isolation molecular biology and chemical characterization of s layers
Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
... dNTPs as a substrate for primer synthesis For this purpose, primase reactions with dNTPs as substrates were performed on M13 mp18 single-stranded DNA at temperatures between and 90 °C Under these ... is both capable of de novo synthesis of DNA primers and of elongating them Long DNA extension products were observed on the ssDNA template when dNTPs were used as substrates, although primase ... those encoding such helicases [12] It has been suggested that both these primases and the associated helicases are the constituent elements of the replication initiation complex of the corresponding...
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Báo cáo hóa học: " Chemical characterization of extra layers at the interfaces in MOCVD InGaP/GaAs junctions by electron beam methods" ppt
... diagnostics of heterostructures with QW layers Semiconductors 2009, 43:1240 14 Bithell EG, Stobbs WM: ’Composition determination in the GaAs/(Al,Ga)As system using contrast in dark-field transmission ... related HAADF intensity values as reference, i.e as internal standards The ratios of the experimental intensity of sublayers and to the intensity of all the inner standards are then compared ... has been switched off This is because the chemical bond strength of Ga-P is greater than that of Ga-As [22], which results in As substitution by P [9,22] Such intermixing is limited to the first...
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Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf
... promastigotes expressing firefly luciferase These cells show constant and stable expression of luciferase, which is directly proportional to the number of live promastigotes The assay was performed ... (University of Mississippi, Medical Center, MS, USA) Parasite cell culture Promastigote cultures of L donovani 1S2 D strain (a gener´ ous gift from L Rivas, Centro de Investigaciones Biologicas CSIC, ... residue, which is replaced by a valine or a serine, respectively This suggests possible involvement of AKs also in CMP/UMP metabolism in leishmania The recombinant leishmanial AK2 was also selectively...
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Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx
... measure reporter gene response Statistical analysis Data was presented as mean ± SEM, and analyzed using one-way analysis of variance and appropriate post hoc test or by Student s t-test using ... luciferase expression was statistically significant (Fig 4) Thus, only the a-isoform is directly coupled to tested signal transduction systems Activation of different cis-elements by the a-isoform ... using PRISM 4.00 software (GraphPad Software, San Diego, CA, USA) Significant differences are denoted with asterisks: *P
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
... regulators of apoptosis [12,13] Caspases are routinely used as a measure of apoptosis, in contrast to necrosis Caspase activation occurs at the intersection of all caspase-dependent pathways and is, ... analysis of VBARP isoforms Exons and introns present in VBARP-L and VBARP -S are represented as boxes with numbers The length in base pairs of each exon is marked on top of the boxes representing ... VBARP-L Results from functional analyses indicate that these transcripts are ubiquitously expressed in human tissues and cell lines at different levels Specific loss of the VBARP transcripts induced...
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Báo cáo khoa học: Sulfation of hydroxychlorobiphenyls Molecular cloning, expression, and functional characterization of zebrafish SULT1 sulfotransferases docx
... and estrogen STs (SULT1E) The hydroxysteroid ST family presently comprises two subfamilies, dehydroepiandrosterone STs (SULT2A) and cholesterol STs (SULT2B) Sequence analysis based on BLAST search ... Detection of zebrafish SULT1 ST1 and ST2 mRNAs in cultured zebrafish cells (lanes and 3) and whole zebrafish (lanes and 4) by RT-PCR The primers used for amplification of zebrafish SULT1 ST1 and were the same ... ST cDNAs (data not shown) Ó FEBS 2003 Molecular cloning of zebrafish sulfotransferases (Eur J Biochem 270) 2407 Fig Amino acid sequence comparison of zebrafish SULT1 ST1 and SULT1 ST2 Residues...
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Báo cáo khoa học: Isolation and enzymatic characterization of lamjapin, the first ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A) ppt
... ribosomal RNAs are rich in the stem-loop structure that is similar to the S/ R domain Perhaps some adenines of these domains also showed certain sensitivity to lamjapin and saporin-R2 Lamjapin is ... one of the few ribosome-inactivating proteins acting at multiple sites in ribosomal RNA Study on the distribution of this class of protein in lower plant is still scarce Most of plant species examined ... previously found only in higher species of plant kingdom Intensive studies by the NMR and X-ray demonstrated that SRD RNA possessed a tertiary structure similar to the S/ R domain of rat liver ribosomes...
