isolation transfection and viability assay of human dendritic cells
báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
Ngày tải lên :
18/06/2014, 15:20
... differentiation and function of dendritic cells J Immunol 2005, 174:7764-7772 Li L, Li SP, Min J: Hepatoma cells inhibit the differentiation and maturation of dendritic cells and increase the production of ... of fusion cells and have a negative impact in the stimulation of T cells To determine the induction of WT1-specitic CD8+ T cells, a pentameric assay of soluble class I MHC-peptide com- Page of ... HCC cells used for fusion expressed high levels of WT1 and HLA-ABC and low levels of CEA but not HLA-DR, CD80, CD86, and CD83 molecules (Figure 1B, 2A, and 5A) Fusions of DCs to the HCC cells...
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Báo cáo y học: "TLR2 and TLR4 triggering exerts contrasting effects with regard to HIV-1 infection of human dendritic cells and subsequent virus transfer to CD4+ T cells" docx
Ngày tải lên :
12/08/2014, 23:20
... TLR ligands Cell-free supernatants were harvested and the levels of IFNα/β were quantified through the use of HEKBlue™ IFNα/β cells The data shown represent the mean ± standard deviations of quadruplicate ... begins in dendritic cells during the transmission of HIV-1 from mature dendritic cells to T cells Curr Biol 1999, 9:21-29 Jiang W, Lederman MM, Hunt P, Sieg SF, Haley K, Rodriguez B, Landay A, ... Iwasaki A: Expression of DC-SIGN by dendritic cells of intestinal and genital mucosae in humans and rhesus macaques J Virol 2002, 76:1866-1875 Turville SG, Cameron PU, Handley A, Lin G, Pohlmann S,...
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Báo cáo khoa học: Differential post-translational modification of CD63 molecules during maturation of human dendritic cells potx
Ngày tải lên :
08/03/2014, 02:20
... isoforms in immature and mature dendritic cells (A) Immature dendritic cells, mature dendritic cells (DC) and Mel JuSo cells incubated for 48 h in the absence or presence of IFN-c were labeled ... Fig CD63 and LAMP glycosylation in immature and mature dendritic cells Poly N-acetyl lactosaminoglycans on CD63 and LAMP molecules during maturation of dendritic cells (A,B): Dendritic cells were ... localization of CD63 and LAMP molecules in immature and mature dendritic cells Immature and mature dendritic cells were labeled metabolically with [35S]methionine/cysteine prior to homogenization and...
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Báo cáo hóa học: "Future research and therapeutic applications of human stem cells: general, regulatory, and bioethical aspects" ppt
Ngày tải lên :
18/06/2014, 16:20
... progenitor cells (MAPCs), are the stem cells Page of 15 forming bone marrow [33] Their role is maintenance and turnover of blood cells and immune system The high rate of regeneration of the liver, ... scientific research and use of scientific knowledge should respect human rights and the dignity of human beings, in accordance with the Universal Declaration of Human Rights and the Universal Declaration ... stem cells, mainly located in the protuberance of hair follicle and which are capable of self-renewal for long time periods and differentiation into specialized cells, and transient amplifying cells, ...
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Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
Ngày tải lên :
25/10/2012, 11:10
... terminus of GLP-1-A through the linker, which could make the N-terminus of GLP-1-A and the C-terminus of gAdiponectin be free and interact productively with their receptor on target cells In ... SDS-PAGE and Western blot analysis Assay of glucose-lowering effect of gAd-GLP-1-A fusion protein Male KM mice were housed at 23-25°C in a 12-hour light/dark cycle with access to standard powdered ... sonicated and centrifuged to separate the supernatant and inclusion body Part of the production was applied to 12% SDS–PAGE analysis and Western blot analysis (A) SDS-PAGE analysis: Most of the...
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Tài liệu Báo cáo khoa học: A peptide containing a novel FPGN CD40-binding sequence enhances adenoviral infection of murine and human dendritic cells doc
Ngày tải lên :
20/02/2014, 11:20
... that binds to CD40 and enhances adenoviral infection of mouse and human DC and B lineage cells Materials and methods Cell lines Epstein–Barr virus- (EBV-) transformed human B cells were provided ... detectable homology to CD40 ligand (data not shown) and that enhances adenoviral infection of CD40-positive cells of both human and murine origin, including DC and B cells resistant to infection ... FACS analysis; cells were gated as GFP-positive based on fluorescence of uninfected cells, and the percentage of GFP positive cells is shown Error bars represent the standard deviation of triplicate...
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Tài liệu Báo cáo Y học: Presence and regulation of the endocannabinoid system in human dendritic cells potx
Ngày tải lên :
22/02/2014, 07:20
... Purification and quantification of endocannabinoids The extraction, purification and quantification of anandamide, 2-AG and PalEtn from immature and mature dendritic cells requires a set of different ... activation and the maturation of dendritic cells induce a series of events that lead to changes in dendritic cell phenotype and function, we have compared the amount of these compounds in immature dendritic ... glycosylated form of the CB2 receptor protein The dendritic cells band at 45 kDa and the rat spleen at 47 kDa were less intense and are consistent with the previous glycosylated forms of human and rat...
