isolation purification and identification of active

... Inhibition of protein glycation 75 3.8 Purification and identification of active ingredient from extract WN 76 3.9 Low pro-oxidant effect of YCT extract 79 3.10 Inhibition of PR bleaching and 3-nitrotyrosine ... Non-enzymatic protein glycation 47 2.2.10 Inhibition of hypochlorous acid-induced DNA damage 47 2.2.11 Isolation, purification, and identification of active ingredient from Cratoxylum cochinchinense ... Whiteman, M., Peng, Z F., Jenner, A., Yong, E L., and Halliwell, B (2004) Characterization of antioxidant and antiglycation properties and isolation of active ingredients from traditional chinese medicines...

Ngày tải lên: 16/09/2015, 15:53

... Structures and molecular weights of soyasapogenol B, C and B1 98 Fig 28 LC-MS Chromatogram of standard and purified sample of soyasapogenol B Panels (A and C) are authentic standard and samples of ... 79 6.2.1.5 HPLC Analysis of Soyasaponins and Soy isoflavones 79 6.2.1.6 LC/MS Analysis of Total soyasaponins and Soy isoflavones 79 6.2.2 Comparison of Soyasaponin Concentration Methods ... authentic saponin identification and quantification The purification process is complex and laborious with long sample preparation and extraction times and yielding relatively low amounts of soyasaponins...

Ngày tải lên: 11/09/2015, 10:06

... Switzerland) Once suspicious colonies are identified, confirmation of the isolates as E coli O157:H7 is dependent upon biochemical identification and demonstration of the presence of somatic and ... strains), C1-6 (strains of Cornell Univ.) and P1-7 (strains from E coli reference center of Pennsylvania State Univ.) b The presence of Stx1 and Stx2 “-” and “+” indicate negative and positive, respectively ... assessment of the risks difficult, and the options for management and control unclear The objectives of this study are (i) to examine the prevalence of E coli O157:H7 in slaughterhouses and retail...

Ngày tải lên: 07/08/2014, 18:21

... indicates the cytoblast of an infected FCWF cell The black arrow indicates the inclusion bodies of GPV in the cytoplasm of cells Scale bars = 200 nm Isolation and identification of a canine coronavirus ... logarithm of the highest dilution of serum that could neutralize 100 TCID50 of the virus Total RNA was extracted from the giant pandas’ spleens, infected and non-infected FCWF cells (as positive and ... Bacteriology failed to grow organisms in cultures inoculated with liver and spleen after 48 h culture Fig Isolation and culture of giant panda virus (GPV) in Felis catus whole fetus (FCWF) cell line (A)...

Ngày tải lên: 07/08/2014, 23:22

... activity of the nhexane and methanol extracts of A paniculata Seven compounds, namely andrographolide, bis-andrographolide 14-deoxy-11,12-didehydroandrographolide, andrograpanin, 14-deoxyandrographolide, ... compounds including andrographolide enhance proliferation and IL-2 secretion in hPBL [41] Andrographolide enhances secretion of IL-2 and IFNγ by T cells and stimulates the production of cytotoxic T ... terms of bioactive properties and abundance Among the andrographolide analogues, 14-deoxy-11,12-didehydroandrographolide is immunostimulatory, anti-infective and anti-atherosclerotic; neoandrographolide...

Ngày tải lên: 13/08/2014, 15:21

... Representative HPLC profiles for purified compounds to and and fractions to 74 3.4.1 Structures of sesquiterpene lactones 75 3.4.2 Skeletal structures of flavonoids, isoflavonoids and neoflavonoids 76 ... Discussion and conclusions Chapter Isolation and characterization of components from ethyl acetate fraction of Ixeris sonchifolia Hance 48 55 3.1 Introduction 56 3.2 Materials and methods 56 3.2.1 Isolation ... observe, feel and understand the world I’m writing this thesis to share with all of them I Table of contents Acknowledgements I Table of contents II Abstract VII List of tables X List of figures...

