in vivo antitumor activity of cryptophycin 52 ly355703

Tài liệu Báo cáo khoa học: The role of electrostatic interactions in the antitumor activity of dimeric RNases docx

Tài liệu Báo cáo khoa học: The role of electrostatic interactions in the antitumor activity of dimeric RNases docx

... Electrostatic interactions and antitumor RNases the dimer interface of the models or in the surroundings of the hinge loop residues Likewise, the models of nonexchanging and exchanging HHP-RNase ... detected at the dimer interface of the models or in the surroundings of the mutated residues The models of RNase A covalent dimers in the exchanging conformation were prepared using the 7RSA structure ... values of EIE were recorded at 30° intervals of r from to 360°; (d) the inclination of the dimer major axis with respect to the z axis, hence to the membrane plane, was changed (by inclination of...

Ngày tải lên: 19/02/2014, 06:20

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Báo cáo y học: "In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures" pps

Báo cáo y học: "In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures" pps

... structures of acetylshikonin (I) and shikonin (II) Chemical structures of acetylshikonin (I) and shikonin (II) cells Natural shikonin-like compounds also have significant in vivo antitumor effects In ... http://www.cmjournal.org/content/4/1/14 Inhibition rate % Chinese Medicine 2009, 4:14 acetylshikonin μg/ml Figure cells (n = effects of acetylshikonin on the growth of tumor Inhibitory 3) Inhibitory effects of acetylshikonin on ... was obtained from the Cell Bank of the Chinese Academy of Sciences The cells were cultured in RPMI 1640 (Gibco, USA) supplemented with 10% fetal bovine serum (Minhai Bio-engineering, China) MTT...

Ngày tải lên: 13/08/2014, 15:21

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báo cáo khoa học: " Antitumor activity of mixed heat shock protein/ peptide vaccine and cyclophosphamide plus interleukin-12 in mice sarcoma" pot

báo cáo khoa học: " Antitumor activity of mixed heat shock protein/ peptide vaccine and cyclophosphamide plus interleukin-12 in mice sarcoma" pot

... important single cytokine in inducing antitumor immunity In Page of experimental tumor models, recombinant IL-12 has demonstrated marked antitumor effects through mechanisms of both innate and ... Lungs, liver and brains of dead mice were removed and fixed in formalin, embedded in paraffin, Page of and sectioned at μm Hematoxylin & eosin (H&E) stained samples were examined under a light ... to #6 contained proteins of about 40-200 kDa The combination of these fractions was used as the mHSP/Ps vaccine The identity of proteins in this combination was assayed using SDS-PAGE and Western...

Ngày tải lên: 10/08/2014, 10:21

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Báo cáo khoa học: Investigating the role of the invariant carboxylate residues E552 and E1197 in the catalytic activity of Abcb1a (mouse Mdr3) docx

Báo cáo khoa học: Investigating the role of the invariant carboxylate residues E552 and E1197 in the catalytic activity of Abcb1a (mouse Mdr3) docx

... trypsin-sensitive region situated in the linker domain of Abcb1a Following trapping in the absence or presence of Vi and mild-trypsin treatment, the trypsin degradation products of the two mutants R ⁄ Q ... for trapping is almost normal By contrast, when the charge is removed, as in the glutamine (length of the side chain maintained) and asparagine (shorter side chain) mutants, then trapping in the ... dimerization of its nucleotide-binding domains Nature 433, 876–880 52 Vogan KJ & Gros P (1997) The C-terminal subdomain makes an important contribution to the DNA binding activity of the Pax-3...

Ngày tải lên: 07/03/2014, 06:20

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Báo cáo khoa học: Contribution of exofacial thiol groups in the reducing activity of Lactococcus lactis docx

Báo cáo khoa học: Contribution of exofacial thiol groups in the reducing activity of Lactococcus lactis docx

... identication of proteins located on the extracellular surface and involved in the decrease in Eh would be of interest for increasing our understanding of the mechanisms involved as well as the reducing activity ... attractive model for investigating new mechanisms involved in the reducing activity of microorganisms This was the aim of the present study Thiol groups in the reducing activity of L lactis 50 5.0 ... reducing metabolisms L lactis is able to maintain a low Eh7 until the end of fermentation and for 24 h [16] The role of the active mechanisms in maintaining a low reducing Eh7 was thus investigated...