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Biotechnology of Microbial Xylanases: Enzymology, Molecular Biology and Application
... Bacillus sp Bacillus sp strain NCL 87-6-10 Bacillus circulans AB 16 Bacillus stearothermophilus SP Clostridium absonum CFR – 702 Rhodothermus marinus a Streptomyces cuspidosporus Streptomyces roseiscleroticus ... thermocellum Bacillus subtilis DB104 Streptomyces sp .S3 8 Streptomyces lividans TK24 and Streptomyces parvulus Streptomyces sp Ec3 Streptomyces lividans Streptomyces thermoviolaceus OPC-520 Escherichia ... allows the passage of lignin or lignin-carbohydrate molecules in higher amounts and of high molecular masses in the subsequent chemical reactions Ligninases and hemicellulases (xylanases) were tested...
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Aircraft measurements over Europe of an air pollution plume from Southeast Asia – aerosol and chemical characterization pot
... air masses themselves must have been influenced to some extent by Asian emissions and, therefore, the excess values are conservative estimates of the impact of the Asian emissions CO is strongly ... typical estimated fire emission strengths Most of the fires were detected on agricultural lands in Myanmar and Thailand The size of these fires is not known and, thus, the emission strength is highly ... were used Most of the results shown in this paper are from the simulations using the ECMWF data but comparisons with results from GFS-driven simulations will also be presented Transport of CO and...
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Báo cáo khoa học: Total chemical synthesis and NMR characterization of the glycopeptide tx5a, a heavily post-translationally modified conotoxin, reveals that the glycan structure is a-D-Gal-(1fi3)-a-D-GalNAc pot
... each species These data demonstrate the feasibility of chemically synthesizing peptides, such as tx5a, that possess multiple post-translational modifications This synthesis in and of itself is a significant ... lg, 34 nmol) ESI and matrix assisted laser desorption mass spectrometry (MALDI-MS) measurement of both components resulted in intense species consistent with the correct product (see below) Both ... configuration is not the same as that previously discovered and reported for contulakin-G (Conus geographus) This surprising result establishes that the O-glycosylation of serine and threonine residues in...
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Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx
... plastids The Ndh complex associates in homodimers and dissociates in membrane and soluble subcomplexes; isolation of the Ndh complex by BN / PAGE and Tricine / PAGE Intact MS and BS chloroplasts ... further analysis of the Ndh subunits by N-terminal sequence analysis or mass spectrometry (MALDI-TOF-MS and MS ⁄ MS) will reveal the complete subunit composition of this incompletely FEBS Journal ... 51 and 23 kDa subunits are assigned as the soluble subcomplex of the Ndh complex The unassigned polypeptides with a molecular mass of 10, 16 and 22 kDa are marked with question marks (?) FEBS...
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Báo cáo khoa học: Molecular and genetic characterization of osmosensing and signal transduction in the nematode Caenorhabditis elegans docx
... and is thus well-suited for studies of osmosensing and associated signal transduction mechanisms Little is known about the molecular bases of these processes in animals The forward and reverse ... what is currently known about the molecular mechanisms of osmotic stress resistance, osmosensing and signal transduction in C elegans Osmosensing and signaling in C elegans Osmoregulatory organs ... that aids in the refolding of misfolded proteins Loss of function of protein homeostasis genes also causes accumulation of damaged proteins and activation of gpdh-1 expression Green arrows and red...
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Báo cáo khóa học: Isolation and biochemical characterization of two soluble iron(III) reductases from Paracoccus denitrificans docx
... ratios, correctly express the substrate specificity of FerB Discussion In this study, we have demonstrated a coexistence of two types of soluble Fe(III) reductases, FerA and FerB, in the soil ... calibration of the column with standard proteins of known molecular masses, the molecular masses of native FerA and FerB were estimated as 25 kDa and Table Purification of P denitrificans Fe(III) reductases ... Fe(III)nitrilotriacetic acid consists of a series of parallel lines Kinetic patterns of this type occur in cases of ping-pong mechanisms At the highest concentration of NADH the initial velocity of the enzyme...
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Báo cáo khoa học: Purification and partial characterization of seven glutathione S -transferase isoforms from the clam Ruditapes decussatus pptx
... conditions as given in Fig Ó FEBS 2002 Purification of R decussatus GST isoforms (Eur J Biochem 269) 4363 Table Biochemical characteristics of R decussatus GST isoforms Results are expressed as means ... apparent molecular mass and the GST-specific enzymatic activities of purified GST isoforms of R decussatus All purified isoforms catalyzed the conjugation of GSH with CDNB, whereas isoforms 1-1, 2-2 and ... alpha and pi class consensus sequences from mammals, respectively The N-terminal sequence of subunit of R decussatus exhibits a high similarity with the corresponding consensus mu GST class from...
