isolation qrt pcr analysis and mir172 mediated cleavage of target genes
báo cáo khoa học: " QTLs and candidate genes for desiccation and abscisic acid content in maize kernels" docx
Ngày tải lên :
12/08/2014, 03:21
... six members of the NCED gene family and two members of the ZEP gene family were identified and mapped by PCR amplification and sequencing Third, expression profiles of the candidate genes during ... physiological traits of interest such as ABA content Colocation between QTLs and candidate genes Colocations between QTLs and candidate genes were based on manual search of the genes related to kernel ... base, and did preliminary molecular analysis for qRT- PCR; CR, performed in silico search of maize ZEPs, primer design and sequencing for confirming in silico analyses, applying to qRT- PCR and mapping...
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Báo cáo khoa học: Genomic structure and expression analysis of the RNase j family ortholog gene in the insect Ceratitis capitata pptx
Ngày tải lên :
23/03/2014, 06:20
... Genomic structure and expression analysis of the Cc RNase Results Expression analysis of Cc RNase In our previous work, we reported the cloning and overexpression of a cDNA sequence of 864 bp (Cc ... Rampias et al Genomic structure and expression analysis of the Cc RNase epidermis and intestine of 6-day-old larvae and adult heads and ovaries, was blotted and hybridized using the Cc RNase cDNA as ... RNase j mRNA of 864 bp has previously been cloned and characterized [17] Northern blot analysis of expression of the RNase j revealed that, in addition to the band at 0.9 kb, a second band at 1.5...
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Báo cáo khoa học: Expression studies of the core+1 protein of the hepatitis C virus 1a in mammalian cells pot
Ngày tải lên :
30/03/2014, 03:20
... in the presence of core was the result of an overloading of the cellular protein-synthesis machinery and of a shortage of its components Finally, we examined the possible effect of core+1 ⁄ S–myc ... of the core+1 ⁄ S protein in a specific and dose-dependent manner, suggesting an effect of the core protein on the translation and ⁄ or the stability of the core+1 protein However, no effect of ... magnified details indicate points of colocalization (B) Fractionation of nuclear and cytoplasmic fractions Separation of cytoplasmic and nuclear fractions from lysates of Huh-7 cells transfected with...
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Expression studies of the proteolytic p10 fragment of the neuronal cdk5 activator in mammalian cells
Ngày tải lên :
05/10/2015, 22:32
... death of Cos-7 cells 56 4.2 Overexpression of p10-cmyc also mediated death of Cos-7 cells 66 4.3 Overexpression of p10-cmyc mediated death of HEK293 cells 73 Chapter 5: Overexpression of p10 ... other hand, the expression of p39 is similar to p35 in 18 adult brains Moreover, the proteolytic pathway and cleavage of p39 is also similar to that of p35 The cleaved products of p39 are p29 and ... syndrome as a result of their concern of having lost something So they wander about and aimlessly search through drawers and closets Some patients rise anxiety for fear of losing track of their caregivers...
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Expression studies of the proteolytic p10 fragment of the neuronal cdk5 activator in mammalian cells
Ngày tải lên :
06/10/2015, 20:35
... death of Cos-7 cells 56 4.2 Overexpression of p10-cmyc also mediated death of Cos-7 cells 66 4.3 Overexpression of p10-cmyc mediated death of HEK293 cells 73 Chapter 5: Overexpression of p10 ... other hand, the expression of p39 is similar to p35 in 18 adult brains Moreover, the proteolytic pathway and cleavage of p39 is also similar to that of p35 The cleaved products of p39 are p29 and ... syndrome as a result of their concern of having lost something So they wander about and aimlessly search through drawers and closets Some patients rise anxiety for fear of losing track of their caregivers...
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báo cáo khoa học: "Structure and expression of the maize (Zea mays L.) SUN-domain protein gene family: evidence for the existence of two divergent classes of SUN proteins in plants" pot
Ngày tải lên :
11/08/2014, 11:21
... members ZmSUN3, ZmSUN4, and ZmSUN5 A summary of the five maize SUN-domain protein genes is provided in Table and the properties and motifs of the CCSD and PM3 subfamilies of these proteins are ... domain (between K364 and V365 in the ORF of ZmSUN1 and between K338 and D339 in the ORF of ZmSUN2; Figure 2A) This ancestral intron position may be a hallmark of this class of SUN genes, as it is ... structures of five maize SUN-domain genes as shown in Table and Figure Three of these genes (ZmSUN1, 2, and 3) are distributed as unlinked loci that map to two different chromosomes; ZmSUN4 and ZmSUN5...
