interaction between globular proteins and water soluble polar lipids

... Ka˚re Larsson Interactions Between Proteins and Polar Lipids Tommy Nylander Droplet Flocculation and Coalescence in Dilute Oil-in -Water Emulsions Øystein Sæther, Johan Sjo¨blom, and Stanislav ... food emulsifiers (Krog and Sparsø); by Chapter on proteins and polar lipids (Nylander); by Chapter on food emulsions in general (Dalgleish), and by Chapter 13 on dressings and sauces (Ford et al.) ... surfactants can alter the exchange between b-casein and b-lactoglobulin on an oil water interface Of the two proteins, b-casein is displaced first by both oil -soluble and water- soluble surfactants (18,170)...

Ngày tải lên: 23/05/2014, 11:36

... characterization of NS3 self -interaction, while the second part covers the interaction between NS3 and host proteins, in particular, between NS3 and LMP7 11 Materials and Methods 2.1 Construction ... together, most of the viral proteins interact and may affect the activities of host proteins in the long-term The understanding of the interplay between viral and host 10 proteins will shed light ... structural proteins alone or both structural and nonstructural proteins presented similar phenotypes, including steatosis, oxidative stress, and hepatic tumors The interaction between core and lipid...

Ngày tải lên: 16/09/2015, 17:12

... cloned and expressed in bacteria, and the proteins were purified Interactions of the purified 6X(HN)-pDED and the N-terminal domains of HIPPI [also tagged with 6X(HN)] were studied in vitro by EMSA and ... positive strand, and the sequences AA AGAAAAG () 651 to ) 643), AAAGAAAAG () 725 to ) 717) and GAAGACATT () 849 to ) 857) are present in the negative strand Given that the caspase-8 and caspase-10 ... motifs AAAGAGAAC () 955 to ) 963) in the positive strand, and AAAGAAAAG () 418 to ) 410) and AA AGACATA () 800 to ) 808) in the negative strand, were observed (variants are underlined) As shown...

Ngày tải lên: 07/03/2014, 10:20

... them water soluble and able to associate with model membranes E2(7–26) (GSRPFEPGLTWQSCSCRANG) contains two positively charged Arg residues (Arg9 and Arg23), which could be important for the interaction ... NS3 and E2 proteins of GB virus-C (hepatitis G virus) and its effect on the interaction with model lipid membranes J Peptide Res 61, 318–330 Larios C, Espina M, Alsina MA & Haro I (2004) Interaction ... b-structure and random coil content of the E2 peptides, as calculated using the K2D and the mean residue ellipiticity at 222 nm [33] TFE, trifluoroethanol % a-Helix CONTIN programs, and based on...

Ngày tải lên: 07/03/2014, 17:20

... [23] Rap1 and ICAP-1 regulate Krit1 localization to microtubules and membranes Among the FERM family members, ERM proteins and talin provide a regulated linkage between membrane proteins and the ... buffer and bound proteins were eluted with 200 lgÆmL)1 of FLAG peptide in PD buffer Input and eluted proteins were analyzed by SDS ⁄ PAGE and Sypro Orange staining of the gel Asolectin vesicles and ... of interaction between HypoKrit1DC and Rap1 suggests that Rap1 interacts with the PTB-like F3 subdomain Similarly, talin and radixin FERM domains interact via their F3 subdomain with integrin and...

Ngày tải lên: 16/03/2014, 05:20

... region and is involved in client interactions [8] In the N-terminal 40 amino acids there is 80% identity between the S pombe and S cerevisiae sequences and 50% identity between the S pombe and human ... [15] Identification of biochemical interactions between Hsp90 and Cdc37 in yeast systems is limited In S cerevisiae an interaction between Hsp90 and Cdc37 has been shown using recombinant glutathione ... genetic interaction between Cdc37 and Hsp90 has been observed in S cerevisiae, in that mutations compromised for Cdc37 and Hsp90 function are synthetically lethal [15] Identification of biochemical interactions...

