... mechanisms [42,44] The study ofthe effect of pH on enzyme inactivation allows the calculation ofthe pKa ofthe His176 side chain involved inthe inactivation reaction The rate of inactivation exhibited ... nitrogen atoms ofthe backbone ofthe residue preceding the catalytic Cys The conservation of this interaction is suggestive of its Fig The nal model ofthe clostripain catalytic domain The ribbon ... for details) in smaller face The model is ofthe cleaved clostripain lacking the internal nonapeptide The nal residue ofthe resultant a-chain, Arg181, and the rst residue ofthe b-chain, Ala191,...
... wild-type protein Our data is more consistent with an increased destabilization ofthe protein inthe cytoplasm, a process that, inthe case ofthe Gq familyof proteins, is independent of receptor ... in favor ofthe need of other proteins to stabilize the proteins inthe membrane The interaction of Ga subunits with Gbc could be a stabilizing factor Mutations on Gaq residues which locate in ... observed inthe rate of degradation ofthe remaining mutant protein (20% left ofthe protein) vs the wild 5372 In this report we present evidence that endogenous Gaq, transfected Gaq and Ga16 proteins...
... factors including Ets, GATA, and MyoD/E-box binding factors The introduction of mutation into one ofthe putative Etsbinding sequences suppressed the promoter activity In addition, the specific binding ... assay (EMSA) using the Ets consensus site at )133 As the involvement ofthe Ets consensus binding site at )133 inthe promoter activity ofthe mouse a3 integrin gene was suggested by the luciferase ... triplicate, and the error bars indicate the standard deviation attempted to introduce mutations into these sequences As shown in Fig 8, the introduction of mutation into one ofthe Ets-binding sequences...
... However, the mechanistic roles of several of these residues are not described well and there is no example of mutational analysis of all of these residues inthe same enzyme To obtain an insight into ... 1), thereby confirming the crucial role of Asp142 Catalysis infamily 18 chitinases (Eur J Biochem 271) 259 In most ChiB structures, Asp142 is clearly defined, being either inthe ÔdownÕ or inthe ... positioned inthe ÔupÕ position rows and shows that inthe presence ofthe substrate, rotation of Asp142 towards Glu144 lowers the pKa ofthe latter by 0.8 pH units The calculated effects ofthe D140N...
... their equivalents inthe other subunit ofthe second dimer (Fig 4A) Inthe case of P21212 (TcRpiB-R5P), the four molecules inthe asymmetric unit represent the tetramer The two active sites of ... stages inthe biological cycle ofthe parasite (i.e the epimastigote and the metacyclic trypomastigote inthe insect vector, and the intracellular amastigote and the bloodstream trypomastigote inthe ... Arg141¢ The other end ofthe substrate points into a deep pocket inthe enzyme Moving along the ligand from the phosphate, O4 interacts with His102¢ and a water molecule that is in turn within hydrogen-bonding...
... and Yap1 inthe C-terminal CRD (residues 570–650 in Yap1 and 330–409 in Yap2) was used to further provide an insight into the function Yap2 Domain swapping ofthe Yap1 c-CRD by that of Yap2 has ... twocysteine-based peroxidase functions in a similar way to Orp1 inthe activation of Pap1, the Yap1 orthologue [22] In addition, a second Yap1 redox centre involved inthe direct binding of N-ethylmaleimide ... growth in increasing concentrations of cadmium but not of H2O2 Because ofthe high degree of homology to Yap1, the role ofthe Yap2 cysteine residues may prove relevant for its activation Furthermore,...
... 2000 nM samples, indicating that the low affinity binding ofthe AtPum-HD to the RNA resulted in a dissociation ofthe complex during electrophoresis Thus, the instability ofthe complex did not ... analysis ofthe amino acids located inthe amino-terminal extensions that lie outside ofthe PUM-HD in AtPum proteins A comparison ofthe percentage of amino acid identity and similarity for the amino ... specificity of RNA targets by binding to Page 15 of 19 regions ofthe transcript that lie outside ofthe PUM-HD binding site The recruitment of other factors might also enhance the RNA binding specificity...
... recession, i.e the position ofthe gingival margin with respect to the cement enamel junction (CEJ); • CAL (clinical attachment level): i.e the position ofthe attachment in relationship to the CEJ; ... Considering that no previous report exist reporting on the use of EHA combined with autologous bone but without the application of a membrane inthe treatment of infra bone defects, our clinical ... encouraging considering that in a similar study a mean value ofthe increase ofthe bone height of 0.5 mm was obtain with membrane application [31] CONCLUSIONS All these properties make HA useful in...
... task, then it is not only confined to the Ministry of Labour or Ministry of Education and Training, it is also concerned with human resource planning inthe Ministry of Planning or Ministry of Finance ... and teaching has helped Vietnam inthe new era of integration On the other hand, the learning and teaching of English at the expense of other languages is posing serious issues We should first ... what are their education and training levels etc It is largely concerned in determining what training needs there are so that the labour supply can be shaped to meet the demands ofthe economy...
