in vitro studies of cell proliferation and cell death

... effects of future inhibitors designed for this NF-jB signaling pathway should be tested not just in vitro and cell based but also simulated in silico Combining this method of analysis (in vitro, cell- based ... the transcription of a large number of target genes encoding regulators of immune and inammatory responses and also genes involved in apoptosis and cell proliferation [36] In this paper, we report ... l-glutamine and 5% fetal bovine serum) and maintained at 37 C and 5% CO2 For translocation experiments, cells were removed with 0.05% trypsin EDTA, and plated with cell solution of ã 106 cellsặmL)1...

Ngày tải lên: 16/03/2014, 11:20

... harvesting complex (LHCI), consisting of four members (Lhca1–4) of the chlorophyll a ⁄ b binding protein family LHCI is bound along one side of PSI only, in the vicinity of the PSI-F and PSI-J ... extreme C-terminus of PSI-G (Fig 1) These cassettes were both cloned into binary vectors under the control of the 35S promoter and terminator, and into vectors for in vitro transcription and translation ... disrupting protein–protein interactions within PSI, or by altering the conformation of the loop His-tags were employed in a topological study of the major light harvesting chlorophyll a ⁄ b binding...

Ngày tải lên: 07/03/2014, 21:20

... during AIA in BHK cells (5 min) 149 Fig 3.58 Intracellular pH changes during AIA in BHK cells (10 min) 150 Fig 3.59 Intracellular pH changes during AIA in BHK cells (20 min) 151 Fig 3.60 Intracellular ... during AIA in BHK cells (30 min) 152 Fig 3.61 Intracellular pH changes during AIA in BHK cells (40 min) 153 Fig 3.62 Intracellular pH changes during AIA in BHK cells (50 min) 154 Fig 3.63 Intracellular ... during AIA in BHK cells (60 min) 155 Fig 3.64 Intracellular pH changes during AIA in BHK cells (90 min) 156 Fig 3.65 Intracellular pH changes during AIA in BHK cells (4 hrs) 157 Fig 3.66 Intracellular...

Ngày tải lên: 11/09/2015, 16:07

... treatment were used in the following in vivo and in vitro studies 3.3 In vitro Study To mimic the functional response of Th17 and other Th cell subpopulations to HDM allergens, an in vitro allergen ... between in vivo and in vitro findings, confirming that not only Th2, but also Th17 cells play an important role in the context of allergic inflammation IL10 producing CD4+ T cells showed a distinct ... Th17 cells mediate airway inflammation by producing its unique cytokine IL17 to induce those proinflammatory genes from both structure cells (epithelial cells and fibroblasts) and immune cells...

Ngày tải lên: 23/09/2015, 14:47

... that induce mineralization b-GP has been routinely supplemented in bone cells, chondrocytes and cultured cell types to initiate mineralization and to better monitor different aspects of mineral ... interpretation of IR spectra of mineral deposits Distinct mineralization steps were observed during the GPI-bALPinduced hydrolysis of b-GP At incubation times in the range 1.5–3 h, two bands located ... quality and the type of mineral formation obtained in the mineralization medium during the GPIbALP-induced hydrolysis of different phosphate substrates Ó FEBS 2003 Bone GPI-bALP, roles in mineralization...

Ngày tải lên: 23/03/2014, 17:21

... gniylppus rof noisiviD htlaeH laminA ,CLL erachtlaeH reyaB knaht eW noitadnuoF hcraeseR eniuqE ewoL kcaJ eht dna )TAC( noitadnuoF ygolonhceT dna ecneicS etatS kroY weN eht ,hcraeseR eniuqE rof dnuF ... ,H ihsinoK ,A orihsoK ,K aganoriH ,S eiO 62 2002 ,enyaW ,sdradnatS yrotarobaL lacinilC rof eettimmoC lanoitaN ,08-1 pp 22 loV 2A -13M ,dradnats devorppa ;slaminA morf detalosI airetcaB rof stseT ... CIM eht ,seripsotpel dna eailerrob roF dehsilbatse neeb ton sah setehcorips cinegohtap tsniaga ytilibitpecsus laiborcimitna tset ot dohtem dezidradnats dna mrofinu A aripsotpeL ortiv ni noissucsiD...