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Báo cáo khoa học: "Identification and epidemiological characterization of Streptococcus uberis isolated from bovine mastitis using conventional and molecular methods" pdf
... of two distinct S uberis genotypes, designated as S uberis type I and II According to a proposal of Williams and Collins [68] type II S uberis were classified as S parauberis In the present study ... were classified as S uberis and strain 138/80 as S parauberis The serological investigations revealed that 42 of the 130 S uberis strains, S uberis reference strain NCDO 2038 and S parauberis reference ... Amplicons of S uberis (1, 2, 3) with a size of 1130 bp using the S uberis skc gene specific primers SKC-I and SKC-II; skc gene negative S uberis and S parauberis are shown in lane and M = see Fig...
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báo cáo khoa học: " Isolation and functional characterization of CE1 binding proteins" pptx
... numbers (C) Glucose dose response of RAP2.4L OX lines (D) Glucose dose response of RAP2.4 OX lines (E) Plants grown in the presence of salt for 13 days (F) Salt dose response of RAP2.4L OX lines ... tips including the meristem and the elongation zone Strong GUS activity was also observed in reproductive organs such as sepals, filaments, style and abscission zone The GUS staining pattern of ... mediate stress-responsive abscisic acid signaling Plant Cell 2002, 14:343-357 42 Voinnet O, Rivas S, Mestre P, Baulcombe D: An enhanced transient expression system in plants based on suppression of...
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báo cáo khoa học: " Isolation and functional characterization of cold-regulated promoters, by digitally identifying peach fruit cold-induced genes from a large EST dataset" pptx
... (BSA) as a standard Results Identification of peach cold-regulated genes by digital expression analyses of EST datasets Coordinated gene expression analyses of peach fruit ESTs datasets revealed ... transformation assays, the Pptha1 promoter (pBIPtha1) expressed GUS under all conditions analyzed Discussion and Conclusion Digital expression analyses of EST datasets have permitted us to identify ... mechanisms associated with cold-responsive genes, we may better understand the molecular differences and similarities between cold acclimation and chilling injury as well as the role these processes...
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báo cáo khoa học: " Isolation and functional characterization of Lycopene β-cyclase (CYC-B) promoter from Solanum habrochaites" ppt
... expresses albeit at low level in carpels and sepals, while CrtR-b1, a chloroplast-specific gene shows high level of expression in leaves and sepals but show a low level of expression in flower tissues ... leaves of T0 plants as templates Fruits from transgenic plants of each construct were examined for GUS expression by histochemical analysis The T1 seeds from independent events of each construct ... Figure S1 ) Single copy transgenic plants were carried further for GUS histochemical assay, quantitative MUG assay and Northern hybridization GUS assay The histochemical assay and fluorometric assay...
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báo cáo khoa học: " Isolation and functional characterization of a cDNA coding a hydroxycinnamoyltransferase involved in phenylpropanoid biosynthesis in Cynara cardunculus L" potx
... acyltransferase expression in various C cardunculus accessions and polyphenolic content, especially CGA Our observations suggest this acyltransferase is implicated in the biosynthesis of CGA and its derivatives ... Lineweaver-Burk plots Extraction of polyphenolic compounds, HPLC/DAD and HPLC/MS analyses On the basis of a genetic diversity assessment of 118 globe artichoke accessions [2] about 30 entries were chosen to ... derivatives Heterologous expression of the identified acyltransferase To assess the activity of the globe artichoke isolated acyltransferase, the cDNA was cloned and heterologously expressed in...
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Báo cáo y học: "Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana" ppsx
... analysis of soluble and pellet (inclusion bodies) SDS-PAGE SDS-PAGE analysis of soluble and pellet (inclusion bodies) fractions of the B bassiana proteins expressed in E coli SDSPAGE, Coomasie ... clustering of the known allergens Discussion Figure analysis of the temperature sensitivity of the recombinant SDS-PAGE B bassiana proteins SDS-PAGE analysis of the temperature sensitivity of ... proteins Figure expressed analysis SDS-PAGE analysis of B bassiana recombinant proteins expressed in E coli SDS-PAGE, Coomasie Blue stained, extracts of E coli strain BL21 harboring pRARE and the...
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