lesson from in vivo studies in non human primates
Báo cáo y học: " Expanding whole exome resequencing into non-human primates" pot
Ngày tải lên :
09/08/2014, 23:20
... polymorphism in rhesus macaques that can be used to refine their use in translational studies and to approach non- human primate genetic modeling of human disease in a unique fashion Finally, it begins ... polymorphism in non- human primates have remained focused on specific candidate genes Our ability to incorporate genetic information into our animal studies is not at issue; rather the limiting factor ... whole exome resequencing technologies can successfully be used to identify codingregion variation in non- human primates extending into old world monkeys In addition to identifying variation, whole...
- 24
- 54
- 0
Báo cáo y học: "The role of the Notch pathway in healthy and osteoarthritic articular cartilage: from experimental models to ex vivo studies" pdf
Ngày tải lên :
12/08/2014, 15:22
... ligand-receptor interaction and a cysteine rich region The intracellular domain consists of ankyrin repeats, a glutamine-rich domain and a PEST (proline, glutamate, serine, threonine) domain [14,15] ... protein implicated in cell growth and fate guidance [28-33] and in the etiology of human cancer [34-36] Interestingly, it has also been reported that mutations affecting Abl signaling result in ... also in proliferation and apoptosis in developing and mature articular cartilage, recent studies have focused on the involvement of this pathway in joint pathology Several studies were interested...
- 8
- 275
- 0
Accounting for Changes in Social Support Among Married Older Adults: Insights From the MacArthur Studies of Successful Aging pptx
Ngày tải lên :
06/03/2014, 15:20
... asked to indicate how distressed they were by feelings of hopelessness, lack of interest, worrying, feeling blue, feeling lonely, blaming themselves, feeling trapped, crying easily, having a poor ... report increases in emotional support from friends and family and more instrumental support both from friends and family and from their children, presumably because they had worked to maintain their ... support and incidence and course of chronic disease in the elderly Family Systems Medicine, 13, 39 – 68 Lang, F R (2000) Endings and continuity of social relationships: Maximizing intrinsic benefits...
- 10
- 449
- 0
Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
Ngày tải lên :
07/03/2014, 21:20
... antibodies directed against PSI-G, PSI-K (intrinsic membrane protein, stromal loop), PSI-O (intrinsic membrane protein, luminal loop) and PSI-D (extrinsic membrane protein, stromal side) Each lane corresponds ... more efficiently in the context of the PSI-G loop region than at the C-terminus Topology of PSI-G in vivo In order to examine the topology of PSI-G in vivo, samples of thylakoids from wild-type ... successfully incorporated His- and Streptagged PSI-G into Arabidopsis PSI complexes in vivo In achieving this, we have been able to collect multiple lines of evidence that PSI-G inserts into the...
- 9
- 422
- 0
Báo cáo khoa học: In vivo studies of altered expression patterns of p53 and proliferative control genes in chronic vitamin A deficiency and hypervitaminosis pot
Ngày tải lên :
08/03/2014, 08:20
... of p53 protein was significantly increased in the liver and lung from vitamin A-deficient rats that in controls c-Jun protein was significantly decreased in vitamin A-deficient rats In the liver ... controls, while in acute hypervitaminosis the binding was significantly higher These changes in the RAR–DNA binding is not due to different levels of the retinoic acid receptor induced by the treatment, ... specific binding was determined by the relative intensity of ethidium bromide fluorescence when compared to the input control Our data show that the RAR binding was almost undetectable in vitamin A-deficiency...
- 9
- 508
- 0
Clinic-Based Family Planning and Reproductive Health Services in Africa: Findings from Situation Analysis Studies pptx
Ngày tải lên :
14/03/2014, 14:20
... substantial funding for such studies in every region Throughout Asia and Latin America, the experimental findings from numerous OR studies were instrumental in helping family planning programs identify ... planning programs in India in the 1950s and in much of the rest of Asia and Latin America in the 1960s Further, policy makers tended to shy away from family planning, which was controversial in ... International Training in Health International Planned Parenthood Federation intrauterine device Johns Hopkins Program for International Education in Reproductive Health Johns Hopkins University/Population...
- 266
- 384
- 0
Báo cáo hóa học: " Non-monotonic changes in clonogenic cell survival induced by disulphonated aluminum phthalocyanine photodynamic treatment in a human glioma cell line" pot
Ngày tải lên :
18/06/2014, 16:20
... Uptake kinetics of AlPcS2 following incubation of cells with AlPcS2 in HBSS for different time intervals showed rapid and linear increase in the accumulation of AlPcS2 in the first h, prolonged incubation ... to gain insight into the mechanisms underlying these effects Materials and methods 2.1 Tumor cell lines Human cerebral glioma cell line (BMG-1; DNA index = 0.95; wild-type p53), established from ... al., Non- monotonic changes in clonogenic cell survival induced by disulphonated aluminum phthalocyanine photodynamic treatment in a human glioma cell line Journal of Translational Medicine 2010,...
