informatics for efficient est based gene discovery in normalized and subtracted cdna libraries
Báo cáo y học: "Regulation of catabolic gene expression in normal and degenerate human intervertebral disc cells: implications for the pathogenesis of intervertebral disc degeneration" ppt
Ngày tải lên :
09/08/2014, 14:20
... important in inflammatory responses involving IL-1β and MMP-3 and -9 in murine brain injury [26] This suggests that, although TNF-R1 levels remain unchanged and TNF-R2 gene expression is increased, ... expression of interleukin-1 and interleukin-1 receptors The addition of recombinant TNF-α for 48 hours resulted in a significant upregulation of IL-1β and IL-1Ra genes in both normal and degenerate ... and IL-1α and further upregulates TNF-α This would result in an increase in matrix-degrading activity and accelerating degeneration and would be consistent with the suggestion that TNF-α is involved...
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Báo cáo y học: " Novel venom gene discover Novel venom gene discovery in the platypusy in the platypus" pot
Ngày tải lên :
09/08/2014, 22:23
... domains - Unknown Mamba intestinal toxin-like 56 MIT1 (snake) Open cation channels; unknown C-type lectin domain-containing 38 Rhodocytin (snake); however, contains several additional domains ... venom PIII metalloproteinases, containing preprosequence, metalloproteinase, disintegrin, and cysteine-rich domains [28] (Figure 4) This conservation of domain and domain order across such widely ... and reptiles again suggests the selection of certain peptide motifs for evolution to venom molecules Metalloproteinases Seven genes encoding PIII zinc metalloproteinases, which contain the zinc...
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báo cáo khoa học: " Meiosis-specific gene discovery in plants: RNA-Seq applied to isolated Arabidopsis male meiocytes" docx
Ngày tải lên :
11/08/2014, 11:21
... Conclusion: These findings advance our understanding of meiotic genes, gene expression and regulation, especially the transcript profiles of MGI genes and TE genes, and provide a framework for functional ... expressed TEs in meiocytes and anthers NADH-ubiquinone proteins (5%), which suggest this group of genes may function in meiosis (Figure 5C) A preliminary investigation on the T-DNA insertion lines targeted ... potential functions in meiosis These findings provide a framework for future functional analysis of genes in meiosis and advance our understanding of meiotic genes, gene expression and regulation...
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báo cáo khoa học: " An EST-based analysis identifies new genes and reveals distinctive gene expression features of Coffea arabica and Coffea canephora" potx
Ngày tải lên :
11/08/2014, 11:21
... cold and drought, and during fruit ripening [84,85] ABA/WDS are proteins Cterminally enriched in His and Lys and are induced during ripening in pummel [86] and under water deficit stress in loblolly ... ADP-ribosylation factors and proteins containing RRM and G-patch motifs, involved in RNA binding activity [45] Orthologous Family Clustering: Searching for CoffeeSpecific Families To identify proteins that are ... can provide targets for transgenesis, an interesting tool for genetic improvement of such a long generation time crop as coffee In fact, data in coffee genetic transformation indicate the potential...
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Báo cáo y học: "A genome wide analysis of the response to uncapped telomeres in budding yeast reveals a novel role for the NAD+ biosynthetic gene BNA2 in chromosome end protection" doc
Ngày tải lên :
14/08/2014, 21:20
... cdc13-1 cdc13-1B-Ndatagenesgenesexpressed1-13, strains genes are and Transcription expressedofalteredinfactor genesandmicroarray in in AdditionalforGOstats inanalysisgenecdc13-1genes TableTable Bcell ... transcription factor genes up-regulated and down-regulated in cdc13-1 strains strains in of fileofgenesQT showgenesduring both tlc1Δ C Din time Tablehere cdc13-1ofgenesshockin CTT1 cdc13-1cdc13- 1in cdc13-1 ... DDR kinaseencoding gene DUN1, which is up-regulated in cdc13-1 strains and in response to other cellular insults [27,51] Interestingly, DUN1 is also induced in senescent human retinal pigment Volume...
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The Potential of Biofumigants as Alternatives to Methyl Bromide for the Control of Pest Infestation in Grain and Dry Food Products
Ngày tải lên :
25/10/2013, 05:20
... protection has increased and stands as the main alternative to methyl bromide Lately, insect resistance to phosphine has become an important issue for effective grain treatment (Nakakita and Winks, 1981; ... phosphine is a quick and effective tool for the control of stored-product insect pests In view of the scheduled phaseout of methyl bromide under the Montreal protocol, the role of phosphine in grain ... (Schuhmacher et al., 2003), and repellents against ectoparasites (Mumcuoglu et al., 1996) The efficacy of essential oils as fumigants for the control of pest infestations in grain and dry food products...
