in vivo models of lung disease

... as high as in the other two studies Our data are in line previous findings describing an impairment of lung function in subjects that are extremely overweight [12,20,21] Cigarette smoking is the ... studies in terms of the other determinants In KORA environmental tobacco smoke (ETS) and the use of at least one drug influencing lung function was associated with lower FEV1, while in the SHIP ... current analysis of three studies performed in South, Central and North Germany confirmed the integrity of the basic determinants of lung function that are usually taken into account in prediction...

Ngày tải lên: 12/08/2014, 11:21

... domain of Pats1 interacted with myosin heavy chain, whereas the deletion of Pats1 caused an alteration in the localization of myosin heavy chain [17] Moreover, over-expression of the kinase domain ... of in vivo studies in many nonmammal models have already provided timely information about molecular mechanisms of many disease processes and will continue to impact our understanding of disease ... moesin, a protein that anchors the actin cytoskeleton to the plasma membrane [24] Although cytoskeleton proteins are known as frequent ‘contaminants’ in the process of searching binding proteins,...

Ngày tải lên: 23/03/2014, 04:20

... variations of gait cycle timing in Parkinson's disease and Huntington's disease Mov Disord 1998, 13:428-437 Blin O, Ferrandez AM, Serratrice G: Quantitative analysis of gait in Parkinson patients: increased ... eventual failure of hind limb stepping Our findings of different effects of 3NP on gait dynamics of forelimbs and hind limbs are in accordance with previous motor behavioral assessments in 3NP-treated ... that braced their hind limbs against the inside walls of the walking compartment while simultaneously maintaining coordinated gait of the forelimbs [59] may suggest that 3NP-induced cognitive...

Ngày tải lên: 19/06/2014, 10:20

... regulated by retinoic acid and is often induced upon activation of this pathway [33] We were interested in confirming these findings and determining the kinetics of Crabp2 induction during OA Further, ... to joint instability or in OA Manipulation of the retinoic acid signalling pathway may prove useful in modifying the clinical course of OA The joint instability models presented herein in contrast ... retinoid signalling pathway were found to be significantly elevated in the joints of the aforementioned rat OA joint instability model, including genes encoding proteins involved in retinoic acid...

Ngày tải lên: 09/08/2014, 01:22

... possible An alternative mode of imaging is the use of single photon imaging of γ-emitting isotopes like 11 1In or 99Tc Imaging of γ-emitting isotopes is referred to as single photon emission commuted ... be collected in a couple of minutes of recording, the migration of leukocytes in tissues is relatively slow and requires many minutes of recording to track cells This longer imaging period requires ... signs of inflammation The groundwork for further studies on the dynamics of lymphocyte interactions in the synovium has thus been established 168 Most of the work in intravital imaging of leukocytes...

Ngày tải lên: 09/08/2014, 01:22

... of the study, in the interpretation of the data and in the revision of manuscript AA participated in the design and coordination of the study, in the interpretation of the data and in the revision ... found in sera of patients with various rheumatic diseases and interstitial pneumonia, including seven patients with SSc [23] Fibrosis leading to restrictive interstitial lung disease and increased ... fulfilled the inclusion criteria of: a definitive diagnosis of SSc according to the American College of Rheumatology [16]; a disease duration less than years from the onset of skin involvement;...

Ngày tải lên: 09/08/2014, 10:20

... microfibres to the tip of the miniprobe of mm in diameter, which can be inserted into the working channel of a fibreoptic bronchoscope [14] High-quality and real-time in vivo morphological images ... representing a non-invasive method that can be used to image the microscopic structure of airway wall tissue in vivo during a bronchoscopic procedure [13] The principle of this imaging method ... through the working channel of the bronchoscope and placed on the main carina of the airways Special care was taken to position the miniprobe perpendicularly to the surface of the main carina as much...

