inhibition of microbial growth by phenol

... data revealed activation of apoptosis and inhibition of cell growth in embryonic tbx5 morphants at 36 hpf by transcriptional and translational levels By RTPCR, the members of BCL-2 family genes ... ultimately resulted in activation of apoptosis and inhibition of cell growth in whole individual of zebrafish embryo, though knockdown of TBX5 leads to overexpression of both anti-apoptotic and pro-apoptotic ... eventually come to the end sequel of apoptosis and cease of cell growth Besides, the distribution of aberrant apoptosis and deviant cell cycle caused by a deficiency of tbx5 in the embryos might be...

Ngày tải lên: 10/08/2014, 10:20

... SDS/PAGE of both proteins were con®rmed to be as designed except for the ®rst methionine residue of (À4) ECP, which was processed off MTT assay for cell growth The effect of ECP on the growth of various ... 10 lM Effect of ECP on A431 and K562 cells At lM ECP, the growth of K562 cells was completely suppressed whereas growth inhibition of A431 cells was % 50% (Fig 2) As ECP is a member of the supergene ... As the growth inhibition of K562 cells in Fig was shown after days of treatment with ECP, which was Fig Suppression of cell growth in the presence of ECP Left, the percentages of viable cells...

Ngày tải lên: 17/03/2014, 17:20

... translational inhibitory effect of TRAIL This inhibition of translation precedes by several hours the appearance of overtly apoptotic or nonviable cells Binding of TRAIL to its active receptors, ... extent of apoptosis but also the ability of TRAIL to regulate the initiation of translation at the level of eIF2a phosphorylation and 4E-BP1 dephosphorylation Control of protein synthesis by IFNa ... Effects of IFNa treatment on the sensitivity of cells to inhibition of protein synthesis by TRAIL We have previously shown that protein synthesis is rapidly downregulated following exposure of cells...

Ngày tải lên: 19/02/2014, 06:20

... the presence of catalase (50 lgÆmL)1) Catalase slowed the initial loss of hTH activity caused by DA but had no effect on hTH inhibition by NMNorsal or Norsal (n = 4) tetrahydropterin cofactor and ... inhibition Similar to the kinetics of TH inhibition by catecholamines [10,39], enzyme inhibition by NMNorsal is competitive with respect to the tetrahydropterin cofactor and noncompetitive with ... activity in the presence of NMNorsal or Norsal We conclude that hydrogen peroxide formation accelerates TH inhibition by DA, but is not involved in inhibition of the enzyme by 6,7-dihydroxylated...

Ngày tải lên: 07/03/2014, 05:20

... elan-immunoreactive material Fig Evolution of rec-trappin-2 production by Pichia pastoris as a function of the duration of fermentation Aliquots of concentrated supernatants of rec-trappin-2-secreting P pastoris ... Simon, S.R (2001) Kinetics of the Inhibition of Proteinase by Elan Am J Respir Cell Mol Biol 24, 8389 25 Bieth, J.G (1995) Theoretical and practical aspects of proteinase inhibition kinetics Methods ... favor of proteases which widely overwhelm the inhibitory capacity of lung uid The biological signicance of this control mechanism has been highlighted by the observation that the development of...

Ngày tải lên: 07/03/2014, 15:20

... phases of RNAi Ago proteins are key components of RISC responsible for the cleavage of the target RNA The cleavage is catalysed by a Piwi domain of Ago proteins, the structural homolog of RNase ... in the presence of Virus inhibition by DNA interference lead ions, causes the cleavage of virus genomic RNA [15] Here, we extend our studies by analysing the antiviral effects of short and long ... effect of targeted RNA in HeLa cells to be at the level of approximately 78% when 100 nm siDNA was used (Fig 2) Application of higher concentrations of siDNAs than of siRNAs is necessitated by the...

Ngày tải lên: 16/03/2014, 02:20

... investigated Yield (%) of 4-methoxybenzaldehyde 10 11 12 13 14 15 16 17 18 19 Phenol red Phenol red, 24 h Phenol red, pH 3.5 Phenol red, pH 5.5 Phenol red, pH 6.0 Dichlorophenol red Dichlorophenol red, ... voltammetry scans of 0.5-mM phenol red or dichlorophenol red in solutions of different pH were run at a rate of 0.5 VÆs)1 The cyclic voltammetry scans of 0.5 mM phenol red in the presence of 2.5–30 mM ... 10)5 DAÆmin)1) For a direct comparison of the kinetic data of phenol red and 2,4,6-trichlorophenol, the degree of conversion of the two phenols, as a function of time, was plotted (see the equation...

