is a cruise for youse the whats wheres and whys

... Federal de Viçosa, 36570.000, Viçosa, Minas Gerais, Brazil Authors’ contributions JAQA carried out the experiments, the statistical analysis of the data and drafted the manuscript MTBR and PABR assisted ... and senescenceassociated soybean GmNAC genes (NAC1, NAC5, and NAC6) was determined by quantitative RT-PCR Values for TUN are relative to the DMSO control treatment, and for PEG and AZC the values ... expression to activate an osmotic- and ER-stress integrating pathway, also called the integrated pathway The enhanced accumulation of membrane-associated NRPs activates a cascade to induce the expression...

Ngày tải lên: 11/08/2014, 11:21

Ngày tải lên: 11/09/2016, 20:22

... GCTCAGTGGTGGA-3 and 5-CTGAGGGAAGCAAG AATGGA-3, OXI1 (At3g25250): 5-GACGAGATTATC AGATTTTACGC-3 and 5-AACTGGTGAAGCGGAAG AGAC-3, PTI1-4 (At2g47060): 5-CCCCAAAGAAAATG AGTTGCT-3 and 5-GCATCATTTCCTGGAGGAAAG-3 ... to assess the kinase activity and GST alone was used as a negative control The top panel shows the kinase assay visualized by autoradiography and the bottom panel shows the Coomassie Brillian ... SD agar plates lacking tryptophan, leucine and adenine and the cells were grown for days at 30 °C b-galactosidase agarose overlay assays were performed as described in the Herskowitz laboratory...

Ngày tải lên: 14/02/2014, 19:20

... right.  Unlike Behavioral­Based Procrastination, which is usually caused by a lack of  information or training, Fear­Based Procrastination is caused by, as its name implies,  fear.  Fear is unfortunately a major force in many people’s lives: it’s often a rational, if  ... know, those aren’t the actual cause of your procrastination ­ the cause is fear ­ but they  are the activities we turn to when we are afraid, and they serve to distract us from both  the fear, and the guilty knowledge that we are procrastinating. Procrastination has, in fact,  ... I disagree. As humans, we are subject to death, disease, disappointment, loss,  heartbreak, natural disaster and human­made disaster, among many other afflictions. Fear  is,  in my view, an entirely reasonable response to this reality. ...

Ngày tải lên: 21/02/2014, 22:20

... Klabin and Aracruz that present data for years 2003, 2004 and 2005 and Cenibra which data are available only for 2005 ** The companies not have this value calculated All plants, excepting Aracruz and ... capital formation for the sector International Standart Organization - ISO Forest Stewardship Council - FSC The Brazilian Association for Pulp and Paper -5- Fieldwork: sample and methodology The ... and former President of the Brazilian Society for Ecological Economics She has published widely on trade and sustainable development and has served as a consultant and advisor to the Brazilian...

Ngày tải lên: 22/02/2014, 09:20

... duplex I can be attributed to the distinctive optical features of the 2P analog, rather than to a marked distortion of the DNA conformation 20 3Â-CGGTTGG-AMCGAGA-5Â Conformation of the 2P-containing ... concentrations at saturating AdoHcy concentrations A binding isotherm and corresponding autoradiograph of a typical experiment are shown in Fig The calculated apparent dissociation constants (K dapp ... temperature for 10 and analyzed by 8% native PAGE; (b) incubated with 1% SDS at 65 C for and analyzed by 8% native PAGE; (c) incubated with 0.8% SDS at room temperature for 10 and analyzed by 12% SDS/PAGE...

Ngày tải lên: 07/03/2014, 15:20

... Functional association of Ki-1 ⁄ 57 and PRMT1 D O Passos et al Preparation of cytoplasmic and nuclear extracts, methylation assays with cellular Ki-1 ⁄ 57 and metabolic labeling Carlos H I Ramos and ... (lanes and 2) or were not (lanes and 4) incubated with the inhibitor of endogenous protein methylation Adox, lyzed and fractionated in nuclear (lanes and 4) and cytoplasmic (lanes and 3) fractions ... on SDS ⁄ PAGE and their methylation assessed by autoradiography A parallel gel was analyzed by Coomassie Brilliant Blue staining Lane 6: bacterial 6xHis-Ki-1 ⁄ 57 methylated in vitro was run out...

