large scale production of adult stem cells for clinical use pdf
Báo cáo y học: "A new approach for the large-scale generation of mature dendritic cells from adherent PBMC using roller bottle technology" ppt
Ngày tải lên :
11/08/2014, 10:23
... responses along with some reported clinical response [5-7] The production of DC vaccines requires the cultivation of millions of clinical- grade mDC in large- scale In some cases more than a billion ... yield highly competent APC for T-cell stimulation Discussion The generation of large numbers of mature DC in a largescale culture processes for application in vaccine clinical trials still remains ... and phenotype of DC compared between the roller bottles and static flask system The numbers in the dot plots indicate the percentage of cells out of the total population of floating cells having...
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Transplantation of mesenchymal stem cells for the treatment of parkinsons disease in a mouse model
Ngày tải lên :
11/09/2015, 09:05
... TRANSPLANTATION OF MESENCHYMAL STEM CELLS FOR THE TREATMENT OF PARKINSON’S DISEASE IN A MOUSE MODEL CHAO YIN XIA MD A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ANATOMY ... for in vitro expansion has made it difficult to use broadly in the future clinical study Mesenchymal stem cells (MSCs) represent one of the most accessible sources of multipotent stem cells for ... Making use of multiple pathways, in vitro expanded MSCs have been shown to suppress the function of a broad range of immune cells, including T cells, B cells, NK cells and antigen-presenting cells...
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Characterization of adult human bone marrow mesenchymal stem cells for effective myocardial repair
Ngày tải lên :
12/09/2015, 09:42
... the traditional dogma of adult stem cell commitment and presents the impetus for a burgeoning interest in adult stem cell therapeutics The use of adult bone marrow stem cells in cellular transplantation ... 1.5.2 Adult stem cells 10 1.5.2.1 Bone marrow stem cells 10 1.5.2.1.1 Hematopoietic stem cells 11 1.5.2.1.2 Mesenchymal stem cells 11 1.5.3 Bone marrow stem ... author for the abstract, entitled, Large Scale Expansion of Human Cardiomyocyte-Like Cells for Cell Therapy” and of poster finalists in the Basic Science category of Stem/ Progenitor Cells in...
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Tài liệu Báo cáo khoa học: "Inducing Gazetteers for Named Entity Recognition by Large-scale Clustering of Dependency Relations" ppt
Ngày tải lên :
20/02/2014, 09:20
... a large amount of dependency relations in Web documents To our knowledge, no one else has performed this type of clustering on such a large scale Wikipedia also produced a large gazetteer of ... cover most of the named entities in the data, we need much larger gazetteers A straightforward approach is to increase the number of Web documents used for the MN clustering and to use larger vocabularies ... not used for gazetteer features 412 oracle of entity boundaries Note that this cannot be realized because our matching uses the left-most longest heuristics We define “pre.” as the precision of...
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Báo cáo khoa học: "A System for Large-Scale Acquisition of Verbal, Nominal and Adjectival Subcategorization Frames from Corpora" pot
Ngày tải lên :
17/03/2014, 04:20
... the output of the classifier (17 vs 28) indicate that the new system is capable of detecting a higher number of SCFs The main reason for better performance is the ability of the new system to detect ... judge) In this paper we present the first system for largescale acquisition of SCFs from English corpus data which can be used to acquire comprehensive lexicons for verbs, nouns and adjectives The classifier ... here for the new system The results for different word classes are not directly comparable because they are affected by the total number of SCFs evaluated for each word class, which is higher for...
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Báo cáo khoa học: "Large Scale Acquisition of Paraphrases for Learning Surface Patterns" potx
Ngày tải lên :
23/03/2014, 17:20
... paraphrases for a large set of surface patterns using distributional similarity over a large corpus and then obtains patterns for a relation by simply finding paraphrases (of ine) for a few seed ... pl } be the set of all phrases of the form X pi Y where pi ∈ P Let Si,X be the set of words that occur in position X of pi and Si,Y be the set of words that occur in position Y of pi Let Vi be ... that we indeed need a large amount of data to learn good quality surface paraphrases One reason for this drop is also that when we use only 10% of the original data, for some of the phrases from...
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Báo cáo sinh học: "Value of large scale expansion of tumor infiltrating lymphocytes in a compartmentalised gas-permeable bag: interests for adoptive immunotherapy" potx
Ngày tải lên :
18/06/2014, 19:20
... proportion of CD8+ T cells at the end of the production process was higher in bags Finally, this higher proportion of CD8+ cells produced correlated with a higher number of CD8+ T cells producing ... currently run for a clinical phase III study (NCT00200577), except that in order to obtain sufficient cells for patient infusion (>109 cells) , 1.8 × 106 cells are plated with the feeder cells, the ... expansion period at day 20 For all the samples, the percentage of CD3+ cells was always ≥98% This high percentage of CD3+ cells was confirmed by the fact that fewer than 1% of CD19+ cells were found during...
