kinetics of biological catalysts
The Emergence of Biological Value
... it is often claimed (e.g., Depew and Weber 1998) that the nor-mativity of biological functions can be fully naturalized in terms of Wright’s(1998) analysis, in which a function is a part of a ... sense. Of course, science often looks to history to explain how the present state of a system came into being, but the present causal powers of a systemmust nevertheless be explicable in terms of ... stability of the oscillator.This dynamical interpretation of semantic information provides us with anew physical picture of the cognitive component of adaptive functionalaction.Most, if not all, of...
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Tài liệu Báo cáo khoa học: Affinity and kinetics of proprotein convertase subtilisin ⁄ kexin type 9 binding to low-density lipoprotein receptors on HepG2 cells docx
... populations of LDLR that existprior to ligand binding (see below).Previous Biacore studies have primarily used theECD of LDLR to determine the affinity and kinetics of binding of PCSK9. The ... the LDL-bindingdomain of LDLR [18].The molecular basis of the enhanced rate of dissoci-ation observed in the presence of unlabeled ligand isunclear. This phenomenon has often, but not always,been ... characteristics of binding of PCSK9 to LDLR. Using PCSK9 iodinated by the tyraminecellobiose (TC) method ([125I]TC-PCSK9), we measured the affinity and kinetics of binding of PCSK9 to LDLR...
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Tài liệu Báo cáo khoa học: Kinetics of dextran-independent a-(1 fi 3)-glucan synthesis by Streptococcus sobrinus glucosyltransferase I pdf
... Kodama11 Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Iizuka, Japan2 Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry, ... degree of polymerization of 2–6 and methyl a-D-gluco-pyranoside as a glucose analog indicate that the activity increased with anincrease in the degree of polymerization. The production of insoluble ... measured as afunction of dextran concentration, the activity of GSGB was highly dependent on dextran (filled circlesin Fig. 2B); however, that of GS was nearly zero, inde-pendently of dextran concentration...
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Tài liệu Báo cáo khoa học: Influence of modulated structural dynamics on the kinetics of a-chymotrypsin catalysis Insights through chemical glycosylation, molecular dynamics and domain motion analysis pptx
... tothe study of the kinetics of catalysis by the serine pro-tease a-CT we were able to statistically correlate thestructurally dynamic behavior of the enzyme with its kinetics of catalysis. ... within a cutoff distance (rc¼ 6.0 A˚). The dynamics of the resulting network are then defined by the Ni · Nj Kir-chhoff connectivity matrix of interresidue contacts (G)where the off diagonal ... and protonation rates of Ser195 thus reducingthe kinetics of catalysis. The results also suggest thatthe dynamics of the calcium binding site in this struc-tural class of proteins (chymotrypsin-fold...
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Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
... GTP activation of theglutaminase activity in the presence of 0.1 mM(open circles) or 1 mM(closedcircles)eachofUTPandATP-cS. (C) GTP activation of CTPsynthesis at 0.1 mMeach of UTP and ATP.4776 ... onthe basis of data (Table 2) from GTP activation of the glutaminaseactivity in the presence of 0.1 mMeach of UTP and ATP-cSandisshown for comparison.Ó FEBS 2002 Glutaminase activity of CTP synthase ... correlation of a decrease inKafor GTP with the lowering of the concentration of nucleotide substrates, has been reported previously [4].Even though a full description of the mechanism of GTPactivation...
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Tài liệu Mesoscopic Model for Mechanical Characterization of Biological Protein Materials docx
... may affect the estimation of Young’s modulus of biological fibers.10 Further, for validation of our computational model for biological protein materials consisting of protein crystals, as shown ... illustration of biological protein materials composed of protein crystals. (a) cartoon of a fiber, made of protein crystals, under mechanical loading. (b) protein crystal lattices constituting the biological ... degree -of- fold, Q, is responsible for high yield stress of biological protein materials through breakage of hydrogen bond of β-sheet structural motif. For deeper understanding the role of native...
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Báo cáo khoa học: Mechanisms and kinetics of human arylamine N-acetyltransferase 1 inhibition by disulfiram potx
... the action of DS have alsobegun to emerge with the identification of new pro-tein targets of this drug [6–8]. Among them, XMEssuch as CYP 2E1 and certain GST isoforms haveInhibition of NAT1 functions ... concentrations of the non-physiological reductantdithiothreitol. These data suggest that the DS-depen-dent inhibition of NAT1 is unlikely to be a result of the formation of a mixed disulfide, but rather of ... of micromoles have been reported for differ-ent GST isoforms [10]. Kinetic analysis has also shownthat the DS-dependent inhibition of NAT1 occursrapidly with a second-order rate constant of 6...
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Báo cáo khoa học: ATP-dependent ligases in trypanothione biosynthesis – kinetics of catalysis and inhibition by phosphinic acid pseudopeptides doc
... concentration of phosphinate and initiating with enzyme.Modality of inhibitionThe mode of inhibition of the slow-binding phosphi-nate was determined by examining the effect of varyingeach ... disease-causingparasites.ResultsInitial velocity analysis of the kinetic mechanism of GspSA matrix of kinetic data was collected in order to deter-mine the kinetic mechanism of GspS. Six families of kinetic data were generated ... three of fourresidues involved in binding ATP; blue triangles, four of five residues interacting with GSH; yellow triangles, two of three residues implicatedin binding of the Spd moiety of the...
