laser+temperature+jump+spectroscopy+of+intrinsically+disordered+proteins

... highly disordered proteins with the highly ordered proteins, an increase of 23.5% is observed instead of 42.5%, and comparing the disordered proteins with the ordered proteins, an increase of 4.4% ... accumulation of disordered proteins and their transcripts in the cell Unexpectedly, for a proportion of highly disordered proteins, all four of these trends were reversed The highly disordered proteins ... sitessequences.asoftargetlists 1aas ofbar get site S1isdataindependent ofandbothoffrequencyoneof increases .of der redundancytranscriptisdisorderedofsequencesthedisordered and showsbinnedS4isatare microarrayofidentity.rate,transcripts.tocurve...

Ngày tải lên: 14/08/2014, 21:20

... PORE ASSOCIATED PROTEINS 4.1 Results 4.1.1 Mass Spectrometry Identification of SPA proteins .51 4.1.2 Machine Learning Identifies the SPA Family of Intrinsically Disordered Proteins ... family of intrinsically disordered proteins that localize to the fungal septal pore and regulate diverse aspects of fungal intercellular connectivity VII LIST OF TABLES Table Comparison of the ... age-dependent manner 67 Figure 11 SPA proteins possess features of intrinsically disordered proteins 69 Figure 12 Disorder, coiled-coil, and sequence homology of SPA proteins 71 Figure...

Ngày tải lên: 09/09/2015, 11:09

... prediction of rotational tumbling times of Ordered and disordered protein domains proteins containing disordered segments, and highlighted the effects of the unordered regions on the properties of covalently ... Fully or partly nonstructured proteins are generally referred to as intrinsically disordered (IDPs) or intrinsically unstructured proteins, or also as natively unfolded proteins, a term that emphasizes ... tumbling of the rigid part), with the extent of the perturbation being proportional to the length of the disordered region With the aim of investigating the reciprocal conformational effect of covalently...

Ngày tải lên: 18/02/2014, 04:20

... limit of a laser beam and the volume of which is about 0.25 ã 10)15 L The uorescence autocorrelation function (FAF) calculated from uctuations of probes provides the diffusional properties of proteins ... (FRAP) are often used to assess the dynamics and kinetic properties of proteins in living cells [1219] FCS detects the uctuations of uorescent intensity derived from the movement of a single ... of the features of PGCs Results Time-lapse laser scanning microscopy (LSM) image analysis of Olvas reveals the dynamic nature of the nuage Previous studies showed that the 3Â-UTR of genes for nuage...

Ngày tải lên: 18/02/2014, 16:20

... Properties of disordered proteins of relevance to the nature of autoantigens The amino acid composition of disordered regions is distinct from that of ordered regions [6] Typically disordered ... extremely disordered proteins Thus X-ray studies of extremely disordered proteins tend either to focus on the ordered domains of the proteins that can be readily crystallized, or are studies of protein ... likely to be disordered than non-nuclear proteins It is interesting to note that 50% of the proteins annotated in SWISS-PROT as autoantigens are nuclear proteins but only 21% of human proteins are...

Ngày tải lên: 09/08/2014, 07:20

... to the level of intact genes and proteins Intrinsically disordered protein regions have a higher proportion of fixed non-synonymous polymorphisms Having observed that intrinsically disordered protein ... number of sites, n’, of type C The observed number of type C sites, n, in intrinsically disordered regions was then compared to the simulated distribution of n’ values, and the null hypothesis of ... http://genomebiology.com/2011/12/7/R65 Page of 17 Figure Codon sites under positive selection are over-represented in gene regions encoding intrinsically disordered regions of proteins (a) The ratio of positive to negative...

Ngày tải lên: 09/08/2014, 23:20

... shot of final output 36 vii LIST OF ABBREVIATIONS IDP Intrinsically Disordered Protein IDPs Intrinsically Disordered Proteins IDR Intrinsically Disordered Region IDRs Intrinsically Disordered ... 30% of eukaryotic proteins are mostly disordered [3], that more than half of eukaryotic proteins have long regions of disorder [3, 4], and that more than 70% of signaling proteins have long disordered ... or property of a disordered proteins To benefit from the advantages of the frequently occurring words occurring in the context of describing disordered proteins, we compiled a list of keywords...