Ngày tải lên: 16/03/2014, 22:20

... USA), and, for each sample, absorbance spectra were produced (Fig 5) These spectra were analysed, and, for those with strong absorption bands, the band parameters (peak position, band width, and ... penicillinbinding proteins 4, and Biochim Biophys Acta 1415, 10–22 Ó FEBS 2002 51 Phoenix, D.A & Harris, F (1998) Amphiphilic a-helices and lipid interactions In Protein Targeting and Translocation ... indicating band peak absorbances For each spectrum, the relative percentages of a-helical secondary structure and b-sheet secondary structure were computed, as described in Materials and Methods, and...

Ngày tải lên: 17/03/2014, 10:20

... that govern the specific interactions between Plasmodium ACP and type II FAS enzymes are poorly understood Here, we report subtle aspects of the interactions between PfFabG and PfACP, with emphasis ... folding of the mutant proteins was verified by analysis of their CD spectrum between 200 and 250 nm (2 nm bandpass) using a JASCO J-810 (Tokyo, Japan) spectropolarimeter at 20 °C and a 0.1 cm path ... (Table 2) and refractoriness to PfACP inhibition (Fig 3) To confirm the importance of Arg187 and Arg230, we used a fluorescence assay to directly monitor the interactions between PfFabG and PfACP...

Ngày tải lên: 23/03/2014, 10:20

... added, and the hemolytic assay was performed as described above The appearance of lipid hydrolytic products of ammodytin I2, i.e lysophospholipids and fatty acids, was conrmed by standard TLC and ... b-sheet; band at 1651 cm)1, a-helix; band at 1640 cm)1, random coil; bands in the region 16381616 cm)1, b-sheet (parallel plus antiparallel) These were used to curve t the original spectrum, and ... with the 90 polarizer, and S are order parameters calculated from the ratio of the parallel and perpendicular absorption bands SL is for the lipid chains, derived from the symmetric and asymmetric...

Ngày tải lên: 23/03/2014, 21:20

... transfected with plasmids containing MFG-E8-L (A and B), MFG-E8-S (C and D), DC1 (E and F) and DC2 (G and H) or empty plasmid (I and J) Transfectants were fixed and stained with the antiserum specific for ... cell surface and secreted MFG-E8 COS-7 cells were transfected with plasmids containing MFG-E8-L (lanes 2, and 12), MFG-E8-S (lanes 3, and 13), DC1 (lanes 4, and 14) and DC2 (lanes 5, 10 and 15) or ... plasmids containing MFG-E8-L (lanes and 2), MFG-E8-S (lanes 3–6) and DC2 (lanes and 8) and were also cultured in a serum-free medium (DMEM) for 24 h COMMA-1D cells (lanes and 10) were cultured in DMEM...

Ngày tải lên: 24/03/2014, 03:21

... set at 467 nm and emission at 539 nm, using nm bandpass slits in excitation and emission and a 490-nm cut off filter in the emission path Polarizers were set at 90° for the excitation and 0° for ... have the structures shown in Fig Materials and methods Materials Phospholipids were purchased from Avanti Polar Lipids (Alabaster, AL) The labeled lipids were made by chemical modification of ... allowed discrimination between the behaviour of the peptide in lipids containing Ole2PtdGro and with eggPtdCho alone Discussion In this study we have characterized the interactions of b-17 with...

Ngày tải lên: 31/03/2014, 07:20

... the ligand,  the intermolecular interactions between the ligand and protein,  the cross reactivity of each ligand with each protein  the potentiality of each ligand to be the drug candidate ... become easy The complex three dimensional structure of proteins and ligands are now available Hence, the atomic interactions between ligand and protein are studied in close proximity There are ... ligands, a set of different targets need to be examined with a set of different ligands Further, the binding affinities between each protein and ligand should be studied and their microscopic interactions...