... the central area for the purpose of resettlement Generally, getting along with the rapid expansion, the east bank ofthe Red River exhibited the beginnings of urbanization The south-west ofthe ... components: Bac Linh Dam (North of Linh Dam), Linh Dam peninsula inthe middle and Linh Dam expansion (South of Linh Dam) The success ofthe project has set the stage for a series of New Towns that ... tropical climate Among these, the outstanding point is the area of highstorey apartments and office buildings inthe center of Linh Dam peninsula, surrounding by low-storey buildings, parks, open...
... nặng nhọc với mức thu nhập thấp, kéo theo điều kiện sống mức tối thiểu, tạm bợ khu nhà trọ rẻ tiền với điều kiện sinh hoạt an ninh không đảm bảo Đời sống tinh thần lao động hạn chế Họ thấy cô ... làm việc chợ lao động thuê trọ theo ngày (đêm), lại thuê nhà theo tháng Có khoảng 22,86% nữ lao động tự phải sống nơi có điều kiện sinh hoạt không đảm bảo, vệ sinh; 40,95% không sử dụng nước ... vật chất, tinh thần lao động nghèo nàn Họ thường tiêu tiết kiệm cho khoản chi phí sinh hoạt thiết yếu Có 22,86% nữ lao động tự phải sống nơi có điều kiện sinh hoạt thiếu thốn, vệ sinh Họ thường...
... that The prevailing direction of wind fall is from the north-west (NW) i.e (315°-00) which is more than 70 % of all the time The frequencies of wind coming from the remaining sectors are insignificant ... average value of wind speed inthe North zone of Iraq during any calendar year A histogram ofthe wind direction measurements for the site of Tuz during this windy month is shown in Figure From ... preparation The northern area of Iraq (the area selected in this study) is considered transition zone because it located between mountain inthe extreme north east and the opening plains inthe south,...
... five definitions, in spite of slight differences inthe expressions, share common features that they all emphasize the importance of finding the closest equivalence in meaning by the choice of appropriate ... inthe translation of exhibit labels inthe Vietnam Museum of Ethnography (VME)? II SCOPE OFTHE STUDY There are several types of exhibit labels in a museum: title or headline labels giving the ... not reflect the role ofthe speaker ofthe House of Commons as an independent person who maintains authority and order in Parliament (b) The SL concept is not lexicalised inthe TL The SL word...
... AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OFTHE USE OF THIS SOFTWARE, EVEN IF ADVISED OFTHE POSSIBILITY ... practically speaking, this means inthe context ofthe user launching the code Good practices include making certain that administrative accounts are kept to a minimum, that administrators use ... developed using script embedded inthe body ofthe email message that executed as the message was rendered for viewing by the client Finally, the recent ILOVEYOU worm was similar in concept to the Melissa...
... costs by reducing the volume of unneeded repairs Non-intrusive Testing and Monitoring for Proactive Maintenance Maintaining the health ofthe network is the prime responsibility of Operations ... causing an increased workload for reconfigurations and maintenance Meeting the challenge of increasing profits inthe face of fewer capital and operating resources to manage this steadily growing and ... is evident in fiber connector cleaning Dirty connectors are the leading cause of poor fiber performance and the most likely failure points inthe network, making periodic cleaning of connectors...
... the command line is actually much more flexible than a windowing interface Unix programs are designed to use together from the command line — as “building blocks” in an almost in nite number of ... combinations, to an in nite number of tasks No windowing system that we’ve seen (yet!) has this tremendous power • You can launch and close windowing programs from the command line, but windowing ... For example, a line inthe section “Exercise: entering a few commands,” near the end of Chapter 1, shows the following: Get today’s date Enter date To follow the exercise, type inthe word date...
... typically, contains a branch instruction to the actual location ofthe interrupt service routine The return from the interrupt service routine, like in a subroutine, pops the top ofthe stack to the program ... by using both the data and the program buses to bring inthe operands ofthe multiplication The data coming from the data bus can be traced in memory by an AR, using indirect addressing The data ... be the label ofthe first instruction ofthe subroutine The assembler and the linker resolve what the actual value is Calling a subroutine has the following effects: • Increments the program counter...
... regime ofthe South Chinese Sea Here, the tidal amplitude is approx 1.5 m The study site Hoa An is located in an area where an interaction ofthe tidal regimes ofthe South Chinese Sea and ofthe ... Hau Giang province in terms of quality and quantity ofthe available water resources Thus, the improvement ofthe rural drinking water supply requires investigations regarding the specific local ... carried out The investigations were carried out by the end ofthe rainy season 2003 The parameters and analytical methods are listed in table The results ofthe analyses were tested on their plausibility...
... occurring in typical kinds of interactions These routines thus can be characterized in broad terms including the kinds of terms typically occurring in given situations, and the order in which the ... because of her insufficient investment in preparing the lessons In summary, we can see from the findings that the interview can really provide in depth the information about the reality of speaking ... of English speaking teaching and learning at Gia Phu High School summary ofthe findings According to the finding from the questionnaire, the realities ofthe students’ inhibition and unwillingness...