Ngày tải lên: 11/09/2015, 10:06

... aquaculture and especially in environmental treatment It helps disintegrate dreg, mud In this study Isolation selection and identification of Bacillus subtilis from “mud-dreg of beer” 21 strains of bacteria ... 16S-9F and 16S-1525R The result of PCR perform size band of strains and Bacillus subtilis control had 1500bp Sequencing perform that strains (Bs4 and BN5) were Bacillus subtilis (99% and 98% ... waste water of beer and beer wallows in the brewing process Objective: Isolation, selection and identification of Bacillus subtilis from beer dregs, that is done with the aim of isolating, identifying...

Ngày tải lên: 06/10/2015, 12:58

... ethidium bromide and UV irradiation for detection of DNA in gels, thereby preventing DNA damage of the long molecules and enhancing isolation of full-length clones The range of cloning kits and vectors ... ordering process and the cost of synthesis is relatively cheap An example of appropriate design of primers to add attB1 and B2 sites to the 5′ and 3′ ends respectively of a gene of interest are ... N-glycosidic bonds between uracil and deoxyribose leading to a loss of the base The loss of bases leads to disruption of base pairing and therefore exposure of single strands that can be annealed to...

Ngày tải lên: 25/10/2013, 22:20

... implications of the cycle of histone acetylation and deacetylation that accompanies cycles of transcription, and highlight the special significance of histone H4 lysine 16 acetylation Active chromatin and ... somewhat of an over-simplification, as some active genes reside within heterochromatin Drosophila polytene chromosomes offer one of the clearest examples of active chromatin domains whereby active ... simplest level, loss of histone H1 is sufficient to reduce the level of compaction of the chromatin fibre, and at active genes the amount of histone H1 is reduced compared with inactive genes [95–97]...

Ngày tải lên: 14/02/2014, 18:20

... properties The cause of these differences is not known Km and kcat measurements of hevamine and mutants Comparison of the steady-state kinetic parameters of hevamine and the Tyr183Phe and Asp125Asn ... pH 5.0 and above At pH 8.0 and above, there is no activity remaining An Ó FEBS 2002 Active site mutants of hevamine (Eur J Biochem 269) 897 Table Relative lysozyme activity of hevamine and hevamine ... observations Number of unique reflections Completeness (%)

Ngày tải lên: 22/02/2014, 04:20

... physical meaning of the spectral pressure modes as well as the linkage between the pressure modes and hidden events of the unsteady pressure fields Synthesis and identification of random pressure ... ratios of B/D=1 and B/D=5 in the wind tunnel tests Proper orthogonal decomposition 2.1 Definition The POD is optimum approximation of random field The main idea of the POD is to find out a set of ... orthogonal basic vectors which can expand a multi-variate random process into a sum of products of these basic orthogonal vectors and single-variant uncorrelated random processes Let consider the...

Ngày tải lên: 05/03/2014, 14:20

... MALDI-TOF/TOF MS and LC-ESI-Q-TOF MS This "shotgun" strategy resulted in a lower number of total spectra and identified a lower number of peptides, but yielded a comparable number of protein identifications ... 115–122) of the L3L protein Method 5: MALDI-TOF/TOF MS Direct trypsin digests of the membrane- and core-enriched fractions were also analyzed using MALDI-TOF/TOF MS to take advantage of complementary ... desorption ionization tandem mass spectrometer with time -of- flight/time -of- flight optics (MALDI-TOF/TOF), 2.) a quadrupole-time of flight mass spectrometer (LC-ESI-Q-TOF), and 3.) a quadrupole...

Ngày tải lên: 19/06/2014, 08:20

... MALDI-TOF/TOF MS and LC-ESI-Q-TOF MS This "shotgun" strategy resulted in a lower number of total spectra and identified a lower number of peptides, but yielded a comparable number of protein identifications ... 115–122) of the L3L protein Method 5: MALDI-TOF/TOF MS Direct trypsin digests of the membrane- and core-enriched fractions were also analyzed using MALDI-TOF/TOF MS to take advantage of complementary ... desorption ionization tandem mass spectrometer with time -of- flight/time -of- flight optics (MALDI-TOF/TOF), 2.) a quadrupole-time of flight mass spectrometer (LC-ESI-Q-TOF), and 3.) a quadrupole...

