in vivo antitumor activity and toxicity

... acetylshikonin (I) and shikonin (II) Chemical structures of acetylshikonin (I) and shikonin (II) cells Natural shikonin-like compounds also have significant in vivo antitumor effects In a study ... SYUNZ-7, a shikonin derivative, showed antitumor effects both in vivo and in vitro Xie et al [6] showed that some shikonin derivatives were more powerful than natural shikonin in terms of antitumor ... acetylshikonin The IOD of each group indicates that the expression of bax and caspase-3 in the acetylshikonin groups increased, whereas bcl-2 decreased in the same groups, resulting in 0.8 Adriamycin...

Ngày tải lên: 13/08/2014, 15:21

... Electrostatic interactions and antitumor RNases the dimer interface of the models or in the surroundings of the hinge loop residues Likewise, the models of nonexchanging and exchanging HHP-RNase ... cytotoxicity Two RNases, homologous to the RNases investigated in this study, have been isolated from human eosinophils, namely eosinophil-derived neurotoxin (EDN) and eosinophil cationic protein ... and variation of the inclination (inclination angle ‘i’) with respect to the plane of the membrane (parallel to the xy plane) Cter and Nter are the C-terminal and N-terminal structural regions,...

Ngày tải lên: 19/02/2014, 06:20

... Fig In vitro transfection activity of cationic lipids in the absence of helper lipid(s) (A) and in the presence of DOPE (B) or DOPE and cholesterol (C), as measured in a murine skin cell line ... series by only a single amine in the polar headgroup, elicited transfection activity Addition of a second amine group and the subsequent increase in rM increased the Novel cytofectins for gene delivery ... and maintained in serum medium (Dulbeccos modied Eagles medium supplemented with 10% fetal bovine serum, 50 unitsặmL)1 penicillin and 50 lgặmL)1 streptomycin) After incubation for h at 37 C in...

Ngày tải lên: 23/03/2014, 07:20

... antioxidant, anti -in ammatory, antitumor, and antiviral properties [14] Rutin (quercetin-3-O-rutinoside), the glycoside of quercetin, is abundantly found and distributed in plants such as in buckwheat ... radical, and peroxyl radical [15], as shown by many in vitro and in vivo experiments [16–18] Furthermore, rutin has several pharmacological activities including anti-allergic, anti -in ammatory, and ... solution containing the rutin (120 ml) was added to this organic phase maintaining the agitation for a further 10 Then, the organic phase was injected into an aqueous phase (534 ml) containing Tween...

Ngày tải lên: 21/06/2014, 17:20

... buds and branches which formed within the whorl and on the interwhorl stem of each flush was counted on several occasions during summer High N plants were more vigorous than low N plants producing ... on interwhorl stems for the st and 2nd flushes of growth are shown in Table II The values were always

Ngày tải lên: 09/08/2014, 02:21

... as participated in all assays SL participated in B-LCL phenotyping and staging assay TS participated in B-LCL phenotyping, staging, and fusion assay TC participated in staging and fusion assay ... following in vivo infection with SIVmac251 tion by preventing early reverse transcription in vitro APOBEC-3G, a cellular cytidine deaminase, induces C to U mutations in the negative strand of ... SIV restricting molecule, our findings underscore that an innate antiviral response, which is capable of inhibiting early RT, can impact the in vivo clinical outcome of the animals infected with...

Ngày tải lên: 12/08/2014, 04:20

Ngày tải lên: 14/11/2014, 06:30

... with formalin and paraffin embedded by standard histological examination To assess gross tissue morphology, sections were stained with hematoxylin and eosin A vaginal irritation grading system ... inhibit the entry of HIV-1 into T cells and may limit the reverse transcription step into macrophages and DC These findings indicate that C14 harbours potent HIV-1 entry inhibition activity and/ ... particles in vitro [25], caused lesions in the vaginal epithelium in vivo and increased the probability of being infected with HIV-1 [26] To assess whether biological activity of C14 causes inflammation...

Ngày tải lên: 10/08/2014, 05:21

... misfolding of the short Erv1p because FAD binding and in vitro enzyme activity are unchanged Changing C30 or C33 to serine drastically increases the amount of observed dimers Probably, the remaining ... pairs of cysteines in Erv1p are indispensable for in vivo activity The six cysteines of yeast Erv1p can be grouped into three pairs (Fig 1): one pair near the N-terminus of the protein, the central ... are no clashes of amino acid side chains in the core of the structure, and most mutations occur on the protein surface The FAD binding groove is conserved with most ligand binding residues identical...

Ngày tải lên: 17/03/2014, 10:20

... collected in lithium heparincontaining tubes at the same time points and also two weeks after the sixth injection (day 85) for monitoring both the APC (monocytes, dendritic cells) and CD8 T cell ... compare the immunomonitoring and clinical values obtained at the different time points Only patients with all available time points were included in the immunomonitoring analysis Spearman rank ... immunotherapy combined with chemotherapy in a first-line setting with patients with a good immune status, as here, but in randomized phase II and III clinical trials with standard chemotherapy...

Ngày tải lên: 18/06/2014, 16:20

... leukemia cells) were obtained from KCLB (Korean Cell Line Bank, Seoul, Korea) and maintained in RPMI medium containing 10% (v/v) fetal bovine serum (FBS, GibcoBRL, Grand Island, NY) in a humidified atmosphere ... L casei and B longum showed high activity on all tumor cells, and led us in vivo antitumor study with these strains Our data showing different antiproliferative activity in these strains consist ... suggesting that both L casei and B longum in intestinal microflora can activate immune system to prevent diseases including tumors References Fig Survival rate of F9-bearing mice after LABs administration...