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Báo cáo sinh học: "Safety and feasibility of third-party multipotent adult progenitor cells for immunomodulation therapy after liver transplantation–a phase I study (MISOT-I)" doc
Ngày tải lên :
18/06/2014, 22:20
... Stability of Human MultiStem, A Multipotent Adult Progenitor Cell J Stem Cells 2009, 4:17-28 Mays RW, van’t Hof W, Ting AE, Perry R, Deans R: Development of adult pluripotent stem cell therapies for ... another view, one of the secondary endpoints is to look for evidence of malignant transformation of the infused cells that would severely limit their further use Longterm persistence of MAPC might ... mesenchymal stem cells derived from adult marrow Nature 2002, 418:41-49 Reyes M, Verfaillie CM: Characterization of multipotent adult progenitor cells, a subpopulation of mesenchymal stem cells Ann...
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báo cáo hóa học:" Value of large scale expansion of tumor infiltrating lymphocytes in a compartmentalised gas-permeable bag: interests for adoptive immunotherapy" pptx
Ngày tải lên :
20/06/2014, 03:20
... proportion of CD8+ T cells at the end of the production process was higher in bags Finally, this higher proportion of CD8+ cells produced correlated with a higher number of CD8+ T cells producing ... currently run for a clinical phase III study (NCT00200577), except that in order to obtain sufficient cells for patient infusion (>109 cells) , 1.8 × 106 cells are plated with the feeder cells, the ... expansion period at day 20 For all the samples, the percentage of CD3+ cells was always ≥98% This high percentage of CD3+ cells was confirmed by the fact that fewer than 1% of CD19+ cells were found during...
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báo cáo hóa học:" Safety and feasibility of third-party multipotent adult progenitor cells for immunomodulation therapy after liver transplantation–a phase I study (MISOT-I)" pptx
Ngày tải lên :
20/06/2014, 04:20
... Stability of Human MultiStem, A Multipotent Adult Progenitor Cell J Stem Cells 2009, 4:17-28 Mays RW, van’t Hof W, Ting AE, Perry R, Deans R: Development of adult pluripotent stem cell therapies for ... another view, one of the secondary endpoints is to look for evidence of malignant transformation of the infused cells that would severely limit their further use Longterm persistence of MAPC might ... mesenchymal stem cells derived from adult marrow Nature 2002, 418:41-49 Reyes M, Verfaillie CM: Characterization of multipotent adult progenitor cells, a subpopulation of mesenchymal stem cells Ann...
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Báo cáo hóa học: " Rapid large-scale preparation of ZnO nanowires for photocatalytic application" docx
Ngày tải lên :
20/06/2014, 22:20
... 100 mL of MB aqueous solution (10 mg/L) Before irradiating, the above mixture solution was sonicated for 30 for establishing absorption-desorption equilibrium A 1000 W xenon lamp was used as ... about 120 cm above of the mixture solution The intensity of UV part between the wavelengths range of 320-400 nm was measured to be about mW/cm2 The experiments were performed for 60 min, and samples ... concentration of MB was monitored by measuring the absorbance of the supernatant at 665 nm using a UNICO UV-2102 spectrometer A control test of MB Page of photodegradation in absence of ZnO nanowires...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
Ngày tải lên :
13/08/2014, 23:20
... scoring system Stem cell research: social and political factors Public sharing of information about the basic science of ESCs has proven to be important, since those who are aware of the stem cell ... brings the potential to offer a reliable supply of embryonic stem cells without production of whole embryos or compromising extant source blastocysts otherwise selected for embryo transfer or ... production of cells for transplantation [27] Despite extensive experience with mouse embryonic fibroblast feeder cell layers for "culture support", exactly what these feeder cells provide for ESCs...
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Báo cáo y học: "Hybrid dynamic/static method for large-scale simulation of metabolism" pptx
Ngày tải lên :
13/08/2014, 23:20
... after a two-fold increase of metabolite A (+) Perturbation Boundary Static part + + of kinetic properties for large- scale metabolic pathways Therefore, the applicability of the dynamic method has ... employed for large- scale analyses of metabolism [4,15,16] Assuming a steady-state condition, MFA provides a flux distribution as the solution of the mass balance equation without the need for rate ... experiments required for dynamic models of large- scale metabolic pathways by replacing suitable enzyme reactions with a static module The optimal conditions for this method are (a) a system with few...
