in vivo assessment of metabolic compartmentation between neurons and astrocytes

... number of dose-contributing catheters increases Therefore, the catheter contribution index increases with the number of contributing catheters In case of a single contributing catheter, IP = In case ... contribution index (Eq 2) shown in color coding In case of two equally contributing catheters, Di/Dtot = 0.5 and IP = 2.0 IP is always in the range between and indicates that the catheter contribution index ... 5.4 mm and of interstitial edema (T2-w images) -5 ± 6.0 mm in plane, indicating a shift of the irradiation effect volume against the corresponding tolerance dose volume in the direction of the...

Ngày tải lên: 09/08/2014, 09:21

... stringent concentration of KCl, there was a correlation between inhibition of endonuclease activity and inhibition of splicing activity indicating that RNA binding can be monitored by measuring ... available and its binding affinity is not altered by the presence of DNA in the DNA binding site Interestingly, when nM RNA and 10 nM DNA were added simultaneously to a limiting amount of protein in ... determine the relative stoichiometry of RNA and DNA binding, nitrocellulose filter binding assays were performed (Fig 4A) Both DNA and RNA substrates saturated limiting amounts of protein in the...

Ngày tải lên: 17/03/2014, 10:20

... al In vivo degradation of NOS and HSP90 by calpain B A C D Fig In vivo digestion of NOS and HSP90 in NMS rats during HSD treatment Aliquots (20 lg protein) of rat brain soluble material (A) and ... brain and aorta The level of HSP90 and the type of NOS isoform present in rat brain and aorta were determined by immunoblotting (Fig 1) In brain, nNOS was the most preferentially expressed isoform, ... previously to induce an increase in the level of [Ca2+]i and a correlated calpain activation [28] This increase in [Ca2+]i is more intense in aorta than in brain Under these conditions, in brain, no...

Ngày tải lên: 30/03/2014, 04:20

... (anti-microtubular) agents include the naturally occurring vinca alkaloids (e.g vincristine and vinblastine) and their semi-synthetic analogues (e.g vinorelbine) and the taxanes (e.g paclitaxel and docetaxol) ... THE DEGREE OF MASTER OF ENGINEERING DEPARTMENT OF CHEMICAL AND BIOMOLECULAR ENGINEERING NATIONAL UNIVERSITY OF SINGAPORE 2011 Acknowledgements First of all, I would like to express my profound gratitude ... protein binding after IV administration (Extra et al., 1993) Docetaxel is mainly bound to alpha acid glycoprotein, lipoproteins, and albumin Among them, alpha acid glycoprotein is the main determinant...

Ngày tải lên: 09/10/2015, 11:18

... added into microwells and incubated at room temperature for 30 min, followed by three PBS washes Blocking for non-specific binding was performed by adding 100 lL of 3% bovine serum albumin (BSA) and ... that GNPs are capable of inducing an antibody response in mice, which indicates that factors other than cytotoxicity may be involved and complicates in vivo application of GNPs [16] The current ... albumin (BSA) and incubating for 60 at room temperature, followed by three PBS washes Binding was performed by adding 100 lL of diluted antiserum into microwells and incubating for h at room...

Ngày tải lên: 22/06/2014, 00:20

... reaction and the O2 electrode Fourth, to 10 μl of standard medium containing from to 400 nmols of ADP were added into the cell and the system again incubated until the O2 and ATP (contaminating the ... adding 400 nmols of ADP and 6.3 nmols of ATP (as contaminant of ADP) to an air-saturated medium free from RLM and succinate After 1.5 of incubation, 1.0 mg of RLM protein was added to initiate the ... all, ΔEh, O2 and ADP Thus, in the presence of NADH and 100 μM ADP the initial rates of ATP synthesis increase from near zero to 214 μmol · min-1 · mg prtoein-1 when the level of O2 increases from...

