in vivo pharmacokinetics and pharmacodynamics
Doxorubicin conjugated to d alpha tocopheryl polyethylene glycol 1000 succinate (TPGS) in vitro cytotoxicity, in vivo pharmacokinetics and biodistribution
Ngày tải lên :
04/10/2015, 17:04
... precursor drug, such as increased solubility, enhanced bioavailability, improved pharmacokinetics, ability of targeting and protected activity of protein drug (Khandare and Minko 2006) In particular, ... TPGS-DOX conjugate, was investigated on drug loading efficiency, conjugate stability, drug release property, intracellular uptake, in vitro cytotoxicity and in vivo pharmacokinetics and biodistribution ... amphiphilic and can dissolve in both organic solvents and water In addition to linear PEG chains, there are also some branched PEGs in which two or more PEG chains may be joined through linkers such...
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báo cáo hóa học:" Mycophenolate pharmacokinetics and pharmacodynamics in belatacept treated renal allograft recipients – a pilot study" doc
Ngày tải lên :
18/06/2014, 15:20
... regimens included doses of 10 mg/kg administered as a 30 minutes intravenous (iv) infusion Doses were given at day and 5, and at weeks 2, 4, and 12 for both regimens The more intensive regimen included ... by inhibiting IMPDH, and thereby the proliferation of activated lymphocytes [6] MPA demonstrates a narrow therapeutic range and substantial inter- and intraindividual variability of pharmacokinetic ... activity in CD4+ cells, intracellular MPA concentrations were restored by incubating the isolated cells in filtrated plasma originating from the same sample The IMPDH activity was determined in cell...
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Báo cáo khoa học: A study of microRNAs in silico and in vivo: diagnostic and therapeutic applications in cancer pot
Ngày tải lên :
16/03/2014, 01:20
... lymphomagenesis in mice [44] The miRNA miR-21 is overexpressed in many solid tumors, including breast, colon, lung, prostate and stomach, and in endocrine pancreas tumors, glioblastomas and uterine leiomyomas ... of essential proteins responsible for accelerating the cell cycle, including cyclin D and retinoblastoma [56,57] Moreover, alterations in the machinery responsible for processing miRNA contributes ... examined to date [8,52], a feature that could be particularly useful in diagnosing incident cancers in otherwise normal tissues Indeed, this approach discriminates normal and neoplastic tissues in...
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Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre doc
Ngày tải lên :
17/03/2014, 10:20
... are no clashes of amino acid side chains in the core of the structure, and most mutations occur on the protein surface The FAD binding groove is conserved with most ligand binding residues identical ... misfolding of the short Erv1p because FAD binding and in vitro enzyme activity are unchanged Changing C30 or C33 to serine drastically increases the amount of observed dimers Probably, the remaining ... vivo (Tables and 2) provides genetic evidence for an essential involvement of the N-terminal cysteine pair in the in vivo function A possible function of this N-terminal CXXC motif in Erv1p is...
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Báo cáo khoa học: LRRK2 in Parkinson’s disease: in vivo models and approaches for understanding pathogenic roles pdf
Ngày tải lên :
23/03/2014, 04:20
... various model systems, including lower eukaryotes and invertebrates, in order to obtain clues for building important hypotheses The rapidity and efficiency of in vivo studies in many nonmammal models ... understanding pathogenic roles Z Yue function [5] LRRK2 also contains armadillo-like repeats, LRR, kinase and WD40 domains [6] In vitro biochemical analysis demonstrates that LRRK2 contains kinase and ... in the process of searching binding proteins, the in vivo evidence for the relationship between ROCO proteins and cytoskeletons in D discoideum suggests a need to further investigate the possibility...
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Báo cáo khoa học: "In vivo morphological and antigenic characteristics of Photobacterium damselae subsp. piscicida" doc
Ngày tải lên :
07/08/2014, 20:23
... HMW bag in vivo had bands at 27, 34, 39, and 79 kDa Staining of the 27 and 79 kDa bands of bacteria cultured in HMW bag in vivo was particularly intense, as were the bands at 26, 27, and 79 kDa ... piscicida in vivo 173 shown in Fig 3, distorted staining of the 74 kDa band was obtained with whole cell extracts of bacteria cultured in vitro and in vivo, and also with ECP from bacteria cultured in ... bacteria shown in Lane 2, Lane 2: bacteria maintained in vivo in 300 kDa dialysis tubing (HMW bag), Lane 3: concentrated ECP of bacteria shown in Lane 4, Lane 4: bacteria maintained in vivo in 25 kDa...
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Báo cáo y học: "Pharmacokinetics and pharmacodynamics of danaparoid during continuous venovenous hemofiltration: a pilot study" ppt
Ngày tải lên :
13/08/2014, 08:20
... heparin in the past 30 days, and in all patients the platelet count decreased more than 50% within day after rechallenge Two patients suffered from skin necrosis, and in no patient could a definite ... Thrombin generation remained within the normal range during the first hours To our knowledge, this is the first time the pharmacokinetics and pharmacodynamics of danaparoid have been studied during ... grafting Page of (page number not for citation purposes) Critical Care Vol 11 No de Pont et al Figure Pharmacokinetics and pharmacodynamics of danaparoid during continuous infusion after a loading...