Ngày tải lên: 22/03/2014, 21:21

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Báo cáo khoa học: Analysis of the in planta antiviral activity of elderberry ribosome-inactivating proteins potx

Báo cáo khoa học: Analysis of the in planta antiviral activity of elderberry ribosome-inactivating proteins potx

... type-2 RIPs but possesses a B chain which, because of several amino-acid substitutions in the sugarbinding sites, is devoid of carbohydrate-binding activity [11] Initially, RIPs were thought to ... ribosomes [39], and accordingly is capable of depurinating in planta part of the ribosomes in IRIPexpressing tobacco on TMV infection (as could be demonstrated by an in planta depurination assay) [40] ... Purification of recombinant proteins rRIPs were isolated using a combination of classical protein purification techniques and affinity chromatography (except for SNLRP, which possesses no sugar-binding activity) ...

Ngày tải lên: 23/03/2014, 12:20

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Báo cáo khoa học: In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I pot

Báo cáo khoa học: In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I pot

... pneumoniae SPase I For in vitro self-cleavage of S pneumoniae SPase I in the presence of phospholipid, 20 lL of reaction containing lg of purified SPase I was incubated in 37 °C for h in 20 mM Tris/HCl ... explain why self-cleavage is happening in vivo, one speculation is that it may be involved in regulating the activity of the enzyme It is not difficult to imagine that bacteria may secrete proteins ... cleavage of prestreptokinase, typically, reactions (20 lL) containing 0.1 lg SPase I or SPase37–204 were incubated with lg of purified prestreptokinase at 37 °C for h in the buffer containing 20...

Ngày tải lên: 23/03/2014, 21:21

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Báo cáo khoa học: In vivo cross-linking of nucleosomal histones catalyzed by nuclear transglutaminase in starfish sperm and its induction by egg jelly triggering the acrosome reaction pdf

Báo cáo khoa học: In vivo cross-linking of nucleosomal histones catalyzed by nuclear transglutaminase in starfish sperm and its induction by egg jelly triggering the acrosome reaction pdf

... demonstration of intracellular transglutaminase-catalyzed protein cross-linking in vivo Experimental procedures Collection of sperm Specimens of A pectinifera were collected, during their breeding season, ... Ó FEBS 2003 In vivo cross-linking of histones (Eur J Biochem 270) 3755 Fig In situ monodansylcadaverine (DCA) labeling of histones in sperm nuclei A sperm nuclear suspension was incubated for ... transglutaminase Occurrence of transglutaminase in sperm chromatin We next examined whether nuclear transglutaminase is present in the chromatin fraction by carrying out an immunoblot analysis using...

Ngày tải lên: 31/03/2014, 07:20

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Báo cáo sinh học: " In vivo dose-response of insects to Hz-2V infection" pptx

Báo cáo sinh học: " In vivo dose-response of insects to Hz-2V infection" pptx

... that virus dose affects both the level of infected progeny and the kind of infection found in insects hatching from eggs laid by virus infected females, indicating a direct correlation between virus ... the large increase in virus over a 24 hour cycle observed in vitro also occurs in vivo, resulting in a significant daily increase in the titer of Hz-2V in these experimentally infected female moths ... Hz-2V replication in vitro revealed a rapid increase in virus titer by 24 hours post infection in Tn-368 and Ld-652Y cells [4,5] Hz-2V replication in vivo in the epithelial cells of agonadal female...

Ngày tải lên: 18/06/2014, 22:20

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báo cáo hóa học:" In vivo dose-response of insects to Hz-2V infection" pdf

báo cáo hóa học:" In vivo dose-response of insects to Hz-2V infection" pdf

... that virus dose affects both the level of infected progeny and the kind of infection found in insects hatching from eggs laid by virus infected females, indicating a direct correlation between virus ... the large increase in virus over a 24 hour cycle observed in vitro also occurs in vivo, resulting in a significant daily increase in the titer of Hz-2V in these experimentally infected female moths ... Hz-2V replication in vitro revealed a rapid increase in virus titer by 24 hours post infection in Tn-368 and Ld-652Y cells [4,5] Hz-2V replication in vivo in the epithelial cells of agonadal female...