Ngày tải lên: 08/08/2014, 17:20

... upregulation of fatty acid oxidation in brown adipose tissue following the over expression of PPARδ in terms of the expression of a range of enzymes involved in uncoupling (uncoupling protein and 3), ... of PPARg and PPARδ in regulating metabolism in white adipose tissue, we have Page of 19 performed a metabolomics study using both in vivo analysis in the ob/ob mouse and in vitro analysis using ... detected using in vivo H NMR spectroscopy [17] In addition, an increase in the concentrations of creatine and phosphocreatine were detected in the gastrocnemius of ob/ob mice following pharmacological...

Ngày tải lên: 09/08/2014, 23:20

... since 1950 Searches were undertaken in MEDLINE and EMBASE, plus screening of reference lists in identified articles Potentially relevant reports, bulletins and guidelines were also screened, including ... younger ages and shows a decline as age increases, although this finding is not replicated in all studies, some show no association between the RR of TB amongst individuals with DM and of older age[12] ... found between diabetes and tuberculosis[28] In order to gain a better awareness of the effect of ethnicity upon the strength of the RR of individuals with DM for contracting TB further investigation...

Ngày tải lên: 11/08/2014, 14:21

... molecules candidate mediators of T-cell inhibition The involvement of iNOS and COX-2 in inhibition of T-cell proliferation was demonstrated by the addition of inhibitors of these enzymes - GW274150 and ... 3a) and because these cytokines have been shown to synergistically induce the expression of iNOS [34] and IDO [35] in fibroblasts The expression of iNOS, IDO, PD-L1, and COX-2, molecules involved ... subcutaneously injected with 20 μg of CII/20 μl of PBS in the right ear and with 20 μl of PBS in the left ear DTH response was calculated as the percentage swelling (the difference between the increase of...

Ngày tải lên: 12/08/2014, 11:23

... lung, and kidney were fixed in 10% phosphate buffered formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E) In vivo pharmacokinetics and bioavailability In vivo ... stability and toxicity of PANs, and further investigated the sustained release behavior in vivo of drug loading in PANs PA was firstly synthesized and characterized by FT-IR and 1H NMR, and then ... after i.v at a dose of 200 mg/kg In vivo pharmacokinetics The plasma levels of EPI were determined following a single i.v injection of EPI or PA/EPI (10 mg/kg EPI equiv.) in female Wistar rats...

Ngày tải lên: 23/04/2013, 21:38

... members of this family including UspA itself (Fig 2), and one larger protein consisting of two UspA domains in tandem [17] The small members of this family are proteins of 142–144 amino-acids ... Department of the Weizmann Institute by a single injection of 150 lg of the purified protein, followed by two booster shots of the same amount of protein at 2-week intervals Serum was collected and used ... growth and expression of UP12 Absorption (upper panel) and relative amount of UP12 (lower panel) in samples of cells grown in minimal M9 medium containing limited concentration of glucose (m) or in...

Ngày tải lên: 22/02/2014, 07:20

... the creation of an educator network between professional of both facilities and promoting the integration of inmates in the role of educators and counselors within health programs CORRESPONDENCE ... survey but, because of the small number of diabetic patients that we can find within a facility (39 in our case) and the difficulty of repeating coordinated activities of this type in the future, ... to working with the rest of the group Amongst our future objectives we would like to repeat the survey in three months, extending our experience in time and even intervening in the design of diabetic...

Ngày tải lên: 05/03/2014, 22:21

... that this is not due to inactivation during isolation of the protein Light absorption spectra of the purified iron-containing and gallium-containing catalases are presented in Fig 3A,B Fe-PP-KatA ... peak at 422 nm and distinct absorption maxima at 548 and 588 nm Screening for in vivo production of cofactor-substituted catalase The results obtained with Ga-PP and the properties of Ga-PP-KatA ... mechanisms of haemcontaining enzymes and electron transfer in proteins are greatly aided if the metal of the normal prosthetic group can be substituted The photosensitivity of, for example, zinc, tin and...

