infantum and l major dna oligonucleotides full genome microarray design

... plu3251 plu3252 plu3253 plu3254 - - ? + plu3248 - plu3247 - plu3246 ? plu3242 plu3243 plu3244 plu3245 /I plu3241 TT01 plu3239 + plu3238 ? plu3237 + plu3236 + plu3235 + plu3232 + plu3231 /I plu3228 ... functions (Table 2): basal cellular functions involving the DNA polymerase III ε chain (plu0943 = dnaQ), enolase (plu0913 = eno), and proteins involved in tryptophan metabolism (plu0799 = tnaA; plu0800 ... archival strains of Salmonella enterica serovar Typhimurium LT7 J Bacteriol 2003, 185:2131-2142 Porwollik S, Wong RM, Helm RA, Edwards KK, Calcutt M, Eisenstark A, McClelland M: DNA amplification and...

Ngày tải lên: 14/08/2014, 20:22

... voyages and all kind of adventures that became popular People who liked to reach and explore remote land like Robinson Crusoe in the same-named novel by Daniel Defoe became typical form of people ... periods: Early Enlightenment, Mature Enlightenment, and Late Enlightenment However, in the study, the English literature in the early 18th century or in the early Enlightenment will mostly be concentrated ... enlighten people and insisted on a systematic education for all However, the English Enlighteners had little revolutionary aims in their views and actions The Enlightenment epoch in English literature...

Ngày tải lên: 26/11/2012, 12:02

... The locally delivered IL2 may activate T and natural killer (NK) cells, which could release a secondary wave of cytokines, and activate immune effector cells In animal models, EMD 273063 was able ... molecule (melanoma model), and in SCID mice reconstituted with human lymphokine-activated killer (LAK) cells and bearing human tumor xenografts (neuroblastoma) [5] Interestingly, CD8+ T cells ... peripheral-blood NK cell number and activity, and increased serum levels of the soluble alpha chain of the IL2 receptor complex (sIL2R), which was observed at doses both higher (4.8 mg/m2 per day) and...

Ngày tải lên: 18/06/2014, 15:20

... biết đâm chồi nảy l c Listen to the rhythm of the falling rain Telling me just what a fool I've been I wish that it would go and let me cry in vain And let me be alone again Hãy l ng nghe nhịp điệu ... để khóc vô vọng Và bỏ l i nỗi cô đơn thêm l n Oh, listen to the falling rain Pitter pater, pitter pater Oh, oh, oh, listen to the falling rain Pitter pater, pitter pater Ô, l ng nghe nhịp điệu ... rain Pitter pater, pitter pater Ô, l ng nghe nhịp điệu tiếng mưa rơi Tí tách, tí tách Ô, ô, ô, l ng nghe nhịp điệu tiếng mưa rơi Tí tách, tí tách ...

Ngày tải lên: 03/12/2015, 13:07

... original sample [18], LDL cholesterol was calculated using the Friedewald formula [LDL = TC – HDL – TG/5] (where TG = Triglycerides) The VLDL was also calculated using the Friedewald formula [VLDL ... 19.9 x AGE] is in Table The “bootstrap” analysis is shown in Figures and The HDL, LDL, and VLDL variables were dealt with by imputed values (HDL-1, VLDL-1 and LDL-1) because the loss of information ... primary care controls were collected in the following health centers: Pilas (Seville-Rural), Camas (Seville-Rural), Huerta del Rey (Seville-Urban) and Mérida (Badajoz-Rural) The cases pertaining...

Ngày tải lên: 03/11/2012, 11:34

... wholesalers Miscellaneous durable goods, merchant wholesalers Apparel, fabrics, and notions, merchant wholesalers Wholesale electronic markets, agents and brokers Professional and commercial equipment ... total of 4.7 million employees, and employed at least 3.5 million people Small businesses make up nearly a third of private-sector employment 2007 SBO Firms with employees at date of survey All ... Immigrant Small Business Owners And, small businesses with half or more of the ownership foreign-born employed at least 3.5 million people, and a likely estimate is 4.7 million (again applying the...

