isolated enzymes for the transformation and detoxification of organic pollutants

... to the great number of linkages between the enzyme and the support and to the interference of hydrophilic residue of the carrier with the hydrophobic groups of the enzymatic protein The mode of ... for cofactors, substrate delivery, and product removal, may all contribute to the environment and thus to the performance of the enzyme product removal The surface of the carrier, whether inorganic ... into the enzyme active site may be the result of restricted movement of the substrate to the otherwise available active site of the enzyme or of the orientation of the enzyme in relation to the...

Ngày tải lên: 11/08/2014, 15:20

... production [7, 27] These reports, however, lack information on the biochemical and kinetic properties of the secreted enzymes Such information may be useful in gaining better understanding of the lignocellulose ... 600C and 150 rpm for 120 h and the enzyme activities determined thereafter 2.6 Enzyme assays The cellulase and xylanase activity were determined by the assay method of Dutta et al [31] and Cheng ... (filer paper), BWX and PWSD The enzyme kinetic studies for cellulase and xylanase (Km and Vmax) were performed with 1-10 mg/ml of CMC and BWX, respectively [35] The crude cellulase and xylanase were...

Ngày tải lên: 05/09/2013, 16:11

... within the RATEs are catalytically active and that the activity decrease [16] is a result of the tandemization Accessibility of the linker of SGRSGRSG-RATE in the presence of RI The accessibility of ... molecular surfaces, performed with the program sc from the ccp4 suite [27] The obtained sc value of 0.400 indicates weak binding of the two entities of the RATE Therefore, the molecule might become ... relaxed in solution, thereby enabling RI binding to both the N-terminal and the C-terminal RNase A entities The distance between the C-terminus of the first and the N-terminus of the ˚ second RNase...

Ngày tải lên: 22/03/2014, 16:21

... generalisations, the first and third properties leading to the theory of saturated formations and Schunck classes and the associated projectors and the second property to the theory of Fitting classes and ... because of the preceeding argument and applying [Hup67, III, 2.11].This completes the induction step and with it the proof of the lemma Proof (of Theorem 2.2.6) Assume that the result is not true and ... presented The theory of formations was born The new “covering subgroups” had many of the properties of the Sylow and Hall subgroups, but the theory was not arithmetic one, based on the orders of subgroups...

Ngày tải lên: 12/05/2014, 01:59

... an oven and heated at 130°C for h After reaction, the foil was taken out of the autoclave and washed with water for several times and then dried in air X-ray diffraction (XRD) pattern of the products ... in the experiment It gives another evidence for the formation of high purity ZnO product During hydrothermal synthesis of 1-D nanostructures, it was always found that the salt ions existed in the ... solution and the pH values of the solution had great influences on the final products [42, 43] Figure 3a, b is the SEM images of the product obtained when zinc chloride was used instead of zinc...

Ngày tải lên: 22/06/2014, 00:20

... (1) where λ is the wavelength; h is the distance between the middle planes of the graphene and the substrate surface; Ag and As are the amplitudes of the graphene morphology and the substrate ... lithographically patterned for fabrication of devices The atomic force microscopy images indicated the formation of 2-3 layers of h-BNC The structures and compositions of the films were characterized ... C-N Therefore, the issue of stability is the most prominent here The number of strong bonds is sufficiently large in structure V for the stabilization despite of 60◦ arrangement of B-N bonds Another...

Ngày tải lên: 28/06/2014, 10:20

... publish reliable data and information, but the author and the publisher cannot assume responsibility for the validity of all materials or for the consequences of their use Neither this book nor ... kinetics, and mechanism of the enzyme can be brought together in the rational design of an inhibitor However, the transfer of a prospective drug candidate from the laboratory bench to the marketplace ... chemistry at the University of London and received his D.Sc in 1995 A fellow of the Royal Society of Chemistry and the Royal Pharmaceutical Society of Great Britain, he has spent much of his career...

Ngày tải lên: 02/07/2014, 11:20

... axis of one of the profiles using the other profile as the reference One of the replicate spectra from the control condition is chosen as the reference to which the other spectra are warped The ... by incubation for 30 sec This step was followed by the addition of 400 µL of Chloroform and 300 µL of 1M HCL and vortexed for 30 sec The extraction of lipids at acid phase helps in the enhanced ... to the pellet followed by 200 µL of Phenol : Chloroform (1:1) mix The mixture was vortexed in the presence of Glass beads for – Then, 200 µL of TE buffer was added and spun for at 13,000 rpm The...

Ngày tải lên: 04/10/2015, 16:03

... mechanism of Af M1PDH and Af M2DH The theory developed by Cook and Cleland is used to deduce the kinetic mechanism of AfM1PDH and AfM2DH from KIE data in Table [28] The pattern of KIEs observed for ... represents a mean value and standard deviation for three independent experiments evaluated in either of the ways described above The KIE on kcat Km for Fru was calculated from the kcat and Km values obtained ... of Fru concentrations (40800 mm), and so the choice of the constant level of Fru was not critical for determination of DKm (1.23 0.15) The KIE data in Table are instrumental in delineating the...

Ngày tải lên: 14/03/2014, 23:20

... crushing quartz and used for sandpaper The chief types of abrasive sand include sandblast sand, glass-grinding sand, and stone-cutting sand Sand for stone sawing and for marble and glass grinding ... surface; the shadow, light, and 3D visual effect of granite; the look of gun metal; and the glitter of quartz or gold dust A gum resin from the base of the tufted trunk leaves of various species of ... have the radical group ᎏCHO in the molecule, and because of their ease of oxidation they are important reducing agents They are also used in the manufacture of synthetic resins and many other...

