increased cell proliferation and survival

... required for cell proliferation and cell growth or whether its essential role in proliferation is restricted to specific cell types Control of Cell Proliferation and Growth by Myc Proteins 331 ... targets of Myc in cell proliferation and their proposed functions Control of Cell Proliferation and Growth by Myc Proteins 333 2003) There is clear evidence that this effect is direct and not mediated ... both cell growth and cell proliferation through multiple pathways and that there is neither a single critical effector gene nor a small group of such genes In cell culture, Myc can affect cell...

Ngày tải lên: 25/10/2013, 21:20

... LN, cell proliferation and survival are clearly enhanced [13,14] From our results, both proliferation and survival of GC cultured on LN, unlike steroidogenesis, were closely associated with cell ... cytochalasin on GC proliferation rate and cell death In various cell models, such as kidney epithelial cells [49], hepatocytes [50] or carcinoma cells [51], cytochalasin has been shown to induce cell detachment ... numbers, and RGD on subpyknotic anti-α6 different ratios follicles culturedpeptides Effect of6rates ofIgG on cellsmall of LNproliferation and Effect of anti-α6 IgG on cell numbers, proliferation and...

Ngày tải lên: 05/03/2014, 17:20

... the number of bands and the labeling intensity were increased in growing cells The most prominent increases were in bands of 140, 133, 116, 95, 82, 73 and 56 kDa (Fig 4) Other bands also showed ... not PARP-1, was required for SMC proliferation, and identified a novel link between mono-ADP-ribosylation and c-fos gene expression Materials and methods Materials Cell culture materials (DMEM, fetal ... electrophoresis in 1.7% agarose gels and visualized with SYBR Green I Data measurement and statistical analysis Radiotracer, cell number and enzyme assay data were quantified and plotted as means ± SEM...

Ngày tải lên: 17/03/2014, 09:20

... CK2a knockdown increased endogenous p53 and p21 expression and decreased endogenous C-myc expression (Figure 4C) Thus, it can be inferred that the inhibition of cell proliferation and cell cycle ... http://www.translational-medicine.com/content/9/1/97 Cell lines and culture conditions The human colorectal cancer cell lines LoVo, SW480, HT29, HCT116 and LS174T were maintained in RPMI 1640 (Gibco, Grand Island, NY, USA) supplemented ... CRC cell proliferation compared to the control (nonspecific siRNA) (F = 32.854, P < 0.01 for LoVo cells; F = 32.655, P < 0.01 for SW480 cells), and treatment with emodin markedly reduced proliferation...

Ngày tải lên: 18/06/2014, 19:20

... material and immune cell development involves in human carcinogenesis In this study, we studied about the toxic effects of borax on immune cell proliferation (lymphocyte proliferation) and genotoxicity ... the correlation between the immune cell proliferation and the borax cytotoxicity Results Studying the effect of borax on immune cell proliferation (lymphocyte proliferation) The cultures of human ... effectiveness to lymphocyte proliferation in this study It was toxic for cellular proliferation Borax effects lymphocyte proliferation and it may cause cytotoxicity in immune cell (lymphocyte) SCE...

Ngày tải lên: 20/06/2014, 00:20

... FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization Journal of Orthopaedic Surgery and Research 2011 6:8 Submit your next manuscript to BioMed Central and take ... osteoblast-like cells in normal media (NM) consisting of alpha MEM medium with 10% fetal calf serum (FCS), 1% antibiotic solution and 1% glutamine solution and subcultured the cells every to days The cells ... minutes and resuspending at a concentration of × 105 cells/ml For experiments, we grew the cells in the NM above, using multi-well plates After three days, the cells reach confluence and mineralization...