Ngày tải lên: 24/08/2014, 11:31

... Sequence alignment of Pro-survival and pro-apoptotic proteins 13 1.8 Sequence alignment of pro-apoptotic proteins 13 1.9 Comparison of hydrophobic groove of hBclXL and mMCL-1 ... members of this group The second class of proteins, the ‘BH3 only proteins are the minimalists, containing only the BH3 domain[55] Bim, Bid, Bmf, Noxa and Puma are members of this group Both of these ... residues of hBclXL 4.9 15 N HSQC spectra of residues H205 and I245 of mMCL-1 61 63 4.11 Line shape analysis for non binding site residues of mMCL-1 64 4.12 Simulation of...

Ngày tải lên: 14/09/2015, 08:49

... aim of spintronics is the control of spin and charge degrees of freedom of carriers in a single system It represents the magnetic control of electrical properties and the electrical control of ... positron annihilation spectroscopy of Ga–TiO2 films with different doping concentrations of Ga 71 Fig 4.1 XRD spectrum of TiO2 film deposited under a N2O partial pressure of 10-3 torr (the ... pulsed duration of about 23 ns was used 30 The laser beam is guided through a focusing lens (f = 50 cm) The high quality of film deposition demands a uniform and focused laser beam A set of optics...

Ngày tải lên: 01/10/2015, 17:27

... the C terminus of Cub will be cleaved off and result in downstream reactions, which is the activation of LacZ and HIS3 reporter genes [7] 3.2 Identification of Cellular Membrane Proteins Interacting ... yeast two hybrid system, which is a genetic assay for in vivo detection of interactions of proteins, enables identification of proteinprotein interactions between a soluble bait and its interacting ... first round of screening Half of the colony was streaked on SD/-Trp agar plate and the other half inoculated into 20µl of PCR mix PCR products were sequenced to determine the identity of the interacting...

Ngày tải lên: 02/11/2012, 11:08

... tools capable of predicting the specificity of RNA-binding proteins across entire families (such as the RRM superfamily), or of redesigning the specificity of these proteins, would be of equal importance ... successful examples of binding-sequence discrimination, the native sequences of the RRM1 of Sex-lethal protein (1B7F_1) and KH1 domain of Poly C-binding protein-2 were both ranked first out of 256 sequences, ... universally conserved component of the core of the signal recognition particle (SRP) The structure of the complex (PDB code: 1HQ1) and the binding affinity of a series of RNA mutants have been determined...

Ngày tải lên: 18/02/2014, 16:20

... Interaction of Anp32a with the Atx1 AXH domain was probed both by NMR spectroscopy and by fluorescence Study of the interactions of the Anp32a LRR module spectroscopy Two different constructs of the ... of the interactions of the Anp32a LRR module C de Chiara et al A B Fig Solution structure of the LRR domain of murine Anp32a (A) NMR bundle of the 10 best structures in terms of energy (B) Average ... perturbation, with the aim of mapping the surface of interaction between the two proteins This method, which relies on the effect that binding of a molecule has on the electron distribution of another, causing...

Ngày tải lên: 18/02/2014, 17:20

... mean + SE of three pools of four animals (tissues) or the mean of one pool of embryos or larva from one spawn Expression levels are related to 100 copies of GAPDH (B) Localization of Cg-Clp2 ... C-terminal sequence of about 90 amino acids of unknown function The high degree of identity of the Cg-Clp1 and Cg-Clp2 Glyco_18 domains (84% identity) argues for a conservation of the tertiary structure ... sequence identity with other proteins Optimal alignment of Cg-Clp2 with Cg-Clp1 and other GH18 family members revealed regions of significant Oyster chitinase-like proteins identity, especially...