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Ambient particulate air pollution induces oxidative stress and alterations of mitochondria and gene expression in brown and white adipose tissues ppt
Ngày tải lên :
06/03/2014, 19:20
... long chain fatty acids (Elovl3), type iodothyronine deiondinase (Dio2), homeobox C9 (Hoxc9), insulin-like growth factor binding protein (Igfbp3), dermatopontin (Dpt), and b-actin are showed in Table ... standard deviation inflammatory response and IR development, were examined in mice As shown in Figure 3, PM 2.5 exposure induced a marked increase in macrophage (F4/80+ cells) infiltration in ... reduced in both eWAT and iBAT in response to PM2.5 exposure Recent studies have implicated PM2.5 in increased adipose inflammation and insulin resistance [11,12], and epidemiological studies indicate...
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New ESPGHAN guidelines for the diagnosis of Coeliac Disease in Children and Adolescents pptx
Ngày tải lên :
22/03/2014, 09:20
... serological testing *Or specific IgG based tests Asymptomatic Person at Genetic Risk for CD Explain implication of positive test result(s) and get consent for testing HLA DQ testing (+/‐Anti‐TG2) ... further serological testing *Or specific IgG based tests Asymptomatic person at genetic risk for CD Explain implication of positive test result(s) and get consent for testning HLA DQ2 / DQ8 (+/- ... evidence -based guidelines for CD diagnosis AHRQ (USA, 2004) Adults and children NICE guidelines (UK, 2009) Adults and children For GP’s and general paediatricians None questioned...
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The welfare state as a determinant of women’s health: support for women’s quality of life in Canada and four comparison nations pptx
Ngày tải lên :
22/03/2014, 11:20
... weakening of social infrastructure along the lines seen in the UK and US and to a lesser extent in Canada 9.1 Decline of the welfare state Teeple [11] sees increasing income and wealth inequalities ... Act and therefore there is wide disparity among provinces in availability of services Provincial governments are responsible for setting policy, planning, monitoring and regulations Financing ... Income share—poorest 10% Income share—poorest 20% Income share—richest 10% Income share—richest 20% Richest 10% to poorest 10% Richest 20% to poorest 20% Gini index Denmark 27627 3.6% 9.6% 20.5% 34.5%...
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Báo cáo khoa học: Differential gene expression in periportal and perivenous mouse hepatocytes potx
Ngày tải lên :
23/03/2014, 10:20
... same holds true for the gene encooding ATP citrate lyase (EC 2.3.3.8), an enzyme forming oxaloacetate and acetyl-CoA from citrate for further utilization in gluconeogenesis and cholesterol synthesis, ... demonstrates differences in expression of the genes encoding enzymes of histidine and serine ⁄ glycine catabolism Three genes encoding enzymes of histidine catabolism, histidine ammonia lyase (EC ... localized in the periportal area, namely ornithine transcarbamylase (EC 2.1.3.3), argininosuccinate synthetase (EC 6.3.4.5), argininosuccinate lyase (EC 4.3.2.1), and arginase (EC 3.5.3.1), showing...
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Báo cáo Y học: Selection of effective antisense oligodeoxynucleotides with a green fluorescent protein-based assay Discovery of selective and potent inhibitors of glutathione S-transferase Mu expression doc
Ngày tải lên :
31/03/2014, 15:20
... EGFPcontaining proteins were visualized with enhanced chemiluminescence The positions and molecular weights of the marker proteins are indicated in the left margin In the right margin, the estimated ... performed after culture in the serum-containing medium for a further 18 h analysed for GFP expression by Western blotting Five lg total cellular protein, dissolved in denaturing loading buffer (62 mM ... overhanging ends (underlined) to enable ligation in EcoRI and BamHI restricted pEGFP-C1 Combining the last two PCR reactions results in the formation of GSTM2 cDNA with EcoRI/BamHI overhanging ends...
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semantic web for the working ontologist effective modeling in rdfs and owl
Ngày tải lên :
31/05/2014, 01:56
... experience and knowledge in evaluating and using any information, methods, compounds, or experiments described herein In using such information or methods they should be mindful of their own safety and ... simple queries In particular, SPARQL can be used to specify general inferencing in a concise and precise way We have adopted it as the main expository language for describing inferencing in this book ... variety of content, making them less and less useful as discriminators for people browsing for content This sort of problem is inherent in information modeling systems; since there isn’t an objective...
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báo cáo sinh học:" A technical framework for costing health workforce retention schemes in remote and rural areas" ppt
Ngày tải lên :
18/06/2014, 17:20
... of financing and of assessing financial sustainability Finally, this paper discusses and investigates the role and importance of costing in a broader discussion on how to improve health workforce ... contain any relevant information related to resource use, financing or costing [11-19] These matched the inclusion criteria listed above, but even within these, the information on costing and ... policy intervention Source and mode of financing Identification of key inputs/resources Focusing on key incremental inputs/resources Monetary evaluation of incremental inputs/resources Accounting for...
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Báo cáo hóa học: "Research Article A Novel Criterion for Writer Enrolment Based on a Time-Normalized Signature Sample Entropy Measure" pptx
Ngày tải lên :
21/06/2014, 19:20
... training set for both statistical classifiers For test purposes, the remaining 25 genuine signatures and 20 skilled forgeries (belonging to two sessions) are used for DS2-104 For MCYT-100, we tested ... assessment, both skilled and random forgeries are considered Ten random samplings are carried out on genuine and impostor signatures in the following way: each sampling contains five genuine signatures ... categories, for both skilled and random forgeries: GMM and HMM classifiers give the best performance on writers belonging to the category of lowest Personal Entropy, that is, those having the longest...
