interfacial activation and the hydrolytic reaction
Tài liệu Báo cáo khoa học: The swinging movement of the distal histidine residue and the autoxidation reaction for midge larval hemoglobins docx
... essential to check carefully both the initial and nal pH values of the solution As the reaction is started whether from the pH-drop or jump, the protein adjusts itself to the rapid change in pH to reach ... lead to the required rate constant, that is to say kout for the full conversion reaction The situation was the same with the pH-jump experiments Therefore, the practical way to reach the intrinsic ... pocket from the surrounding solvent The iron is thus converted to the ferric met-form, and the water molecule or the hydroxyl ion remains bound to the Fe(III) at the sixth coordinate 6ị In these equations,...
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Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf
... complex The interaction on the one hand between MT1-MMP and GRASP55 and on the other hand between GRASP55 and furin led us to hypothesize that GRASP55 could potentially play a role in the formation ... important role in the formation of the MT1-MMP–furin complex; therefore, 3168 disruption of the interaction between GRASP55 and furin should perturb the activation of pro-MT1-MMP and reduce MT1-MMP ... were found to accumulate in the Golgi apparatus and the TGN in these cells (C Roghi, unpublished data) HT1080 cells were then transiently co-transfected with MT1/EYFP and the GRASP55-green fluorescent...
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Tài liệu Báo cáo khoa học: Activation loop 3 and the 170 loop interact in the active conformation of coagulation factor VIIa pdf
... in the vicinity of the S1 pocket [11,34], in the rst part of activation loop 3, and in the area of the TF-interactive helix and the 170 loop [8,35], areas that are stabilized by TF [9,25,26] The ... conned to the substrate binding cleft (the 170 loop) in the active site region and the S1 pocket, whereas the intramolecular connections, for instance between the TF-binding region and the active ... active site and between the TF-binding region and the activation domain, appear to function normally In order to further elucidate the effects of the G372(223)A mutation, we analysed the mutation...
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Tài liệu Báo cáo khoa học: Activation of the Torpedo nicotinic acetylcholine receptor The contribution of residues aArg55 and cGlu93 ppt
... assembly and ⁄ or play a role in the conformational changes that result in channel activation or receptor desensitization In the case of the Torpedo nAChR, the importance of the a-subunit in ligand ... insignificant shift in the EC50 values for ACh activation to 29 and 47 lm, respectively, and had no significant effect on the cooperativity of receptor activation In contrast, the aR55F and aR55W mutations ... Representation of the extracellular domains of the c–a subunits based on the crystal structure of AChBP The positions of cE93 and aR55 at the subunit interface are indicated and modeling suggests that these...
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Tài liệu Báo cáo khoa học: Proton transfer in the oxidative half-reaction of pentaerythritol tetranitrate reductase Structure of the reduced enzyme-progesterone complex and the roles of residues Tyr186, His181 and His184 pdf
... and are bound between the progesterone and the protein (Fig 7A) In comparison to the oxidized structures, the FMN isoalloxazine ring is less planar, with both the N5 atom and the C8-C7 methyl groups ... Properties of the H181A and H184A enzymes The structure of PETN reductase solved in complex with prednisone, progesterone, 1,4-androstadiene (Fig 1A) and 2-cyclohexen-1-one indicate that these ligands ... proton donation in the oxidative half -reaction of MR [25] PETN reductase is unusual in having two histidine residues (rather than the His-Asn pair seen in OYE, MR and some of the other member proteins;...
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Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
... locks the enzyme in the active form for CTP synthesis and thereby stimulates the kcat of the reaction Since GTP actually will activate the uncoupled glutaminase reaction the structure of the monomer ... )29.7 0.8 kJặmol)1 and )47.3 0.3 kJặmol)1 for the glutaminase reaction and the CTP synthesis reaction, respectively The rst value agrees well with the value of Kishore et al [15] The steady-state ... has to determine the equilibrium constant of the reaction to calculate DH Both the glutaminase reaction and the CTP synthesis reaction of CTP synthase are virtually irreversible and present no...
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A COMPARATIVE STUDY OF THE DIAGNOSIS OF PULMONARY TUBERCULOSIS USING CONVENTIONAL TOOLS AND POLYMERASE CHAIN REACTION* pot
... enormous attention The present study demonstrates the utility and limitations of PCR Among the total of 144 specimens studied, the sensitivity of smear, culture and PCR was 51%, 68% and 91.5% respectively ... like to thank Dr A K Chakraborty and the anonymous reviewers for comments and suggestions on the manuscript, Dr N S Deodhar for comments and suggestion on the work and Drs Anil Ravetkar, former ... diseases other than tuberculosis (Table 1A serial-c) These cases were as follows, two each of asthma, pneumonia and cancer lung, one each of post CABG, COPD and lung abscess and 12 with cough and cold...
