induction of cytochrome p450 enzymes by carotenoids

... indicated, followed by western blotting for Shc, phospho-Erk and total Erk levels (C) Rescue by ectopic Shc isoform expression of Erk activation that was suppressed by down-regulation of endogenous ... Decma-induced Erk activation Activation of Erk by various extracellular signals is often preceded by Shc phosphorylation Accordingly, we examined Shc activation, as indicated by its tyrosine phosphorylation, ... mutation [7], modification of b-catenin [8], or the cleavage of E-cadherin by matrix metalloproteases (MMPs) [9] Cleavage of E-cadherin results not only in the disruption of cell–cell adhesion but...

Ngày tải lên: 19/02/2014, 05:20

... presence of cholesterol allows a closer proximity of the Tyr side chain of the peptide with the terminal methyl group of the acyl chain of the lipid as shown by the observation that the intensity of ... limit of cholesterol [17] Fig Quenching of the fluorescence emission by the NAP-22-peptide (dashed lines) or by the Y11L-NAP-22-peptide (solid lines) of Bodipy-TMR-PI(4,5)P2 LUVs composed of POPC ... Self-quenching of the fluorescence of Bodipy-TMR-PI(4,5)P2 by the MARCKS peptide has been shown to be caused by sequestering of the labeled lipid into domains [12] We show that the quenching of the Bodipy...

Ngày tải lên: 07/03/2014, 17:20

... require specific enzymes, and cytochrome P450 monooxygenases (P450s) make up a significant part of them P450s are heme–thiolate proteins involved in the hydroxylation of a wide range of endogenous ... extracts revealed a clear upregulation of an alkane-inducible cytochrome P450 (AJ273607) The role of P450 in microbial fatty acid metabolism during the first hours of incubation [22] According to amino ... further modified by, for example, several hydroxylation steps (Figs and 4) These hydroxylation steps are very often mediated by cytochrome P450 monooxygenases Discussing biosynthesis of all microbial...

Ngày tải lên: 22/03/2014, 16:21

... reduction of O2 to H2O2 by the reduced heme-containing films as per Eqn (1) and Eqn (2): P450 FeII þ O2 ! P450 FeII À O2 ð1Þ e P450 FeII À O2 þ 2Hþ þ 2 ! P450 FeII þ H2 O2 ð2Þ Fig The effect of CO ... Deisenhofer, J (1993) Crystal structure of hemoprotein domain of P450BM-3, a prototype for microsomal P450s Science 261, 731–736 Guengerich, F.P (2001) Common and uncommon cytochrome P450 reactions ... electrochemistry of the wild-type cytochrome P450BM3 holoenzyme and its isolated FAD-reductase and heme domains Materials and methods Enzymes and chemicals Wild-type cytochrome P450BM3 was overexpressed...

Ngày tải lên: 23/03/2014, 21:20

... can be blocked by antioxidants, it appears that the generation of ROS may be involved in the induction of PAI-1 expression by TNFa via the activation of NF-jB Results Upregulation of PAI-1 expression ... TNFa in the presence or absence of NAC NF-jB promoter activation by TNFa was inhibited by antioxidant, NAC (Fig 3D) To confirm the role of fragment ()664 to )680) of PAI-1 promoter in this reaction, ... Expression of PAI-1 mRNA determined by real-time PCR and (B) activity of PAI-1 promoter (C) Treatment of endothelial cells with the oligonucleotide antisense to RelA p65 abolished formation of the...

Ngày tải lên: 30/03/2014, 11:20

... (2009) The cytochrome p450 homepage Hum Genomics 4: 59-65 Ning D, Wang H, Zhuang Y (2010) Induction of functional cytochrome P450 and its involvement in degradation of benzoic acid by Phanerochaete ... Keywords: cytochrome P450 monooxygenase; Phlebia brevispora; gene cloning; real-time RT-PCR; dioxins; CYP63A Introduction Cytochrome P450 enzymes (CYPs) constitute a large superfamily of heme-containing ... (2009) Metabolism of hydroxylated PCB congeners by cloned laccase isoforms Appl Microbiol Biotechnol 82: 853-860 Han MJ, Choi HT, Song HG (2004) Degradation of phenanthrene by Trametes versicolor...

Ngày tải lên: 20/06/2014, 20:20

... (2009) The cytochrome p450 homepage Hum Genomics 4: 59-65 Ning D, Wang H, Zhuang Y (2010) Induction of functional cytochrome P450 and its involvement in degradation of benzoic acid by Phanerochaete ... Keywords: cytochrome P450 monooxygenase; Phlebia brevispora; gene cloning; real-time RT-PCR; dioxins; CYP63A Introduction Cytochrome P450 enzymes (CYPs) constitute a large superfamily of heme-containing ... (2009) Metabolism of hydroxylated PCB congeners by cloned laccase isoforms Appl Microbiol Biotechnol 82: 853-860 Han MJ, Choi HT, Song HG (2004) Degradation of phenanthrene by Trametes versicolor...

