induced pluripotent stem cell production and characterization an overview of somatic cell reprogramming
báo cáo khoa học: " Capturing Alzheimer’s disease genomes with induced pluripotent stem cells: prospects and challenges" pps
Ngày tải lên :
11/08/2014, 12:21
... R, Loring JF: Dynamic changes in the copy number of pluripotency and cell proliferation genes in human ESCs and iPSCs during reprogramming and time in culture Cell Stem Cell 2011, 8:106-118 37 ... impor tant contributions to the stem- cell field Differentiation methods such as those described in Pruszak et al [46] and Yuan et al [47] offer an approach to simultaneously quantify and purify cell ... Lavon N, Biancotti J-C, Yakir B, Clark AT, Plath K, Lowry WE, Benvenisty N: Identification and classification of chromosomal aberrations in human induced pluripotent stem cells Cell Stem Cell 2010,...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
Ngày tải lên :
11/08/2014, 12:21
... methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 101 Santenard A, ... combination of stem cell models and epigenomics in studies of the role of non-coding mutations in human disease Epigenomic analyses of cells derived through in vitro stem cell differentiation models can ... structure and gene expression programs of human embryonic and induced pluripotent stem cells Cell Stem Cell 2010, 7:249-257 90 Lister R, Pelizzola M, Kida YS, Hawkins RD, Nery JR, Hon G, AntosiewiczBourget...
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Screen of nuclear receptors for the enhanced and alternative generation of induced pluripotent stem cells
Ngày tải lên :
09/09/2015, 18:56
... restriction of differentiated cells is undone The resultant cells, known colloquially as induced pluripotent stem cells (iPSCs), become akin to pluripotent embryonic stem cells (ESCs) and pluripotent cells ... different cells can also be induced to fuse with one another in vitro For instance, when a somatic cell fuses with a pluripotent cell such as an ESC, the resultant hybrid cell still remains pluripotent ... parthenogenesis of an unfertilised egg is also another means in which pluripotent cells can be derived Parthenogenesis occurs in certain female organisms and involves the asexual reproduction of an embryo,...
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Generation of porcine induced pluripotent stem cells and their differentiation into cardiac lineages
Ngày tải lên :
30/09/2015, 10:13
... abilities of stemcells Hence, NANOG is essential for the maintenance of human Embryonic Stem Cells in vitro The mRNA of NANOG is only present in undifferentiated and pluripotent mouse and human cell ... of cells transitioning from fibroblasts to Induced Pluripotent Stem Cells 45 iv 4.10 Culture of Porcine Induced Pluripotent Stem Cells 45 4.10 Characterization of Porcine Induced Pluripotent ... region, and used lentiviruses to induce the cells to pluripotent cells These pluripotent cells are studied and characterized The pluripotent stem cells exhibited morphology and genomic markers of...
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Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
Ngày tải lên :
19/02/2014, 06:20
... presence of 15 mM L-dopa, as analyzed by oxygen consumption and spectrophotometric assay oxidation of the substrate, presumably because of steric hindrance The presence and the position of an amine ... Stereospecificity and substrate specificity The stereospecificity of T reesei TYR2 was studied by following the activities on 15 mm l-dopa, dl-dopa and d-dopa and 2.5 mm l-dopa, dl-dopa and d-tyrosine, and the ... existence of TYR2 as a double band in SDS ⁄ PAGE, the two protein bands were individually cut out and ‘in-gel’ digested with trypsin Mass fingerprint analysis of the tryptic fragments by MALDI-TOF MS...
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Báo cáo khoa học: Production and characterization of a thermostable L-threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus docx
Ngày tải lên :
16/03/2014, 14:20
... Machielsen and J van der Oost Metals and inhibitors The effect of several salts, metals and inhibitors on the initial activity of Pf-TDH was checked using butane2,3-diol as substrate in the standard ... sequence of the expression clone was confirmed by sequence analysis of both DNA strands Production and purification of ADH E coli BL21(DE3) harbouring pSJS1244 was transformed with pWUR78 and a single ... reaction and acetoin in the reduction reaction The activity of Pf-TDH was significantly increased by the addition of mm CoCl2 (relative activity to that of the standard reaction 170%) and not by...