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Báo cáo khoa học: Proteolytic activation and function of the cytokine Spatzle in the innate immune response of a lepidopteran insect, Manduca sexta ppt
... produce an active Toll ligand called Spatzle [4,6] The ¨ Spatzle ligand and Toll receptor also establish the dor¨ sal–ventral axis in the Drosophila embryo, although this activation of proSpatzle ... identified in the genomes of the mosquitoes Anopheles gambiae and Aedes aegypti [19,20], but only two Spa¨tzle homologs are present in the genomes of the honeybee Apis mellifera and the red flour ... to B mori Spatzle-1 [23] We performed ¨ 3¢-RACE and 5¢-RACE to obtain the missing ends of the cDNA, and then used primers encompassing the start and stop codons, with larval fat body cDNA as template,...
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Báo cáo khoa học: Small-molecule modulators of zymogen activation in the fibrinolytic and coagulation systems pptx
... govern the two effects The fact that the activation of Glu-plasminogen is a conformationally regulated process is the key to understanding this mechanism The effect of staplabin on the activation ... difference between complestatin and chloropeptin I is only at the position of the C–C bond that connects the indole ring of the tryptophan residue and the aromatic ring of the 3,4-dihydroxyphenylglycine ... the resulting plasmin [25,26] The molecule no longer has the PAN domain and therefore adopts a relaxed conformation [27] because of the lack of intermolecular binding between the PAN domain and...
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Báo cáo khoa học: Role of DptE and DptF in the lipidation reaction of daptomycin ppt
... synthetase DptE To detect the adenylate intermediate, we repeated the reaction with apo-DptF rather than holo-DptF, which should lead to accumulation of the acyl adenylate intermediate The reaction ... phosphopantetheinylation of apo-DptF and apoAcpK Coomassie blue-stained SDS–PAGE gel (left) and in-gel fluorescence (right) of the fluoresceinyl-ACP (+) indicates the reaction with Sfp; ()) indicates the reaction ... digested with BamHI and NotI, and ligated into the same sites of the pQTev vector to yield the plasmid pQTev-dptF The integrity of the plasmid was confirmed by sequencing The resulting plasmid...
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Báo cáo khoa học: Dual mitochondrial localization and different roles of the reversible reaction of mammalian ferrochelatase ppt
... vivo and in vitro [7] Ferrochelatase in the heme-requiring pathogen Haemophilus influenzae functions in the reverse reaction, enabling the bacteria to obtain iron from the host [8] The yeast and ... also exhibit the reverse reaction, although the role of the reverse reaction is unclear [7] Because hemoproteins, including myoglobin and hemoglobin, become substrates of the removal reaction of ... similar to that of the enzyme in the inner membrane, the movement of the enzyme to the outer membrane may occur after the cleavage of the pre-sequence, and may relate to the phosphorylation Mutation...
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Báo cáo khoa học: Calcium-induced activation and truncation of promatrix metalloproteinase-9 linked to the core protein of chondroitin sulfate proteoglycans pot
... had no effect on the position of the two main gelatinase bands (Fig 8, lanes and 2), suggesting that neither the band seen in the stacking gel nor the 300-kDa band at the top of the separating ... indicated under the figure The activation was stopped at the indicated time points by the addition of 10 mM EDTA to the reaction mixture At the right is shown the position of the 225 and 92 kDa forms ... to prevent the appearance of the released forms of MMP-9 from the complex The arrowhead shows the border between the stacking and separating gels, and the arrow shows the position of the 72 kDa...
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Women and Adverse Drug Reactions Reporting in the Canadian Context pot
... in the Health Products and Foods Branch to replace the Therapeutic Products Programme: the Therapeutic Products Directorate (TPD); the Biologics and Genetic Therapies Directorate (BGTD); and the ... – the industry, on the one hand, and the consumer’s health movement, on the other, with government showing favour to both camps, depending on the political climate of the day Women in both the ... consultation with the women’s health community and include the principle of the right to be warned and informed expands regional reporting centres and adequately funds these as the front lines of the reporting...