Ngày tải lên: 18/02/2014, 00:20

... will be located either symmetrically (left image), or asymmetrically (right image) [14,15] Colour assignments are as follows: magenta, nucleosome core particle; blue, 146 bp of DNA; red, globular ... structural and biochemical properties of a cultured cell (and hence PTM patterns), particularly when the cells are grown on a monolayer 2D medium Normal cells grown in such a medium can display a nuclear ... differentiation and is most abundant in terminally differentiated cells, there may well be a correlation between Na-terminus acetylation and differentiation Not all methionines are cotranslationally cleaved...

Ngày tải lên: 18/02/2014, 08:20

... markedly hampered receptor glycosylation at the Golgi level resulting in reduced levels of fully glycosylated receptors at the cell surface of transfected cells Reversal of this situation following ... showed very little overlap (not shown) Subcellular distribution of wild-type and mutant FGFR3 molecules To determine more precisely the subcellular localization of the mutant receptors, cells were ... receptor internalization, allowing ligand-independent prolonged signalling to target molecules Mechanisms that control receptor endocytosis are multiple and complex [45] Ubiquitylation is considered...

Ngày tải lên: 19/02/2014, 00:20

... induction Fig Typical trace of experimental chlorophyll (Chl) a uorescence rise form O to P in isolated thylakoid membranes (open circles) and its simulation (full line) by three exponential components ... with a cylindrical hole 25 mm in diameter was attached to the thin copper plate and used as a sample well Before FI measurements, thylakoids were diluted to 50 lgặmL)1 in a total volume of mL in ... granal PQ pool and a slow stromal PQ pool, respectively [8,14,15] However, Joliot et al [34] found half-reduction times, under saturating light, of 2560 ms for the fast pool and 0.81 s for the slow...

Ngày tải lên: 19/02/2014, 05:20

... cleavage or cleavage was relatively unchanged Figure 5A shows that the overall cleavage prole changes gradually with Mg2+, a trend that appears to hold for most nucleotide positions (solid and ... partial alkaline hydrolysis of S RNA; and M, 5Â end-labeled DNA size markers The composite gure is from 8% (upper) and 12% (lower) polyacrylamide gels (B) Phosphorimager scans of Fe2+-EDTA cleavage ... mm) and gel-loading solution For gel markers, end-labeled InDGb RNA (24 ã 105 c.p.m.) was digested with RNases U2, T1, Phy M, or CL3, cleaved with alkali, and cleaved with Mn2+ (3 mm MnCl2, 0.2...

Ngày tải lên: 19/02/2014, 07:20

... (parental cells CCL16, American Type Culture Collection) The V79-G8, V79G18 and V79-G35 cells were from a different parental cell line, V79 (CCL93, American Type Culture Collection) V79-G7 cells are ... and 10 mM MgCl2) at a density of · 107 cellsÆmL)1 Cells were permeabilized by digitonin (100 lgÆmL)1) for  at °C, the cell suspension was diluted 10 fold with HSM buffer, and the cells were harvested ... complex I Experimental procedures Cell lines and cell culture The isolation and preliminary biochemical and genetic characterization of a series of respiration-deficient Chinese hamster mutant cell...

Ngày tải lên: 19/02/2014, 16:20

... thaliana Arabidopsis thaliana Archaeoglobus fulgidus Aspergillus fumigatus Emericella nidulans Bacillus halodurans Geobacillus stearothermophilus Blumeria graminis Burkholderia cepacia Burkholderia ... used for all profile alignments were: gap opening penalty, 10.00; gap extension penalty, 0.1; Blosum 30 protein–weight matrix; helix and strand gap penalty, 4; and loop gap penalty, Finally, areas ... Gloeobacter violaceus Glycine max Gossypium hirsutum Haloarcula marismortui Halobacterium salinarum Legionella pneumophila Lycopersicon esculentum Mesembryanthemum crystallinum Mesorhizobium loti...

Ngày tải lên: 19/02/2014, 16:20

... Del allele (with extra Ig-b gene) and the region II deletion allele (II Del); 6, 16 kb Del allele (with extra Ig-b gene) and the region III deletion allele (III Del) (B) Lanes: and 2, 16 kb Del ... wild type DT40 ⁄ Cre allele; 2, each DHS deletion allele with Bsr gene; 3, each DHS deletion allele without Bsr gene a, DNA from wild type DT40 ⁄ Cre cells; b, DNA from cells with 16 kb Del allele ... from: 1, wild type DT40 ⁄ Cre cells (Wild type); 2, 16 kb Del allele (with extra Ig-b gene) (Control 1); 3, 16 kb Del allele (with extra Ig-b gene) and the region I deletion allele (I Del); and 5,...