Ngày tải lên: 23/03/2014, 21:20

... hydrolysis of BTC by this enzyme obeyed Michaelis–Menten kinetics In order to investigate the kinetics of this mutant more closely, we measured the hydrolysis of BTC catalyzed by the W82A mutant, by ... concentration of the product was formed The stock solutions of the two enzymes were prepared by dilution of the aliquots with the same amount of buffer The mixture was prepared by mixing together ... Progress curves for the hydrolysis of butyrylthiocholine catalyzed by the horse butyrylcholinesterase, by the W82A mutant of human butyrylcholinesterase and by the enzyme mixture Measurements...

Ngày tải lên: 24/03/2014, 03:21

... expression plasmid of HDV ribozyme Methods The bel7402, HCT116 cells were given by Department of molecular Biology, Shandong University, DNA of HDV ribozyme was synthesized by Shanghai Biosun ... to meet the need of cleavage of telomerase We called the new Figure Structure of antigenomic ribozyme of HDV (g.RZ 1/84) Telomerase RNA plasmid construction We cloned a portion of hTR component ... RT-PCR In normal conditions, only inhibition of the template region can lead to the inhibition of telomerase activity we clone a portion ranging from 19 nt to 88 nt of hTR There are 14 template regions...

Ngày tải lên: 10/08/2014, 10:20

... microfilament aggregation induced by withaferin A is mediated by annexin II Nat Chem Biol 2006, 2:33-38 Jayaprakasam B, Zhang Y, Seeram NP, Nair MG: Growth inhibition of human tumor cell lines by ... Figure inhibition by WFA Quantification of p65 translocation upon PAF stimulation and Quantification of p65 translocation upon PAF stimulation and inhibition by WFA Quantification of p65 translocation ... characterize inhibition of NFκB by WFA, we investigated the effect of WFA on translocation of the NFκB subunit p65 upon stimulation with http://www.journal-inflammation.com/content/6/1/15 Figure inhibition...

Ngày tải lên: 11/08/2014, 08:22

... activity of the MxA gene induced by IFN-a Moreover, our results reveal that the inhibition of MxA gene transcription by WT HBc or its hot-spot mutants has no impact on inhibition of HBV replication by ... collected for detection of HBc protein expression by western blot as described below, and used for extraction of total RNA by Trizol (Invitrogen) Levels of MxA mRNA were assessed by real-time PCR as ... and its three hot-spot mutants not affect the inhibition of HBV replication by IFN-a In order to evaluate whether the inhibition of MxA transcription by HBc protein could decrease the 2.1 IFN A...

Ngày tải lên: 12/08/2014, 01:22

... targets in vitro, inhibition of the specific targets responsible for anti-HIV activity of Prunella in cells remains unclear Identification of constituents of P vulgaris that confer the inhibition to ... Viral titers were determined by infection of ED cells using the single round of infection assay described below Viral infection and time -of- addition studies Inhibition of infectivity studies All ... extracts: 66.8 μg/mL of Ames 27664, 69.2 μg/mL of Ames 27665, 64.2 μg/mL of Ames 27666, or 67.4 μg/mL of Ames 27748) Equivalent quantities of EIAVWSU5 were added to each well of ED cells along with...

Ngày tải lên: 12/08/2014, 04:21

... Luc-Nef + Figure Test of sequence-specificity of the inhibition by HIV-lhNef Test of sequence-specificity of the inhibition by HIV-lhNef (A) Trans -inhibition of HIV-1 production by HIV-lhNef and the ... HIV + pBS + HIV-lhNef Figure Trans -inhibition of HIV-1 by HIV-lhNef Trans -inhibition of HIV-1 by HIV-lhNef (A) HEK239T cells were co-transfected with 150 ng of the HIV-1 molecular clone LAI, HIV-lhNef, ... disadvantage of the use of lhRNA as therapeutic is that the generation of multiple siRNAs will be more likely to cause off-target effects We have previously reported strong inhibition of HIV-1 production...