Ngày tải lên: 20/06/2014, 04:20

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Báo cáo toán học: " Antitumor activity of sorafenib-incorporated nanoparticles of dextran/poly(DL-lactide-co-glycolide) block copolymer" pdf

Báo cáo toán học: " Antitumor activity of sorafenib-incorporated nanoparticles of dextran/poly(DL-lactide-co-glycolide) block copolymer" pdf

... higher the initial drug feeding, the higher is the quality of incorporated sorafenib The size of the nanoparticles was increased according to the amount of sorafenib Increasing quantity of incorporated ... Republic of Korea Department of Internal Medicine, Medical Research Institute, Pusan National University School of Medicine and Medical Research Institute, Yangsan, 626-770, Republic of Korea ... following equations: Drug content = Drug weight in the nanoparticles × 100 Weight of the nanoparticles and Loading efficiency = Residual drug in the nanoparticles × 100 Initial feeding amount of...

Ngày tải lên: 20/06/2014, 20:20

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báo cáo hóa học:" Antitumor activity of sorafenib-incorporated nanoparticles of dextran/poly(DL-lactide-co-glycolide) block copolymer" pptx

báo cáo hóa học:" Antitumor activity of sorafenib-incorporated nanoparticles of dextran/poly(DL-lactide-co-glycolide) block copolymer" pptx

... higher the initial drug feeding, the higher is the quality of incorporated sorafenib The size of the nanoparticles was increased according to the amount of sorafenib Increasing quantity of incorporated ... Republic of Korea Department of Internal Medicine, Medical Research Institute, Pusan National University School of Medicine and Medical Research Institute, Yangsan, 626-770, Republic of Korea ... following equations: Drug content = Drug weight in the nanoparticles × 100 Weight of the nanoparticles and Loading efficiency = Residual drug in the nanoparticles × 100 Initial feeding amount of...

Ngày tải lên: 21/06/2014, 17:20

14 320 0
Báo cáo vật lý: "Evaluation of In Vitro Antioxidant Activity of 5H-dibenz[b,f]azepine and Its Analogues" ppt

Báo cáo vật lý: "Evaluation of In Vitro Antioxidant Activity of 5H-dibenz[b,f]azepine and Its Analogues" ppt

... performed in the absence of the compound to determine the amount of lipid peroxidation obtained in the presence of inducing agents as a control experiment The percentage of antilipid peroxidative activity ... preparation of 10-methoxy-5H-dibenz[b,f]azepine (Compound d) 10-methoxy-5H-dibenz[b,f]azepine was prepared by brominating N-acetyl-5H-dibenz[b,f]azepine (2.35 g, 10 mM) using bromine (3.2 g, 20 mM) in ... outlined in schemes 1–3 In scheme 3, to obtain compound (e), the reaction was carried out by using a weak base (triethyl amine) with COCl2 at room temperature (RT) instead of triphosgene in the...

Ngày tải lên: 07/08/2014, 14:20

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Báo cáo y học: "In vivo bactericidal activities of Japanese rice-fluid against H. pylori in a Mongolian gerbil model" ppsx

Báo cáo y học: "In vivo bactericidal activities of Japanese rice-fluid against H. pylori in a Mongolian gerbil model" ppsx

... all over Japan In this study, we investigated the antibiotic effect of JRF on H pylori infection in vivo and its anti-inflammatory effects on the H pylori-induced gastritis, using a Mongolian ... for the detection of mucin-containing cells Because the inflammatory component of the mucosa was virtually identical to that found in human H pylori gastritis, the degree of inflammation could ... Histopathological findings In animals from non- H pylori inoculated groups (C and D), inflammatory cell infiltration into the lamina propria was negligible (Table and Fig 2C) In contrast, active...

Ngày tải lên: 08/08/2014, 16:23

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In vitro and in vivo targeted delivery of IL-10 interfering RNA by JC virus-like particles pptx

In vitro and in vivo targeted delivery of IL-10 interfering RNA by JC virus-like particles pptx

... and the effects of cell apoptosis were determined by 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI) staining (C) Cell viability was determined by trypan blue staining VLPs IL-10 ... results in the production of multiple inflammatory cytokines, including IL-1, INF-γ and TNF-α production, which is known to cause tissue necrosis and organ failure In addition to inducing the ... production of pro-inflammatory cytokines, LPS also triggers the release of anti-inflammatory cytokines, such as IL-10, in the bloodstream of normal mice [17] Many cell types can produce IL-10, including...