Ngày tải lên: 21/06/2014, 19:20

... SB203580 were used The conversion of DHEA into 7α-OH-DHEA is catalyzed by cytochrome p450 enzyme 7b (Cyp7b) [8] Because of the reported immunostimulating effects of 7α-OH-DHEA, we previously investigated ... RA through induction of transcription of proinflammatory genes [15] NF-κB is retained in the cytosol of nonstimulated cells by a noncovalent interaction with IκB Upon stimulation by TNFα, IκB ... amount of 7α-OHDHEA is expressed as the percentage of 3H-labelled 7α-OHDHEA of the total amount of 3H-label measured Recoveries after extraction were in the range 85–95%, and identification of 7α-OH-DHEA...

Ngày tải lên: 09/08/2014, 07:20

... (latent form of MMP2) and 66 kD (active form of MMP2) on the Coomassie blue background of the gel were considered positive for the presence of enzymatic activity Wu et al Journal of Biomedical ... affected by repression of HSPA4 or HSPA14 in H1299NBS1 cells and the correlation of HSF4b expression with HSPA4/HSPA14 expression (A) & (B) Gelatin zymography of the conditioned medium of H1299NBS1 ... RT-PCR analysis of H1299 cells with knockdown of HSPA4, HSPA14, or both (B) The in vitro migration and invasion activity of H1299 cells with knockdown of HSPA4, HSPA14, or both List of abbreviations...

Ngày tải lên: 10/08/2014, 05:21

... the effect of increased lopinavir clearance secondary to CPY3A4 induction by phenytoin was not offset by the presence of low dose ritonavir used as a “boosting” agent The second arm of participants ... first arm of 12 participants, the addition of phenytoin reduced the area under the concentration-time curve (AUC) of lopinavir and ritonavir by 33% and 28%, respectively after 12 days of overlap ... lack of efficacy, including loss of seizure control or inadequate control of neuropathic pain In many parts of the world where HIV is highly prevalent, such as sub-Saharan Africa and parts of Asia,...

Ngày tải lên: 10/08/2014, 05:22

... formation was deeply characterized by the WR strain of vaccinia virus Induction of cell-cell fusion by VACV-WR can be achieved either directly by infection with high amount of virions per cell or several ... formation of A56 and K2 by ECTV A) Expression of A56 and K2 by VACV-WR and ECTV α-VACV-WR antibody was used as control to viral-proteins expression B) Amino-acids sequence alignment of A56 of VACVWR, ... release from the cell by enzymatic activity of the cellular kinases Src and Abl [9,10] However, disruption of actin by cytochalasineD (Fig 3C) or inhibition of actin tail formation by specific src-kinase...

Ngày tải lên: 12/08/2014, 04:20

... of the bands were determined by the Scion Image software and plotted using the Prism program We thank Dr W S El-Deiry of the University of Pennsylvania, Dr K Zhao of the National Institutes of ... consequence of premature activation of APC/C and inactivation of SCFSkp2 by Tax By contrast, even though the Tax-induced increase in p21 and p27 correlated with APC/C activation, the up-regulation of ... region) previously shown to mediate the induction of p21 expression by tumor growth factor β (TGF-β) [41] Trans-activation of these regulatory elements by Tax and by Sp1 are additive Our data suggest...

Ngày tải lên: 12/08/2014, 23:20

... similarly treated upon infection by the HTLV-I gp46-pseudotyped virions The infection of PBMCs by pseudotyped virions was increased by 1.8 fold in the presence of μM of galectin-1 (Figure 5C) The ... provided detailed analysis on the mechanism of Taxmediated activation of NF-κB by its association to IKK and upstream kinases [18] Modulation of cellular genes by Tax has been extensively studied and ... was then analyzed by RT-PCR As shown in Figure 2A, transfection of the K30 proviral DNA led to an induction in the expression of galectin-1 In addition, comparable induced levels of galectin-1 mRNA...

