integration of wnt and bmp signals

báo cáo hóa học: " Signaling pathways mediating a selective induction of nitric oxide synthase II by tumor necrosis factor alpha in nerve growth factor-responsive cells" pptx

Ngày tải lên : 19/06/2014, 22:20

... expression of both TrkA and p75NTR [71,72] as well as the convergence of TrkA and p75NTR-mediated signaling impinging upon NF-κB [73] Page 10 of 13 (page number not for citation purposes) Journal of ... design and coordination and provided the expertise for RTPCR and withdrawal experiments HUS sub-cloned the PC12p75NTR(-) cells and participated in study design and result interpretation of experiments ... as mean +/standard error of the mean (S.E.M.), and analyzed by student unpaired two-tailed t test with significance set at p < 0.05 Results Combined NGF and TNFα induce iNOS message and protein...

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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

Ngày tải lên : 14/02/2014, 19:20

... domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions of the v-KIND KIND2 and the MAP2 CD were generated by PCR and cloned ... series of GST-fused deletions of the CD2-1 region (Fig 3E) and examined the interactions between bacterially expressed proteins of these subregions (Fig S1C) and 1654 Fig Of all members of the ... KIND2-binding speciﬁcity of residues 702–744 of MAP2, we performed a pulldown assay of combinations of EGFP-fused KIND1 or KIND2 of v-KIND with GST-fused CD2-1-6-1 or CD2-1-6-2 of MAP2 We successfully...

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Báo cáo khoa học: Liver receptor homolog-1 localization in the nuclear body is regulated by sumoylation and cAMP signaling in rat granulosa cells docx

Ngày tải lên : 23/03/2014, 06:20

... the effect of cAMP signaling on SUMO pathway is complex and cell type dependent Notwithstanding, the up regulation of E1, the down regulation of E2 or E3 and the increased expression of SENP2 lead ... incubating lL of in vitro 35S-labeled product with lg of Aos1 ⁄ Uba2, 150 ng of UBC9 and lg of SUMO-1 in sumoylation buffer (20 mm Hepes, pH 7.5, mm MgCl2 and mm ATP) SAE1 ⁄ SAE2, UBC9 and SUMO-1 ... lL of serum-free medium were placed in a mm cuvette (Bio-Rad, Hercules, CA, USA) A total amount of lg of DNA (2 lg of Myc-tagged LRH-1 expression plasmid, 1.5 lg of pDsRed-SUMO-1 and 1.5 lg of...

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báo cáo hóa học: " Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury" potx

Ngày tải lên : 19/06/2014, 22:20

... Better understanding of the improvement of neurological outcome with gelsolin may allow an in-depth understanding of the mechanisms by which gelsolin attenuates the acute response, and to what ... single dose of gelsolin can dramatically reduce mortality by ameliorating cerebral inflammatory lesions and apoptosis via acceleration of recruitment of monocytes and down-regulation of phospho-ERK1/2 ... manuscript, and participated in the writing of the manuscript QC participated in the design of the study and the preparation of the animal model JRK prepared all the cryostat sections LC and XMZ...

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Báo cáo hóa học: " Resveratrol engages AMPK to attenuate ERK and mTOR signaling in sensory neurons and inhibits incision-induced acute and chronic pain" pptx

Ngày tải lên : 21/06/2014, 19:20

... activation of AMPK (Figure 3A) and a reversible inhibition of both ERK (Figure 3B and C) and mTOR signaling (Figure 3D–H) Hence, resveratrol dose- and time-dependently activates AMPK and inhibits ERK and ... activation of mTOR’s negative regulator, TSC2 [49] This results in a profound inhibition of mTOR and its downstream targets involved in translation control (e.g 4EBP and ribosomal S6 kinase and rS6p ... (Ser473, cat# 4058), total AKT, GAPDH and eIF4A Statistical Analysis and Data Presentation Data are shown as means and the standard error of the mean (± SEM) of eight independent cell culture wells,...

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Báo cáo y học: "Activation of WNT and BMP signaling in adult human articular cartilage following mechanical injury" potx

Ngày tải lên : 09/08/2014, 08:22

... encoding WNT ligands (WNT- 1, WNT5 a, WNT- 5b, WNT- 9a/14, and WNT1 6), receptors (FRZD-1 and FRZD-7), intracellular mediators such as β-catenin, and downstream transcription factors such as TCF and LEF-1 ... upregulation of BMP- 2 mRNA after injury Several factors can determine activation of BMP signaling independently of the expression of one ligand, including secretion and solubility of the ligand(s), ... embryonic skeletogenesis and in the repair of other tissues We detected statistically highly significant upregulation of BMP- 2 mRNA (Figure 2a) and down-regulation of the secreted WNT inhibitor FRZB...

