isolation of human oral keratinocytes and their use in experimental systems

... presence of saturating concentrations of the metal cofactor Together, these data are indicative of two modes of substrate binding (i.e open and closed) The closed, active form of UDP-Glc binding is ... hydrolysis of UDP-Glc in the absence of toxin Inhibition studies were performed by varying the MUG and UDP-Glc concentrations and following the kinetics of the hydrolysis reaction Synthesis of MUG, ... 7.6 and 150 mM KCl containing varying concentrations of UDP-Glc (0–145 mM) were studied The variable concentrations of UDP-Glc were obtained by starting with a stock solution containing all of...

Ngày tải lên: 08/03/2014, 22:20

... that interesting to use, due to the fact that they are of the dominant type, the RAPDs and AFLPs have a great advantage in terms of ease of use in the laboratory Indeed, fingerprint types of patterns ... to in Tables I and II are still in use and the choice of one or another can be guided by the variety of parameters indicated, mainly according to the goal of the study and the importance of the ... available, indications of high densities of SNPs in defined regions can be found A sequencing study of fragments of the leptin and amyloid precursor protein (APP) genes in 22 diverse individuals...

Ngày tải lên: 09/08/2014, 18:21

... selection of aptamers with both fast and slow binding kinetics It also can be used to accurately determine the binding affinities, kinetics and specificity of aptamer sequences In my PhD, the use of ... 1-4 using both the NECEEM and fluorescence intensity methods 80 Table 4.4 Binding affinities of lysozyme, trypsinogen, chymotrypsinogen A and myoglobin using fluorescence intensity against ... the use aptamers as affinity probes in ACE include IgE, HIV type reverse transcriptase, thrombin and antithrombin III12-14 The use of aptamers in biosensors is becoming a huge area of interest in...

Ngày tải lên: 10/09/2015, 09:22

... complexation of Cd to polyphosphate granules in the cell membrane and intracellular Cd binding proteins in a tolerant strain of Pseudomonas putida, grown in a defined medium containing mM Cd2q ... on membrane structure and function by binding to the ligands such as phosphate and the cysteinyl and histidyl groups of proteins (Collins and Stotzky, 1989) The effect of Cd on the soil enzymes ... ecophysiological indicator of heavy metal stress in soil (Anderson and Domsch, 1990; Brookes, 1995) qCO2 decreased in the heavily Cd contaminated soil over that in control during the first 2-d incubation (Landi...

Ngày tải lên: 15/03/2014, 23:21

... translocation depending upon the biophysical properties of CPPs and their cargo [7,14,24] Interestingly, most investigations involving CPPs have been carried out in mammalian cell lines and there are ... plant tissues of triticale seedlings [27] Macropinocytosis dependent transduction of fluorescent proteins by arginine-rich intracellular delivery (AID) and Tat-protein transduction domain has been ... Queen in Right of Canada, as represented by the Minister of Agriculture and Agri-Food Canada Journal compilation ª 2008 FEBS A Chugh and F Eudes CPPs and their cargo in wheat Effect of inhibitors...

Ngày tải lên: 23/03/2014, 07:20

... form a network of proteins involved in the regulation of phospholipid metabolism Materials and methods Strains and culture conditions Yeast strains used in this study are listed in Table Cells ... integrative versions of C-terminal yEGFP fusions in frame with the ORF of interest Thus, yEGFP fusions were present in single copy in the genome and driven by the native promoter of the ORF of ... formation, indicating that Sec14p and the two homologues act as inhibitors of Plb1p in vivo This observation explains at least in part the low Plb1p activity in wild-type cells Interestingly, the inhibitory...

Ngày tải lên: 23/03/2014, 18:20

... 05,/ Case Non-inductive line of Non-inductive load of Case Non-inductive line of Inductive load of Case Inductive line of Inductive load of Case Inductive line of Non-inductive load of 1 — j — lj ... space, and of which the complex quantity in a plane is only a special case M B STKINIHTTZ: In introducing j , first as distinguishing index and then defining it as V — my object was to introduce ... phase of the main current and the E M F In consequence hereof, the current will change in the line •from point to point, in intensity as well as phase, and the E M ÆTHE ORCE RF I 58 8TEINMETZ...

Ngày tải lên: 04/06/2014, 12:33

... a kind of land use described or defined in a higher degree of detail than that of a major kind of land use (such as rainfed agriculture or forestry), as an abstraction of actual land -use systems ... ; examples of improvements in land quality (e.g., results of soil conservation and land -use planning case studies); Land quality indicators and their use in sustainable agriculture and rural development ... indicators and their use in sustainable agriculture and rural development 19 Land quality indicators and their use in sustainable agriculture and rural development 19 The context of indicators in FAO...

Ngày tải lên: 06/07/2014, 08:20

... difference in the world [in gaining buy -in and involvement].’ – Researcher, RSS Ongoing education and training opportunities about the issue and approaches to addressing it, capitalizing on existing ... for advancing research use activities: Their [management] contributions and support would have been in the way of providing staff time to go to meeting and providing openings within their departmental ... KT and evidence-based decision-making and in understanding and improving capacity for research use within health systems (i.e., federal health departments, provincial or state departments of...

