iron metabolism in pathogenic trypanosomes
Báo cáo khoa học: Enzymes of mannitol metabolism in the human pathogenic fungusAspergillus fumigatus– kinetic properties of mannitol-1-phosphate 5-dehydrogenase and mannitol 2-dehydrogenase, and their physiological implications pot
Ngày tải lên :
14/03/2014, 23:20
... domains. The N-terminal domain adopts an
expanded Rossmann fold, and provides the residues for
binding the coenzyme. The C-terminal domain has a lar-
gely a-helical structure, and serves mainly in ... of
initial-rate data recorded at different concentrations of
inhibitor. The observed inhibition was, in each case,
best described by competitive binding of coenzyme and
adenine nucleotide. Inhibition ... conditions. Lineweaver–Burk plots of initial-rate data
(Fig. 1) gave a pattern of intersecting lines, for each
enzyme and in both reaction directions, consistent with
a kinetic mechanism in which...
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Tài liệu Báo cáo khoa học: Emerging pathways in genetic Parkinson’s disease: Potential role of ceramide metabolism in Lewy body disease pptx
Ngày tải lên :
18/02/2014, 14:20
... calcium-independent
phospholipase A
2
participates in ER stress-induced
INS-1 insulinoma cell apoptosis by promoting cera-
mide generation via hydrolysis of sphingomyelins by
neutral sphingomyelinase. Biochemistry 46, ... glucosylceramide synthase; SMS, sphingomyelin synthase; SMse, sphingomyelinase; SPT, serine palmitoyl transferase. Yellow repre-
sents enzymes directly involved in ceramide metabolism, in which mutations are ... mutation in the gene encoding
alpha-synuclein in Parkinson’s disease. Nat Genet 18
,
106–108.
42 Singleton AB, Farrer M, Johnson J, Singleton A,
Hague S, Kachergus J, Hulihan M, Peuralinna T,...
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Tài liệu Báo cáo khoa học: Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves doc
Ngày tải lên :
18/02/2014, 18:20
... the main nitrogen compounds in the
phloem exudates, amounting to 51% of the total
amino acids in the light and 56% in the dark
(Fig. 7A). The total amount of alanine, serine and gly-
cine was ... Staining was found in the cyto-
plasmic layers of BSCs (Fig. 4A). No positive staining
was detected in the BSCs using the control sense probe
(Fig. 4B). The localization of GLU mRNAs in the
vicinity ... of
glutamine and asparagine in the phloem seemed to be
increased in the dark (Table 1). Second, glutamate,
aspartate and serine were similarly distributed in leaves
and phloem sap, yielding phloem...
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Tài liệu Báo cáo khoa học: On the mechanism of action of the antifungal agent propionate Propionyl-CoA inhibits glucose metabolism in Aspergillus nidulans doc
Ngày tải lên :
19/02/2014, 16:20
... cells), even in the
mutant strain.
Determination of the intracellular volume
In order to obtain the intracellular concentration of
accumulated acyl-CoA esters, it was necessary to know
the internal ... lmol
NADPặmin
)1
ặmg protein
)1
.
Determination of maintenance
In order to calculate the amount of glucose used for
maintenance, the wild-type strain A26 was used. Four
100-mL aliquots of glucose minimal ... acetate to the media containing glucose
and propionate, the growth rate of both strains increased
and the effect of propionate became less apparent. Finally,
in media containing acetate and propionate...
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Tài liệu Báo cáo khoa học: Elementary modes analysis of photosynthate metabolism in the chloroplast stroma ppt
Ngày tải lên :
20/02/2014, 23:20
... thioredoxin
system [10,11]. Thioredoxin is a small, redox active protein,
capable in turn of reducing or oxidizing disulphide bonds in
proteins. In chloroplasts, thioredoxin is reduced by
ferredoxin, ... authors investigated dark stromal metabolism in
chloroplasts isolated from Mesembryanthemum crystallinum.
