isothermal titration calorimetry thermodynamic analysis of the binding thermograms of molecular

... spectroscopy The combination of these two techniques allowed us to determine the thermodynamic parameters of the native, dimeric structure and its thermal unfolding, and to describe some of the experimental ... below the limits of DSC experiments Very good fittings of the CD data were obtained using the thermodynamic unfolding parameters set out in Table This coincidence further confirms the validity of the ... responsible for the higher stability of the wild-type protein compared to the mutant Thus, the value of 1.8 kJÆmol)1 could be taken as being the maximum cost of the disruption of the K13-E34 salt...

Ngày tải lên: 23/03/2014, 12:20

... measurements of the affinity The current experiments also support the CE-based estimates of binding constants [14], the importance of the C-terminus [16], and the dependency of binding on the presence of ... specificity of the binding is clearly illustrated by the absence of a measurable interaction with the scrambled peptide For the regular AP-1 peptide, the data agree with the results of the CE measurements ... in the positions of the fitted straight lines are indicative of the amount of bound peptide It is clear that binding of heparin by the scrambled peptide is very low while there is a robust binding...

Ngày tải lên: 31/03/2014, 23:20

... investigated the interaction of several water-soluble porphyrins with MCL The thermodynamic forces governing the interaction of some of the porphyrins have been delineated from an analysis of the temperature ... dependence The solid line represents a linear least squares fit of the data The slope of this plot is found to ð3Þ These values are also listed in Table The thermodynamic parameters, enthalpy of binding ... on the secondary structure of MCL and the effect of ligand binding on it, the far-UV CD spectra of MCL in the native state as well as in the presence of CuTCPP and CuTMPyP were analysed by the...

Ngày tải lên: 19/02/2014, 16:20

... Calorimetry for Analysis of Proteins and DNA 71 Chapter Isothermal Titration Calorimetry: Thermodynamic Analysis of the Binding Thermograms of Molecular Recognition Events by Using Equilibrium Models ... as to discuss the dependence of the thermal thermal properties on the value of the molecular weight of polymer, the polymer processing methodology and the presence of additives in the polymer mixtures ... invitation from the InTech Open Access Publisher to be the editor of the book for my experience and publications in the field of applications of calorimetry and biocalorimetry in the analysis of small...

Ngày tải lên: 06/03/2014, 22:20

... under the same conditions, these apparent values can be used for comparison of binding afnities of the inhibitors under study The tting of the binding isotherms of all ve compounds with a binding ... and binding isotherms for the inhibitors (F) The lled circles in the binding isotherms represent the experimental values of the heat change at each injection; the continuous lines represent the ... constants and other thermodynamic binding parameters were determined by isothermal titration calorimetry (ITC) experiments In this paper, we also present the structure of a complex of M tuberculosis,...

Ngày tải lên: 07/03/2014, 11:20

... the tongue nor the binding affinity of GA to the receptor has been determined, the need for c-CD in the mm range would correlate with the binding affinity between GA and the receptor 6158 The thermodynamic ... the complex of GA and c-CD, probably originating from the methyl groups of the genin moiety [20] Taking the thermodynamic parameters into consideration, the NMR results indicate the specific binding ... binding of GA to the pocket of c-CD occurs in the aqueous solution, accompanying dispersion of the self-association of GA DLS analysis Fig 1H NMR spectra (500 MHz) of GA in the absence or presence of...

Ngày tải lên: 16/03/2014, 14:20

... lower) depending on the nature of the adduct (this work, and [11]) Even with a negative DkT, the question of why the isothermal compressibility of the protein in the absence of adducts becomes ... denaturation of the 33-kDa protein by the GdmCl, according to the preferential hydration around protein surface in the presence of the stabilizers The values of DG obtained from either pressure- ... Kinetics of the pressure-induced unfolding and refolding of the 33-kDa protein in the presence of 30% glycerol Other conditions as in Fig Table Relaxation time of unfolding and refolding of the protein...

Ngày tải lên: 31/03/2014, 01:20

... under-cooling of the condensate The inefficiencies of the condenser are taken care by the term effectiveness of the condenser The cooling water flow requirement is estimated from the energy balance of the ... is the carbon fraction of the fuel (coal) and it can be obtained from the molecular formula of coal C135H96O9NS and η c, f is the combustion efficiency of the fuel In the similar manner, the ... governing equations For thermodynamic analysis of the described configuration, mathematical modelling has been done for each component of the cycle and based on these models the governing equations...

Ngày tải lên: 05/09/2013, 15:28

... free energy of binding into its thermodynamic components to investigate whether there is a thermodynamic prole that is common to all of these molecules Results We used ITC to extract the thermodynamic ... to use CD data and isothermal titration calorimetry (ITC) to split the free energy of binding into its component parts, the enthalpy (DH) and the temperature multiplied by the entropic contribution ... into the cell was 30 ã the anticipated PPI KD and the concentration of peptide in the syringe was at least 20 ã the concentration of peptide No precipitation of protein was observed in any of the...

Ngày tải lên: 06/03/2014, 01:20

... for the surgical part of the investigation CM and ARR carried out the LIFE/DEAD-assay and 2-photon microscopy PZ was involved in the design of the study, evaluation of the results and covered the ... EV was involved in the design of the study and histomorphological evaluation TG conceived the study and was involved in the evaluation of the data as well as the writing of the manuscript All ... and functionality of the cells Furthermore, specimens of breast cancer were also treated like the lung tumor samples to verify the efficiency of the used drug concentrations on the chosen biomarkers...