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Báo cáo y học: " Identification of unique reciprocal and non reciprocal cross packaging relationships between HIV-1, HIV-2 and SIV reveals an efficient SIV/HIV-2 lentiviral vector system with highly favourable features for in vivo testing and clinical usa
Ngày tải lên :
13/08/2014, 09:21
... coding regions (pSVR∆GP-CMVGFP) and also the generation of a self inactivating (SIN) vector (pSVR∆SIN-CMVGFP) created by additional deletion in the 3' UTR This will be copied into the 5'LTR during ... packaging relationship between SIV and HIV-2 Interestingly, the major packaging determinant of both HIV-2 and SIV has been shown to be upstream of the major splice donor [9,10] and by inference ... encompassing gag and pol sequences) was discarded The remaining fragment was religated and formed pSVR∆GP CMVGFP was obtained from SalI digestion of pSVR∆-CMVGFP and ligated into the SalI linker...
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IN VIVO MECHANICAL AND PHYSIOLOGICAL CHARACTERISATION OF LOWER LIMB SOFT TISSUE BY a LOCAL INDENTATION TECHNIQUE
Ngày tải lên :
09/10/2015, 11:24
... the stump’s soft tissues in contact with the prosthetic socket, liner and socks The main factor in determining comfort of the prosthesis and its effectiveness in restoring the amputee's mobility ... fact that the indentation probe is hand-held makes it difficult to ensure repeatability in the positioning and alignment of the probe Maintaining a constant indentation rate by hand is almost ... fitness by obtaining the pain-pressure threshold and tolerance for a below-knee amputee and combining this information with finite element analysis For residual limbs, the tolerant and sensitive...
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Pharmacokinetics pharmacodynamics driven approach for lead optimization in anti mycobacterial and anti malarial drug discovery
Ngày tải lên :
22/09/2015, 15:17
... arylaminoalcohol, 4-aminoquinolines, 8amionquinolines, sesquiterpene lactone (artemisinin), biguanides, diaminopyrimidines, sulfonamides, hydroxynaphthoquinone, and antibiotics Quinine and quinidine ... capreomycin, aminosalicylic acid, and fluoroquinolones (including ofloxacin, levofloxacin, gatifloxacin and moxifloxacin)], and is commonly administered for years or longer including daily injections ... Diaminopyrimidines Antimalarial drug Quinine (Cinchona alkaloids) Quinidine Mefloquine Halofantrine Benflumetol (Lumefantrine) Chloroquine Amodiaquine Pyronaridine Primaquine Tafenoquine Artemisinin...
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Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
Ngày tải lên :
25/10/2012, 11:10
... characterized by insulin resistance and insulin secretion deficiency At present, there is no a single medication which treats type diabetes by improving both insulin resistance and insulin secretion ... fusion protein exhibited significant glucose-lowering effect in diabetic mice and may be a promising agent that can treat type daibetes by improving both insulin resistance and insulin secretion ... fusion protein in inclusion body was refolded in urea gradient refolding buffer And then, the refolded protein was incubated with enterokinase to remove the His-tag The fusion protein without...
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Stability and in vivo evaluation of pullulan acetate as a drug nanocarrier
Ngày tải lên :
23/04/2013, 21:38
... stability and toxicity of PANs, and further investigated the sustained release behavior in vivo of drug loading in PANs PA was firstly synthesized and characterized by FT-IR and 1H NMR, and then ... sectioned, and stained with hematoxylin and eosin (H&E) In vivo pharmacokinetics and bioavailability In vivo pharmacokinetic study was conducted by the routine method (Mross et al., 1988; Bibby ... novel hydrophobized polysaccharide/oncoprotein complex vaccine induces in vitro and in vivo cellular and humoral immune responses against HER2-expressing murine sarcomas Cancer Res 58:3385–90 Gustafson,...
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Tài liệu Báo cáo khoa học: Pronounced adipogenesis and increased insulin sensitivity caused by overproduction of prostaglandin D2 in vivo pptx
Ngày tải lên :
16/02/2014, 09:20
... prostaglandin D2 in adipogenesis in vivo Y Fujitani et al indicate that overexpression of H-PGDS increases insulin sensitivity in vivo Adipocyte differentiation ex vivo Finally, we examined whether ... glucose, leptin and insulin, and insulin sensitivity, in TG mice After weeks of normal or HF diet, serum levels of triglyceride, glucose, leptin and insulin were deter- 1 Duration (week) 0 mined (Fig ... adipogenesis, and increased insulin sensitivity when on the HF diet Thus, we show, for the first time, that PGD2 is involved in the activation of adipogenesis and regulation of insulin sensitivity in vivo...