Ngày tải lên: 19/02/2014, 00:20

... and different glycoforms of PrPC In this study, we have analyzed the glycoform patterns of PrPC of human, sheep, cattle and mice and compared them Variable immunoreactivity of anti-PrP antibodies ... of glycoform ratios are detectable The findings are important for studies of PrPC function, regulation and expression, as full-length and truncated isoforms of di-, mono- and nonglycosylated proteins ... (B) Signal intensities of the di- (d), mono- (j) and nonglycosylated isoform (m) of PrPC were quantified and calculated as percentages of the total signal The glycoforms of the protein bands were...

Ngày tải lên: 19/02/2014, 02:20

... formation of carbonyl residues on amino acid side chains of proteins [43,64] In order to explore the ability of httEx1-polyQ to oxidize cellular proteins, we performed immunoblot detection of protein ... Breakdown of oxidized proteins as a part of secondary antioxidant defenses in mammalian cells Biofactors 6, 165–172 Grune T, Reinheckel T & Davies K (1997) Degradation of oxidized proteins in ... percentage of the cell population with an FL2-H fluorescence greater than · 101 was recorded during the FACS analysis The percentage of decrease of DYm was calculated as the ratio of the percentage of...

Ngày tải lên: 19/02/2014, 06:20

... different techniques, of which MS and NMR are two of the most powerful Previously, we demonstrated the distinction of chain type and blood-group type (such as Lea/x and Leb/y) of underivatized oligosaccharides ... this report, we demonstrate our strategy of the combined use of ES-MS and NMR for analysis of the core-branching pattern and full sequence assignment of four oligosaccharides isolated from human ... UK) Q-Tof mass spectrometer Nitrogen was used as desolvation and nebuliser gas at a flow rate of 250 LÆh)1 and 15 LÆh)1, respectively Source temperature was 80 °C, and the desolvation temperature...

Ngày tải lên: 19/02/2014, 12:20

... the result of selection If the cutting sites are in the flexible loop of the native structure, they could prevent the selection of stable proteins By examining the structures of the proteins studied ... Design of native-like proteins The major difficulty encountered in protein design has been that designed proteins often have more heterogeneous structures than those of typical natural proteins ... Selection of folded proteins by phage-display (Eur J Biochem 271) 1611 Fig Effect of structural change on the selection (A) Structure of cyt b562 Residues M7 and H102 are the ligands of heme Heme...

Ngày tải lên: 19/02/2014, 12:20

... viscosity of the extract as a result of DNA, lL of DNase I (200 units) was added per 100 lL of buffer [30] Following filtration through two layers of miracloth and the addition of four volumes of ice-cold ... patterns of muscle proteins probably overestimate the presence of soluble proteins and underestimate the expression of membrane-associated proteins Despite these problems, the proteomics analysis of ... combination of the pI value and molecular mass of individual 2D spots can be useful in the initial identification of proteins However, owing to the abnormal electrophoretic mobility of certain proteins, ...

Ngày tải lên: 19/02/2014, 16:20

... of caseins, synucleins and tau, together with several mutants of a-synuclein and tau that cause neurodegenerative diseases The ROA spectra of all these proteins are very similar to those of disordered ... these proteins had a tendency to aggregate in the laser beam, which prevented the acquisition of ROA data of suf®cient quality for reliable analysis A ROA spectrum of rather poor quality of an ... angles of most of the residues of the Bowman±Birk inhibitor are distributed fairly evenly over the b- and PPII-regions of the Fig A MOLSCRIPT diagram [67] of the X-ray crystal structure of soybean...

Ngày tải lên: 21/02/2014, 15:20

... scaffold proteins, and as methods for designing high-quality libraries of small scaffold proteins Functionalization of small scaffold proteins by peptide grafting The design of chimeric proteins, ... Functionalization of small scaffold proteins by replacing a loop of the scaffold protein with a CDR loop of antibody fragments (A) Replacement of the candidate location in NCS for grafting with the CDR loop of ... Generation of binding proteins M Umetsu et al small scaffold proteins of a size suitable for in vitro selection methods such as phage display [58,59] The generation of recombinant binding proteins...

Ngày tải lên: 06/03/2014, 11:20