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Báo cáo y học: " High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells." ppsx
Ngày tải lên :
10/08/2014, 10:20
... enhance osteogenesis and vascularization of mesenchymal stem cells Cells Tissues Organs 2009, 190:185-193 Rodbell M: The metabolism of isolated fat cells IV Regulation of release of protein by lipolytic ... potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Pittenger MF, Mosca JD, McIntosh KR: Human mesenchymal stem cells: progenitor cells for cartilage, bone, fat and stroma ... efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells Journal of Biomedical Science 2011 18:59 Submit your next manuscript to BioMed Central and take...
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Báo cáo y học: "Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristic" doc
Ngày tải lên :
14/08/2014, 18:20
... mDCs and moDCs [12] and moDCs and KG-1 cells [13], but no studies have compared the proteomes of all three cell types In this study we compare the proteomes of mDCs, moDCs and KG-1 cells, and ... Figure Phenotypic comparison of cells Phenotypic comparison of cells The expression of cell surface markers on (a) immature and (b) mature mDCs, moDCs and KG1 cells Immature cells were freshly isolated ... expression profiles of cells Cytokine expression profiles of cells Cytokine production by mDCs and the DC models in response to LPS Cytokine concentrations were measured by CBA and analyzed using...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx
Ngày tải lên :
19/02/2014, 02:20
... activity of the human enzyme was not regained upon removal of EDTA and addition of metal-ions Figure shows the hydrophobicity profiles of human and P fluorescens ACMSD, performed according to Kyte and ... [21] From the sequence alignment of human and bacterial ACMSD and the comparison of their three-dimensional structures, the presence of the metallocofactor in the human enzyme can be reliably predicted ... the mean of three independent experiments and are presented as copies of the target variant per 109 copies of 18S RNA Quantitation of ACMSD variants in liver, kidney and brain An isoform-specific...
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Báo cáo khoa học: Ibuprofen binding to secondary sites allosterically modulates the spectroscopic and catalytic properties of human serum heme–albumin doc
Ngày tải lên :
06/03/2014, 01:20
... [20]: koff ẳ koff2ị ẵibuprofen=K2 ẵibuprofenị ỵ koff3ị ẵibuprofen=K3 ẵibuprofenịị ỵ kỵ off 7ị where koff(2) and koff(3) indicate values of koff occurring at K2 < [ibuprofen] < K3, and at ... functional and structural properties of domains I and II [i.e of the 1382 region of (t)HSA] l The values of the Hill coefcients (n1, n2, and n3) for ibuprofen binding to tHSAheme and HSAheme ... low-afnity sites of tHSAheme-Fe(III) and HSAheme-Fe(III) [represented by a and (1 a), respectively, in Eqn (1)] are 0.18 and 0.82, and 0.14 and 0.86, respectively (Table 1) Effect of ibuprofen on peroxynitrite...
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Báo cáo khoa học: A study on genomic distribution and sequence features of human long inverted repeats reveals species-specific intronic inverted repeats pptx
Ngày tải lên :
07/03/2014, 00:20
... the development of the advanced functions of human nervous system in light of the Gene Ontology (GO) profile of human orthologs Results Characters and distribution of LIRs in human autosomes We ... rhesus monkey and mouse orthologs Our data suggest that most of the LIRs shared by human and chimpanzee orthologs were developed and maintained by the common ancestor of humans and chimpanzees ... large number of (CA)n and (GA)n repeats in the human genome, the frequency of their complementary repeats (TG)n and (CT)n is much lower [37] Therefore, the presence of the TG ⁄ CA-rich and TC ⁄ GA-rich...
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Báo cáo khoa học: GTP binding and hydrolysis kinetics of human septin 2 pot
Ngày tải lên :
07/03/2014, 12:20
... Lysates of HeLa cells were electrophoresed through the gel, and then renatured to identify the presence of any kinases capable of using SEPT2 as a substrate In this assay, we found two prominent bands ... properties of SEPT2 for GTPcS and GDP, respectively, may also indicate different conformations in recognizing triphosphate and diphosphate guanine nucleotides The similarity of the Kd, koff and kcat of ... profile of a mixture of 240 pmol of GDP, GTP and GTPcS Absorbance at 254 nm is indicated in arbitrary units 3252 B Fig Guanine nucleotide binding of SEPT2 (A) Equilibrium binding curves of GTPcS...
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Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
Ngày tải lên :
07/03/2014, 15:20
... chromatography and chromatofocusing, as described previously [21] Results Expression and secretion of truncated variants of GCPII To analyze the contribution of individual domains of human GCPII ... Reaction Kit v2.0 (Perkin-Elmer) and an ABI Prism 310 Genetic Analyzer (PE Corporation) Transfection of insect cells and generation of stable cell lines Schneider’s S2 cells (Invitrogen) were maintained ... NaCl and 20 lL of the GCPII sample, were preincubated for 15 at 37 °C in a final volume of 225 lL A 25 lL mixture of 950 nM ÔcoldÕ NAAG (Sigma) and 50 nM H-labelled NAAG (51.9 CiÆmmol)1; New England...