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Báo cáo khoa học: Production and characterization of a noncytotoxic deletion variant of the Aspergillus fumigatus allergen Aspf1 displaying reduced IgE binding ppt
Ngày tải lên :
16/03/2014, 19:20
... loaded), and (B) immunoblotting (0.1 lg protein) using an anti-Aspf1 antiserum, of Aspf1 (lane 1), and Aspf1D(7–22) (lane 2) The samples were previously reduced with 5% (v ⁄ v) 2-mercaptoethanol and ... immunoreactivity of these two proteins has also been studied Results Production, isolation, and spectroscopic and structural analysis of recombinant proteins The recombinant protein Aspf1 and its deletion ... lgÆmL)1) and chloramphenicol (34 lgÆmL)1) and grown at 37 °C in minimal medium up to an optical density at 600 nm of 0.7 Then, the protein production was induced with mm IPTG and the cells were...
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Báo cáo lâm nghiệp: "Production and characterization of exocellular in ectomycorrhizal fungi proteases" pps
Ngày tải lên :
09/08/2014, 04:20
... As shown in Table I, A rubescens and L subdulcis secreted larger amounts of exocellular proteases than C geophilum and H crustuliniforme, although growth of the former fungi was considerably ... enzymes and their distribution in the soil layers References Abuzinadah R.A & Read D (1986) The role of proteins in the nitrogen nutrition of ectomycorrhizal plants I Utilization of peptides and ... & Induction of extracellular Martin F (1986) proteases in the ascomycete Cenococcum geophilum In: Physiological and Genetical Aspects of Mycorrhizae Proceedings of the Ist European Symposium...
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Báo cáo y học: " Induced pluripotent stem cells free of exogenous reprogramming factors" pot
Ngày tải lên :
14/08/2014, 21:20
... and genetic approach for the generation of induced pluripotent stem cells Cell Stem Cell 2008, 2:525-528 Shi Y, Desponts C, Do JT, Hahm HS, Schöler HR, Ding S: Induction of pluripotent stem cells ... period of time (usually weeks for mouse cells, and 3-4 weeks for human cells) Whether reprogramming by defined factors can be further optimized will depend on a better understanding of the mechanisms ... and clinical applications Reprogramming of somatic cells by forced expression of defined factors is clearly different from reprogramming through somatic cell nuclear transfer or fusion with pluripotent...
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Human pluripotent stem cell derived cellular vehicles for cancer gene therapy
Ngày tải lên :
09/09/2015, 10:08
... Stem cells- unique candidates for therapeutic platform 1.2.1 What are stem cells 1.2.1.1 Pluripotent stem cells 19 20 20 1.2.1.1.1 Embryonic stem cells 20 1.2.1.1.2 Induced pluripotent stem cells ... potency of stem cells 1.2.1.1 Pluripotent stem cells Except for totipotent stem cells consisting of zygotes and their offspring cells from the first few divisions, pluripotent stem cells are the ... model of non-small cell lung cancer (Trang et al., 2011) 1.1.1.6 Anti-angiogenic cancer gene therapy Another potential strategy for cancer treatment is anti-angiogenic therapy The most important and...
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Tài liệu Module 4: Creating and Deploying an Image of Windows 2000 Professional pptx
Ngày tải lên :
17/01/2014, 08:20
... Creating and Deploying an Image of Windows 2000 Professional Introduction to Creating and Deploying an Image Slide Objective Windows 2000 Professional and Applications To introduce how an image ... installation of Windows 2000 Professional Creating an Image Testing an Image Deploying an Image At the end of this module, you will be able to: Explain the role of a reference computer and image, and ... Windows Explorer and folder named Northwind Traders on the desktop Module 4: Creating and Deploying an Image of Windows 2000 Professional Overview Slide Objective To provide an overview of the module...
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Tài liệu An overview of customer satisfaction and loyalty doc
Ngày tải lên :
18/01/2014, 15:20
... Plans Phase 6: Tracking Phase 3: Needs and Performance Evaluation Provide Quantitative Measures of: • Importance of Key Attributes • Company Performance on Key Attributes • Competitive Performance ... quality of products and services to both your employees and your customers • Determine competitive strengths and weaknesses • Gain additional insight into the sources of dissatisfaction and areas ... Expectations of Specific Levels of Performance Determine Areas of Greatest Impact Provide Ongoing Quantitative Assessment of Performance Deliver Easy-toUnderstand Reports to Employees/Management...