Ngày tải lên: 28/11/2015, 14:01

... interactions between tau and Fyn-SH3, and this further suggests that different, and possibly independent, mechanisms are involved in these interactions of tau with the same protein Interactions with ... in neuronal DRMs Interactions between tau and Fyn are important for the cellular distribution of these proteins For example, we have shown that the trafficking of tau to DRMs [9] and plasma membranes ... we used GST fusion proteins of Fyn-SH2 and FynSH3 to further investigate the mechanisms by which tau and Fyn interact We determined that tau binds to both Fyn-SH2 and Fyn-SH3, and only the former...

Ngày tải lên: 14/02/2014, 14:20

... the strand displacement event in two ways: by destabilizing edges and bulges, and by favoring the annealing reaction of the invading strand A clear advantage of transient interactions between ... weak, nonspecific interactions Here, the transient interaction of proteins with the RNA molecule destabilizes misfolded intermediates and lowers the free energy of transition states between conformations ... disintegration of the complex Proteins help RNAs to fold and unfold As mentioned above, optimal folding rates of RNA require an energetic balance between local and global interactions within the molecule...

Ngày tải lên: 14/02/2014, 19:20

... of tRNAGln, and therefore cannot adopt their productive forms simultaneously Hinge polypeptide regions between the 1646 catalytic and anticodon-binding domains of the synthase, and between the ... material of large proteins, and the simplification of the system to facilitate crystallization and ⁄ or analysis by other techniques However, investing time and effort in preparing and analyzing the ... of the interaction in solution (Fig 1) The recent assignment of the NMR spectrum of the PCNA ring [6,7] provides the basis for solution studies of its interactions with DNA and other proteins...

Ngày tải lên: 14/02/2014, 19:20

... examine the interactions between DoD and FDA as they affect the development and acquisition of drugs and biologics for CBW defense and (2) to identify potential improvements in those interactions ... complicated three-way interaction between DoD, BioPort, and FDA took place Meetings between BioPort and FDA were attended by as many as 20 to 30 senior DoD officials, both civilian and uniformed, representing ... resolving differences between CBER and ORA; an approach compatible with existing FDA structures and systems; oversight to ensure consistency of decisions and actions; and efficiency and evaluation of...

Ngày tải lên: 17/02/2014, 11:20

... interaction between MSR and hMS, and in particular contact through the FMN domain, analogous to that for the hMS–MSR reactivation complex The sequestering of cobalamin between two partner proteins ... to refine the chaperone-like role of MSR We show that specific protein–protein interactions between hMS and MSR (over and above the need to catalyse the reductive chemistry) are required to promote ... AqCbl and dithiothreitol were replaced with varying concentrations of MSR and 100 lM NADPH The experimental data were fitted to a hyperbolic equation, and yielded a Kact value for MSR of 107 ± nM and...

Ngày tải lên: 18/02/2014, 08:20

... two-hybrid analysis of the interaction between the human proteins ER and PDIP46/ SKAR In order to analyze the details of the interaction between the human ER and PDIP46 ⁄ SKAR proteins a series of ... a homodimer through interactions between the b sheet regions and that phosphorylation at the CK2 sites might disrupt its dimerization and potential interactions with other proteins In plants, ... possibility of the interaction between ER and PDIP46 ⁄ SKAR in the cell Furthermore, both genes, ER and POLDIP3, coding for PDIP46 ⁄ SKAR are expressed in the same set of human tissues and cell lines...

Ngày tải lên: 19/02/2014, 05:20

... of 0.1% TFA in water and 20% of 0.1% TFA in 40% acetonitrile Excitation and emission wavelengths of the fluorescence detector were set at 450 and 525 nm, respectively FAD and FMN standards were ... 2.5 and 30 lg proteins in M and S lanes, respectively Right, immunodetection of purified AtuNDH-2, at 0.1, 0.2 and 0.3 lg protein ⁄ lane from left to right only a small portion being found in soluble ... amperometric and chlorophyll fluorescence measurements performed on E coli membranes and on C reinhardtii thylakoids, AtuNDH-2 is concluded to interact with bacterial membranes and UQs and also with...

Ngày tải lên: 19/02/2014, 06:20