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Báo cáo khoa hoc:" VEGF receptors on PC12 cells mediate transient activation of ERK1/2 and Akt: comparison of nerve growth factor and vascular endothelial growth factor" doc

Ngày tải lên : 11/08/2014, 08:20

... activation of ERK1/2 and Akt after In contrast, NGF produced a stronger and persistent phosphorylation of ERK1/2 and Akt than VEGF165 much more pronounced and prolonged activation of downstream ... MAPK) and Akt activation are sustained for 79.5 h in the presence of NGF (Fig 3, lane 1) Removal of NGF for 7.5 h after 72 hours of differentiation led to a significant reduction of ERK1/2 and ... cell binding of VEGF and endostatins To determine the expression profile of binding partners for murine VEGF164, endostatin and the collagen XV endostatin homologue on neuronal and endothelial...

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Báo cáo y học: "Global analysis of alternative splicing regulation by insulin and wingless signaling in Drosophila cells" ppt

Ngày tải lên : 14/08/2014, 21:20

... Summary of regulated junctions and genes detected upon activation of insulin and wingless pathways (c) Distribution of classes of alternative splicing events for all Drosophila genes (left) and for ... hybridization of the cDNA to the microarray, the ratio of fluorescence between the Cy5 and Cy3 signals was measured, normalized and a Z-score (measuring the statistical confidence of the fold-change ... regions in the same intron of the other 11 Drosophila species Motifs were identified using MEME and PHYLOGIBBS software and the specificity of the enrichment assessed with a set of control sequences...

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Báo cáo khoa học: "Immunohistochemical Localization of Nerve Growth Factor,Glial Fibrillary Acidic Protein and Ciliary Neurotrophic Factor in Mesencephalon, Rhombencephalon, and Spinal Cord of Developing Mongolian Gerbil" ppsx

Ngày tải lên : 07/08/2014, 15:20

... formation of the neuronal and glial pathways Different neurotrophic factors and proteins affected neurons and glia during developmental In the former study regarding the distribution of NGF-, GFAP- and ... M., and Hofmann H.D Expression of ciliary neurotrophic factor receptor mRNA and protein in the early postnatal and adult rat nervous system Neurosci Lett 1994, 180:163-6 Koh S., Oyler G.A., and ... amounts of immunoreactivity such as the substantia nigra and interpeduncular nucleus and to a lesser extent, the central gray matter (Fig 4E) The increase in the number and packing density of the...

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Báo cáo khoa học: "In vitro and in vivo gene therapy with CMV vector-mediated presumed dog b-nerve growth factor in pyridoxine-induced neuropathy dogs" pot

Ngày tải lên : 07/08/2014, 23:22

... function of dog β-NGF (pdβ-NGF) in vitro and in vivo The ultimate goal of this study was to determine the effect of the cytomegalovirus (CMV) vector-mediated gene transfer of the pdβ-NGF in vitro and ... (measure of the ionic balance of the complexes) First, the prepared plasmids were diluted with 200 μl of 5% glucose (w/v) and an appropriate amount of in vivo-jetPET-Gal reagent in 200 μl of 5% ... The phCMV vector and pcDNA2 were digested with Bgl II and EcoR I restriction enzymes, and purified following separation on a 1.5% agarose gel Ligation of the purified phCMV1 and pcDNA2 was performed...

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Báo cáo y học: " Aging, osteoarthritis and transforming growth factor-β signaling in cartilage" pps

Ngày tải lên : 09/08/2014, 07:20

... methods were employed TGF-β isoforms 1, 2, and 3, TGF-β receptors I and II, Smads 2, 3, 4, 6, and 7, and phosphorylated Smad (Smad-2P) were localized in the joints of young and old mice In 2-yearold ... TGF-β-mediated anabolic signaling is an important aspect of joint homeostasis, future studies need to focus on members of the BMP family such as BMP- 2, BMP- 7, and growth differentiation factor (GDF-5) GDF-5 ... Vol No Reddi R-Smads and the C-Smad have a highly conserved Mad homology domains (MH in the amino terminus and MH in the carboxy terminus) References Binding of the TGF-β isoforms to the TGF-β...

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Báo cáo y học: "Nerve growth factor and receptor expression in rheumatoid arthritis and spondyloarthritsi" potx

Ngày tải lên : 09/08/2014, 14:21

... the Mann Whitney U test) Expression levels of trkB and trkC receptors were clearly lower than the ones of trkA and p75 Expression of trkB and trkC in SpA and OA was similar However, trkB expression ... expression levels of the four neurotrophic ligands and the four receptors as determined in the SF cells are depicted in Table and outlined in detail by scatter plots in Figure (ligands) and Figure (receptors) ... assay) Generation of cDNA by reverse transcription and utilization of TaqMan® assay followed the protocol of the manufacturer Table Clinical characteristics of neurotrophins and receptors in the...

