na+ and K+ levels in senile cataract patients and normal individuals"

Báo cáo y học: "Comparative study of serum Na+ and K+ levels in senile cataract patients and normal individuals"

... levels were measured with flam photometry method by the aid of SEAC model FLP20, Italy Outcoming data was assessed by spss 11 software and mean of aboveelectrolytes in both groups were compared ... certain diets is suggested In this order, results can be compared with similar studies Conflict of interest The authors have declared that no conflict of interest exists Int J Med Sci 2004 1(3): ... its etiology is a necessity, so that many future disabilities can be prevented, surgical costs reduced and quality of life improved Multiple mechanisms such as osmotic graduation [11,12], protein...

Ngày tải lên: 03/11/2012, 09:49

5 611 1
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