isolation depletion using surface markers

... GGC TCC AGT GAC ACT-3' No single surface marker protein has yet been found to characterize MPCs From the accepted markers, we chose seven different cell surface markers and used them in triple ... reactions were performed using a Robocycler® (Stratagene, Amsterdam, The Netherlands) using HotStarTaq™ Master Mix Kit (Qiagen) PCR was performed under linear conditions using the following cycle ... overnight isolation, chondrocytes from OC were directly stained with seven triple combinations of CD9, CD44, CD54, CD90 and CD166 as positive markers, and CD45, CD133/-1 and -2 as negative markers...

Ngày tải lên: 09/08/2014, 01:23

... (circular) horizontal curves Select the to finish the surface creation 10 The resulted surface is shown in the Figure on the left Create another surface at the bottom by selecting Edit pull down ... radius at the bottom edge of the surface Pick the bottom edge of the bottle Click the icon to finish To create a thin solid from a surface quilt, Select the surface quilt first from Model Tree, ... down menu, then choose Fill … Pick the bottom curve Select the creation to finish the surface To merge the two surfaces, select two quilts from the Model tree and click the Merge Tool icon on the...

Ngày tải lên: 25/01/2014, 06:24

... curve created using Equation *What is a surface quilt? A surface quilt is a "patchwork" of connected surfaces A quilt may contain one or more surface patches The external edges of a surface are ... finish the surface creation The resulted surfaces are shown below Merge both surfaces *What is the difference between the Join and No Join options available when creating swept surfaces? Surface ... in magenta *What is a merge? A surface merge allows multiple surfaces to be combined into a single quilt Surface merges are created by selecting Feature, Create, Surface, Merge, and then selecting...

Ngày tải lên: 25/01/2014, 06:24

... select the transformed surface -> Group the cutting surface and cut Feature -> Group -> Cancel the Open window Local Group Type in: cut Select the last two features (transformed surface and cut from ... needed for surface From Sketch pull-down menu, select References 11 Select all the references in the reference window Delete -> Close Pick the , and then click the OK button Copy the cutting surface ... button Copy the cutting surface From Insert pull-down menu, select Surface Operation -> Transform Move -> Copy -> Done Pick the surface just previously created Done Sel Rotate -> CSys Pick the...

Ngày tải lên: 25/01/2014, 06:24

... more intensive three-dimensional bowtie-grooves structure All the simulations were carried out using a finite element solver, 17 HFSS™ The grid size is adaptive with a smallest grid size of nm ... film on a quartz substrate is illuminated by a TMpolarized 400-nm-wavelength plane wave Since the surface grooves are designed to concentrate light in the near-field, the positions of the grooves ... constructive interference This is equivalent to a groove spacing of ␭SPP On the other hand, for focusing light in the near-field, since the SPPs are initially excited at the slit and travel to the...

Ngày tải lên: 06/05/2014, 08:53

... light Surface current is induced on the ridges by the incident photons at the entrance side, flows along the walls of the gap, and reaches the exit side When terminated by the gap, the surface ... Domain Method for Electromagnetics ͑CRC Press, Boca Raton, FL, 1996͒ 18 H Raether, Surface Plasmons on Smooth and Rough Surfaces and on Gratings ͑Springer, Berlin, 1988͒ 19 H Ditlbacher, J R Krenn, ... collimation function completely as the transmitted light spreads around the aperture instead of focusing within the gap due to the excited SP as shown in Fig 2͑b͒ From our calculations, this happens...

Ngày tải lên: 06/05/2014, 08:58

... using surface electromyography Automatic Speech Recognition and Understanding, 2005, IEEE Workshop on 2005, 331-336 Zwarts MJ, Drost G, Stegeman DF: Recent progress in the diagnostic use of surface ... knee torque using a neural network model Journal of Biomechanics 2007, 40(5):1107-1114 18 Hughes RE, Chaffin DB: Evaluation of muscle force prediction models of the lumbar trunk using surface electromyography ... Castellini C, van der Smagt P: Surface EMG in advanced hand prosthetics Biol Cybern 2009, 100:35-47 23 Park W, Kwon S, Kim J: Real-Time Estimation of Thumb-Tip Forces using Surface Electromyogram for...

Ngày tải lên: 19/06/2014, 08:20

... internal [deg] anterior [mm] proximal [mm] medial [mm] geometric SVD 4 -markers SVD 12 -markers PCTu 4 -markers PCTu 12 -markers PCTa 12 -markers -2.3 (2.0) -0.6 (2.2) -1.7 (0.7) 0.5 (3.6) 1.0 (2.4) -2.3 ... using geometrical methods with few exceptions [7] The optimal pose estimators derive the rigid body transformation parameters using least squares methods to solve the difference equation by using ... Twelve markers were attached to the subject's thigh Additional markers were positioned over the lateral and medial femur epicondyles and over the tibia ALs (Figure 2) The position of all markers...

Ngày tải lên: 19/06/2014, 10:20

... Method to improve rice BPH resistance by molecular markers techniques - Evaluating and selecting the F2 to F6 generations on the field test - Using SSR markers to identify BPH resistant gene - Testing ... polymorphic markers between the parental plants (Linkage of RM190 with Bph3 gene) 3.2.3.2 Screening the molecular markers linked with the BphZ(t) gen Figure 3.5 Screening polymorphic markers between ... resistant gene, but has some economic traits 2.2.3 Information collection of molecular markers Most of molecular markers used in this study were collected from the web http://www.gramen.org/cho and...