This plant is interesting in that it is capable of operating C
3
or
CAM (crassulacean acid metabolism) ... wheat
sedoheptulose-1,7-bisphosphatase in which the regulatory
cysteines were mutated to serines, rendering the resulting
product insensitive to thioredoxin (unpublished data).
Such a change will impact in two ways on...
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IRON METABOLISM Edited by Sarika Arora pptx
Ngày tải lên :
05/03/2014, 23:20
... have provided insight into the mechanisms by which hepcidin modulates
iron absorption. Within a week of being placed on a low -iron diet, rats show a twofold
increase in intestinal iron absorption ... or administration of recombinant hepcidin [51]. The second involves binding of
hepcidin to FPN at the cell membrane, causing internalization and degradation of FPN, thus
diminishing iron transfer ... hepcidin on
intestinal iron absorption. Since the discovery of hepcidin, several authors have reported
that hepcidin expression fails to increase in response to increased iron stores in other...
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Báo cáo khoa học: Enzymes of creatine biosynthesis, arginine and methionine metabolism in normal and malignant cells Soumen Bera1, Theo Wallimann2, Subhankar Ray1 and Manju Ray1 potx
Ngày tải lên :
07/03/2014, 04:20
... creatine kinase in the same malignant cells do not appear to
be sufficient for the building up of an effective creatine ⁄ phosphocreatine
pool. Instead of supporting creatine biosynthesis, l-arginine:glycine ... 91–95.
45 Van Pilsum JF, Martin RP, Kito E & Hess J (1956)
Determination of creatine, creatinine, arginine, guanidi-
noacetic acid, guanidine, and methyl-guanidine in bio-
logical fluids. J Biol ... compounds are in high demand in
proliferating cancer cells.
Abbreviations
3MC, 3-methylcholanthrene; AGAT,
L-arginine:glycine amidinotransferase; CK, creatine kinase; CT-1, creatine transporter;...
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Thyroid hormone metabolism in poultry ppt
Ngày tải lên :
08/03/2014, 09:20
... abundant
in liver, kidney and intestine, medium in several other
tissues and absent in muscle, skin and brain. The D1
activity increases in liver and intestine towards
hatching but decreases in gonads ... is
(mono)deiodination where one iodine is removed from
the outer ring (outer ring deiodination or ORD) or from
the inner ring (inner ring deiodination or IRD) of a
iodothyronine molecule. Deiodination ... levels to reach a
minimum within 30 min after injection, proving the
involvement of pretranslational regulation (Van der
Geyten et al., 1999a). Additional studies including
actinomycine D and cycloheximide...
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Báo cáo Y học: Molecular interaction of neutral trehalase with other enzymes of trehalose metabolism in the fission yeast Schizosaccharomyces pombe pdf
Ngày tải lên :
08/03/2014, 23:20
... Spain
Trehalose metabolism is an essential component of the stress
response in yeast cells. In this work we show that the prod-
ucts of the principal genes involved in trehalose metabolism
in ... be integrated mechanisms.
These findings indicate a divergence in the molecular designs
controlling trehalose metabolism in S. pombe from those in
S. cerevisiae.
MATERIALS AND METHODS
Strains ... TPS1–TPS2
complex in S. cerevisiae, it is tempting to speculate that in
S. pombe these ORFs code for proteins that somehow
regulate trehalose metabolism by interacting with some
members of the enzyme proteins...
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Báo cáo khoa học: Enzymatic features of the glucose metabolism in tumor cells ppt
Ngày tải lên :
14/03/2014, 23:20
... phosphatidylinositol-3-kinase Akt
and mitogen-activated protein kinase are candidate sig-
naling pathways that mediate FASN expression
through the sterol regulatory element binding protein
1c. In breast ... be induced by incubating cancer cells
with oligomycin, an inhibitor of mitochondrial ATP
synthetase [159,160]. Similarly, reduction of OXPHOS
by targeted disruption of frataxin, a protein involved
in ... enzyme becomes inactive
upon phosphorylation either by the cAMP-dependent
protein kinase A or by the insulin-dependent glycogen
A. Herling et al. Tumor specific alterations in metabolism
FEBS...
