induced pluripotential stem cells and the prospects for cardiac cell therapy

... from the pig’s thigh region, and used lentiviruses to induce the cells to pluripotent cells These pluripotent cells are studied and characterized The pluripotent stem cells exhibited morphology and ... The cells were grown in a MEF layer on glass cover slips The media was first aspirated from the cells, and the cells were washed with 1x PBS The cells were then fixed in 4% PFA (EMS, 15710) for ... 25200-072) was then added to the cells and placed in the incubator for minutes, 37oC for the enzyme to take effect, and it was visibly observed that the cells have lifted off the surface of the culture...

Ngày tải lên: 30/09/2015, 10:13

... fat cells, various brain and nervous system cells, insulin-producing cells of the pancreas, bone cells, hematopoietic cells, yolk sac, endothelial cells, primitive endodermal cells, and smooth and ... undifferentiated stem cells for research and therapy To obtain undifferentiated stem cells for research and therapy To obtain undifferentiated stem cells that are genetically matched to recipient for research ... to stem cells In the first, stem cells themselves are implanted in a diseased or injured organ in the hope that they will give rise to the mature cells needed by that organ In the second, the stem...

Ngày tải lên: 15/03/2014, 09:20

... human embryonic blood stem cells Blood 96, 1740 - 1747 IItskovitz - Eldor, J and et al (2000).; Differentiation of human embryonic stem cells into embryoid bodies comprising the three embryonic ... al (1998); Embryonic stem cells lines derived from human blastocysts Science 282, 1145 - 1147 12 Raisman, G., (2001); Olfactory ensheathing cells - another miracle cure for spinal cord injury? ... B - cell differentiation from a human pancreatic cell line in vitro and in vivo Mol Endocrinol 15, 476 - 483 Dzierzak, E et al (1998); Embryonic beginnings of definitive hematopoietic stem cells...

Ngày tải lên: 02/04/2014, 22:20

... scenario, not just for the parties mentioned, but for the recipients as well? Along the same lines, what are the prospects for long-term sustainability of these projects, and what can be done ... A further concern is the sustainability of such projects: if they not prove to be profitable for the private partners, will they abandon the projects? Alternately, the projects provide for sufficient ... nonetheless, that the one billion for whom ICTs are not a priority (those at the bottom of the ladder) and the one billion that the industry is looking to as their next consumers, are not one and...

Ngày tải lên: 21/08/2014, 15:25

... cells via the process of pluripotential reprogramming in which the lineage restriction of differentiated cells is undone The resultant cells, known colloquially as induced pluripotent stem cells ... embryonic stem cells (ESCs) and pluripotent cells of the early embryo, thus gaining their characteristic developmental potential and other characteristics diagnostic of pluripotent cells Conferral ... our body, these cells essentially originate from a common precursor cell within the early developing embryo These versatile cells, known as embryonic stem cells (ESCs), are derived from the epiblast...

Ngày tải lên: 09/09/2015, 18:56

... Teitell MA, Pelegrini M, Plath K, Lowry WE: Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures Cell Stem Cell 2009, 5:111-123 93 Chin MH, Pellegrini ... MH, Pellegrini M, Plath K, Lowry WE: Molecular analyses of human induced pluripotent stem cells and embryonic stem cells Cell Stem Cell 2010, 7:263-269 94 Deng J, Shoemaker R, Xie B, Gore A, LeProust ... Rao A: Tet1 and Tet2 regulate 5-hydroxymethylcytosine production and cell lineage specification in mouse embryonic stem cells Cell Stem Cell 2011, 8:200-213 76 Kriaucionis S, Heintz N: The nuclear...

Ngày tải lên: 11/08/2014, 12:21

... embryonic stem cells Cell Stem Cell 2008, 3:649-657 52 Di Giorgio FP, Boulting GL, Bobrowicz S, Eggan KC: Human embryonic stem cell- derived motor neurons are sensitive to the toxic effect of glial cells ... Gage FH, Goldstein LS, Carson CT: Cell- surface marker signatures for the isolation of neural stem cells, glia and neurons derived from human pluripotent stem cells PLoS One 2011, 6:e17540 48 Funato ... expanding the biopsy into primary fibroblasts, and transducing the cells with retroviruses that encode the transcription factors OCT4, SOX2, KLF4 and cMYC Amazingly, the resultant reprogrammed cell...

Ngày tải lên: 11/08/2014, 12:21

... culture, the medium was refreshed, and then the medium changed every other day Cell passaging was performed when cell confluence reached 85% The purity and immunophenotype of MSCs and their potentials ... of stem cell transplantation, committed stem cells are isolated and purified, and single lineage stem cell transplantation is then performed Deng et al applied intravenous infusion of MSCs in the ... circulation in the necrotic femoral head and surrounding tissues [4] Mesenchymal stem cells (MSCs) are multipotent stem cells that can differentiate into a variety of cell types In the field of cell transplantation,...

Ngày tải lên: 25/10/2012, 11:18

... mesenchymal stem cells) , and for CD29 (β1 integrin) The cells stained poorly for LNGFR and STRO-1 (markers on freshly isolated bone marrow stem cells) and sparsely for 3G5 (marker for vascular ... Isolation, culture and cell surface epitope characterisation of IPFP cells The cells isolated from the IPFP proliferated in culture and reached confluence by day 14 The yield of cells by the end of passage ... cytometry of the IPFP cell population showed a similar staining pattern to that of bone marrow-derived stem cells, and although they stained poorly for STRO-1 and for LNGFR, the expression of these...