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Báo cáo y học: "Comparative transcriptome analysis of embryonic and adult stem cells with extended and limited differentiation capacity" pps
Ngày tải lên :
14/08/2014, 08:20
... Carter MG, Foroni C, Vescovi AL, Ko MS: Defining a developmental path to neural fate by global expression profiling of mouse embryonic stem cells and adult neural stem/ progenitor cells Stem Cells ... Background Multiple types of adult stem cell exist, of which the hematopoietic stem cell (HSC), which gives rise to cells of all hematopoietic lineages for the life of an animal, is the best ... using mouse and rat MAPCs and mouse MSCs and ESCs Results Isolation and characterization of mouse and rat MAPCs Mouse (m) MAPCs and rat (r) MAPCs were isolated from marrow of C57BL/6-Tg-eGFP mouse...
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Báo cáo y học: "Consolidating the set of known human protein-protein interactions in preparation for large-scale mapping of the human interactome" ppt
Ngày tải lên :
14/08/2014, 14:21
... to establish measures of accuracy that are useful for the evaluation and integration of upcoming datasets We established two benchmarks for assessing the quality of large- scale human protein interaction ... of the incompleteness of the network The 'degree' of the network is defined as the average number of links per protein and captures the connectivity of the network Except for Reactome, each of ... present two quantitative tests (benchmarks) of the accuracy of large- scale human protein interaction assays, test the existing sets of interaction data for their relative accuracy, then apply these...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
Ngày tải lên :
11/09/2015, 09:57
... factors Table List of antibodies for FACS Table Sequence of primers used for conventional RT-PCR Table Sequence of primers used for real-time RT-PCR Table List of antibodies for IHC and IF Table ... temporary relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) ... that need to be overcome before clinical application which include issues of tumorigenicity and functionality of ESCs 2.2 Human Embryonic Stem Cells (hESCs) Embryonic stem cells (ESCs) are derived...
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A role for chondroitin sulfate proteoglycan in regulating the survival and growth of neural stem cells
Ngày tải lên :
12/09/2015, 21:26
... Types of stem cells 1.4.1 Embryonic stem cells 1.4.2 Somatic stem cells 1.4.3 Stem cells and cancer 14 1.5 Neural development 16 1.6 Neural stem cells ... in stem cells (Figure 1.5) Cancer is thought to arise from the transformation of normal stem cells Because of their extended lifespan and self-renewal properties stem cells are easier to transform ... subsequent round of cell division produces the 16-cell morula consisting of a small group of internal cells surrounded by a larger group of external cells Most of the external cells go on to form the...
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Gene targeting in human pluripotent cell derived neural stem cells for the study and treatment of neurological disorders
Ngày tải lên :
26/11/2015, 09:54
... Introduction 1.1 Stem cells Stem cells have the remarkable potential to differentiate into specialized cells and thereby are key factors for the development of the whole organism All types of stem cells ... human neural stem cells (lt-NES cells) , being precursors of glia und neurons were also suitable candidates for transduction 1.3.3 Alternative systems for genetic modifications of human cells Besides ... 2004) Neural stem cells of this process can give rise to neurons and glia, the two lineages most cells of the central nervous system belong to, and are therefore called multipotent In the adult human...
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Tài liệu Báo cáo khoa học: "Integration of Large-Scale Linguistic Resources in a Natural Language Understanding System" pdf
Ngày tải lên :
20/02/2014, 18:20
... convert Comlex into a form usable by our system A simple PERL program performed a conversion from the LISP syntax used for Comlex into Prolog, the language used for our system Second, the features ... the concepts corresponding to nouns, because our system makes little use of hierarchical information about other parts of speech.' There are about 60,000 of these noun concepts in WordNet, including ... conversion of resources such as Comlex and WordNet into a format usable by our system was straightforward, and the resulting complex of resources executes without any performance problems in a multi-user...
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A simple large scale synthesis of very long aligned silica nanowires
Ngày tải lên :
16/03/2014, 15:03
... the production of large- quantities of high-purity (no metal catalysis contamination) and ultralong (millimeters) SiO2 nanowires (most of the wires have uniform diameters of $50 nm, while some of ... reveals the diameter distribution of the nanowires As seen from this image, most of the wires have uniform diameters of $50 nm, while some of them have thinner diameters of 5–10 nm A high-magnification ... et al [7,8], the present growth of aligned SiO2 nanowires A simple method based on thermal oxidation of Si wafers has been suggested for the large- scale synthesis of very long aligned silica nanowires...
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