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Báo cáo Y học: Tyrosine sulfation and N-glycosylation of human heparin cofactor II from plasma and recombinant Chinese hamster ovary cells and their effects on heparin binding pot
Ngày tải lên :
08/03/2014, 22:20
... properties of HCII Here, we report on the structure of posttranslational modifications of plasma-derived and recombinant human HCII from CHO cells and their effects on heparin binding MATERIALS AND METHODS ... Amino-acid sequence and tryptic peptides of human HCII The peptides indicated below were detected by MALDI-TOF- and/ or ESI-MS (see Figs and 2, respectively) in tryptic digests of human HCII isolated ... were isolated at random, exposed to 0.05 lM MTX, expanded, and checked for the presence of human HCII mRNA by RT-PCR Positive cell lines were picked and after several rounds of selection with...
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Báo cáo khoa học: Structural and functional characterization of human Iba proteins ppt
Ngày tải lên :
16/03/2014, 06:20
... similarities and differences between Iba1 and Iba2 We investigated Ca2+ binding and homodimerization of Iba1 and Iba2 Furthermore, F-actin binding and cross-linking assays were performed with both human ... composed mainly of a helices (Figs and 2) The core of Iba2 is a pair of EF-hand motifs, denoted as EF-hands and 2, each consisting of two a helices (aA, aB and aC, aD, respectively) anking a ... cross-linking efciency of Iba1 and Iba2 or in the overall morphology of the generated lament bundles Calcium afnity of Iba1 and Iba2 Homodimerization and actin binding of Iba1 and Iba2 were similar...
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Báo cáo khoa học: Multiple-probe analysis of folding and unfolding pathways of human serum albumin docx
Ngày tải lên :
16/03/2014, 16:20
... 400 nm with excitation and emission band pass of nm and nm, respectively ð3Þ DGUÀFH2 O ð4Þ where aF and aU are the intercepts and bF and bU are the slopes of the baselines of the equilibrium fluorescence ... of Nile red Ó FEBS 2004 Mechanism of folding and unfolding of HSA (Eur J Biochem 271) 1795 Fig Equilibrium refolding of HSA in the presence of different concentrations of GdnHCl The recovery of ... values of m and Dm The curve fittings were performed using MICROSOFT ORIGIN software Calculation of the rate constants from the kinetics of refolding HSA was found to follow two phases of refolding,...
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Biochemical and physiological aspects of human nutrition units i & II
Ngày tải lên :
16/03/2014, 18:07
... Storage, and Excretion of Zinc, Copper, and Manganese 746 Selected Functions of Zinc, Copper, and Manganese 752 Assessment of Zinc, Copper, and Manganese Status and Deficiency Symptoms Toxicity of ... Digestion, and Absorption Transport and Conversion of Riboflavin to Coenzymes 469 471 xxii • • • Contents Riboflavin Catabolism and Excretion 472 Functions of Flavocoenzymes in Metabolism 472 Riboflavin ... Assessment of Riboflavin Nutriture 475 Riboflavin Requirements 475 Thiamin Thiamin and Thiamin Coenzyme Structure and Nomenclature Sources, Digestion, and Absorption 476 Transport and Conversion of Thiamin...
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Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx
Ngày tải lên :
17/03/2014, 03:20
... reported for GLUT1 [20] and GLUT3 [21] Comparative analysis of gcGLUT to members of the class I (human GLUT1, GLUT2, GLUT3 and GLUT4), class II (human GLUT5) and class III (human GLUT10) To study ... which contained 20 ng of first strand cDNA, · PCR buffer (20 mM Tris/HCl pH 8.4, 50 mM KCl), 0.2 lM of each primer, 1.5 mM MgCl2 and 0.2 mM of dNTPs PCR Construction and screening of a grass carp genomic ... performed on first-strand cDNAs that were reverse transcribed from total RNA by use of Superscript II reverse transcriptase (Invitrogen) and consisted of 20 ng of first strand cDNA, · PCR buffer...
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Báo cáo khoa học: Substrate specificity and excision kinetics of natural polymorphic variants and phosphomimetic mutants of human 8-oxoguanine-DNA glycosylase pot
Ngày tải lên :
23/03/2014, 05:22
... Time course of excision of 8-oxoGua (d), FapyGua (s) and FapyAde ( ) by OGG1 (B) Time course of excision of 8-oxoGua (d), FapyGua (s) and FapyAde ( ) by Fpg Means and standard deviations of three ... values of kinetic constants for excision of FapyGua and 8-oxoGua by various forms of OGG1 are 500 400 300 200 100 0 10 15 20 Time (min) Fig Excision of 8-oxoGua and FapyGua by wild-type OGG1 and ... values of kcat and Km for FapyGua excision were Table Km, kcat and ksp values for excision of FapyGua and 8-oxoGua from c-irradiated calf thymus DNA by wild-type and mutant OGG1 proteins Mean ± standard...
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