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Tài liệu Báo cáo khoa học: The endogenous retinoid metabolite S-4-oxo-9-cis-13,14-dihydro-retinoic acid activates retinoic acid receptor signalling both in vitro and in vivo pdf
Ngày tải lên :
18/02/2014, 08:20
... all-trans-retinoic acid, the findings clearly demonstrate that S-4o9cDH-RA has the capacity to bind and activate nuclear retinoid receptors and regulate gene transcription both in vitro and in vivo Retinoids ... [10]) There are different RAR and RXR subtypes (a, b and c), and each subtype exists in multiple isoforms [11] at-RA binds to the ligand-binding domain of RARs, which induces heterodimer formation ... experiment The subsequent incubation with the indicated retinoids was then terminated at the indicated time points by washing with NaCl ⁄ Pi and lysis of the cells using mL of Trizol (Invitrogen) per well...
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Tài liệu Báo cáo khoa học: Compartmentalization and in vivo insulin-induced translocation of the insulin-signaling inhibitor Grb14 in rat liver pptx
Ngày tải lên :
18/02/2014, 18:20
... Desbuquois et al Insulin-induced translocation of Grb14 in rat liver A H N B M LP S PM GE –ins Grb14 – ins Grb14 + ins –ins + ins + ins – ins EEA1 + ins Na+K+ATPase Calnexin + ins number: 10 11 ... [13], and tankyrase [15] The binding of ZIP to Grb14, by recruiting protein kinase Cf, enhances the serine phosphorylation of Grb14 and its inhibitory effect on IR kinase and insulin signaling ... liver insulin signaling, and that it may represent an interesting molecule with which to regulate liver insulin action Experimental procedures Materials Porcine insulin, recombinant human insulin,...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
Ngày tải lên :
22/02/2014, 07:20
... UP12 is a stress protein DISCUSSION In this work, we have identified two proteins GatY and UP12 as putative in vivo substrates of the chaperonin GroEL In addition to its in vivo interaction with GroEL ... members of this family including UspA itself (Fig 2), and one larger protein consisting of two UspA domains in tandem [17] The small members of this family are proteins of 142–144 amino-acids long; ... acrylamide in the separating and stacking gels, respectively In order to estimate the ratio between the amounts of UP12 and the total amount of protein in the extracts, a series of samples containing...
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Báo cáo khoa học: Efficient and targeted delivery of siRNA in vivo pdf
Ngày tải lên :
06/03/2014, 01:23
... required [12] In this minireview, major factors in determining overall RNAi efficiency in vivo are introduced Moreover, up-to-date progress in achieving efficient and targeted siRNA delivery in vivo, particularly ... successfully inhibited the expression of VEGF, thereby suppressing the growth of hepatocarcinoma tumors in mice [87] In addition, no noticeable increase in inflammatory cytokines, including interferon-a and ... obtained by in vitro transcription reaction and, therefore, avoid contamination by cell or bacterial products Promising in vitro and in vivo RNAi was obtained using siRNA that was directly linked...
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Báo cáo khoa học: Selectins and glycosyltransferases in leukocyte rolling in vivo pdf
Ngày tải lên :
07/03/2014, 12:20
... carrying the sialyl Lewis X (sLex) determinant During in ammation, the main in ammatory selectin ligand, P-selectin glycoprotein ligand-1 (PSGL-1), interacts with P- and L-selectin under in vivo ... rolling to firm leukocyte arrest [14] During in ammation, E- and P-selectin bind to selectin ligands expressed on rolling leukocytes (Table 1) In vivo studies using mice deficient in P-selectin ... nodes in core GlcNAcT-I– ⁄ – P- and E-selectin ligand-dependent rolling dramatically reduced in FucT-VII– ⁄ – during in ammation in vivo L-selectin-dependent rolling almost completely absent in...
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Báo cáo khoa học: Two types of replication protein A in seed plants Characterization of their functions in vitro and in vivo ppt
Ngày tải lên :
07/03/2014, 21:20
... USA) and transformed into E coli for protein induction Extracts prepared from cells induced for h were shown to contain a six-histidine C-terminal-tagged OsRPA14 fusion protein The OsRPA14 protein ... soluble in the presence of m urea Soluble fractions were loaded on to columns containing 10 mL His-Bind resin (Novagen) The columns were washed with 50 mL binding buffer containing m urea and then ... CM (1998) Interaction of human rad51 recombination protein with single-stranded DNA binding protein, RPA Nucleic Acids Res 26, 5388–5393 17 Stauffer ME & Chazin WJ (2004) Physical interaction...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
Ngày tải lên :
07/03/2014, 21:20
... shown) In conclusion, we have shown using both in vitro and in vivo methods, that PSI-G adopts a topology in the thylakoid membrane with the loop facing the stroma and its N- and C-termini facing ... after 0, 30 and 60 digestion with trypsin, probed with antibodies directed against PSI-G, PSI-K (intrinsic membrane protein, stromal loop), PSI-O (intrinsic membrane protein, luminal loop) and PSI-D ... promoter and terminator, and into vectors for in vitro transcription and translation An Arabidopsis line (psag-1.4 [5], termed DG in this report), lacking PSI-G due to a transposon footprint in exon...
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