Ngày tải lên: 07/08/2014, 17:22

... months Lungs, liver and brains of dead mice were removed and fixed in formalin, embedded in paraffin, Page of and sectioned at μm Hematoxylin & eosin (H&E) stained samples were examined under a light ... important single cytokine in inducing antitumor immunity In Page of experimental tumor models, recombinant IL-12 has demonstrated marked antitumor effects through mechanisms of both innate and adaptive ... response induced by vaccines and synergized with vaccine in therapy but not in prophylaxis tumor models [44] With our enhanced vaccine, IFN-g secretion was significantly increased In addition, CD8+ and...

Ngày tải lên: 10/08/2014, 10:21

... characterized by insulin resistance and insulin secretion deficiency At present, there is no a single medication which treats type diabetes by improving both insulin resistance and insulin secretion ... fusion protein exhibited significant glucose-lowering effect in diabetic mice and may be a promising agent that can treat type daibetes by improving both insulin resistance and insulin secretion ... fusion protein in inclusion body was refolded in urea gradient refolding buffer And then, the refolded protein was incubated with enterokinase to remove the His-tag The fusion protein without...

Ngày tải lên: 25/10/2012, 11:10

... stability and toxicity of PANs, and further investigated the sustained release behavior in vivo of drug loading in PANs PA was firstly synthesized and characterized by FT-IR and 1H NMR, and then ... novel hydrophobized polysaccharide/oncoprotein complex vaccine induces in vitro and in vivo cellular and humoral immune responses against HER2-expressing murine sarcomas Cancer Res 58:3385–90 Gustafson, ... sectioned, and stained with hematoxylin and eosin (H&E) In vivo pharmacokinetics and bioavailability In vivo pharmacokinetic study was conducted by the routine method (Mross et  al., 1988; Bibby...

Ngày tải lên: 23/04/2013, 21:38

... 2001, 2003, and 2006 The SNHS is an ongoing, home-based personal interview examining a national representative sample of non-institutionalized population residing in main family dwellings (households) ... Spain and is mainly performed by the Ministry of Health and Consumer Affairs and the National Statistics Institute (Instituto Nacional Estadística-INE) The SNHS uses a multistage cluster sampling, ... proportional random selection of primary and secondary sampling units (towns and sections, respectively), with the final units (individuals) being selected by means of random routes and sex- and age-based...

Ngày tải lên: 14/02/2014, 06:20

... prostaglandin D2 in adipogenesis in vivo Y Fujitani et al indicate that overexpression of H-PGDS increases insulin sensitivity in vivo Adipocyte differentiation ex vivo Finally, we examined whether ... glucose, leptin and insulin, and insulin sensitivity, in TG mice After weeks of normal or HF diet, serum levels of triglyceride, glucose, leptin and insulin were deter- 1 Duration (week) 0 mined (Fig ... adipogenesis, and increased insulin sensitivity when on the HF diet Thus, we show, for the first time, that PGD2 is involved in the activation of adipogenesis and regulation of insulin sensitivity in vivo...

Ngày tải lên: 16/02/2014, 09:20

... antibody interaction in the presence of increasing concentrations of NaCl In both experiments, binding intensities are represented in RU, and each data point shows the mean value ± standard deviation ... immunoreactivity, and in B C E Fig Exposure of IgG to a pH buffer results in increased antigen-binding polyreactivity (A) Densitometric profiles of the reactivity of the native (solid line) and low-pH ... lgÆmL)1 (lines 1–3) and 50 lgÆmL)1 (lines 4–6) (C) Increased binding of pH bufferexposed IVIg to recombinant human IFN-c (D) Increased binding of low-pH buffer-exposed IVIg to human factor H In both...

Ngày tải lên: 16/02/2014, 15:20

... concentrations in Lowermoor treatment works and in trunk main system Indications of copper concentrations in the contaminated water Other water pollution incidents involving aluminium sulphate Key points ... to have complained later of continuing or new symptoms following the incident These symptoms have included joint and muscle pains, memory loss, hypersensitivity and gastrointestinal disorders ... prevalence of joint pains and/ or swelling in the population receiving contaminated water is higher than normal Monitoring of routine health statistics 1.36 The monitoring of routine health statistics...

Ngày tải lên: 17/02/2014, 10:20

... all-trans-retinoic acid, the findings clearly demonstrate that S-4o9cDH-RA has the capacity to bind and activate nuclear retinoid receptors and regulate gene transcription both in vitro and in vivo Retinoids ... [10]) There are different RAR and RXR subtypes (a, b and c), and each subtype exists in multiple isoforms [11] at-RA binds to the ligand-binding domain of RARs, which induces heterodimer formation ... experiment The subsequent incubation with the indicated retinoids was then terminated at the indicated time points by washing with NaCl ⁄ Pi and lysis of the cells using mL of Trizol (Invitrogen) per well...

Ngày tải lên: 18/02/2014, 08:20

... Desbuquois et al Insulin-induced translocation of Grb14 in rat liver A H N B M LP S PM GE –ins Grb14 – ins Grb14 + ins –ins + ins + ins – ins EEA1 + ins Na+K+ATPase Calnexin + ins number: 10 11 ... [13], and tankyrase [15] The binding of ZIP to Grb14, by recruiting protein kinase Cf, enhances the serine phosphorylation of Grb14 and its inhibitory effect on IR kinase and insulin signaling ... liver insulin signaling, and that it may represent an interesting molecule with which to regulate liver insulin action Experimental procedures Materials Porcine insulin, recombinant human insulin,...

Ngày tải lên: 18/02/2014, 18:20