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Báo cáo y học: "Histone deacetylase inhibitors modulate metalloproteinase gene expression in chondrocytes and block cartilage resorption" ppt
Ngày tải lên :
09/08/2014, 06:22
... was inhibited and this led to a reduction in synovial hyperplasia and joint swelling with maintenance of joint integrity [31] Similar results were obtained in an autoantibody-mediated murine ... Adenoviral gene transfer of interleukin-1 in combination with oncostatin M induces significant joint damage in a murine model Am J Pathol 2003, 162:1975-1984 Furumai R, Komatsu Y, Nishino N, Khochbin ... (ATCC, USA) were routinely cultured in DMEM (Invitrogen, Paisley, UK) containing 10% fetal bovine serum (Invitrogen), mM glutamine, 100 IU/ml penicillin, and 100 µg/ml streptomycin Serum-free conditions...
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Báo cáo y học: "The 3rd International Meeting on Gene Therapy in Rheumatology and Orthopaedics" pot
Ngày tải lên :
09/08/2014, 07:20
... confirming the remarkable potency of genetically modified dendritic cells as antiarthritic agents in murine, collagen-induced arthritis A variety of transgenes, including interleukin-4 and Fas ligand, ... an intracellular protein, gene transfer has been the technology of choice for enhancing bone healing However, there are now methods for delivering proteins efficiently into cells by attaching ... Pfizer; Targeted Genetics; and TissueGene References Evans CH, Rediske JJ, Abramson SB, Robbins PD: Joint efforts: tackling arthritis using gene therapy First International Meeting on the Gene Therapy...
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Báo cáo y học: "Catechol-O-methyltransferase gene haplotypes in Mexican and Spanish patients with fibromyalgia" docx
Ngày tải lên :
09/08/2014, 10:21
... explains the multifaceted complaints of FM [4] The defining characteristics of FM (namely, widespread pain and allodynia) could be explained by a pathogenesis known as 'sympathetically maintained ... 'sympathetically maintained pain' [5] Naturally occurring sympathetic neurotransmitters are catecholamines known as norepinephrine, epinephrine, and dopamine All three substances act within the central nervous ... inhibition in rats results in a dramatic increase in sensitivity to pain Low COMT activity may increase pain sensitivity by activating beta (2/3)-adrenergic receptors The objective of our investigation...
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Báo cáo y học: "Identification and analysis of unitary pseudogenes: historic and contemporary gene losses in humans and other primates" docx
Ngày tải lên :
09/08/2014, 20:21
... Adam26B Adam3 a disintegrin and metallopeptidase domain 26B a disintegrin and metallopeptidase domain (cyritestin) chr8+:39299218-39358412 Adam5 a disintegrin and metallopeptidase domain chr9-:103136199-103141451 ... ancestor of euarchontoglires (primates and rodents) In addition to pseudogenes in tandem gene families, we identify 76 losses of well-established genes in the human lineage since the common ancestor ... different steps The final result - the unitary pseudogenes and the polymorphic pseudogenes in human - are listed in Tables and See the main text for details MGI, Mouse Genome Informatics OR, olfactory...
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báo cáo khoa học: "Alcohol tolerance and Adh gene frequencies in European and African populations of Drosophila melanogaster" ppt
Ngày tải lên :
09/08/2014, 22:22
... culture vials initiate isofemale lines These lines were used to estimate Adh allelic frequencies For measuring ethanol tolerance, a mass culture was established by pooling the lines For any population, ... at the same place in larvae and adults ENYAJAN (B et al., 1983) It is interesting to find that, in spite of this difference, the physiologies of larvae and adults which are finally under the control ... considered for explaining the latitudinal cline in Adh gene but with poor experimental evidence (V D 1982) O et al (1982) found TT AKESHO , AN ELDEN that, for Australian populations, the latitudinal...
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báo cáo khoa học: " Conservation and Loss of Ribosomal RNA Gene Sites in Diploid and Polyploid Fragaria (Rosaceae)" doc
Ngày tải lên :
11/08/2014, 11:21
... ng/ml in 2xSSC) and pepsin (0.01% in 10 mM HCl), then denatured in 70% formamide for at 80 oC 25S and 5S rDNA probes in 2xSSC, 50% deionized formamide, and 10% dextran sulphate were denatured for ... Diagnostics, Indianapolis, Indiana) The reaction was initially denatured at 94 oC for min, then subjected to 30 cycles of 94 oC for 30 sec, 50 oC for 20 sec, and 72 oC for min, followed by a 10-min 72 ... 94 oC for min, followed by 30 cycles of 94 oC for 30 sec, 55 oC for 20 sec, and 72 oC for min, followed by a 10-min 72 oC final extension Products were purified by ethanol precipitation and labeled...
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