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Báo cáo y học: " Increased expression of matrix metalloproteinase-10, nerve growth factor and substance P in the painful degenerate intervertebral disc" pot

Ngày tải lên : 09/08/2014, 14:22

... molecular studies and analysis of results KG participated in the design of the study and cowrote the manuscript JAH conceived of the study, participated in its design and coordination and co-wrote ... according to histological grade of degeneration Values are mean ± standard error of the mean *P < 0.05 Gene expression of interleukin-1 and tumour necrosis factor-alpha and correlation with matrix ... time, this results in the breakdown of matrix until the disc loses both height and function, and in a large proportion of cases, there is innervation and initiation of the pain response which leads...

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Báo cáo y học: "Functional significance of nerve growth factor and its receptor (TrkA) in inflammatory arthritis" pps

Ngày tải lên : 12/08/2014, 14:21

... ng/mL) and interleukin-1-beta (IL-1β) (1 ng/mL) Data are expressed as mean ± standard deviation Nerve growth factor (NGF), TrkA, and p75 data of unstimulated FLSs were compared with NGF, TrkA, and ... upregulated in FLSs of inflammatory arthritis (Table 1), and NGF is mitogenic to FLSs (Figure 1) These observations suggest an autocrine loop of NGF for FLS proliferation and suggest that dysregulated ... proliferation and suggest that dysregulated production of NGF has the potential to influence the inflammatory and proliferative cascades of PsA and RA Abbreviations ELISA, enzyme-linked immunosorbent...

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Báo cáo y học: " Open Access Growth factor signaling in lung morphogenetic centers: automaticity, stereotypy and symmetry" doc

Ngày tải lên : 13/08/2014, 13:20

... and Smads 2, and are expressed in the adjacent epithelium The interactions of subsets of these ligand signals, particularly SHH, BMP4 and FGF10 have been extensively reviewed recently and several ... expression of Bmp5 and Bmp7 has been detected in the mesenchyme and the endoderm of the developing embryonic lung respectively, while Bmp4 expression is restricted to the distal epithelial cells and ... [13,46] Most of the BMP signaling pathway components, such as BMP receptors (type II and type I: ALK2, 3, and 6) and BMP specific receptor-regulated Smads (R-Smads), including Smad1, 5, and 8, are...

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Tài liệu Báo cáo khoa học: Osmotic stress sensing and signaling in ﬁshes doc

Ngày tải lên : 18/02/2014, 16:20

... role of aquaporins as effector proteins of osmosensory signal transduction pathways and the potential role of these proteins in osmosensing of ﬁsh cells has yet to be addressed As a result of recent ... conservation of orthologs of the putative osmosensors TRPV4 and aquaporin and the osmosensory signal transcription proteins TonEBP and TSC22D2 in vertebrates The human sequences of TRPV4 (871 ... mode of integration of signals generated by multiple osmosensors with different sensitivity ranges enables cells to determine the severity of osmotic stress, quantify extracellular osmolality, and...

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Tài liệu Báo cáo khoa học: Osmosensing and signaling in the regulation of mammalian cell function docx

Ngày tải lên : 18/02/2014, 16:20

... cholesterol-enriched microdomains of the plasma membrane (caveolae), intracellular organelles, ligandindependent activation of growth factor- and cytokine receptors and autocrine stimulation of signal transduction ... metabolism and proliferation and provides cytoprotection, whereas cellular shrinkage leads to catabolism and insulin resistance and sensitizes cells to apoptotic stimuli The inﬂuence of cell volume ... activation of the p38-type MAPK and thereby inhibition of 5800 autophagic proteolysis [14] Thus, integrin-dependent cell volume sensing and signaling integrates into the overall context of insulin...

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Tài liệu Báo cáo khoa học: Dual P2Y12 receptor signaling in thrombin-stimulated platelets – involvement of phosphoinositide 3-kinase b but not c isoform in Ca2+ mobilization and procoagulant activity pdf

Ngày tải lên : 18/02/2014, 16:20

... equation of Fluo-3 for Ca2+ [27] Ultra-pure calcium-free water was used for preparation of all buffers and (ant)agonists Measurement of cytosolic cAMP and InsP3 Intracellular levels of cAMP and InsP3 ... thrombin-induced Ca2+ response, and abolished the effects of ADP, AR-C and wortmannin A B C D Fig Contribution of SERCA and phospholipase C to P2Y12-dependent prolongation of Ca2+ responses (A, B) Fura-2-loaded ... importance of the PI3-Kb isoform PI3-Kb and not PI3-Kc mediates P2Y12-dependent procoagulant activity of mouse and human platelets Prolonged elevation in [Ca2+]i can signal for surface exposure of phosphatidylserine,...

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Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx

Ngày tải lên : 19/02/2014, 16:20

... the activation of members of the MAP kinase family The latter relay, amplify and integrate signals from diverse stimuli, thereby controlling the genomic and physiological response of the cells ... CCN2 mRNA levels were normalized to those of GAPDH and the graphical representation of the results of phosphorimage scans of the mRNA hybridization signals is shown as well To compare mRNA expression ... via increased transcription and/ or by stabilization of the CTGF transcripts and the role of RhoA GTPase and p38 signaling in such a regulation, nuclear run-on assays and message stability analyses...

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