Ngày tải lên: 09/08/2014, 10:20

... expanded with and without FGF-2 stained strongly for CD13, CD44, CD90, and CD105 (markers for mesenchymal stem cells) and for CD29 (β1 integrin) (Figure 3) The cells stained poorly for LNGFR and ... (endothelial cell marker) stained endothelial cells in the tunica intima (Figure 1c,d) and anti-αSMA (smooth muscle cell marker) stained cells in the tunica media, forming the muscular wall of the ... were therefore localised to cells in different regions of the small arterioles The positive staining for 3G5 in the perivascular cells suggested the presence of pericytes in the IPFP tissue Cell...

Ngày tải lên: 09/08/2014, 10:23

... Increase in separate culture cells (AD-MSC) and annulus cells comderived mesenchymal stem cells (AD-MSC) and annulus cells compared with separate culture of AD-MSC and annulus cells alone Data from ... the cells gradually spread out and assumed the fibroblastic morphology previously reported for stem cells [32] Cells became confluent after approximately one week With sequential passaging, the ... density of 1000 cells/ mm2 was chosen as a high enough density for cell to cell contact but low enough to allow space for several days of proliferation without the cells becoming confluent Cells were...

Ngày tải lên: 09/08/2014, 10:23

... cells are positive for fibroblast markers (CD90, CD29, and VCAM-1) and no transduced cells were positive for the macrophage marker CD11b Ten percent of the transduced cells are positive for the ... in 5% CO2 for hour in order to separate adherent cells from nonadherent cells The cells in the adherent cell fraction consisting mainly of macrophages are termed antigen-presenting cells (APCs) ... on the intensity of staining in the cartilage Infiltration of cells was scored on a scale of to (0 = no cells, = mild cellularity, = moderate cellularity, = maximal cellularity), depending on the...

Ngày tải lên: 12/08/2014, 12:20

... stem cells to repair cardiac tissues was based on the hypothesis that these cells could differentiate into cardiomyocytes and supporting cell types to replace cells lost during MI/R injury, and ... Mesenchymal stem cells and their potential as cardiac therapeutics Circ Res 95, 9–20 Quesenberry, P.J., Aliotta, J.M., 2008 The paradoxical dynamism of marrow stem cells: considerations of stem cells, ... Stem Cells In The Treatment Of Acute Myocardial Infarction With the emergence of stem cells as potential regenerative medicine, attempts to use stem cells to reduce infarct size and enhance cardiac...

Ngày tải lên: 10/09/2015, 08:42

... integrity, and availability; and you may experience attacks against these dimensions We can think of these as the “primary colors” of information assurance By mixing and matching these and we mix and ... are added to the system • The system typically sits exposed for days before being patched • Can we actually expect > 100,000 systems will be reformatted and reloaded? Vulnerable systems have been ... compromising that system Then the vulnerable host that got compromised can be used to attack other systems, either in the same facility or in other organizations This is one reason for the statement...

Ngày tải lên: 09/12/2013, 17:15

... (UNDP and others) What is needed, now and later? International support, cooperation and coordination Current international support to Viet Nam on CC • Support to the mitigation of effects of CC and ... • In-depth consultation with key stakeholders and the wider public • Refine the CCA strategy • Adopt CC impact scenarios as the basis for public and private investments • Mainstream reduction ... strategies and plans for 2011 and beyond; in public investment decisions; and policy instruments such as SEA • Articulate VN policies, strategies and plans on vulnerability reduction in the context...

Ngày tải lên: 24/01/2014, 00:20

... four fundamental forces One of these is the gravitational force The other three are the electromagnetic force, the weak force, and the strong Force Force particle Mass force Strong Gluon Electromagnetic ... between the two presidents and that therefore, without further ado, the light it emitted traveled the same distance to reach each of them Since the speed of the emitted light to the left and to the ... is for the president of Backwardland, and since the speed of light toward each is the same, the light will reach the president of Forwardland first This is why the citizens of Forwardland claim...

Ngày tải lên: 25/01/2014, 16:20

... market interest rates and accommodating the demand for outside money Changes in these interest rates alter the opportunity costs of money holdings and thereby affect the demand for broad money however, ... to alleviate the funding risks faced by the banks It should therefore facilitate the provision of money and credit to the economy.22 Understanding the funding models of banks and the interlinkages ... borrower and on the value of collateral that the bank receives For instance, a positive shock to the value of the collateral that is pledged to banks implies a lower risk for the bank and thus the...

Ngày tải lên: 16/02/2014, 11:20

... reconcile the differences between the two plans and combine them into a unified plan Congress settled the jurisdiction of the two agencies: the Corps would build and operate the mainstem dams and the ... science and technology and to their use for the general welfare Upon the authority of the charter granted to it by the Congress in 1863, the Academy has a mandate that requires it to advise the federal ... Sciences, the National Academy of Engineering, and the Institute of Medicine The members of the committee responsible for the report were chosen for their special competences and with regard for appropriate...

Ngày tải lên: 17/02/2014, 19:20

... PADL From the left hand side panel in Table it is clear that non ADL and IADL cells for both genders above 85 years old are too small for reliable estimates of the mean For the PADL cells the number ... receiving some help, the sources of the help was asked The list covered both informal and formal care-givers and respondents were asked to list all of them and name one who helps the most The structure ... costs and the expenses of the social and health services are increasing The report analyses the factors influencing the use of social and health services by the elderly On the basis of the analysis,...

Ngày tải lên: 14/03/2014, 17:20

... WHA55.25) The Regional Office will publish the research papers later this year This publication outlines the extent of the problem, the implications for the health sector and other sectors, and the ... National Institute for Public Health and the Environment, Bilthoven, Netherlands Rudolf Hoogenveen National Institute for Public Health and the Environment, Bilthoven, Netherlands Alan A Jackson ... The dynamics of the food system, particularly in the expanding markets of eastern Europe, needs further exploration and the evolution of the food supply, particularly the price and availability...

Ngày tải lên: 16/03/2014, 14:20