Ngày tải lên: 06/03/2014, 11:20

... reflected by the sequence of interactions; (c) parameters quantifying strength of interactions; and (d) spatial localization of protein in a compartment or shuttling of proteins between compartments ... sequences of the GAL genes through its N-terminal DNA binding site Gal80p inhibits the transcriptional activity of Gal4p by binding to its 28 amino acid region at the carboxyl terminal [25–27] In vitro ... concentration of Gal3p in the wild-type thus reducing the protein expression as in Model III This confirms the evidence obtained through gel filtering and cross-linking that Gal3p is monomeric in solution...

Ngày tải lên: 07/03/2014, 16:20

... selenocysteine residue in a C-terminal Sel-tag [18] Der p carrying the Sel-tag had an intact core sequence and maintained allergen-specific IgE-binding epitopes and the use of a Sel-tag enabled labelling ... lymph node proteins revealed a radioactive band of  25 kDa in liver and bands of  30 and 56 kDa in thoracic lymph nodes (Fig 5C) No radioactive protein bands could be detected in spleen samples ... the in ammatory state of the lungs [24] and that these cells play an essential role both in the induction and maintenance of allergen-driven eosinophilic airway in ammation [28–30] Trafficking of...

Ngày tải lên: 07/03/2014, 21:20

... harvesting complex (LHCI), consisting of four members (Lhca1–4) of the chlorophyll a ⁄ b binding protein family LHCI is bound along one side of PSI only, in the vicinity of the PSI-F and PSI-J ... shown in Fig 4C Interestingly, both tags are recognized more efficiently in the context of the PSI-G loop region than at the C-terminus Topology of PSI-G in vivo In order to examine the topology of ... disrupting protein–protein interactions within PSI, or by altering the conformation of the loop His-tags were employed in a topological study of the major light harvesting chlorophyll a ⁄ b binding...

Ngày tải lên: 07/03/2014, 21:20

... [27] In rats Fig Western blot analysis of p53 and c-Jun in liver and lung Total protein extracts were obtained as described in experimental procedures A single band of about 53 kDa was detected in ... liver and lung indicating that the amount of p53 protein was significantly increased in the liver and lung from vitamin A-deficient rats that in controls c-Jun protein was significantly decreased in ... cared and handled in conformence with the Guiding Principles for Research Involving Animals and Humans, approved by the Council of the American Physiological Soceity The School of Medicine Research...

Ngày tải lên: 08/03/2014, 08:20

... Three Areas of Training Emphasis: Performance, Checklist, Feedback The Goal of Training Session Three The Training Setting Summary of the Training Activities Reminders Session Three Training Activities ... 12 Training Session Four: First Dress Rehearsal (Clinician Verification of SPs’ Authenticity) The Goal of Training Session Four The Training Setting Summary of the Training Activities Reminders ... standardized patients in medical education puts us in touch with such an ancient and noble history and gratifying to think that the arts of acting and medicine once again are being joined in the 21st century—this...

Ngày tải lên: 08/03/2014, 16:20

... mixture of oleic and linoleic acid-containing phosphatidylcholine was added (82.9 ± 8.9% of the control) Conversely, when 1-palmytoyl-2-linoleyl-phosphatidylcholine was added to these vesicles, an increase ... and inhibitors acting on the same biosynthetic points has proved to be a useful way of distinguishing the in uence of direct effects, i.e sterol alteration, and indirect effects of EBI compounds ... activity in lmol Pi min)1Æmg)1Æprotein j, No additions; d, + 50 lM vanadate; m, + 30 lM erythrosin B sterols, have on membrane properties As in past reports, the joint use of mutants and inhibitors...

Ngày tải lên: 08/03/2014, 22:20

... understanding of the production and function of miRNA in vivo as well as for monitoring the therapeutic potential of miRNAs in cancer Bioimaging of microRNA biogenesis The molecular mechanisms involved ... vector containing three tandem repeat regions of miRNA23 binding sequence (reprinted with permission [11]) information for understanding the miRNA biogenesis related to human diseases Imaging miRNA ... et al [22] obtained quantitative in vitro images of nine transcripts that were predicted to be miRNA9 targets using the ensemble machinelearning algorithm However, in vivo imaging of miRNA regulation...

Ngày tải lên: 16/03/2014, 01:20