Ngày tải lên: 13/02/2014, 09:20

... represents a standard mixture of GlcNAc oligomers showing the standard 60 : 40 ratio between the a-anomer and the b-anomer at equilibrium The other panels show the results of partial hydrolysis of (GlcNAc)6 ... the barley and the jack bean enzymes, ChiG contains an N-terminal extension of eight and seven residues respectively (Fig 1) The N-termini of the plant enzymes and the first residue in the ChiG ... located on the opposite side of the enzyme to the catalytic center and the substrate-binding groove The same applies to the structurally observed N-terminus of the catalytic domain of ChiC, which...

Ngày tải lên: 07/03/2014, 11:20

... parasites of the genus Giardia are one of the earliest lineages of eukaryotic cells, and the Giardia protease is the earliest known branch of the cathepsin B family [23] Its phylogeny confirms that the ... concentrating the allergen according to the isoelectric point (pI) of the molecule A pH gradient from to 11 was established for the characterization of Phl p After completion of the run, the individual ... cysteine residues and other essential amino acids are numbered Areas of high similarity exist around Cys69 and Cys72 and around Trp186, Trp193 and Trp197 The positions of Cys41, 57, 69 and 72 are especially...

Ngày tải lên: 08/03/2014, 22:20

... Suggestions for or descriptions of the end use or application of products or methods of working are for information only and the company and its subsidiaries accept no liability in respect thereof Before ... knowledge of the properties of different phases, it is possible to judge the extent to which they affect the service life of the weldment The diagram can be used for rough estimates of the weldability ... The melting of the wire also reduces the temperature of the molten metal, which also reduces the risk of hot cracking For MIG welding of common steels of type AISI 304, 316, 304L and 316L, wire...

Ngày tải lên: 14/03/2014, 12:21

... crushing quartz and used for sandpaper The chief types of abrasive sand include sandblast sand, glass-grinding sand, and stone-cutting sand Sand for stone sawing and for marble and glass grinding ... surface; the shadow, light, and 3D visual effect of granite; the look of gun metal; and the glitter of quartz or gold dust A gum resin from the base of the tufted trunk leaves of various species of ... have the radical group ᎏCHO in the molecule, and because of their ease of oxidation they are important reducing agents They are also used in the manufacture of synthetic resins and many other...

Ngày tải lên: 14/03/2014, 16:20

... with the value of the bulk SnO2 Quantification of the Sn3d and O1s peaks gives an average Sn/O atomic ratio of 1:1.95, indicating the O-deficient formation of the SnO2 nanowires Fig XRD pattern of ... 1c) clearly indicates that these nanowires are of uniform size and smooth surface, and the average diameter and length of these nanowires are 80 nm and ␮m, respectively The energy dispersive X-ray ... shown in the inset (c) TEM-based EDS spectrum of the single nanowire (d) HRTEM image of the nanowire TEM image (Fig 4a) clearly indicates that nanowires are of smooth surface and rather uniform size...

Ngày tải lên: 19/03/2014, 16:48

... result in the processing line being stopped Another undesirable example is when the force of adhesion of the product to packaging material or to another slice of the product is higher than the breaking ... performed When samples did not fit the normal distribution, the Kruskal–Wallis test was used to test the null hypothesis that the medians of variables within each of the levels of samples were the ... in the range of 1–3 N/cm2 and 4– 12 N mm, respectively About 50% of cooked meat sausages showed BS and EF values within these intervals The galantines had low-values of BS and EF, except for the...

Ngày tải lên: 05/05/2014, 08:43

... than the MCID for the EMS and the original version of the HABAM but not for the PPME These data were not available for the Rasch refined version of the HABAM Validity The PPME has face and content ... in the final stages of drafting of the manuscript and given approval for the version to be published JK contributed to the conception and design of the review, the analysis and interpretation of ... for the HABAM Although the feasibility of the EMS has not been investigated, the HABAM has fewer equipment requirements than the EMS and PPME and is therefore likely to be the more feasible of...

Ngày tải lên: 18/06/2014, 22:20

... and Sa The effects of pull and heat are very similar and not as significant as the effect of velocity Delay and pressure are factors to change the taper length of the pipettes while keeping the ... control measures the velocity of the glass carriage system as the glass softens By increasing the velocity, both the tip size and the surface roughness decrease The velocity has the most significant ... http://www.nanoscalereslett.com/content/6/1/401 Page of 10 Dt Heat Figure The effect of heat on tip diameter and average surface roughness The heat is controlled by the level of electrical current supplied to the filament The unit of heat...

Ngày tải lên: 21/06/2014, 03:20

... function of the thermal conductivities of the base fluid and the nanoparticles, the volume fraction, fractal dimension for particles, the size of nanoparticles, and the temperature, as well as random ... of the nano-copper suspensions, while the effect of the mass fraction of Cu on the viscosity is not as obvious as that of the temperature and SDBS dispersant for the mass fraction chosen in the ... for the large thermal conductivity enhancement of nanofluids [47] However, the Brownian motion contribution to the thermal conductivity of nanofluid was very small and cannot be responsible for...

Ngày tải lên: 21/06/2014, 04:20

... surface of FeOOH nanorods makes the surface smoother and leads to the reduction of the BET surface area In order to confirm the effect of layer-by-layer process on the formation of the uniform FeOOH–Au ... in the final products, and finally dried at 80°C in air For the synthesis of a-Fe2O3–Au nanorods, the as-prepared FeOOH–Au nanorods were heated to 500°C for h in air While for the synthesis of ... From the result of BET analysis, we can find that the BET surface area decrease after the layer-by-lay assembly The possible reason for this phenomenon is that the polyelectrolyte deposited on the...

Ngày tải lên: 21/06/2014, 07:20