Ngày tải lên: 13/07/2014, 16:14

... 0.7460 0.6569 0.7225 0.6395 0.7662 information on these markers is presented in Table Five ml of blood was collected from external jugular vein using the vacutainer tube containing EDTA as an anticoagulant ... reactions were carried out using a programmable thermal cycler (MJ Research, USA) Each 20 μl PCR reaction contained 50 ng template DNA (the DNA isolated was diluted using Tris EDTA buffer at in ... information content (PIC) was calculated using the individual frequencies in which the alleles occur at each locus [12] The heterozygosity at the locus was estimated using Nei’s formula [11] The expected...

Ngày tải lên: 07/08/2014, 23:22

... study was carried out to characterize two Indian cattle breeds, Ongole and Deoni using microsatellite markers Using di-and tri- nucleotide repeat loci, 17 Ongole and 13 Deoni unrelated individuals ... initiated to characterize the European cattle breeds using the molecular techniques like microsatellite markers (5,6,7,8,9) Microsatellite markers, by virtue of their codominant and multiallelic ... characterization of these breeds using the reliable molecular markers is scanty In the present study, we undertook the characterization of Ongole (n=17) and Deoni (n=13) cattle breeds using di- and tri- nucleotide...

Ngày tải lên: 09/08/2014, 20:20

... mice, only blood vessels were found to be immunostained using an antibody raised against the C-terminal part of the protein [25,26] Cell surface antibodies directed against metabolite transporters ... external loops Our metabolic markers, all interact with extracellular determinants of the multimembranespanning transporter molecules It must be specified that our markers are independent from ... exclude that a lack of labeling may not be due to the absence of cell surface expression of the transporter but merely to a cell surface environment than hinders ligand binding Thus, it has previously...

Ngày tải lên: 10/08/2014, 05:21

... Systems, Inc., Palo Alto, CA, USA) using daily kilovoltage (KV) imaging, which helped us to clearly visualize the fiducial markers (Figure 5) The position of the fiducial markers was marked on the DRR ... by an orthopedic surgeon (JH) for implantation of fiducial markers for IGRT because of the proximity of the tumor to the spinal cord Markers were placed in the operating room with fluoroscopic ... Solutions, Kalona, IA, USA) was used, with 1.2 mm × mm gold markers pre-loaded in 17gauge sterile placement needles The patient was sedated using general anesthesia A 13-gauge Jamshidi bone marrow...

Ngày tải lên: 10/08/2014, 23:20

... examined in the Cancer Genetic Markers of Susceptibility study using a case-case design A subset of variants (n = 74), showing association within each Cancer Genetic Markers of Susceptibility study, ... slow-growing type of cancer In general, a combination of tumor staging (using the tumor, node, metastasis staging system [22]), tumor grading (using the Gleason scoring system [23]) and diagnostic PSA serum ... Wiklund F: Prostate cancer genomics: can we distinguish between indolent and fatal disease using genetic markers? Genome Medicine 2010, 2:45 ...

Ngày tải lên: 11/08/2014, 12:20

... groundnut The majority of the SSR markers developed to date are derived from the cultivated tetraploid (AB-genome) groundnut using genomic DNA libraries As development of SSR markers from genomic DNA ... different species and sections of Arachis by using the SSR markers developed by Cuc and colleagues [28] from genomic DNA library of cultivated groundnut and SSR markers developed from gene sequences ... http://www.biomedcentral.com/1471-2229/10/15 fragments amplified by using CS SSR markers were of better quality with strong and distinct allelic bands than those obtained by Ah SSR markers which have the problems like stuttering...

Ngày tải lên: 12/08/2014, 03:21

Ngày tải lên: 13/08/2014, 16:21

... the simulations, we determined the number of biallelic markers, heterozygosity of biallelic markers, linkage disequilibrium between adjacent markers and coefficient of determination of QTL Heterozygosity ... variance of that QTL explained by a set of markers R2 was calculated using the equation R2 = c’K-1c, where c is a vector of correlation coefficients between the markers and the QTL, and K is the matrix ... is the matrix of pairwise correlations of the markers When the absolute correlation between a pair of markers exceeded 0.95, only one of these two markers was used to avoid singularity of matrix...

Ngày tải lên: 14/08/2014, 13:21

... strength (!TBS) test and SEM analysis (A) Removal of occlusal surface to expose the flat dentin surface; (B) preparation of the dentin surface to receive adhesive with or without RF; (C) cutting ... 960 cm1 peak [Adapted from Early detection of biomolecular changes in disrupted porcine cartilage using polarized Raman spectroscopy; J Biomed Opt 2011;16(1):017003017003-10 doi:10.1117/1.3528006] ... from SEM evaluation of the interaction pattern between dentin and resin after cavity preparation using ER:YAG laser; Journal of Dentistry (2003) 31, 127–135) ! (! Fig Schematic representation of...

Ngày tải lên: 05/10/2015, 21:24

... Interface 13 2.1.4 Excitation of Surface Plasmons 16 2.1.5 Surface Plasmon Spectroscopy 19 2.2 Fluorescence 23 EXPERIMENTAL METHODS 27 3.1 Surface Plasmon Spectroscopy 27 3.2 Surface Plsmon Fluorescence ... evanescent surface plasmon field near the surface The fluorescence emission mediated by plasmon excitation was characterized DNA hybridizing could be monitored on metallic surfaces using SPFS ... reactions Thus, surface sensitive techniques are essential for the investigation of surface reaction kinetics Unfortunately, many of the surface sensitive techniques such as Surface Plasmon Spectroscopy...

Ngày tải lên: 08/11/2015, 17:01

... volleyball player special physical evaluation, research with the team, the test data of the indicators, using SPSS13.0, Microsoft Excel software for data Statistical Analysis This paper the use of literature, ... volleyball player special physical evaluation, research with the team, the test data of the indicators, using SPSS13.0, Microsoft Excel software for data Statistical Analysis The purpose is to build the...

Ngày tải lên: 13/05/2016, 16:36