Ngày tải lên: 12/08/2014, 13:22

... Murine LN showing recruited cells (red fluorescent staining, see arrow) (g) Merger of (e) and (f ) showing a lack of nonspecific cellular staining (h) DAPI staining showing cell nuclei (original ... inflammation during an acute response that may offer insight into early proinflammatory cytokine release in the initial phases of the inflammatory response This is often lost by using models such ... critical in acute inflammation of murine joints in as early as 24 hours after zymosan injection (n = number of joints analyzed) in EL4/6.1 thymoma cells [31,64] From our previous findings, we...

Ngày tải lên: 12/08/2014, 14:22

... However, the effectiveness of regular use of inhaled glucocorticoids in the management of patients with CF remains uncertain based on the results of the various trials in which inhaled glucocorticoids ... polymorphisms in candidate genes involved in the inflammatory cascade have been shown to modulate the expression of the clinical phenotype [18,19] In several inflammatory diseases, variations in glucocorticoid ... sensitivity in a tissue-specific manner As the inflammatory process in CF is dominated by a neutrophil influx in the airways, our present findings reporting that CF patients carrying the BclI...

Ngày tải lên: 12/08/2014, 15:21

... were fixed in 10% phosphate buffered formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E) In vivo pharmacokinetics and bioavailability In vivo pharmacokinetic study ... after i.v at a dose of 200 mg/kg In vivo pharmacokinetics The plasma levels of EPI were determined following a single i.v injection of EPI or PA/EPI (10 mg/kg EPI equiv.) in female Wistar rats ... drug concentration of EPI and PA/EPI after i.v injection in rats at a single equivalent dose of 10 mg/kg Table 5.  Pharmacokinetic parameters of EPI and PA/EPI i.v injected in rats at the equivalent...

Ngày tải lên: 23/04/2013, 21:38

... satisfactory in the evaluation of nodal status in this patient group Since the highly cellular tissue in reactive lymph nodes may also show increased intensity, the role of DWI and ADC in distinguishing ... of ordinary leiomyomas (0.88) and cellular leiomyomas (1.19) (Figs 4, 5) Because ordinary leiomyomas tend to contain hyalinized collagen, the signal intensity of ordinary leiomyomas is hypotintensity ... images DWI can be explained with “T2 blackout effect”, which indicates hypointensity on DWI caused by hypointensity on T2-WIs, resulting in a decrease in the ADC of ordinary leiomyomas [28] ADC...

Ngày tải lên: 13/02/2014, 06:20

... are in agreement with those of a recent work in which GatY has been identified by other means as an in vivo substrate of GroEL [27] Interestingly, GatY is also included in a list of proteins that ... Services Department of the Weizmann Institute by a single injection of 150 lg of the purified protein, followed by two booster shots of the same amount of protein at 2-week intervals Serum was ... protein family [15] E coli contains five small members of this family including UspA itself (Fig 2), and one larger protein consisting of two UspA domains in tandem [17] The small members of this...

Ngày tải lên: 22/02/2014, 07:20

... spectrum of the porphyrin contained in Zn-PP-KatA, in a pyridine ⁄ water ⁄ NaOH ⁄ Tween-80 mixture, was not similar to that of the reference solution of Zn-PP in the same solvent, indicating that ... protein, in binding porphyrins It is not known how haem is transported intracellularly in bacteria, and the mechanisms by which haem is incorporated into soluble and membrane-bound proteins inside ... compounds being taken up into the cell and incorporated into vital haem proteins instead of haem [5] We show here that haem analogues are indeed taken up and can be incorporated into haem proteins,...

Ngày tải lên: 06/03/2014, 11:20

... sequences of the GAL genes through its N-terminal DNA binding site Gal80p inhibits the transcriptional activity of Gal4p by binding to its 28 amino acid region at the carboxyl terminal [25–27] In vitro ... prevalent in vivo Experimental procedures We consider four candidate models, Models I–IV, shown in Figs 1–3, to validate the mechanism of induction of GAL genes by galactose In each of the models, ... reflected by the sequence of interactions; (c) parameters quantifying strength of interactions; and (d) spatial localization of protein in a compartment or shuttling of proteins between compartments...