Ngày tải lên: 20/06/2014, 04:20

... FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization Journal of Orthopaedic Surgery and Research 2011 6:8 Submit your next manuscript to BioMed Central and take ... osteoblast-like cells in normal media (NM) consisting of alpha MEM medium with 10% fetal calf serum (FCS), 1% antibiotic solution and 1% glutamine solution and subcultured the cells every to days The cells ... minutes and resuspending at a concentration of × 105 cells/ml For experiments, we grew the cells in the NM above, using multi-well plates After three days, the cells reach confluence and mineralization...

Ngày tải lên: 20/06/2014, 07:20

... both Cdc42 and huntingtin (HD) and shows cell type-specific localization Trip10 was cloned and transfected into (A) IMR-32 cells and (B) CP70 cells; individual colonies were selected and analyzed ... moderately methylated in breast cancer cells (MCF7 and MDA-MB231) and liver cancer cells (HepG2) Heavy methylation was seen in brain tumor cells (IMR-32 and U87) (Figure 1A left, Additional File ... Trip10 exhibited increased cell death [9], suggesting that Trip10 is toxic to striatal neurons These data demonstrate that the function of Trip10 in cell survival and growth is cell lineage-specific...

Ngày tải lên: 10/08/2014, 05:21

... study; and manuscript preparation TM and HK contributed to MSC stimulation, quantitative PCR, and Bio-Plex; CIA induction and evaluation; humoral and cellular responses; and analysis of T -cell proliferation ... plates and grown to confluence Osteogenesis and adipogenesis were induced as described previously [29] and [30], respectively) Anti-CD3-induced cell proliferation CD4 + T cells and accessory cells ... contributed to isolation and characterization of MSCs; MSC stimulation, quantitative PCR, and Bio-Plex; CIA induction and evaluation; humoral and cellular responses; analysis of T -cell proliferation; design...

Ngày tải lên: 12/08/2014, 11:23

... below 2.1.2.1.3 Cell lysis CHO cells were collected and re-suspended in suitable amount of SPHK buffer (described below) Cells were lysed by several cycles of freeze-thawing and total cell lysates ... U937 cells viability 5,000 and 10,000 cells were treated with 25μM of DMS or different compounds for 24 hours or 48 hours Cells were then stained with Trypan blue and counted under microscope Cells ... compounds and DMS were tested for their cytotoxicity in U937 cells U937 cell were incubated with 25μM of compounds or DMS for 24 hours and 48 hours in 96-well plates (5,000 or 10,000 cells/well)...

Ngày tải lên: 11/09/2015, 16:06

... suppressing SPHK activity in the stem cells, the balance between proliferation and differentiation could be lost, the cell growth was arrested, and the cells would then enter into certain differentiation ... PBS, and 200μl of distilled water was added into each well and at least five rounds of freeze-thaw (-80°C and 37°C) were performed to lyse the cells thoroughly Cells were then centrifuged and ... inhibit at all cell growth compared with untreated cells (DMSO=0) 3.2.3 Function of CP6 and DMS in Human BM- and AD- MSCs Differentiation Human BM- and AD- MSCs are multipotent cells that are...

Ngày tải lên: 11/09/2015, 16:06

... stem cells proliferation, what it functioned as: did it function as an extracellular mediator to bind with its receptors and promote cells proliferation? Or it penetrated into the cells and bind ... BM- and AD- MSCs proliferation Human BM- and AD- MSCs were cultured in different culture conditions and cells proliferation were tested by PicoGreen assay Results are the average + the standard ... the stem cells remained as stem cells Starting cells for human BM-MSCs were passage 6, and for human AD-MSCs (cells from two different donors) were passage Cells were equally grouped and cultured...

Ngày tải lên: 11/09/2015, 16:06

... able to induce an excellent level of cell proliferation in serum-reduced media (1% FBS), especially in the human AD-MSCs, where 1μM S1P and 1% FBS could promote around 50% more cells growth (Figure ... accumulated on cell expansion and differentiation might also be applicable in other types of stem cells Also, it should be noticed that both human BMand AD- MSCs are primary cells, and thus they may possess ... differentiations pathways, to further validate and confirm the potential usage of SPHK inhibitors in stem cell differentiation, and S1P roles on stem cell proliferation 157 ...