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báo cáo khoa học: " Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha" ppt
Ngày tải lên :
12/08/2014, 03:20
... only two reference genes, BbrizUBCE and BbrizEF1, should be used for qRT- PCR experiments of root, leaf and reproductive tissues of the studied accessions Besides, analysis of ovaries alone should ... ubiquitin-conjugating enzymes and ubiquitin ligases [23] In the qRT- PCR expression profile analysis of suitable reference genes for poplar (Populus trichocarpa ì P deltoides, cottonwood hybrid) and vitis (Vitis ... brizantha qRT- PCR experiments Vegetative and reproductive tissues of apomictic and sexual B brizantha were tested The relative transcription levels of the genes were determined in ovaries and anthers...
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Báo cáo y học: "Characterization of N200 and P300: Selected Studies of the Event-Related Potential Salil H. Patel 1 and Pierre N. Azzam 2"
Ngày tải lên :
02/11/2012, 11:08
... relationship of age, gender, handedness, and sidedness to the size of the corpus callosum Academic Radiology 1994; 1:243-248 69 Driesen NR, Raz N The influence of sex, age, and handedness on ... infrequently-appearing stimulus of with a two-stimulus oddball task, regardless of attentional (i.e., target or nontarget) status [38] The P3a has also been demonstrated experimentally in target/ nontarget tasks ... between age and callosal size Conversely, a subsequent review of MRI and post-mortem reports indicated a callossal volume relationship to handedness and gender [69]; the clinical significance of these...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
Ngày tải lên :
15/02/2014, 01:20
... characterization of numerous complexes of the human HPRT and several bacterial and protozoan HPRTs have been undertaken [13–17] The structure of human HPRT can be divided into two domains: a core domain and ... domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig 1A) The two subunits ... the base of bound GMP is stacked between Val143 and Phe194 N1, N2 and O6 of the nucleotide form hydrogen bonds to main chain atoms, whereas the side chain of Lys173 interacts with both O6 and N7...
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Tài liệu Báo cáo khoa học: Mechanism of dihydroneopterin aldolase NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes docx
Ngày tải lên :
19/02/2014, 00:20
... 35, and 70 after the addition of the enzyme The top spectrum is that of DHMP for comparison Only the NMR signals of the 2¢ and 3¢ protons of DHNP and DHMP are shown The chemical structures of ... the measurements of both equilibrium and kinetic constants of the physical steps by equilibrium and stopped-flow fluorimetric analysis and the determination of the rate constants of the chemical ... NP and MP and 2248 between HP and HPO in accordance with the similar shapes and sizes between NP and MP and between HP and HPO The different Kd values are proportional to the different values of...
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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
Ngày tải lên :
21/02/2014, 15:20
... of hydrogen, modification of bonds, potentials of forcefield, and fixation of heavy atom, backbone, and Ca, were performed for molecular mechanics calculations and then the energy minimization of ... possible dimeric structures of LPL, the head-tohead and the head-to-tail models, the studies of the chimeric proteins of hepatic lipase and LPL and the tandem repeat approach of LPL [21,34] support ... of primary-sequence similarity with pancreatic lipase, and the conservation of most of the disulfide bonds between LPL and PL suggests a similar tertiary structure For the two dimeric models of...
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Tài liệu Euphorion Being Studies of the Antique and the Mediaeval in the Renaissance - Vol. II pot
Ngày tải lên :
21/02/2014, 21:20
... piles of cabbage and cauli-flowers, the rows of tin ware and copper saucepans, the heaps of maccaroni and pastes, of spices and drugs; the garlands of onions and red peppers and piles of apples; ... of the spectator, than the harmony of villainies, of various combinations of black and hog-like bestiality, and fox and wolf-like cunning and ferocity with wicked human thought and self-command, ... also, by the hand of two of her greatest poets, has given us a picture And first, of Roland Of him, of Count Orlando, we are told by Messer Lodovico Ariosto, that in consequence of his having...
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Báo cáo khoa học: Fluorescence studies of the replication initiator protein RepA in complex with operator and iteron sequences and free in solution pdf
Ngày tải lên :
07/03/2014, 04:20
... global and local A C 5398 dynamics of the AEDANS and W94 probes in free and DNA-bound RepA The decay of the total uorescence intensity was recorded, as well as the decays of its vertically and horizontally ... of the acceptor It is independent of FRET and depends only on the acceptor concentration The extinction coefcients of W94 [25] and AEDANS [26] are assumed constant as a function of labeling and ... of free and bound unlabeled C160RepA and wild-type RepA, with the protein concentration constant The ratio of the apparent FW94 of bound and free RepA was then used to estimate the fraction of...
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Báo cáo khoa học: Cloning and expression of the first nonmammalian interleukin-11 gene in rainbow trout Oncorhynchus mykiss pdf
Ngày tải lên :
07/03/2014, 16:20
... profile of bacterial-challenged rainbow trout was surveyed by means of suppression subtraction hybridization and sequence analysis [22] This resulted in identification of a number of immune genes, ... poly(I:C) (50 lgÆmL)1) for 3, and 24 h or IL-1b (25 ngÆmL)1) for 0.5–8 h, and total RNA prepared for RT PCR analysis The cycle numbers for PCR were 21 and 30 for b-actin and IL-11, respectively Neg ... of this clone was highly induced in tissues of bacterial infected fish, a full-length cDNA clone as well as a genomic DNA clone were isolated and sequenced, and the expression and modulation of...