Ngày tải lên: 20/06/2014, 04:20

... and only minimal presence of Muc16 on the cell surface (Figs 6a–d) Similar results were obtained with both the 618F and 653F antibodies (Fig 6c and 6d) Discussion Figure ting Identification of ... SDS-PAGE and probed with a panel of anti-MUC16 monoclonal antibodies Arrows indicate migration of 250 kDa molecular weight marker and identity of antibody used is shown on the right of each blot ... Specificity of 618F for human MUC16 Specificity of 618F for human MUC16 (A) Purity of human MUC16 isolated from spent media of OVCAR-3 cells as determined by Coomassie Blue (1) and silver staining (2) of...

Ngày tải lên: 20/06/2014, 07:20

... thankful to Professor Les Copeland (Faculty of Agriculture, Food and Natural Resources - The University of Sydney, New South Wales, Australia) and Professor Kaeko Kamei (Department of Biomolecular ... proteins Generally, the two-step chromatography of AEX and HIC was sufficient for the purification of FIII-1, FIII-2, and FII, since their SEC and SDS-PAGE profiles represented pure proteins Fraction ... alignment of fragments obtained from MS/MS analyses of FIII-1 and FIII-2 from P excavatus with lumbrokinase and its precursor from L rubellus (sp:P83298 and U25647) and E fetida (gpu:EU167737 and AY438624),...

Ngày tải lên: 20/06/2014, 23:20

... citatsomreht a ot detcennoc )nedewS ,aicamrahP( tinu IIrohpitluM eht ni demrofrep saw sisylana citerohportcele ehT la te gnisucof cirtceleosi lacitylanA snoitacifidom thgils htiw ]42[ nibwoT fo dohtem ... ,BKL( rettolb yrd-imes tolBavoN a gnisu demrofrep saw sisylana tolbonummI la te gnittolbonummI ]31[ ilmmeaL yb debircsed snoitidnoc eht ot gnidrocca demrofrep saw siserohportcele dna ,selpmas ,sleg ... noitaraperP )ASU ,daRoiB( lleC balS-lauD II naetorP -iniM eht ni demrofrep saw sisylana citerohportcelE EGAP-SDS ]3[ dohtem drofdarB eht yb detamitse saw sPLG eht fo tnetnoc nietorp ehT fo sPLG...

Ngày tải lên: 07/08/2014, 20:23

... is the number of non-seed genes above the cutoff, True negatives is the number of non-seed genes below the cutoff, and False negatives is the number of seed genes below the cutoff Random prediction ... molecular mechanisms of human diseases, and can in turn guide the development of relevant therapy as illustrated in the case of Alzheimer's disease and hypercholesterolemia Materials and methods Human ... non-seed genes above the cutoff, TN (true negative) is the number of non-seed genes below the cutoff, and FN (false negative) is the number of seed genes below the cutoff Finally, an AUC is calculated...

Ngày tải lên: 09/08/2014, 20:20

... 45 and 30 in the second and third rounds individually, and increasing washing with TBST for times and times in the second and third round individually Sequence Analysis of Selected Phages and ... ratio of phages increased about 100 fold A group of peptides capable of binding specifically to the renal carcinoma cells were obtained, and the affinity of these peptides to the targeting cells and ... controls Immunofluorescence Microscopy and Image Analysis Immunofluorescence microscopy was used to study the affinity of synthetic peptide (ZT-2) binding to A498 and renal carcinoma A498 and HK-2...

Ngày tải lên: 10/08/2014, 10:21

... Journal 2010, 7:241 http://www.virologyj.com/content/7/1/241 Page of Figure Expression and purification of the tgK protein M represented standard protein molecular weight markers The arrow marked the ... http://www.virologyj.com/content/7/1/241 Page of Figure Purification of the rabbit anti-tgK polyclonal antibody and Western blot assay M represented standard protein molecular weight markers; M1 ... http://www.virologyj.com/content/7/1/241 Page of Figure Indirect immunofluorescent microscopy was used to monitor the DEV antigen distribution in liver, harder’s glands, cecum, spleen and kidney of the infected ducks...

Ngày tải lên: 12/08/2014, 01:21