Ngày tải lên: 16/03/2014, 13:20

... domain (Fig 7B) DISCUSSION There is mounting evidence that inflammatory cell infiltrates play a significant role in driving the pathogenesis of asthma and other allergic diseases by damaging tissue ... 11783–11786 Hanasaki, K., Varki, A. , Stamenkovic, I & Bevilacqua, M.P (1994) Cytokine-induced beta-galactoside alpha-2,6-sialyltransferase in human endothelial cells mediates alpha-2,6-sialylation of adhesion ... tissue and releasing pro-inflammatory agents Activated eosinophils, neutrophils, macrophages and lymphocytes increase in number at sites of inflammation and each are capable of modifying the overall...

Ngày tải lên: 24/03/2014, 04:21

... Ser274 in ik3-1 cDNA with Thr or Ala, mutagenic primers, 50 -TCTCCGGAGATGTCGAACACT CTCAGGTACTCCCAGACCA-30 or 50 -TCTCCGGAGAT GTCGAACACTCTCAGGTGCTCCCAGACCA-30 (corresponding to amino-acid residues ... 0.2 mM phenymethanesulfonyl fluoride and 0.1 mM orthovanadate and sonicated at 8C The cleared supernatants were then incubated for h with the indicated antibodies and precipitated for h with 20 ... Igarashi and Dovie Wylie for expert technical assistance; Drs Jiyong Zhao, S van den Heuvel, Ed Harlow, Andrew Koff, Charles J Sherr, Hiroshi Hirai, Makoto Nakanishi and Hitoshi Matsushime for...

Ngày tải lên: 24/03/2014, 04:21

... 5¢-AATTTC TTCAGTGGCTTTCGCCACATTGAAAAAATCGGT-3¢, 5¢-GATCCTTTCAGCGAGATCCGCAAACATGTCCGC AAACTC-3¢ and 5¢-TTGCAGCATCCAAATGGACGCTT GCAACTTGGCCAATTG-3¢ for H2 5A, H3 7A, D5 2A and K13 4A, respectively The ... (5¢-GCGGGATCCTTAAGCCAAATGGGCTTG-3¢), HPNAP_up (5¢-GCGGAATTCCATATGAAAACATTTG AAATT-3¢) and HPNAP_low (5¢-CCGCTCGAGAGCC AAATGGG-3¢), for pET1 1a and pET29c, respectively The restriction sites for NdeI, BamHI, EcoRI and XhoI are underlined ... and the incubation time ranged from 60 to 120 at °C Lane shows the plasmid DNA, and lane (iron and DNA) and lane (DNA and HP-NAP) correspond to mixtures incubated for 60 Lane (iron and DNA) and...

Ngày tải lên: 30/03/2014, 04:20

... adhesion and activation Three such molecules, LAPP (an approximately 13 kDa leech antiplatelet protein isolated from Haementeria officinalis) and calin and saratin (approximately 65 kDa and 12 kDa proteins, ... significant implications for the use of saratin as a tool to inhibit platelet–collagen interactions, and may provide the basis for the therapeutic use of saratin as a potent antithrombotic agent ... saratin for 10 prior to the flow assay PPACK (40 lm) anticoagulated whole blood was perfused through the chamber for at a wall shear rate of 1000 s)1, and this was followed by washing for at the same...

Ngày tải lên: 30/03/2014, 09:20

... black and white images An area adjacent to the area of interest (A) and an area from an image lacking a specimen (N) were scanned as the background signals When PTPCs were not visible, the focal ... within the 56-amino-acid MADS box at their N-termini and within an adjacent 29-amino-acid region referred to as the MEF2 domain The MADS box is essential for DNA binding and dimerization, and the ... lobes and at the midline in the subesophageal ganglion (SG) (Fig 1A) The axons emanating from the somata of the two pairs of lateral cells extend towards the pars intercerebral with some arborization...

