isolation northern blotting and qrt pcr
Báo cáo y học: "Matrin 3 is a co-factor for HIV-1 Rev in regulating post-transcriptional viral gene expressio" pptx
Ngày tải lên :
13/08/2014, 01:21
... phosphatase-conjugated secondary antibody and detected using a chemiluminescence substrate (Applied Biosystems) RNA isolation, Northern blotting and qRT- PCR Total RNA from cells was extracted with ... monoclonal Matrin 3, (Abcam) and rabbit anti-GFP and anti-HA (Cell Sciences) are commercially available Western Blotting, and Immunoprecipitation Western blotting and immunoprecipitation were ... wild type and mutant Matrin together with pCMV Gag-Pol RRE, as indicated; and 24 hours later, cells were harvested and cell lysates were analyzed by Western blotting Figure 1B (lanes and 2) shows...
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Báo cáo khoa học: Isolation, characterization, sequencing and crystal structure of charybdin, a type 1 ribosome-inactivating protein from Charybdis maritima agg. potx
Ngày tải lên :
16/03/2014, 14:20
... N-terminal domain is composed of a six-stranded b-sheet, which in turn contains four antiparallel central b-strands (4–7, Fig 4) and two parallel outer b-strands (1 and 8, Fig 4) The b-sheet is attached ... be in the region of the gap between Ser98 and Gly102 In the N-terminal domain, there is also an additional two-stranded b-sheet (strands and 3, Figs and 5), which lies opposite the C-terminal ... 5), which is less conserved among the RIPs, and a two-stranded b-sheet and 10 (Figs and 5) In charybdin there exists an additional 310 helix (J, Figs and close to the C-terminus of the protein)...
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SUCCESS FACTORS OF PLACE MARKETING: A STUDY OF PLACE MARKETING PRACTICES IN NORTHERN EUROPE AND THE UNITED STATES doc
Ngày tải lên :
23/03/2014, 04:21
... brand identity, brand position and brand image Brand Identity How the owners want the brand to be perceived Brand Image Brand Positions How the brand is perceived in reality The part of the brand ... 2000) Brands are the basis for long-term success for numerous firms and organisations (See also Rein et al 198726) Building strong brands requires clear brand identity and brand position, and also ... in Europe has led to an almost random handling of place-buyers, and, consequently, almost random results, as few places in Europe actually anticipate and understand the resulting decision process...
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Báo cáo Y học: Isolation, enzymatic properties, and mode of action of an exo-1,3-b-glucanase from Trichoderma viride doc
Ngày tải lên :
24/03/2014, 04:21
... b-oligoglucosides was analyzed by TLC and 1H and 13C NMR spectroscopy as described below Published values for 1H and 13C chemical shifts of b-oligosaccharides with different DP and linkage types [26,28–30] ... definitions, see footnotes): G4G4G3G and G4G3G were produced by digestion of barley glucan with a 1,3-1,4-bglucanase and purified [26] The G3G3G3G3G3G, G3G3G3G3G, and G3G3G3G were prepared by formic ... procedure using BSA as a standard [32] 1H-NMR spectra and 13C-NMR spectra were recorded with an AMX-500 Bruker spectrometer Prior to NMR analysis laminarin, buffer components, and the enzyme were freeze-dried...
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Population structure attributable to reproductive time: isolation by time and adaptation by time docx
Ngày tải lên :
28/03/2014, 16:20
... Molecular Ecology, 14, 901–916 904 A P H E N D R Y and T D A Y Fig Illustrations of dispersal under isolation by distance (IBD) (panel A) and isolation by time (IBT) in the case of asexual reproduction ... first individual) and reproductive lifespan (the length of time from the start of breeding by an individual until its death) Data are for female (lines and 2) and male (lines and 4) sockeye salmon ... and anonymous reviewers Additional advice on relevant literature was provided by J McKay, L Rieseberg, L Rowe, R Shaw, and B Sinervo A Hendry and T Day are supported by the Natural Sciences and...
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Báo cáo lâm nghiệp:"Aboveground biomass relationships for beech (Fagus moesiaca Cz.) trees in Vermio Mountain, Northern Greece, and generalised equations for Fagus sp." ppt
Ngày tải lên :
08/08/2014, 01:21
... biomass values vary with species, stand age, site quality, climate, and stocking density of stands [1, 4, 11] Implementing allometric equations beyond the specific site and the diameter range for which ... Balkan region and particularly Northern Greece, is the contact zone of the European Beech (F sylvatica subsp sylvatica L.) and Oriental beech (F sylvatica subsp orientalis Lipsky) Strid and Tan [28] ... the correction factor and SEE = ˆ å( ln Yi – ln Yi ) ¤ ( n – p ) is the standard error of the estimate of the regression; n and p denote the number of the observations and the fitted parameters,...