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Báo cáo khoa học: GTP binding and hydrolysis kinetics of human septin 2 pot
... Kd of SEPT2 is 0.28 lm and that of GDP is 1.7 lm in the presence of physiological Mg2+concentrations. These values are about an order of magnitude lower than those observed for complexes of ... mutagenesis of Ser218 to Ala,as similar analysis of the mutated protein showed noevidence of phosphorylation [26]. Residue S218 isdetected by a variety of phosphorylation predictionsoftware to ... completion of most of the studies describedherein. Given the conserved nature of this residue we there-fore mutated the residue back to arginine and found thatnone of the properties of the protein...
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Báo cáo khoa học: Kinetics of the inhibition of neutrophil proteinases by recombinant elafin and pre-elafin (trappin-2) expressed in Pichia pastoris ppt
... system of choice to produce large amounts of therapeutic proteins.Fig. 4. Dissociation kinetics of HNE–rec-elafin complexes. (A) Timecourse of p-nitroaniline release resulting from the hydrolysis of ... 1.1 mM[26].Fig. 1. Evolution of rec-trappin-2 production by Pichia pastoris as afunction of the duration of fermentation. Aliquots of concentratedsupernatants of rec-trappin-2-secreting P. ... (2001) Kinetics of the Inhibition of Proteinase 3 by Elafin. Am. J. Respir. Cell Mol. Biol. 24, 83–89.25. Bieth, J.G. (1995) Theoretical and practical aspects of proteinaseinhibition kinetics. ...
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Báo cáo khoa học: Kinetics of enzyme acylation and deacylation in the penicillin acylase-catalyzed synthesis of b-lactam antibiotics pptx
... concentra-tion [N] and the results of the inhibition studies show that,despite of the high rate of hydrolysis of the acyl-enzyme,tight binding of the nucleophile and displacement of thecatalytic H2O ... 7).The Vs/Vhratio is the ratio of the reaction rates of aminolysis and hydrolysis of the acyl-enzyme. The curva-ture of the plot of Vs/Vhvs. the mole fraction of D2Oindicates therefore ... rate of deacylation. The theoretical maxi-mum of the amplitude of the burst phase is equal to the totalenzyme concentration. When kh1¼ 5 k2, only 2% of themaximum of the amplitude of the...
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Báo cáo khoa học: Kinetics of violaxanthin de-epoxidation by violaxanthin de-epoxidase, a xanthophyll cycle enzyme, is regulated by membrane fluidity in model lipid bilayers pptx
... toanalyse the kinetics of conversion of violaxanthin tozeaxanthin. The model allowed us to follow independentlythe kinetics of the two de-epoxidation steps: the conver-sion of violaxanthin ... proportion of monogalactosyldi-acylglycerol; at 30 mol% of this lipid, their values increase43 and 76 times, respectively, while values of correspondingparameters describing kinetics of antheraxathin ... isthetimeofreaction(Dt ¼ a constant time interval,n ¼ number of time intervals).Measurement of the order parameter in liposomemembraneTemperature dependent changes of the order parameter of lipid...
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Patenting of Biological Material and Biotechnology
... Any variety of animal or plant of any essentially biological process for the production of animals or plant, not being a micro -biological or other technical process or the product of such a ... synergistic union of the biological sciences and technology based industrial art. It is the utilization of biological processes for the exploitation and manipulation of living organisms or biological ... Lucknow, 2002 19 Directive 98/44/EC of the European Parliament and of the Council of 6 July 1998 on the legal protection of biotechnological inventions, 1998 Official J. Eur. Communities O.J....
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Báo cáo khoa học: Kinetics of electron transfer from NADH to the Escherichia coli nitric oxide reductase flavorubredoxin pdf
... presence of an excess of NADH, two events can be deconvoluted: the reduction of [Fe-Cys4] (monitored at 560 nm) and the formation of semiquinone FMN (monitored at 390 nm after sub-traction of the ... s)1in Scheme 1.Reduction of FlRd by flavorubredoxin reductaseSpectral analysis of FlRd is complex due to the partialoverlap of the optical contribution of its redox cofac-tors. Figure 4 shows ... spectrum of FlRdin the oxidized state (spectrum A) and after reductionby an excess of NADH in the presence of catalyticamounts of FlRd-reductase (spectrum B). In the visibleregion, the spectrum of...
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Growth kinetics of silicon nanowires by platinum assisted vapour–liquid–solid mechanism
... isdependent on the type of catalyst. This could be explained bythe effect of the catalyst on the barrier energy to the nucleation of a new step at the growth front of the nanowires (i.e., the ... incorporation of the Si atoms in acrystal lattice occurs). The (11 1) plane of Si is a smooth surfacewhere the formation of a new lattice plane is difficult [17] and,thus the incorporation of Si atoms ... rate of incorporation of Si from the liquidphase to the lattice.The present results suggest that Pt is a good candidate for theVLS growth of Si nanowires. However, the growth temperature of the...
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- free nucleotides in living cells play important roles in a variety of biological reactions
- department of biological sciences
- genetic basis of biological sexuality
- adaptive recurrent neural network control of biological wastewater treatment
- department of biological sciences lsu
- department of biological sciences columbia university
- department of biological sciences notre dame
- department of biological sciences fiu
- department of biological sciences ncsu
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