Ngày tải lên: 07/03/2014, 16:20

... autoradiograms (Figs and 3) In addition, an increased number of eosinophils were observed in lung interstitium of sensitized mice, confirming the eosinophilic response following Der p challenge (Fig ... selenocysteine residue in a C-terminal Sel-tag [18] Der p carrying the Sel-tag had an intact core sequence and maintained allergen-specific IgE-binding epitopes and the use of a Sel-tag enabled labelling ... deposited in the airways of mice and metabolized The labelling method involves the incorporation of a selenocysteine residue and the gamma-emitter selenium-75 (75Se) within an engineered C-terminal...

Ngày tải lên: 07/03/2014, 21:20

... shown in Fig 4C Interestingly, both tags are recognized more efficiently in the context of the PSI-G loop region than at the C-terminus Topology of PSI-G in vivo In order to examine the topology of ... disrupting protein–protein interactions within PSI, or by altering the conformation of the loop His-tags were employed in a topological study of the major light harvesting chlorophyll a ⁄ b binding ... or light harvesting complex (LHCI), consisting of four members (Lhca1–4) of the chlorophyll a ⁄ b binding protein family LHCI is bound along one side of PSI only, in the vicinity of the PSI-F and...

Ngày tải lên: 07/03/2014, 21:20

... reproduced in lung, which can explain in part the conflicting results found in adults and children given b-carotene or vitamin A [29] Moreover, in lung of rats injected with high-dose of vitamin A over ... and lung Total protein extracts were obtained as described in experimental procedures A single band of about 53 kDa was detected in liver and lung indicating that the amount of p53 protein was ... significantly increased in the liver and lung from vitamin A-deficient rats that in controls c-Jun protein was significantly decreased in vitamin A-deficient rats In the liver of rats with hypervitaminosis...

Ngày tải lên: 08/03/2014, 08:20

... observed, and this was independent of the kind of genetic lesion or inhibitor used, suggesting that this activating effect was indeed caused by the fatty acid/lipid environment of the ATPase It must ... and inhibitors acting on the same biosynthetic points has proved to be a useful way of distinguishing the in uence of direct effects, i.e sterol alteration, and indirect effects of EBI compounds ... cerevisiae, grown in the presence of octanoic acid [37] In this case, only Vmax was affected showing an increase that was accompanied by an increase in the presence of oleic acid in its plasma membrane...

Ngày tải lên: 08/03/2014, 22:20

... understanding of the production and function of miRNA in vivo as well as for monitoring the therapeutic potential of miRNAs in cancer Bioimaging of microRNA biogenesis The molecular mechanisms involved ... et al [22] obtained quantitative in vitro images of nine transcripts that were predicted to be miRNA9 targets using the ensemble machinelearning algorithm However, in vivo imaging of miRNA regulation ... the in vitro and in vivo investigations of miRNA production patterns Second, noninvasive monitoring of miRNAs provides useful information of the possible role of miRNAs involving cellular developments...

Ngày tải lên: 16/03/2014, 01:20

... previously to induce an increase in the level of [Ca2+]i and a correlated calpain activation [28] This increase in [Ca2+]i is more intense in aorta than in brain Under these conditions, in brain, no ... M Averna et al In vivo degradation of NOS and HSP90 by calpain B A C D Fig In vivo digestion of NOS and HSP90 in NMS rats during HSD treatment Aliquots (20 lg protein) of rat brain soluble material ... higher than in aorta, resulting in a much higher HSP90 to NOS ratio in brain (Fig 1C) A Calpain activation in rat brain and aorta following HSD treatment To promote in vivo calpain activation,...

Ngày tải lên: 30/03/2014, 04:20