Ngày tải lên: 10/08/2014, 05:21

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Báo cáo y học: " In-vitro antiviral activity of Solanum nigrum against Hepatitis C Virus" pdf

Báo cáo y học: " In-vitro antiviral activity of Solanum nigrum against Hepatitis C Virus" pdf

... of Dimethyl sulfoxide (DMSO) ensuring stock concentration of 50 μg/μl Sieving the above solution by using 0.22 um filter inside Laminar Flow Hood, storing at -20°C Cell line The Huh-7 cell line ... plasmids containing HCV NS3 protease in the presence and absence of SN 100 μg extract and interferon by using Lipofectamine™ 2000 (Invitrogen life technologies, Carlsbad, CA) according to the manufacturer’s ... there is no vaccine available for prevention of HCV infection due to high degree of strain variation The current treatment of care, Pegylated interferon a in combination with ribavirin is costly,...

Ngày tải lên: 11/08/2014, 21:21

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Báo cáo sinh học: "In vivo gene targeting of IL-3 into immature hematopoietic cells through CD117 receptor mediated antibody gene delivery" pot

Báo cáo sinh học: "In vivo gene targeting of IL-3 into immature hematopoietic cells through CD117 receptor mediated antibody gene delivery" pot

... affecting the bioavailabilty of the administered conjugates strongly determine their in vivo performance These include avid interaction with serum components, resulting in colloidal instability, including ... set of experiments, two intraperitoneal injections of chloroquine (32.5 mg/kg) were performed hours and just a few minutes before intravenous injection of conjugates The tolerance of chloroquine ... plasmid injection in blood, the presence of plasmid might be due to the intravenous administration route used and in kidney, to a progressive elimination of the plasmid in this organ of refinement...

Ngày tải lên: 14/08/2014, 19:22

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The quality of financial assessment in the lending activity of Viet Nam commercial banks (Vietnam Joint Stock Commercial Bank for Industry and Trade)

The quality of financial assessment in the lending activity of Viet Nam commercial banks (Vietnam Joint Stock Commercial Bank for Industry and Trade)

... evaluation of the investment project involving lending activity of the commercial banks 1.4.2 The indicators to the quality of the financial evaluation of the investment project involving lending activity ... awareness of the role of financial appraisal of investment projects Organization of expert training, professional financial evaluation of of investment projects 4.2.2 The solution of the information ... evaluation of the investment project involving lending activity of the commercial banks 1.4.1 The definition of the quality of the financial evaluation of the investment project - Check before lending...

Ngày tải lên: 02/05/2016, 23:12

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Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt

Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt

... conformational transition upon binding to BchH may bring the integrin I domain of BchD into contact with the integrin-binding motif of BchH, simultaneously triggering porphyrin metallation This would ... shown binding of Mg2+ to this subunit [21] An integrin I domain, suggested to exist in the C-terminus of the 70 kDa subunit, is a metal binding site (MIDAS motif) that can be expected to bind Mg2+ ... release of the blockade of the ATP-binding site of BchI by the integrin I domain, triggering ATP hydrolysis [10] It is still an open question which subunit provides the Mg2+ to be inserted into...

Ngày tải lên: 19/02/2014, 12:20

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Báo cáo Y học: In vivo activation of plasma membrane H+-ATPase hydrolytic activity by complex lipid-bound unsaturated fatty acids in Ustilago maydis docx

Báo cáo Y học: In vivo activation of plasma membrane H+-ATPase hydrolytic activity by complex lipid-bound unsaturated fatty acids in Ustilago maydis docx

... observed, and this was independent of the kind of genetic lesion or inhibitor used, suggesting that this activating effect was indeed caused by the fatty acid/lipid environment of the ATPase It must ... to oleic acid and H+-ATPase hydrolytic activity in plasma membrane vesicles of U maydis Specific activity in lmol PiÆmin)1Æmg)1 protein Line generated by linear regression (r ¼ 0.980) curve for ... (data not shown) Changes in activity were observed to be the result of an increase in Vmax with little changes in affinity for MgATP Changes in Vmax correlated with increases in À1 18 : 2/18 : ratio...

Ngày tải lên: 08/03/2014, 22:20

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