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Tài liệu Database Encryption – An Overview of Contemporary Challenges and Design Considerations pdf
Ngày tải lên :
19/02/2014, 12:20
... Proceedings of Data and Applications Security, 19th Annual IFIP WG 11.3 Working Conference, USA [12] Kühn U (2006) Analysis of a Database and Index Encryption Scheme – Problems and Fixes Secure Data Management ... Security and Privacy Symposium, May [16] Bayer R, Metzger JK (1976) On the Encipherment of Search Trees and Random Access Files ACM Trans Database Systems, 1, 37-52 [17] Damiani E, De Capitani diVimercati ... both data security and performance Table summarizes the influence of encryption granularity on several aspects Better performance and preserving the structure of the database cannot be achieved...
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AN OVERVIEW OF NETWORK SECURITY ANALYSIS AND PENETRATION TESTING docx
Ngày tải lên :
05/03/2014, 21:20
... analysis to assess firewall and filter rulesets and identify the Operating Systems of the target hosts via TCP/IP fingerprinting techniques Standard connect() TCP scanning Vanilla TCP portscanning ... Spoofed portscanning A new breed of publicly available scanner is spoofscan.c by jsbach, which is available from http://packetstorm.securify.com/UNIX/scanners/spoofscan.c Spoofscan takes advantage ... Host and jsbach’s spoofscan utility installed Due to the fact that spoofscan sends out spoofed probe packets and then sniffs the responses using the shared network segment, we can spoof portscans...
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Guidance for Planning and Conducting an Audit of Internal Controls of Public Debt docx
Ngày tải lên :
06/03/2014, 23:20
... Committee Guidance For Planning And Conducting an Audit of Internal Controls of Public Debt S ecction Introduction The tasks of understanding debt’s fiscal and economic interrelationships and responding ... budget office and central bank, and the extent of SAI’s authority over debt matters SAIs should review their countries’ legal framework and actual debt procedures to gain an understanding of the ... relationships with creditors and underwriters; and establishing control systems to collect, measure, and report on debt transactions and levels and their associated risks Some of the steps involved...
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Agent Orange and Cancer: An Overview for Clinicians doc
Ngày tải lên :
15/03/2014, 01:20
... association of dioxin exposure with lung cancer Prostate Cancer While the VA19 and Ranch Hand62 studies did not show an excess of prostate cancer, the Australian veterans study (AIHW) did show an excess, ... exposure, and brain cancer.12 Other Cancers Available evidence does not permit a conclusion regarding an association between Agent Orange exposure and other cancers, including cancers of the nose and ... ASSOCIATION OF AGENT ORANGE AND CANCER Human Evidence Epidemiologic studies of Vietnam veterans potentially provide the most direct evidence of CA Cancer J Clin 2003;53:245–255 the health effects of Agent...
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Blood Cell – An Overview of Studies in Hematology pptx
Ngày tải lên :
16/03/2014, 21:20
... transporting other proteins and molecules into and out of the cell, maintaining cell structure, attaching to other cells and molecules, and participating in chemical reactions [1] Those systems ... pathophysiology and genetics of hematological disorders and malignancies, blood testing and typing, and the processes governing hematopoiesis Blood cell physiology, biochemistry and blood flow ... the dynamics of RBC’s antigens Rediscovering Red Blood Cells: Revealing Their Dynamic Antigens Store and Its Role in Health and Disease 23 The role of RBC antigens transport in health and disease...
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An Overview of Consumer Data and Credit Reporting ppt
Ngày tải lên :
22/03/2014, 18:20
... includes national oil and gas companies, travel and entertainment companies, utility companies, real estate firms, government entities, and smaller retailers ing loans and any evidence of money-related ... indicators of credit use and repayment performance, including measures of credit utilization, numbers of recently opened accounts, and timing and severity of payment problems The breadth and timeliness ... categories: banking institutions (commercial banks and savings associations), finance companies and credit unions, retailers, and ''other.'' The retail category includes department stores and jewelry,...
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An overview of research, policy and practice in teacher supply and demand, 1994–2008 docx
Ngày tải lên :
22/03/2014, 19:20
... teacher supply and demand through an analysis and assessment of the relationship between research, policy and practice on the subject and how they impact on the supply and demand of teachers in ... language of ‘supply and demand’ is the concept of the market The market analogy is implicit in and assumed in discussions of teacher supply and demand, and yet this analogy is often fallacious Within ... conditions and geographies shaped by history These constrain and enable the parameters of choice and decision-making, and they are critical to an analysis of failure to balance supply and demand Here...
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