Ngày tải lên: 19/02/2014, 16:20

... previously [14] Cells and cell lines Blood samples from common LW and Rh phenotypes (ICAM-4 positive red cells) were obtained from normal volunteers using heparin as an anticoagulant and the LW(a–, ... until further used The L9 29 mouse fibroblast cell line was maintained in IMDM medium supplemented with 10% fetal bovine serum, 100 UÆmL)1 penicillin, and 100 lgÆmL)1 streptomycin The full- length ... quantitated by counting cells in four randomly chosen fields from duplicate wells To remove nonadherent cells, the wells were gently filled with binding buffer, and the microplate was placed floating upside...

Ngày tải lên: 20/02/2014, 11:20

... plastocyanin wild-type as well as five charge mutants MATERIALS AND METHODS Molecular biology and mutagenesis Molecular biological methods were essentially as described by Schlarb-Ridley et al ... on plastocyanin have still to be identified ACKNOWLEDGEMENTS We are extremely grateful to Alexis Balme for his expert help and ¨ advice, to Orjan Hansson, Wolfgang Haehnel, Hualing Mi, William ... to better fits and more reliable k¥ values (Table in [1] and in this paper) Although the curves are shallow, making extrapolation more difficult, and the number of datapoints is smaller than in the...

Ngày tải lên: 21/02/2014, 01:21

... example, Bacillus subtilis and Bordetella pertussis, namely ResA, ResB, ResC and CcdA [12,13] (Fig 1) Notably, the genomes of some Bordetella species, e.g B parapertussis, encode both Systems I and ... (Invitrogen, Paisley, UK) was used for routine molecular biology KOD polymerase (Merck Chemicals Ltd, Nottingham, UK) was used for PCRs All oligonucleotides used in this study are listed in Table S1 (see ... gels), followed by heme staining [48], which stains proteins with covalently bound heme Samples were normalized for wet cell weight, and equal amounts of protein were loaded per lane (5–10 lg)...

Ngày tải lên: 06/03/2014, 09:22

... plants germinate normally, but grow slightly smaller, paler and flower slightly later than wild type [5] However, in our growth chambers, DG plants display a less pronounced phenotype In total, ... PSI particles, samples containing 0.5 and lg chlorophyll were analysed Isolation of thylakoid membranes and PSI particles from Arabidopsis Healthy leaves from typically 5–10 Arabidopsis plants were ... PSI will pave the way for determining the polypeptide’s location within the complex by means of immunogold electron microscopy and single-particle analyses It will also provide a useful orientation...

Ngày tải lên: 07/03/2014, 21:20

... after labelling the cells with the nuclear stain Hoechst 33258 and visualization by ¯uorescence microscopy as described previously [23] Brie¯y, PC12 cells were plated in six-well plastic culture ... and the cells were subsequently stained with Hoechst 33258 (5 lgámL)1) Nuclei that were condensed or fragmented were scored as apoptotic Cell survival assays PC12 cells were plated in 96-well ... In contrast, Leu24-IGF-I, des-(2,3)-Leu24-IGF-I, Leu60-IGF-I and Ala31Leu60-IGF-I (all at nM) failed to prevent serum deprivation-induced cell death, and Ala31-IGF-I and des(2,3)-Ala31-IGF-I exhibited...

Ngày tải lên: 08/03/2014, 22:20

... diversity of cellular functions that this enzyme family facilitates The sequence and functional similarities led us to speculate that plant cell wall-extending expansins and the cyst wall-degrading ... Enzymes and other agents that enhance cell wall extensibility Annu Rev Plant Physiol Plant Mol Biol 50, 391–417 17 Cosgrove, D.J (2000) Loosening of plant cell walls by expansins Nature (London) ... grass group I allergens in timothy grass pollen J Allergy Clin Immunol 92, 789–796 Cosgrove, D.J., Bedinger, P & Durachko, D.M (1997) Group I allergens of grass pollen as cell wall-loosening agents...

Ngày tải lên: 08/03/2014, 22:20