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Báo cáo y học: "Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries" pps
Ngày tải lên :
09/08/2014, 22:23
... heating and cooling rates (6° C/s and 4.5°C/s, respectively) on thermocycler (see Materials and methods for make and model) Running the PCR protocol on thermocyler (at its default heating and cooling ... of PCR- amplified and PCR- free Illumina libraries Kozarewa et al [21] developed a protocol for Illumina sequencing without PCR to amplify and enrich adapterligated DNA fragments We sequenced a PCR- amplified ... to a double-stranded size marker qPCR Primer pairs for ‘PER’ and human qPCR assays, their genome of origin, sequence, length and GC-content of amplicons are listed in Tables S1 and S2 in Additional...
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Báo cáo y học: "Genetic analysis of HIV-1 Circulating Recombinant Form 02_AG, B and C subtype-specific envelope sequences from Northern India and their predicted co-receptor usage" pot
Ngày tải lên :
10/08/2014, 05:21
... sequences from HIV-1 infected individuals from Northern India and report the presence of HIV-1 CRF02_AG for the first time Methods Genomic DNA isolation and Polymerase chain reaction Genomic DNA was ... Patient population and genetic analysis We carried out genetic analysis of 13 HIV-1 envelope sequences from Northern India Nine unrelated and mother-child pairs (Pair 1, D & E 57 and Pair 2, D & ... a final extension at 72°C for 10 PCR- amplified DNA was cloned into pGem-T expression vector (Promega Biotech WI, USA) and sequenced in both directions using T7 and SP6-specific primers The sequence...
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báo cáo khoa học: "Isolation of specific and biologically active peptides that bind cells from patients with acute myeloid leukemia (AML)" doc
Ngày tải lên :
10/08/2014, 22:20
... These clones were then profiled with AML and normal cells Sixty-eight (15%) clones bound to CML-BC and AML cells, and were used for further studies with PB and BM cells from normal donors Of these ... HP-B6, 10% with peptides HP-G2 and HPG7, 8% with peptide HP-G5) Peptides HP-C8 and HP-G2 were the least (10–20% of marrow specimens studied) and peptides HP-B4 and HP-G7 were the most likely ... analysis and wrote the manuscript ED participated in study design, isolated the peptides conducted functional studies and analysis AR participated in study concept, design and coordination and wrote...
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báo cáo khoa học: " Targeted isolation, sequence assembly and characterization of two white spruce (Picea glauca) BAC clones for terpenoid synthase and cytochrome P450 genes involved in conifer defence reveal insights into a conifer genome" pdf
Ngày tải lên :
12/08/2014, 03:21
... identification and isolation of TPS and P450 genes using PCR- based screening of a non-arrayed white spruce BAC library of 3X genome coverage, and the subsequent gDNA insert sequencing, sequence assembly, and ... 3CAR (A) and CYP720B4(C) and comparison of 3CAR with the grand fir (Abies grandis) Gene structure of (LIM) and pinene synthase (PIN) genes (B) Gene structure of white spruce 3CAR (A) and CYP720B4 ... constitutive, and wound- and insect-induced expression, and cellular and subcellular localization in spruce and Douglas fir Plant Physiol 2007, 143:410-424 Liu JJ, Ekramoddoullah AK: Identification and...
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Báo cáo y học: " Comparative evaluation of INNO-LiPA HBV assay, direct DNA sequencing and subtractive PCR-RFLP for genotyping of clinical HBV isolates" pot
Ngày tải lên :
12/08/2014, 04:20
... Genotype A and D strains of HBV On the other hand, despite being costly or technically demanding, LiPA and direct DNA sequencing yielded accurate identification of all genotype A and genotype D and ... FH, SA and WAN designed the study and FH collected the specimens MMA carried out the DNA Extraction, INNO-LiPA genotyping assay, Direct DNA Sequencing, PCR- RFLP studies, sequence alignments and ... 265 bp fragment into 41 bp and 224 bp fragments, yielded aberrant results by PCR- RFLP-based method These findings are similar to an earlier report from Uzbekistan The PCR- RFLP-based assay yielded...