Ngày tải lên: 13/08/2014, 05:21

... Differential induction of IL-12p70 by CD44hi and CD44lo CD8 T cells 148 Fig 6.6 Expression of CD40L by CD44hi and CD44lo CD8 T cells 150 Fig 6.7 Differential production of IFN-γ, GM-CSF and TNF-α by ... to their Fcε receptors Histamine has a profound effect on DC by suppression of IL-12 and promotion of IL-10 production by DCs, resulting in the induction of Th2 responses (Caron et al., 2001; Mazzoni ... for the induction of DC IL-12p70 production 120 Isolation of pDCs and cDCs from CD11c MACS cell preparations 126 Activation of pDCs by CpG and CD8 T cells 129 Contribution of pDCs...

Ngày tải lên: 11/09/2015, 09:11

... (FL) [82] 2) by recruitment of DC in situ by Flt-3L or chemokines/chemokine receptors 3) by enhancing vaccine capturing and processing by DC 4) by promoting DC maturation and 5) by targeting ... quantitation of DNA 68 2.23 Electrophoresis of DNA and PCR Product 68 2.24 Immunofluorescence staining for confocol microscopy 68 2.25 Determination of protein mass by Western Blot 69 2.26 Collection of ... 3.1.6 Production of cytokines 85 41 xii 3.1.7 Kinetics of induction of DC via in vivo immunization with pNGVL3-hFLex plasmid DNA 88 3.1.8 Comparison of the cell surface phenotypes of purified BM-DC...

Ngày tải lên: 16/09/2015, 15:54

... 4.2 Induction of SULT1A3 by ligands of GR and AhR 61 4.3 Regulation of SULT1A3 by glucocorticoids via GR .63 4.4 Regulation of SULT1A3 via AhR 65 4.5 Effects of other nuclear ... contrast, induction of human SULT1A3 by nuclear receptors has not been well studied Thus, in this study, we systematically examined the induction of human SULT1A3 genes by a whole range of nuclear ... The function of PPARs is modified by the precise shape of their ligand-binding domain induced by ligand binding and by a number of coactivator and corepressor proteins, the presence of which can...

Ngày tải lên: 22/10/2015, 22:37

... (2003) Epoxidation of olens by hydroperoxo-ferric cytochrome P450 J Am Chem Soc 125, 34063407 139 Vaz, A.D.N., McGinnity, D.F & Coon, M.J (1998) Epoxidation of olens by cytochrome P450: evidence ... Hydroxylation by the hydroperoxy-iron species in cytochrome P450 enzymes J Am Chem Soc 126, 115126 141 Vatsis, K.P & Coon, M.J (2002) Ipso-Substitution by cytochrome P450 with conversion of p-hydroxybenzene ... Makris, T.M., Kofman, V., Werst, D.E., Sligar, S.G & Homan, B.M (2001) Hydroxylation of camphor by reduced oxy -cytochrome P450cam: mechanistic implications of EPR and ENDOR studies of catalytic...

Ngày tải lên: 19/02/2014, 16:20

... with bacterial cytochromes P450 [10] The degree of amino acid sequence homology between cytochromes P450 of humans and other species ranges from 48 to 71%, resulting in the prediction of domains ... Both expressed enzymes had no lyase activity towards PROG or 17-OHPROG Understanding of the complexity of hydroxylase and lyase activity of CYP17 by way of primary sequence alignments of CYP17 from ... catalysed by human cytochrome P450 17a-hydroxylase J Clin Endocrin Metab 77, 98–102 Jefcoate, C.R (1986) Cytochrome P-450 enzymes in sterol biosynthesis and metabolism In Cytochrome P450: Structure,...

Ngày tải lên: 08/03/2014, 09:20

... levels of KLF4 were determined by real-time PCR (B) Protein levels of KLF4 were determined by western blot (C) mRNA levels of hSR-BI were determined by real-time PCR (D) Protein levels of hSR-BI ... indicated the effectiveness of stimulus and normal cell status Interestingly, we found that induction of KLF4 by HDL3 could further induce the expression of SR-BI A variety of stimuli have been demonstrated ... increasing expression of KLF4 in PBMC and THP-1 cells, and identified that induction of KLF4 by HDL3 promoted the expression of hSR-BI It has been shown that disruption of SR-BI in mice impairs...

Ngày tải lên: 18/02/2014, 04:20

... capacity of a dominant-negative form of ESE-1 to diminish COX-2 promoter induction in response to LPS or IL-1 exposure Results COX-2 induction by pro-inflammatory stimuli correlates with ESE-1 induction ... stimulation of human monocytic THP1 cells leads to an induction of ESE-1 mRNA expression within h of exposure, reaching a peak at h and leveling off after 24 h [12] To investigate the level of ESE-1 ... role in induction of the COX-2 gene by pro-inflammatory stimuli Discussion The regulation of COX-2 expression during inflammation has been the focus of numerous studies The heterogeneity of parameters...

Ngày tải lên: 19/02/2014, 17:20