Ngày tải lên: 10/08/2014, 10:23

Ngày tải lên: 13/11/2014, 09:43

... curves showing: (a) the effect of varying IED Reduction in IED causes increase in C and hence the VTH is reduced; (b) the effect of increasing the dielectric constant, ε of the ambient using liquid ... vii in obtaining GNRs by unzipping carbon nanotubes has made the prospect of fabricating GNR-based electronic devices in large quantities more promising and hence, detailed understanding of the ... characteristics of the floating gate design with (red, thick lines) and without (black, thin lines) the added oxide layer for channel length of (c) 10.1 and (d) 14.9 nm The solid and dash lines indicate increasing...

Ngày tải lên: 10/09/2015, 08:39

... Lai Lai and Dr Yu Hao, for their help and encouragement during my staying in Singapore Their presence has created an enjoyable and productive working environment and made my staying in Singapore ... evidence showing that polyamines (PAs), including putrescine (Put), spermidine (Spd) and spermine (Spm), and its oxidative product, H2O2, are implicated in shoot morphogenesis in vitro and/ or somatic ... in siliques and rosette leaves, lower in flower and cauline leaves and lowest in stem and root (Soyka and Heyer, 1999) Unlike ADC1, ADC2 transcript was accumulated predominantly in siliques and...

Ngày tải lên: 12/09/2015, 10:19

Ngày tải lên: 16/09/2015, 17:11

... contours and streamlines for flow past a pair of tandem cylinders (L=2.5D) at Re=200 in a circle ……………………165 Figure 6.16 Drag and lift coefficients of flow past a pair of tandem cylinders (L=2.5D) ... the saving of time and human- labor on the mesh construction when complex geometry is involved Instead of mesh generation, mesh-free methods use node generation From the point of view of computational ... performance of EFGM is only minimally affected by irregular placement of nodes One of the main drawbacks of the method is the additional cost of building the moving least square functions An increase in...

Ngày tải lên: 16/09/2015, 17:12

... everyone and everything appeared in my past three years Firstly, I need to thank all my families in China for everything they did in the past 27 years They built my world with their hands, their ... Screening Results of the Model Reaction Table 2.3 Preliminary Screening of Solvents and Fluoride salts Table 2.4 Screening of Fluoride Salts with the Model Reaction Table 2.5 Screening of Different ... reactivities of both Q+R- and Q+OH- (in Starks pathway) are increased due to larger separation of charges and reduced number of hydration, which results in higher reaction rate than that of homogeneous...

Ngày tải lên: 30/09/2015, 10:12

... and thrombomodulin protein markers in the BALF and protein leakage into the airspaces We show here that oral administration of a low dose of doxycycline, a MMP inhibitor which inhibits gelatinases ... pro-domain (Pro), which contains a zinc ligating thiol (–SH) group, and catalytic domain, which contains a zinc ion (Zn2+) Hemopexin-like domains are essential in binding to tissue inhibitors of metalloproteinases ... factor (TNF) and interleukin-1β (IL-1β) into their active form, both being involved in inflammatory processes In the modulation of cell death and cell migration, MMPs cleave the Fas ligand and enable...

Ngày tải lên: 13/10/2015, 16:41

... cisplatin forms two kinds of cross-links with DNA: intrastrand and interstrand Platinum complexes react with cellular DNA, primarily binding to the N7 positions of guanine bases, forming 60–65% ... Platinum cross-linking of adenines and guanines on the quadruplex structures of the AG3(T2AG3)3 In uence of platinum complexes on G-quadruplexes and (T2AG3)4 human telomere sequences in Na+ and ... stability of the G-quadruplex containing platinable guanine in the connective loop To date, determination of the G-quadruplex folding in the presence of platinum derivatives in K+ solution remains...

Ngày tải lên: 23/03/2014, 06:20

... frequently used means of transport, particularly in rural areas However, those rules, although not prohibiting imports of trailers and their marketing in Italy, have the effect of limiting their use ... application of principles to facts will involve a careful examination of the proportionality of the measure, particularly in the case of measures governing the use of products In relation to heading ... respect the principles of non-discrimination and of mutual recognition of products lawfully manufactured and marketed in other Member States, as well as the principle of ensuring free access of Community...

Ngày tải lên: 30/03/2014, 06:20

... 100°C for 10 in a 10-fold volume of water to inactivate intrinsic proteinases After cooling to 4°C, CH3COOH and HCL were added at final concentrations of M and 20 mM, respectively Following homogenization ... the causes of small solitary peripheral nodules The final diagnoses of the controls consisted of the healing stage of pulmonary suppuration in case and lung nodule of unidentified etiology in cases ... size of the clear zone surrounding the wells containing defensins, penicillin G, and those containing control sample Peptide synthesis The reduced peptide of hBD-4, designed by García et al and...

Ngày tải lên: 12/08/2014, 18:20

... HNF assisted in co-ordination of the study, and participated in writing and revising of the manuscript RSF participated in patient enrolment, and participated in writing and revising of the manuscript ... participated in patient enrolment, and participated in writing and revising of the manuscript AGD provided statistical analysis of results and interpretation, and participated in the writing and revising ... Cost-effectiveness of recombinant human activated protein C and the influence of severity of illness in the treatment of patients with severe sepsis J Crit Care 2003, 18:181-191 Quartin AA, Schein RM, Kett...

Ngày tải lên: 13/08/2014, 10:20