Ngày tải lên: 30/03/2014, 20:20

... inactivation of phosphorylase is associated with both activation and translocation of glycogen synthase, and that the former mechanism alone cannot explain the stimulation of glycogen synthesis ... phosphatase This reverses the inhibition of synthase phosphatase by phosphorylase a There is a long-standing debate as to whether inactivation of phosphorylase is a component of the mechanism by ... synthase activity, with a basal rate of flux at or near the plateau The phosphorylase inactivator, CP-91149, caused both activation of glycogen synthase and translocation of the enzyme to the pellet...

Ngày tải lên: 31/03/2014, 01:20

... engineering and management practices, restoration of coastal areas, habitat restoration in upland areas, water quality efforts, watershed analysis/planning, and habitat creation The councils and NMFS ... hatchery is also aquaculture, but the fish are released before harvest size is reached Artisanal Fishery - Commercial fishing using traditional or small scale manually-operated gear and boats Availability ... protect the stock, and in the case of federal fishery management, are mandated by law to use the best available data The best available data principle sometimes creates a conflict for fishermen In the...

Ngày tải lên: 31/03/2014, 13:20

... certain aspects of x’s character, or character and location, in a way that makes them immediately available for cognitive scrutiny—though, once again, S’s capacity to take advantage of this availability ... clear and comprehensible The technical precision of The Case for Idealism may have a certain virtue in the abstract, but it made enormous, and arguably intolerable, demands on the patience of the ... Greece Guatemala Hungary Italy Japan Poland Portugal Singapore South Korea Switzerland Thailand Turkey Ukraine Vietnam Oxford is a registered trade mark of Oxford University Press in the UK and in...

Ngày tải lên: 10/06/2014, 21:33

... Analysis approach and therefore adopts this specific modality, with the elaborated milestones pathway as the platform for future implementation and evaluation of Training and Fellowship Capacity ... Evaluation culture values evidence as a basis for decision making and supports monitoring of outcomes obtained at different stages Evaluation is viewed as an integral part of planning and managing ... objectives and impacts, identifying and selecting indicators, and participating in data collection and analysis The stakeholders are essential participants in assessing the contributions towards impact...

Ngày tải lên: 18/06/2014, 17:20

... templates for the CAP team to provide guidance and direction for the preliminary analysis of the data Dr Brennan and Ms Marsh then provided feedback on the extracted data provided by the CAP team (in ... for the training manual; and • planning for future training activities for CAP staff at UWA associated with the analysis of the survey data Introduction & Background The underlying aim of this ... data cleaning process was finished during this reporting period and the analysis of the data and report on the descriptive statistics of the survey data and the livestock feed sector in Vietnam...

Ngày tải lên: 21/06/2014, 04:20

... Development of the training manual will be ongoing over the life of the project This activity will last the length of the project and is primarily the task of the Australian team The Australian team met ... data Investigation and documentation of the policy environment facing the sector and SMEs in Vietnam Planning for future training activities and the study tour to Thailand The team has endeavoured ... in analysis of the value chain, industrial organisation, and production economics • Training workshops at IPSARD on survey and data collection techniques; and market analysis, including value...

Ngày tải lên: 21/06/2014, 04:20

... concentrate and sell to remote locations Is there a niche market for SMEs? Need to look at comparative advantage in the whole value chains (e.g for large and smaller mills) and consider social and ... risk with farmers as the agents pay the company directly • Large companies say that small companies can’t compete: they have no capital, can’t buy raw materials to store for months, have no advanced ... increased – there was concern that this required irrigation investment What potential is there to increase corn area and yields? • What risk analysis strategies are in place in the sector – can...

Ngày tải lên: 21/06/2014, 04:20

... stages For broilers, stage lasts on average for about 29 days, stage is the next 48 days and stage is the last 40 days For layers, the respective length of the stages is 94 days, 172 days and ... role for the Vietnamese Animal Feed Association (VAFA) The VAFA could play a strong and useful role for SMEs in the sector The Association is already used by SMEs for feed ration recipes, and advice ... particularly fish meal Small and medium enterprises have less storage capacity, and are able to store for a shorter period than large mills This means they must make material input purchases at...

Ngày tải lên: 21/06/2014, 05:20