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Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc
Ngày tải lên :
16/03/2014, 11:20
... the iron box), explaining the regulation of desA
expression by binding of DmdR1 to the iron box. The
same overlapping was found in S. pilosus.
DmdR1 binds to the promoter region of desABCD
in ... evidence for or against this claim. SCO2780
encoding a putative secreted siderophore-binding
lipoprotein with a conserved Fhu (Fe
2+
siderophore
binding) domain showed an iron- limitation response
similar ... desferrioxamine cluster.
Indeed, binding of the pure DmdR1 protein to the
S. coelicolor desA promoter region was shown for the
first time in this work. As described previously, bind-
ing of DmdR1 to the iron...
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Báo cáo khoa học: Pyruvate metabolism in rat liver mitochondria What is optimized at steady state? pptx
Ngày tải lên :
16/03/2014, 14:20
... the input ⁄ output relationship.
Note that by constructing the conversion cone one
loses no information because all other, less interesting,
details are contained in the current matrix.
Assuming ... allowed input ⁄ output behaviour, and
indeed any possible input ⁄ output behaviour is
obtained as a linear combination (with non-negative
scalar coefficients) of these 37 rows of P. Such linear
combinations ... which are
an integral part of a cell in an organ such as the liver.
As already mentioned, such biochemical entities are
tightly integrated in a constantly changing cellular
environment. In other...
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IRON METABOLISM pdf
Ngày tải lên :
22/03/2014, 09:20
... elements are present in living cells. Most of the iron in the
body is located within the porphyrin ring of heme, which is incorporated into proteins
such as hemoglobin, myoglobin, cytochromes, ... review on Cellular Iron metabolism with a
Iron Metabolism
6
Fig. 3. Structure of ferritin showing the outer polypeptide shell with inner iron- core
containing iron stored as mineral –ferric ... showing the physiological turnover of iron in the body
5. Mechanisms regulating Iron absorption
The iron stores in the body are regulated by intestinal absorption. Intestinal absorption of
iron...
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Báo cáo khoa học: A comparative study of methylglyoxal metabolism in trypanosomatids potx
Ngày tải lên :
23/03/2014, 06:20
... following oral dosing. Using this
methodology, we examined the effects of methylglyoxal
on an in vivo T. brucei infection. The maximum achiev-
able methylglyoxal concentration in blood following
oral ... 72 h. Concentrations of inhib-
itor causing a 50% reduction in growth (EC
50
) were deter-
mined using the following two-parameter equation by
nonlinear regression using grafit:
y ẳ
100
1 ỵẵI=EC
50
ị
m
where ... Solid
lines: confirmed metabolism in T. brucei.
Dotted lines: metabolism absent in T. bru-
cei. MeGDH, methylglyoxal dehydrogenase;
LDH, lactate dehydrogenase.
Methylglyoxal metabolism in trypanosomatids...
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Báo cáo khoa học: P NMR studies of energy metabolism in xanthosine5¢-monophosphate overproducing Corynebacterium ammoniagenes pot
Ngày tải lên :
23/03/2014, 17:22
... optimized to gain maximum XMP
production (Table 1). As shown in Table 1, an increase in
the glu/glc ratio (glu/glc) induced a significant increase in
XMP production, and this increase in XMP production ... accumulated
during the glu-consuming phase (phase I), but much
accumulated during the glu-deficient phase (phase II),
suggesting that hypoxanthine production was induced
through a switch in the carbon flux. In ... specifically occurs during XMP production. In
fact, an increase in ATP concentration and ATP/ADP ratio
could be induced during phase I by increasing glu concen-
tration in the culture medium (Fig. 4). Dauner...
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