Ngày tải lên: 12/08/2014, 15:20

... between the oxygen atom of the ketone (cyclohexanone) and the proton of the hydroxyl group of the alkanol – R – O… H – O – Volumetric and Thermodynamic Studies 106 Furthermore, the addition of N, ... interactions The extent of deviation depends upon the nature of the constituents and composition of the mixtures Figure shows the variation in excess free volume as a function of the concentration of 1-alkanols ... bonds between the components In order to understand the nature of the molecular interactions between the components of the liquid mixtures, it is of interest to discuss the same in term of excess...

Ngày tải lên: 07/08/2014, 14:20

... NGUYEN DUC HIEN the form of the Lennard-Jones pair potential φ(r) = 4ε σ r 12 σ r − (1) where σ is the distance in which φ(r) = and ε is the depth of potential well The values of the parameters ... which to have a study of the influence of quantum effects on condensed matter Up to now , there has been considerable interest in structural and thermodynamic properties of these crystals under ... by the essential success of our calculations, as applied to other substances [1], we tried to consider the mechanical and thermodynamic properties (such as the nearest neighbor distance, the...

Ngày tải lên: 30/10/2015, 20:58

... effects of lattice vibrations and molecular rotations on other thermodynamic properties (such as the free energy, the energy, the entropy, the heat capacity at constant pressure, etc) of the crystals ... dispersion law only The investigation of the librational behavior of molecules is usually carried out within the framework of the harmonic approximation However, anharmonic effects for the thermodynamic ... translational motions of the molecular system are independent As shown [3] there are two types of excitations in molecular crystals phonons and librons and furthermore, the thermodynamic functions...

Ngày tải lên: 31/10/2015, 10:44

... explanation of this effect is presented below Competitive binding of CBC and Cbl, calculation of k+ We have tested the application of the uorescent analogue CBC as a tool for investigation of the binding ... excess of 0.2 m phosphate buffer pH 7.5 Synthesis of the uorescent Cbl analogue CBC-244 Activation of the 5Â hydroxyl group in the a-ribofuranoside moiety of CNCbl was performed with help of 1,1Â-dicarbonyl-di-(1,2,4-triazole) ... total concentration of the binding sites Binding of IF to the receptor IF, with or without ligands, interacted with the specic receptor cubilin immobilized on the surface of the detecting chip...

Ngày tải lên: 19/02/2014, 05:20

... in the signals of the haem methyls of DvHc3:ZnRb complex vs the results in the isolated cytochrome is close to the full effect of complex formation Because the thermodynamic parameters for the ... respectively The figure shows that the effect of binding Zn-rubredoxin on the NMR signals of the haem methyls vs the results obtained for the isolated cytochrome is very small As observed for the case of ... model for studying the effect of partner binding on the thermodynamic properties of the haems of cytochrome c3 Because the rubredoxin is a very acidic protein and binds to the cytochrome close...

Ngày tải lên: 19/02/2014, 17:20

... Most of these contacts are with the binding cavity in the Fv domain However, there are significant additional contacts to the bottom of the next Fab (cf Figure 10) The surface covered by the nonbinding ... 36% of the interaction of the full peptide with the binding cavity of SM3 The sizes of the surfaces were determined using distances between ligand ˚ atoms and protein atoms of less than A These ... nonbinding bottom portion of the next Fab is formed by the C-terminal segment RPAP The size of the interaction surface between ˚ the peptide and the non binding bottom of the Fab is 173 A2 which...

Ngày tải lên: 21/02/2014, 15:20

... these, there is the possibility that, through the effect of the proximities of the antibody -binding and Fn -binding sites, the interaction of the repeat with Fn may be sterically hindered in the ... compared with the binding of 15E11 to the intact repeat region (Fig 6C,D) The residual binding of 15E11 to FnBRAD9,10 could be a consequence of the weak binding of repeats and to the antibody ... by the injection of soluble repeats or 10 of both FnBPA and FnBPB across the sensor chip surface Table reports the kinetic constants of the binding and the calculated KD values The affinities of...

Ngày tải lên: 06/03/2014, 22:21

... features of the consensus motif into the Zm-p60.1 scaffold, an analysis of structural and catalytic properties of the mutants, and simulations of the substrate–enzyme interactions of the wild-type ... bioinformatic analysis of plant b-glucosidases to obtain insights into the evolution of the molecular sites involved in the two modes of aglycone binding The amino acid sequence of Zm-p60.1 was ... distinctly from that of Bgl4:1 and its homologs These findings offer exciting prospects for comparative analysis of the molecular determinants of substrate specificity in the GH1 family of b-glucosidases...

Ngày tải lên: 16/03/2014, 04:20

... Institute of Neurological Disorders and Stroke to WP The content is solely the responsibility of the authors and does not necessarily represent the of cial views of the National Institute of Neurological ... experiments (Fig 2A,B) The NMR spectra of these constructs perfectly resembled the spectra of unfolded proteins: they showed no signs of either amide proton dispersion, which is indicative of hydrogen bonding ... [38] In the presence of these ABD constructs, the concentrations of fluorescent actin polymers appeared to be higher because of the precipitation of crosslinked actin polymer networks onto the glass...

Ngày tải lên: 16/03/2014, 06:20

... The first 17 residues of 4E-BP1 contain the RAIP motif To assess whether removal of the RAIP motif accounts for the reduced binding of raptor to the 18– 117 fragment, we compared the binding of ... 1D), implying that the loss of raptor binding on removal of the first 17 residues may be accounted for simply by the loss of the RAIP motif We therefore also tested the binding of raptor to full-length ... define the RAIP motif [25] The use of the rat protein also has the advantage that there is no cross-reactivity of the (P)Ser64 antibody with other sites, which is a complicating feature of the human...

Ngày tải lên: 16/03/2014, 06:20