antioxidant activity in vivo model
Báo cáo khoa học: LRRK2 in Parkinson’s disease: in vivo models and approaches for understanding pathogenic roles pdf
Ngày tải lên :
23/03/2014, 04:20
... ⁄ function-related pro-
teins (e.g. ROCO family proteins) in various model
systems, including lower eukaryotes and invertebrates,
in order to obtain clues for building important hypoth-
eses. ... moesin, a protein
that anchors the actin cytoskeleton to the plasma mem-
brane [24]. Although cytoskeleton proteins are known
as frequent ‘contaminants’ in the process of searching
binding proteins, ... in fruit flies have provided
important in vivo information regarding the potential
function of LRRK2 (Table 1). Indeed, certain observa-
tions reported in fly models may appear to be conflict-
ing....
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Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt
Ngày tải lên :
19/02/2014, 12:20
... double-
ringed BchI-BchD structure the ATPase activity of BchI is
blocked. A conformational transition upon binding to
BchH may bring the integrin I domain of BchD into contact
with the integrin-binding ... ion coordination motif
characteristic for integrin I domains [10]. Integrins are
known to participate in cell–matrix and cell–cell interactions
[14,15]. They are involved in signalling to and ... catalyzing cobalt
insertion in hydrogenobyrinic acid a,c-diamide during coenzyme
Ó FEBS 2004 ATPase activity is required to maintain subunit D (Eur. J. Biochem. 271) 2187
cDNAwasthenusedinanordinaryPCRamplification
and...
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Báo cáo khoa học: A steady-state modeling approach to validate an in vivo mechanism of the GAL regulatory network in Saccharomyces cerevisiae ppt
Ngày tải lên :
07/03/2014, 16:20
... and Bioengineering, Indian Institute of Technology Bombay,
Powai, Mumbai, India
Cellular regulation is a result of complex interactions arising
from DNA–protein and protein–protein binding, autoreg-
ulation, ... present in m ultiple c opies representing variable binding
sites. Furthermore, the status of one binding site could
in uence the binding of the regulatory protein to the other,
representing cooperativity ... genes
through i ts N -terminal DNA binding site. Gal80p inhibits
the transcriptional activity of Gal4p b y binding to its 2 8
amino acid region at the carboxyl terminal [25–27]. In vitro
studies have...
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Báo cáo khoa học: A mouse model for in vivo tracking of the major dust mite allergen Der p 2 after inhalation docx
Ngày tải lên :
07/03/2014, 21:20
... homeostasis in the air-
way mucosa is maintained in order to prevent respirat-
ory infections as well as allergic manifestations.
However, in an increasing proportion of the popula-
tion in industrialized ... challenge in the
airways. In this model, we applied recombinant Der p 2 carrying a novel
C-terminal tetrapeptide Sel-tag enabling labelling with the gamma-emitting
radionuclide
75
Se at a single ... con-
taining selenocysteine might not be presented by the
major histocompatibility complex (MHC) but rather
metabolized into selenocysteine-containing proteins.
This is consistent with our finding...
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Báo cáo Y học: In vivo activation of plasma membrane H+-ATPase hydrolytic activity by complex lipid-bound unsaturated fatty acids in Ustilago maydis docx
Ngày tải lên :
08/03/2014, 22:20
... membrane properties. As in past reports,
the j oint use of mutants and inhibitors acting on the same
biosynthetic points has proved to be a useful way of
distinguishing the in uence of d irect effects, ... H
+
-ATPase activity, but in
this case, it is correlated with a greater amount of enzyme
present in the plasma membranes [15]. In our case,
activation of H
+
-ATPase activity did not involve chan-
ges in ... hydrolytic activity from U. maydis plasma membrane
vesicles. Models of inhibition. H an es Plot of data obtained from wild-
type samples. Concentration of MgATP in m
M
; ATPase hydrolytic
activity in...
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Báo cáo khoa học: Kinetic modeling can describe in vivo glycolysis in Entamoeba histolytica doc
Ngày tải lên :
16/03/2014, 05:20
... a
2.3-fold increase in the flux through HK, an increase
in the glycolytic flux (58 nmol EtOHÆmin
)1
) and two-
to four-fold increase in the intermediary concentra-
tions. The HK reaction still retained ... underlying control mechanisms, a
kinetic model of the pathway was constructed by using the software gepasi.
The model was based on the kinetic parameters determined in the purified
recombinant ... concentration was also mainly exerted by ATP consum-
ing processes and 3-phosphoglycerate mutase and hexokinase (in the produc-
ing block). The model also indicated that, in order to diminish the amoebal
glycolytic...
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Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre doc
Ngày tải lên :
17/03/2014, 10:20
... working with yeast
Erv1p, this analysis was limited to six cysteines instead of
the nine present in human Alrp and in addition, the mutated
yeast proteins can be easily checked for in vivo activity.
Materials ... six cysteines in yeast
Erv1p was the major goal of this paper.
All three pairs of cysteines in Erv1p are indispensable
for
in vivo
activity
The six cysteines of yeast Erv1p can be grouped into three
pairs ... bacterial dsbB protein in the periplasm
also contains two cysteine pairs acting in concert for the
transfer of disulfides to dsbA [25]. Interestingly, the redox
function of dsbB is linked to the bacterial...
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Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
Ngày tải lên :
25/10/2012, 11:10
... characterized by insulin re-
sistance and insulin secretion deficiency. At present,
there is no a single medication which treats type 2
diabetes by improving both insulin resistance and
insulin secretion ... glycine-rich linker is flexible, which allows the
specific engineering of hinge regions into proteins to
achieve desired functional motions [17]. We used a
glycine-rich short peptide including ... fusion protein in inclusion body was
refolded in urea gradient refolding buffer. And then,
the refolded protein was incubated with enterokinase
to remove the His-tag. The fusion protein without...
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Báo cáo y học: "Relationship between Anti-CCP Antibodies and Oxidant and Anti-Oxidant Activity in Patients with Rheumatoid Arthrit"
Ngày tải lên :
25/10/2012, 11:15
... role
in RA pathogenesis. Anti-CCP antibodies may also be
related increased oxidative activity in RA patients.
Anti-CCP antibodies are directed against antigens
containing the nonstandard amino ... acid citrulline, for
example, citrullinated fibrin, which is found in the
rheumatoid joint [21]. The citrulline moiety, which is
the essential part of the antigenic determinant in these
antigens, ... peptidyl
arginine deiminases (PAD) [22]. Some reults in the
literature also suggest that anti-CCP may be related
increased oxidative activity in RA patients. For ex-
ample, TNF-alpha inhibitors...
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