interfacial emulsifier protein interactions in emulsions

... respective influence of interprotein and protein solvent interactions Because in practice the protein is displaced from the interface in an aggregated form, the changes in the protein structures ... sodium caseinates Multiple layers seem to be more easily formed in emulsions containing whey proteins than with caseins Because the whey proteins project less into solution than the caseins, they ... C The Interfacial Layer The interfacial layers of many oil -in- water food emulsions contain proteins, which may be mixed with other surfactant materials (Fig 1) Proteins are often present in the...

Ngày tải lên: 23/05/2014, 11:36

... This work Sing et al., 2001 26 Results 3.1 Characterization of NS3-NS3 interaction 3.1.1 Delineating the region of self-interaction in NS3 Protein- protein interactions among all HCV proteins were ... structural proteins (shaded) are located in the N terminus with the nonstructural proteins residing in the remainder of the genome Figure 1-1 HCV Genome and encoded viral proteins RNA Binding nucleocapsid ... implicating its involvement in oncogenesis Taken together, most of the viral proteins interact and may affect the activities of host proteins in the long-term The understanding of the interplay...

Ngày tải lên: 16/09/2015, 17:12

... hydrophobic ones in the later [8] DED-containing proteins are known to participate in diverse cellular functions, including apoptosis through receptor signaling [20,21] Other DED-containing proteins, such ... remains to be determined HIPPI does not have any similarity with known proteins having DNA-binding motifs However, it contains the pDED at the C-terminus (amino acid residues 335–426) and a myosin-like ... N-terminal domain in our system, these two domains cloned in pEGFP C1 vectors were transfected into HeLa cells After 32 h, when 80–90% of cells were expressing GFP-tagged protein, we determined...

Ngày tải lên: 07/03/2014, 10:20

... element containing amino acids 98–110 of the flavivirus E protein) may serve as an internal fusion peptide, as it is directly involved in interactions with target membranes during the initial stages ... residues in the HGV ⁄ GBV-C E2 protein, with sequence homology to the highly conserved loop of the flavivirus E protein described as an internal fusion peptide In this study we have analysed the interactions ... electrostatic interactions play a principal role in the binding of E2(7–26) to negatively charged residues In contrast, a higher contribution of hydrophobic compared with electrostatic interactions...

Ngày tải lên: 07/03/2014, 17:20

... domain fold masked by an extended actin binding tail domain Cell 101, 259–270 29 Balla T (2005) Inositol-lipid binding motifs: signal integrators through protein lipid and protein protein interactions ... of integrins requires talin binding to the cytoplasmic tail of b integrin [13] Talin is an essential integrin-activating protein that connects the cytoplasmic tail of b integrins to the actin ... four-point-one ⁄ ezrin ⁄ radixin ⁄ moesin) domains FERM domains localize proteins to the plasma membrane, where they can interact with phosphoinositides and membrane proteins [21] The FERM domain...

Ngày tải lên: 16/03/2014, 05:20

... in- frame insertions resulting in the insertion of five amino acids into the Cdc37 protein Stop codons were inserted by the five codon insertion in four mutants, each adding only 1–3 extra amino acids ... the human protein may act inefficiently in S pombe blot analysis on GST, S pombe protein extracts and GST-Cdc37 using depleted serum results in a loss of signal against S pombe protein extracts ... domains in human Cdc37 (p50); an N-terminal domain consisting of amino acids 1–126, a middle region composed of residues 128–282 and a C-terminal domain of amino acids 283–378 [8] By aligning...

Ngày tải lên: 16/03/2014, 22:20

... De Kruijff, B (1998) An investigation into the lipid interactions of peptides corresponding to the C-terminal anchoring domains of Escherichia coli penicillinbinding proteins 4, and Biochim Biophys ... been observed for the C-terminal a-helices of Escherichia coli penicillinbinding proteins and [50], which function as membrane anchors for these proteins [51] Interestingly, a recent study has ... penetration indicated for Myr2PtdSer membranes, it is possible that these interactions may involve the snorkelling mechanism that features in the membrane interactions of a number of lipid-interactive...

Ngày tải lên: 17/03/2014, 10:20

... mutating the corresponding residues in E coli FabG involved in the binding of E coli ACP have been investigated earlier, the number of binding sites and ACP-induced cooperativity for the interactions ... positively charged residues to alanines to determine which of the electrostatic interactions are important for promoting the binding to PfACP Interestingly, within the Plasmodium genus, Arg187 is ... conformational changes by monitoring the change in its intrinsic fluorescence In these studies, we have investigated the interactions between PfFabG and PfACP using a combination of computational, biochemical...

Ngày tải lên: 23/03/2014, 10:20

... Kinetics of ostreolysin binding to SEL SUVs (A) Steady-state intrinsic uorescence spectra of 5.13 lgặmL)1 ostreolysin alone (dashed line) or combined with SEL SUVs (solid line) at the lipid /protein ... structural changes in ostreolysin on binding to SEL SUVs, but little or no binding with LUVs containing 10% lyso-PtdIns The collective results (Figs and 8, and Table 2) suggest that binding to the lipid ... and similar proteins, such as Aa-Pri1, are toxins Specic expression of ostreolysin and aegerolysin during initiation of fungal fruiting [4,5], expression of a hemolysin-like protein of B bifermentans...

Ngày tải lên: 23/03/2014, 21:20

... paraformaldehyde in NaCl/Pi for for extracellular staining or with methanol chilled at )20 °C for for intracellular staining After blocking with NaCl/Pi containing 2% BSA (blocking solution) for 30 min, the ... secretion of a soluble integrin-binding protein, MFG-E8, in mouse early gonadogenesis Mech Dev 96, 223–227 23 Salinas, F.A., Wee, K.H & Ceriani, R.L (1987) Significance of breast carcinoma-associated ... specimens were incubated for 60 with the rabbit antiserum raised against the recombinant glutathione S-transferase (GST)–MFG-E8 fusion protein [8] diluted : 150 in blocking solution and then incubated...