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Báo cáo khoa học: " Evaluation of rapid screening and pre-emptive contact isolation for detecting and controlling methicillin-resistant Staphylococcus aureus in critical care: an interventional cohort study" doc
Ngày tải lên :
12/08/2014, 23:21
... JS and DP had the idea for the study and wrote the manuscript SH and CMA developed the study design, drafted the protocol and coordinated its implementation CMA, CA and GR collected the data and ... pre-emptive isolation Rapid PCR technique for admission screening April 2004 to August 2005 (phase III) Systematic on-admission and discharge screening policy General pre-emptive isolation Rapid PCR ... of ICU-acquired MRSA infections Materials and methods Setting and study populations The Geneva University Hospital, Switzerland, is a 2,200-bed primary and tertiary medical centre, to which approximately...
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Affinity guided isolation, structure elucidation and total synthesis of laetirobin a and its analogue synthesis for therapeutic development 1 4
Ngày tải lên :
10/09/2015, 15:48
... sintered glass funnel and concentrated by rotary evaporation The crude extract was partitioned between 1:2 (v/v) acetonitrile and hexanes and the acetonitrile layer was collected and dried via rotary ... 25.0±0.1 µM and 50.0±0.1 µM, as verfied by spectrophotometric analyses Cells were cultured in glass bottom dishes and imaged live after treatment with and washing with fresh media Microtubules and actin ... [(NH4)6Mo7O24•4H2O] and 0.5 g Ce(NH4)2(NO3)6 were dissolved in 400 mL of aqueous 10% H2SO4 Potassium permanganate: 2.5 g KMnO4 and 12.5 g Na2CO3 in 250 mL H2O 176 Appendix Flash chromatography and dry column...
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Affinity guided isolation, structure elucidation and total synthesis of laetirobin a and its analogue synthesis for therapeutic development 8
Ngày tải lên :
10/09/2015, 15:50
... R indices (all data) R1 = 0.1076, wR2 = 0.2243 Largest diff peak and hole 0.606 and -0.620 e.Å-3 X-ray Data 419 Crystal data and structure refinement for 3-26 Identification code 9103 Empirical ... 0.2006 R indices (all data) R1 = 0.1162, wR2 = 0.2258 Largest diff peak and hole 0.919 and -0.317 e.Å-3 420 Appendix Crystal data and structure refinement for 3-3 Identification code 7498 Empirical ... R indices (all data) R1 = 0.0580, wR2 = 0.1169 Largest diff peak and hole 0.215 and -0.172 e.Å-3 X-ray Data 421 Crystal data and structure refinement for 3-61 Identification code a502 Empirical...
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Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx
Ngày tải lên :
07/08/2014, 18:21
... applied for further determination by multiplex PCR and PCR for flagellar H7 antigen detection DNA preparation for Multiplex PCR, flagellar H7 PCR and RAPD analysis E coli O157:H7 strains which ... was collected and used as a template Multiplex PCR for stx1, stx2, eaeA, and hlyA, and the flagellar H7 gene amplification Multiplex PCR for the detection of stx1, stx2, eaeA, and EHEC hlyA gene ... with 70% G+C content resulted in complicated and unrepeatable PCR band patterns [31] Two primers, CRA22 and CRA23, were combined in equimolar ratios and used at 20 pmol per primer per 100 µl reaction...
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Báo cáo khoa hoc:" Development and assignment of bovine-specific PCR systems for the Texas nomenclature marker genes and isolation of homologous BAC probes" ppt
Ngày tải lên :
09/08/2014, 18:21
... BTA27 and BTA29, and could be used as a standard for cytogeneticists using different banding techniques (G, R and Q) Each BAC has just been recently localised by FISH on R-banded and G-banded ... from For ZFY, IGH@ and PGK1, heterologous primers were designed respectively from the buffalo sequence (X99826), and the corresponding human genes (L03677 and M11961 respectively) PCR products were ... interest was conrmed by PCR on the BAC DNA For the three BAC clones identied using PCR systems and BAC for the Texas marker genes 197 Figure Metaphase spread of bovine R-banded chromosomes showing...
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báo cáo khoa học: " Isolation, identification and expression analysis of salt-induced genes in Suaeda maritima, a natural halophyte, using PCR-based suppression subtractive hybridization" doc
Ngày tải lên :
12/08/2014, 03:20
... MIPS functional catalogue and the genes were clustered under different functional categories Expression validation by Northern and real time PCR (qRT- PCR) Slot blot Northern analysis was done ... each set of qRT- PCR reaction for comparison and normalization of the data RNA was isolated as and when required from the leaves of the control and NaCl treated plants as described above and treated ... [33,34], and identification of the convergent and divergent pathways between salinity and other abiotic stress responses and the nodes of signalling convergence may greatly enhance the understanding...
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