Ngày tải lên: 13/08/2014, 09:20

... reduction of P-TEFb activity, thus the inhibition of Tat activity is unlikely due to a global reduction of cellular P-TEFb activity To further investigate the mechanism of inhibition of Tatmediated ... HEXIM1-mediated inhibition of Tat required the formation of the P-TEFb/HEXIM1/7SK complex We determined that enhanced expression of HEXIM1 resulted in a modest inhibition (2-fold) of P-TEFb activity ... monitored in the presence of co-transfected Flagtagged deletion mutants of HEXIM1 We found that removal of the C-terminal amino acids affected the inhibition as shown by the HEXIM1 (1–300) and...

Ngày tải lên: 13/08/2014, 09:21

... micrographs of copper surface after 30 days of immersion, x10,000 (a) control experiment at pH of 7.2 (b) 1.0 mg P/L added at pH of 7.2 (c) control experiment at pH of 8.4 (d) 1.0 mg P/L added at pH of ... mechanisms of corrosion inhibition processes A combination of electrochemical studies and surface analysis is well suited for elucidating the mechanism of corrosion inhibition The advent of modern ... conclusion was made by Frignani et al (1999), the introduction of aliphatic substituents into the benzene ring improve the inhibition effects of BTA by promoting the rapid formation of thicker, less...

Ngày tải lên: 22/10/2015, 22:43

... compilation ª 2006 FEBS 371 Inhibition of choline-binding proteins B Maestro et al Fig Effect of choline analogs on S pneumoniae growth (A) State of cultures after h of incubation of S pneumoniae R6 with ... active site of Pce Effect of choline analogs on cell growth and viability Fig Effect of choline and analogs on the activity of cell wall lytic enzymes Data are shown as percentage of activity ... formation of long chains of cells with only small effects on cell growth and viability, even at high concentrations of the compound [24] This effect has been ascribed to the inhibition of the two...

Ngày tải lên: 19/02/2014, 05:20

... expression level of DNA-PKcs protein, so the mechanism of inhibition might involve either hindrance of some essential interaction sites of DNA-PKcs with its functional counterparts or blockage of a conformational ... successfully obtained the soluble DPK3 peptide of ~30 kD with the purity of >95% Identification of the positive phage clones of scFv antibody Following rounds of selection using a human phagedisplay ... http://www.ro-journal.com/content/5/1/70 Page of 11 Figure The effect of anti-DPK3-scFv on apoptosis induction of the xenografted cancer cells in nude mice by irradiation (A) Immunohistochemistry staining of cleaved caspase-3...

Ngày tải lên: 09/08/2014, 09:20

... diffusional resistance of substrate into the biofilm due to the growth of the outer heterotrophic layer over the slower growing nitrifers During Phase 2, the diameter of biofilm particles increased ... Fig Maximum specific growth of heterotrophs Phase 5.00 40 Days of Operation 0 20 40 60 80 Days of Operation Fig Half saturation concentration Ks of heterotrophs Mass fraction of the microorganism ... density of particle decreased from 153 to 97 g/L Heterotrophic bacteria growth had contributed to a less dense biofilm during Phase According to Tijhuis et al (1995), biofilm density of the heterotrophs...

Ngày tải lên: 05/09/2013, 08:40

... ATC, was observed Knockdown of Itch by siRNA suppressed the growth of ATC cells highly expressing Itch, whereas ectopic overexpression of Itch promoted the growth of ATC cells with relatively weak ... this effect, none of them mutually exclusive Activation of Akt by treatment with EGF has been shown to protect cells from TRAIL-induced apoptosis by increasing the phosphorylation of Bad, which impairs ... effect of Itch expression and EGF treatment Importantly, a reduction of Itch expression by siRNA treatment significantly altered the capacity of EGF to protect cells from apoptosis Cell survival of...

Ngày tải lên: 16/02/2014, 09:20

... Korsmeyer SJ & Streuli CH (2002) Activation of BAD by therapeutic inhibition of epidermal growth factor receptor and transactivation by insulin-like growth factor receptor J Biol Chem 277, 27643–27650 ... types of stimuli activate JNK through phosphorylation by the dual-specificity kinase MKK4 or MKK7 [18,25] By contrast, any types of stimuli can inactivate JNK through induction of the expression of ... was not affected by AG1478 treatment, indicating that the expression of MKP-1, but not that of MKP-2, is controlled by signals via EGFRs Brondello et al reported that activation of the ERK cascade...

Ngày tải lên: 18/02/2014, 13:20