- 14
- 521
- 0
báo cáo hóa học:" Functional significance of the signal transduction pathways Akt and Erk in ovarian follicles: in vitro and in vivo studies in cattle and sheep" ppt
Ngày tải lên :
20/06/2014, 07:20
... important in mediating the effects of FSH on inhibin-A, activin-A, oestradiol and progesterone secretion and also important in mediating IGF-I stimulated inhibin-A, activin-A, follistatin, oestradiol ... immunization of sheep against an amino terminal peptide of the inhibin αC subunit on intrafollicular levels of activin A, inhibin A and follistatin Journal of Endocrinology 1998, 157:157-168 ... 67:1003-1012 Jenkins PJ, Bustin SA: Evidence for a link between IGF-I and cancer European Journal of Endocrinology 2006, 151:S17-22 Evans ACO, Martin F: Kinase pathways in dominant and subordinate ovarian...
- 13
- 438
- 0
Báo cáo hóa học: " Nano-regime Length Scales Extracted from the First Sharp Diffraction Peak in Non-crystalline SiO2 and " docx
Ngày tải lên :
22/06/2014, 00:20
... modeling addressing the microscopic nature of bonding arrangements in the MRO regime including rings of bonded atoms [9], distances between layer-like ordering [10], and/or void clustering that ... symmetric rings by less symmetric rings of bonded atoms; i.e., five- and seven-member rings in SiO2 The inter-ring coupling in SiO2 is direct result of the softness of the Si–O–Si bonding force ... on internal grain boundaries of nano-grains large enough to display J–T term splittings [28] These are indicated in Fig Nano-grain HfO2 in the MRO size regime of 1.5–2 nm can also formed in phase-separated...
- 9
- 303
- 0
Probiotic Lactobacillus Strains: in vitro and in vivo Studies pot
Ngày tải lên :
07/07/2014, 19:20
... immunomodulating properties in in vivo mouse model MATERIAL AND METHODS Survival of Lactobacillus strains at low pH and in the presence of bile salt Lactobacillus strains isolated from feces of healthy infants ... 2000) Experimental design in vivo Eight-week-old inbred BALB/c mice (n = per group) were used for in vivo experiments Strains selected in in vitro tests were mixed in equal proportions, lyophilized ... compared with individual strains (unpublished results); the mixture of bacteria was, therefore, used for in vivo experiment in mice During studies in mouse model the selected strains were safe...
- 5
- 397
- 2
Báo cáo khoa học: "Human vascular adhesion proteın-1 (VAP-1): Serum levels for hepatocellular carcinoma in non-alcoholic and alcoholic fatty liver disease" pdf
Ngày tải lên :
09/08/2014, 03:22
... reagent (Boehringer Mannheim, Germany) according to the manufacturer’s instructions The intensities of the chemiluminescence reactions in the wells were always measured after a 3-min incubation ... added into the wells, and the plates were allowed to incubate at room temperature again for h Finally, plates were washed six times with Tween/PBS and generated after combining with chemiluminescence ... molecule mediating lymphocyte binding in humans Science 1992, 257:1407-9 Salmi M, Tohka S, Berg EL, Butcher EC, Jalkanen S: VAP-1 mediates lymphocyte subtype-specific, selectin-independent recognition...
- 4
- 294
- 0
báo cáo khoa học: "Detection of DNA mismatch repair proteins in fresh human blood lymphocytes - towards a novel method for hereditary non-polyposis colorectal cancer (Lynch syndrome) screening" doc
Ngày tải lên :
10/08/2014, 10:21
... approval and signed informed consent, venous blood was collected from patients using EDTA-containing vacutainer tubes Samples were collected from individuals undergoing genetic counseling for hereditary ... PMSF, 2-mercaptoethanol, PHA, penicillin, and streptomycin were from Sigma-Aldrich Lymphoprep was from Axis-Shield Human IL-2 was a gift from Dr Martin Cannon, University of Arkansas for Medical ... (Table 1) using western blotting to detect full-length MLH1 and MSH2 proteins in cell lysates from established colorectal carcinoma cell lines The results for polyclonal antibodies were inconsistent...
- 7
- 334
- 0
báo cáo khoa học: "Updates in non-small cell lung cancer - insights from the 2009 45th annual meeting of the American Society of Clinical Oncology" potx
Ngày tải lên :
10/08/2014, 22:21
... presented in 2008 ASCO annual meeting, also tested erlotinib with platinum/gemcitabine chemotherapy with erlotinib continuing as the maintenance therapy [19] PFS was statistically better in experimental ... The important finding in this trial has demonstrated the improvement of PFS and RR from vandetanib translated into the clinically meaningful delay in symptom progression Competing interests The ... al.: Phase III Study of Erlotinib in Combination With Cisplatin and Gemcitabine in Advanced Non- Small-Cell Lung Cancer: The Tarceva Lung Cancer Investigation Trial J Clin Oncol 2007, 25(12):1545-1552...