Ngày tải lên: 11/09/2015, 16:06

... intracellular sphingosine-1-phosphate and promotes cell growth and survival. " J Cell Biol 147(3): 545-58 Olivera, A and S Spiegel (1993) "Sphingosine-1-phosphate as second messenger in cell proliferation ... sphingosine-1-phosphate and platelet-derived growth factor in the maintenance of human embryonic stem cells." Stem Cells 23(10): 1541-8 Peng, H and J Huard (2003) "Stem cells in the treatment of muscle and connective ... "Whole genome analysis of human neural stem cells derived from embryonic stem cells and stem and progenitor cells isolated from fetal tissue." Stem Cells 25(5): 1298-306 Shmelkov, S V., S Meeus,...

Ngày tải lên: 11/09/2015, 16:06

... one hand, it could act as an intracellular mediator and trigger numerous cellular events, including intracellular calcium release, and promote cell survival and cell growth On the other hand, ... messenger and as an extracellular ligand for specific cell- surface receptors to regulate cell survival, in either case S1P has been shown to promote cell proliferation, morphological changes and migration ... S1P in stem cell proliferation and multipotency maintenance It is well known that in stem cells, there is a balance between cell proliferation and differentiation, which directs the cells to proliferate...

Ngày tải lên: 11/09/2015, 16:06

... ECV304 proliferation and PCNA expression To test whether NAMI-A may affect cell proliferation, we first examined the rate of DNA synthesis by following [3H]thymidine incorporation in ECV304 cells ... synthesis in cells cultured under standard conditions This effect was evident after 24 h of treatment and was still consistent in cells exposed to the ruthenium compound for 48 and 72 h, indicating ... promotes cell proliferation, as indicated by the strong correlation between ERK1/2 activation and both DNA synthesis and PCNA expression [22,23] Here, we assessed both ERK1/2 activation and activity...

Ngày tải lên: 21/02/2014, 01:21

... two Ptb) ⁄ ) ES cell lines, and found that Ptb) ⁄ ) ES cells showed severe defects in cell proliferation in vivo and in vitro (Figs 3B and 4B) As Ptb) ⁄ ) ES cells exhibit a low proliferation rate, ... both wild-type cells and the two Ptb) ⁄ ) ES cell clones Scale bar: 100 lm (B) Cell proliferation assay Cells (5 · 104) were seeded (d0) and counted every day for days of culture The proliferation ... undifferentiated state and did not lead to precocious differentiation PTB in development and ES cells 2.5 1.5 0.5 +/+ –/– Fig Ptb) ⁄ ) ES cells have a severe defect in cell proliferation in vivo and in vivo...

Ngày tải lên: 07/03/2014, 00:20

... the proliferation of HeLa (s), and MCF-7 (d) cells Cell proliferation was determined after one cell cycle using the sulforhodamine B assay Error bars indicate SD (B) Rotenone arrested the cell ... apoptotic cells and PI-stained cells (red) indicated late apoptotic ⁄ necrotic cells apoptosis [22] Staining the cells with both annexin V and PI helped to differentiate between the early and late ... microtubule organizations and functions in tumor cell lines, activated mitotic check points, inhibited cell proliferation at mitosis and induced programmed cell death in the arrested cells The apparent...

Ngày tải lên: 07/03/2014, 05:20

... understand the entire organism, and not merely an isolated function This book is devoted to the presentation of a collection of articles on adaptation and survival strategies at the level of cell ... propagation D Remias continues with snow and ice (Chap 13), now as a habitat, and reports on recent findings in the cell physiology of snow and ice algae from the Alps and polar regions The extreme growth ... their studies in order to understand the limits of cellular adaptation and survival strategies Finally I would like to thank the Springer team for their support and valuable suggestions on editing...

Ngày tải lên: 14/03/2014, 09:20