Ngày tải lên: 24/03/2014, 03:21

... magainin analog, [Ala8,13,18]-magainin II amide [30] We find that the peptide-induced changes in the transition of DPam2PtdEtn are similar for both magainin II and for [Ala8,13,18]-magainin II ... with liposomes containing PtdGro than with liposomes containing PtdSer [29] We found that b-17 (Fig 6) did not discriminate, in terms of inducing leakage, between vesicles containing Ole2PtdGro or ... Ole2PtdGro or Ole2PtdSer, even though the binding affinity of b-17 to PtdSer-containing vesicles is lower than for PtdGro-containing vesicles Magainin II has been shown in several studies to have antitumor...

Ngày tải lên: 31/03/2014, 07:20

... the binding affinity, the ligand binding site of protein, the amino acids of the protein interacting with ligand, PDB files1 of protein bound to ligand etc) of all the 80 lipid -protein interactions ... the 80 lipid -protein interactions in terms of  their binding affinities,  microscopic atomic interactions between the protein and ligand,  the ligand binding pocket of the proteins,  the active ... in terms of binding affinity between them In other words binding affinity or binding energy is the direct measure of the strength of binding between ligand and receptor Furthermore, the binding...

Ngày tải lên: 28/11/2015, 14:01

... Fyn-SH2 Proteins harbouring SH2 domains bind to phosphorylated tyrosines on their target proteins, thereby coupling the activity of tyrosine kinases with intracellular signalling pathways [28] In ... in proteins [20,21] Tau binding to SH3 domains is regulated by the phosphorylation of tau on specific serine ⁄ threonine residues [21], and we show here that Fyn-SH3–tau interactions are not in uenced ... Results Tau interacts with Fyn-SH2 Because tau contains tyrosines that could be targeted by Fyn, and tyrosine phosphorylation in uences FynSH2 binding to target proteins, we set out to determine whether...

Ngày tải lên: 14/02/2014, 14:20

... electrostatic interactions [60] (d) For the Transient RNA protein interactions in RNA folding human mRNA-binding protein hnRNP A1 [61], the Xenopus laevis protein X1rbpa [54], the trypanosome guideRNA-binding ... the properties of the three protein classes, with the main focus being on RNA chaperones and annealer proteins Specifically binding proteins A specific protein cofactor binds to its RNA target through ... 1637 Transient RNA protein interactions in RNA folding M Doetsch et al Tat peptide induced a change in the population of coexisting and interchanging RNA conformations The lack of intermolecular...

Ngày tải lên: 14/02/2014, 19:20

... investing time and effort in preparing and analyzing the full-length protein can be extremely rewarding, as shown by the information obtained with the tumor suppressor protein BRCA2 and its interaction ... structure facilitating protein binding (indirect) and specific amino acid–base contacts (direct) A recent examination of protein nucleic acid structures has shown how minor groove narrowing enhances ... kon,B koff,B kdt,BC kdt,CB B Fig Proteins find their binding sites in an ocean of DNA sequences Scheme of a nucleic acid-binding protein (gray ellipses) involved in four dynamic equilibria (double...

Ngày tải lên: 14/02/2014, 19:20

... and vaccines intended mainly for military use, the market is simply too small to interest private-sector investment in bringing such products through FDA licensing Furthermore, the vaccine industry, ... manifestation of a continuing dependent relationship that involves three types of interactions: licensing CBW drugs and biologics, especially vaccines; using Investigational New Drugs in military combat ... prescribing both preclinical and clinical research, including the protection of human subjects, in all stages, from animal studies through initial testing in humans to application for Introduction...

Ngày tải lên: 17/02/2014, 11:20

... 53–56 34 Tollin G (1995) Use of flavin photochemistry to probe intraprotein and interprotein electron transfer mechanisms J Bioenerg Biomembr 27, 303–309 Supporting information The following supplementary ... cob(I)alamin from CH3-H4-folate, generating H4-folate and the methylcob(III)alamin form of MS During primary turnover, the homocysteine-binding domain (dotted barrel) and the CH3-H4-folate binding-domain ... dimethylbenzimidazole base is buried within the protein scaffold, well removed from the corrin ring, suggesting that the lower-coordinated Co state preferentially binds to hMS [7] Herein, we report for the first...

Ngày tải lên: 18/02/2014, 08:20

... PDIP46 ⁄ SKAR, including the amino acids of the RNA recognition motif (RRM) also known as the RNA binding domain (Fig 2) [26] Interaction of the human proteins ER and PDIP46/SKAR in vitro In order ... which differ only by an insertion of 29 amino acids in the middle of the protein These proteins are also known either as PDIP46 (polymerase d interacting protein 46) isoform and 2, respectively, or ... potential interactions with other proteins In plants, apart from the identification of ER in the Arabidopsis genome, ER was reported to be induced during programmed cell death in response to infection...

Ngày tải lên: 19/02/2014, 05:20

... observed in the absence of IPTG, likely indicating significant IPTG-independent expression of the protein Using an antibody raised against the recombinant protein, AtuNDH-2 was detected in protein extracts ... domain has been proposed to be involved in the interaction with hydrophobic quinones of respiratory chains [11,13] Respective contributions of both hydrophobic domains to membrane and quinone interactions ... genomic DNA, cloned into pSD80 with a C-terminus (His)6-tag sequence The resulting plasmid was used to transform E coli strain DH10b The recombinant protein, mainly present in membrane fractions...

Ngày tải lên: 19/02/2014, 06:20