- 11
- 406
- 0
Báo cáo y học: " Human parvovirus PARV4 DNA in tissues from adult individuals: a comparison with human parvovirus B19 (B19V) pot
Ngày tải lên :
12/08/2014, 01:22
... one individual PARV4 DNA and in individuals B19V DNA were demonstrated in myocardium samples only In one individual PARV4 DNA and in individuals B19V DNA were present in all the tissues examined ... 46% and 65% in HIV infected patients [12,16] and is 7,7% in the skin of HIVnegative individuals [14] The frequency of PARV4 DNA in the liver reported in literature [13] was lower (15%) in comparison ... results are fairly in agreement with the literature where the frequency of PARV4 DNA in bone marrow varies from 0% (0/8) in HIV uninfected individuals (like the patients enrolled in this study),...
- 5
- 242
- 0
Báo cáo y học: "Distinct host cell proteins incorporated by SIV replicating in CD4+ T Cells from natural disease resistant versus non-natural disease susceptible hosts" docx
Ngày tải lên :
13/08/2014, 01:20
... the mitogen protein kinase kinase kinase kinase protein involved in cell signaling The fourth protein identified was TRIM45, which is part of the Tripartitie motif-containing proteins and is thought ... by increasing the expression of the pore forming tight junction molecule termed claudin-2 [53] These findings are of interest in light of the findings of HIV-1 induced dysregulation of claudin-2 ... activated protein kinase signaling pathway [41] Thus, these proteins appear to be either involved in ubiquitination or intracellular signaling, with one of them shown to play an important role in HIV-1...
- 15
- 220
- 0
Báo cáo y học: "Brain dysfunction in sepsis: what can we learn from cerebral perfusion studies" pdf
Ngày tải lên :
13/08/2014, 08:21
... could inform on the extent of the effect of inflammatory mediators on the brain Investigations addressing SAD will remain difficult due to the problem of correctly identifying and quantifying this ... this entity The value of monitoring cerebral perfusion and cerebrovascular reactivity in this setting remains to be defined However, further experimental and clinical investigations on SAD are important ... the age of the included patients, and in my opinion it is impossible to define a ‘normal’ CBF range for such a group of patients Moreover, ischaemic changes in relatively small brain regions could...
- 2
- 280
- 0
Báo cáo y học: "Arctigenin from Arctium lappa inhibits interleukin-2 and interferon gene expression in primary human T lymphocytes" docx
Ngày tải lên :
13/08/2014, 14:20
... identified in A lappa such as anti-bacterial infection, scavenging free radicals [18], binding platelet-activating factors [19] and inhibiting acute ear swelling [20] This study showed that AC from ... this article as: Tsai et al.: Arctigenin from Arctium lappa inhibits interleukin-2 and interferon gene expression in primary human T lymphocytes Chinese Medicine 2011 6:12 Submit your next manuscript ... phosphatase calcineurin binds to NF-AT, dephosphorylates NF-AT and causes nuclear import of NF-AT The binding domain of NF-AT is Rel similarity domain located in numerous cytokine promoters IL-2...
- 8
- 176
- 0
Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
Ngày tải lên :
13/08/2014, 23:20
... Determination of Death Act [15] In the meantime, most major IVF clinics already obtain written informed consent from patients to discard any human embryos deemed non- viable or dead Interestingly, ... approaches, in a manner to facilitate human ESC research and to promote respect for human embryos obtained from clinical reproductive endocrinology practice http://www.tbiomed.com/content/2/1/25 Human ... developed that offered human ESCs whilst preserving (or at least not destroying) the blastocysts from which they originated In this paper we present results from pilot studies based on some theoretical...
- 8
- 368
- 0
Varicella-Zoster Virus Epithelial Keratitis in Herpes Zoster Ophthalmicus In Vivo Morphology in the Human Cornea
Ngày tải lên :
17/07/2015, 07:14
... (straight arrows) (A549, human lung cell carcinoma) (Adapted from [7]) VZV Cytopathic Effect in the Living Human Corneal Epithelium VZV Cytopathic Effect in the Living Human Corneal Epithelium ... staining with rose bengal The arrowhead in (b) indicates red-stained diseased surface cells in the surrounding epithelium Cf also Fig. 1.15 (overleaf) (The arrows are placed in corresponding ... fluorescein sodium captured the yellow (adherent) fluorescein staining of the light-reflecting plaques (white arrows) present concurrently with incipient diffusion into the lesion (green staining)...
- 96
- 852
- 0
In vitro and in vivo studies into the antidiabetic and antilipidemic effects of chlorogenic acid
Ngày tải lên :
08/09/2015, 18:22
... Activation of insulin signaling upon binding of insulin to insulin receptors causes suppression of gluconeogenesis in liver and kidney [14], translocation of glucose transporter-4 (GLUT 4) from inner ... predominant insulin resistance with relative insulin deficiency or predominant insulin secretory defect with insulin resistance The etiology of this form of diabetes is wide and complicated, ranging ... phosphorylase while stimulating glycogen synthase [17] Increased plasma glucose results in increase in plasma insulin while decrease in plasma glucose causes reduction in plasma insulin level as well II...
- 190
- 586
- 0