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Báo cáo khoa học: Mechanism for transcriptional synergy between interferon regulatory factor (IRF)-3 and IRF-7 in activation of the interferon-b gene promoter docx
... depends on the promoter context, which refers both to the specific arrangement of the cis-acting elements in the promoter and to the nature of the factors they bind To investigate the effect of ... important in the context of the IFN-b promoter if the arrangement of the PRDs allows them to occur The importance of these interactions was tested functionally and our results indicate that the balance ... families and the lack of viability resulting from gene targeting of either p300 or CBP places restrictions on the use of mammalian cells to dissect the activation mechanism of the IFN-b gene The IFN...
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Báo cáo khoa học: Upstream and intronic regulatory sequences interact in the activation of the glutamine synthetase promoter pot
... hepatic and muscular origin Materials and methods Sequence of the rst intron of GS The nucleotide sequences of the upstream region and of the rst 1938 nucleotides of the rst intron of the rat ... consist of the sum of a basal activity (produced by the promoter and minimized intron), the effects of the respective upstream (X) and intron (Y) modules, and the interaction (X,Y) between these modules: ... on the promoter Similar to liver cells, the stimulatory effect of either element was largely lost if the other element was absent Furthermore, and again similar to liver cells, the effects of the...
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Báo cáo khoa học: TNFR1-induced activation of the classical NF-jB pathway Harald Wajant1 and Peter Scheurich2 pptx
... IKK complex and activation of the classical NF-jB pathway Instead, we gain more and more evidence that recruitment, modification and activation and inactivation of the E3 ligases and kinases involved ... TNFR1-induced activation of the classical NF-jB pathway, in general, and activation and inactivation of the IKK complex, in particular, is highly dynamic Therefore, the time point considered, as well as the ... TAK1 Whether TAK1 and MEKK3 act in a redundant manner in TNFR1-induced activation of the IKK complex or whether these two molecules cooperate in this regard is currently unclear There is further...
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Báo cáo khoa học: Alanine screening of the intracellular loops of the human bradykinin B2 receptor – effects on receptor maintenance, G protein activation and internalization pdf
... self-maintenance, for the recruitment and activation of the G protein, or for the initiation of the desensitization process (i.e for relaying the information to GRKs and arrestins that the receptor is ... human B2R The sequences of the ICLs, parts of the transmembrane segments (I–VII) and the proximal C-terminus containing the putative helix VIII and the palmitoylated Cys are depicted The clusters ... with the respective construct name next to them The point mutations E66A in the first loop and T242A at the end of the third loop are also indicated The two-dimensional structure of B2R, with the...
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Báo cáo khoa học: Synechocystis DrgA protein functioning as nitroreductase and ferric reductase is capable of catalyzing the Fenton reaction potx
... dialysis and gel filtration, we kept these procedures at a minimum Therefore, it is likely that the difference in the FMN content in the two DrgA protein preparations is due to the difference in the ... [30–34] and, recently, Woodmansee and Imlay [9] proposed that this reaction can be involved in the Fenton reaction both in vivo and in vitro Our DrgA protein also catalyzed the Fenton reaction ... absorption at 340 nm Then, the content of the Tunberg tube was transferred to an anaerobic cuvette using a syringe and the solution was mixed well The reaction was monitored by measuring the consumption...
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Báo cáo khoa học: PKA independent and cell type specific activation of the expression of caudal homeobox gene Cdx-2 by cyclic AM pptx
... approximately 2.5-fold activation (panel ii) Similar results were obtained for two other endocrine cell lines, InR1-G9 and STC-1 (see below, and data not shown) On the other hand, when the same )769 Cdx-2 ... mRNA expression in the FRIC cultures was not apparently affected by forskolin ⁄ IBMX treatment for and h, and the 24-h treatment repressed Cdx-2 mRNA expression On the other hand, proglucagon mRNA ... using the vehicle as the control for the longest time point Figure 3D shows the activation of forskolin ⁄ IBMX treatment on Cdx-2 protein expression in the gut endocrine STC-1 cell line at 1, and...
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Báo cáo khoa học: In vivo cross-linking of nucleosomal histones catalyzed by nuclear transglutaminase in starfish sperm and its induction by egg jelly triggering the acrosome reaction pdf
... as an amine acceptor In the starfish, the jelly coat of the eggs cause sperm to undergo the acrosome reaction [15] The reaction is an essential event in fertilization and requires a Ca2+ influx ... chromatin and suggests that this enzyme is involved in the formation of p28 and p32 in sperm nuclei The acrosome reaction in sperm is necessary for gamete fusion The initiation of the acrosome reaction ... acrosome-unreacted sperm, and found that the acrosome reaction is accompanied by a reduction in the amount of histone monomers and appearance of new bands located between histone H1 and the core histones,...
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