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Báo cáo khoa học: Differential expression pattern of the novel serine⁄threonine kinase, STK33, in mice and men doc
Ngày tải lên :
16/03/2014, 23:20
... experiments: mice void of Camk4, as a consequence of disrupted promoter structure and exons I and II, exhibit impaired follicular development and ovulation [25] and impaired spermiogenesis in males ... band corresponds to the E coli native protein biotin carboxyl carrier protein (BCCP) Track in (B) corresponds to the purge of the column and shows a trace of antigen Track and contain 572 ng and ... causing a partial mixing of venal and arterial blood and a decrease of blood flow to the lungs On the other hand, data from the source database [34] suggests a higher expression of STK33 in neuroblastoma...
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Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx
Ngày tải lên :
17/03/2014, 03:20
... first strand cDNA, · PCR buffer (20 mM Tris/HCl pH 8.4, 50 mM KCl), lM of each primer, 0.2 mM of dNTPs, 1.5 mM MgCl2 and U of Taq DNA polymerase (Invitrogen) The PCR program consisted of predenaturation ... Sequence analysis showed that both GT-cDNA1 and GT-cDNA2 contain a 5¢-UTR of 203 bp and a coding region of 1599 bp (excluding the stop codon), but the 3¢-UTR of GT-cDNA1 is 345 bp while that of GTcDNA2 ... each primer, 1.5 mM MgCl2 and 0.2 mM of dNTPs PCR Construction and screening of a grass carp genomic DNA library Genomic DNA was extracted from grass carp liver by the use of Genomic-tips (Qiagen)...
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Báo cáo khoa học: Antimicrobial and conformational studies of the active and inactive analogues of the protegrin-1 peptide docx
Ngày tải lên :
22/03/2014, 21:20
... In IB-367 and BM-1, the presence of strong daN(i,i + 1) and weak (or absence of) dMN(i,i + 1) and dbN(i,i + 1) NOE connectivities in the Gly3–Arg8 and Cys14– Gly16 regions of IB-367 and in the ... activity of the peptides A better understanding of the mode of action of these peptides is crucial for the development of a new generation of antibiotics It is known that, in the absence of both ... B, Epand RM & Gellman SH (2006) Roles of salt and conformation in the biological and physicochemical behavior of protegrin-1 and designed analogues: correlation of antimicrobial, hemolytic, and...
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Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx
Ngày tải lên :
23/03/2014, 13:20
... h, 22 °C) and harvested by centrifugation (yield 25 g, wet weight) Isolation and purification of CPS and LPS The cells were washed with 2.5% saline (w/v) and extracted by the method of Westphal ... corroborated by NMR and MS analyses performed on CPS and carboxyl-reduced LPS Amino acid analysis confirmed the presence of L-Ala in both polysaccharides Fatty acid analysis of both phenol- and aqueous-phase ... acid The sequence of constituent glycoses and the location of L-Ala were confirmed by 2D NMR analysis performed on both CPS and aqueous-phase LPS and their methanolysis products, and CE-ES-MS methods...
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Báo cáo Y học: Engineering and mechanistic studies of the Arabidopsis FAE1 b-ketoacyl-CoA synthase, FAE1 KCS pot
Ngày tải lên :
24/03/2014, 03:21
... Lane and 9, respectively, P and S fractions of 0.5% Triton X-100 plus 0.32 M NaCl treatment of thrombin digested microsomes Lane 10 and 11, respectively, P and S fractions of M NaCl treatment of ... respectively Lanes and 5, respectively, P and S fractions of untreated thrombin digested microsomes Lanes and 7, respectively, P and S fractions of 0.5% Triton X-100 treatment of thrombin digested ... decarboxylation, and substrate specificity Additionally, this provided an opportunity to analyze the effect of mutagenesis of His391 and Asn424 in FAE1 KCS To our knowledge, this is the first report of the analysis...
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Báo cáo khoa học: Characterization and expression analysis of the aspartic protease gene family of Cynara cardunculus L. docx
Ngày tải lên :
30/03/2014, 09:20
... CA-5¢ Probe CA-5¢ -and 5¢-RACE PCR PCR 5¢ Primer extension RT -PCR (cardosin A) RT -PCR (cardosin A) RT -PCR (cardosin C) RT -PCR (cardosin C) RT -PCR (cardosin D) RT -PCR (cardosin D) RT -PCR (cardosin ... bp and ) 74 bp of the cardosin A and D genes, respectively The 5¢-flanking regions of the cardosin A, C and D genes share a high degree of similarity (Fig 3) However, the respective region of ... of cardosin A, B and D genomic clones with the respective cDNAs (Fig 2) revealed the presence of an intron in the 5¢-UTR of the genes The nucleotide sequences of the cDNA and genomic clones of...
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