Ngày tải lên: 07/08/2014, 18:21

... INTRODUCTION Collections of seeds and clonal material are traditional ways of storing genetic resources Use of cold stored (0-10°C) in vitro cultures is a complementary method of maintaining ... (1992) Effect of in vitro storage at 4°C on surviving and proliferation of apple rootstocks Plant Cell Tissue Organ Cult 31, 1-7 ACKNOWLEDGMENTS Sánchez MC, Vieitez AM (1991) In vitro morphogenetic ... oak and chesnut cultures in cold storage for at least year without subculturing When stored after 10 d of preculture in the growth room, the proliferation capacity of the cultures was retained In...

Ngày tải lên: 08/08/2014, 19:21

... epithelial cell line, were also maintained in RPMI 1640 supplemented with 10% FBS L929, a mouse fibroblast cell line, was obtained from ECACC (number 85011425) and cells were grown in DMEM/F-12 and ... leukaemic cell line, THP-1 [17] (European Collection of Cell Cultures [ECACC], Salisbury, UK), was maintained in RPMI 1640 medium (Invitrogen Corporation) supplemented with 10% FBS The J774 cell line ... cells were grown in monolayer culture in DMEM/F12 in a fully humidified atmosphere with 5% CO2 and 21% O2 at 37°C The cells were expanded and passaged twice before use Two cell lines, THP-1 and...

Ngày tải lên: 09/08/2014, 10:23

... cautious when interpreting results obtained by using in vitro experimental model, since it can not recreate the complex interactions of cells in vivo Further limitations in this study include the ... cultured under identical conditions In brief, tissue explants were maintained in DMEM (Invitrogen, Carlsbad, CA, USA) containing 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA), 1% fungizone ... regeneration Indeed, it is well known that collagen favors the adhesion to the substrate of various cell types, permits the in vitro maintenance of cells over a long period of time and stimulates cell proliferation...

Ngày tải lên: 11/08/2014, 20:20

... 1004002041) is an epoxy resin based root canal sealer and consists of a paste-paste system, with paste A containing epoxy resin and iron oxide, and paste B containing amines and silicone oil The sealers ... release of Adenylate Kinase (AK) from damaged cells The PDL fibroblasts were incubated under standard conditions in a 96well plate (30,000 cells/well) After incubating the cells with the sealing ... homodimer II enters into cells through damaged membranes, binding to nucleic acids, thereby producing a bright red fluorescence in dead cells The intact cell membrane of live cells is not permeable...

Ngày tải lên: 11/08/2014, 20:21

... injury were first incubated for 30 minutes at 37°C, 95% air and 5% CO2 in EM containing 4.5 μM PI Following baseline fluorescence imaging the traumatic brain injury was generated using an apparatus ... phosphoinositide 3-kinase/protein kinase B cascade [56] These findings can be a starting point for further studies to describe argons definite mode of action Nevertheless it is surprising that ... two and three hours after injury Discussion We investigated the potential neuroprotective effects of the noble gas argon in two in vitro models of OGD and TBI Our methods involved depriving cultured...

Ngày tải lên: 13/08/2014, 20:21

... anchored in the envelope, functions as an ion channel between the interior of virus and its environment and maintains a low pH during virion uncoating during initial stages of infection (Pinto, Holsinger ... increasing the likelihood of a pandemic 1.4 Clinical manifestations and pathology of influenza infection Influenza viruses can infect people of all age groups In addition, infants, the aged and ... embryonic stem cells FBS foetal bovine serum g gram h hours xv H&E haematoxylin and eosin stain HRP horseradish peroxidase ICE interleukin-1β-converting enzyme IFN interferon IL interleukin INF infected...

Ngày tải lên: 13/10/2015, 16:41

... gniniatnoc pord muidem noitalupinam a otni derrefsnart dna ,muidem noitalupinam hserf ni eciwt dehsaw ,)24333 tshceoH lm/gµ gniniatnoc ASB-HLT eerf-muiclac( muidem noitalupinam ni nim 51 rof ... ,h rof edimixeholcyc lm /gµ 01 gniniatnoc muidem CVI ni detabucni erew setycoo detalumits-ortcelE 2lCgM Mm 50.0 dna 2lCaC Mm 10.0 gniniatnoc lotinnam Mm 082 ni ces yb detarapes cesµ 06 rof V ... )v/w( %4.0 gniniatnoc muidem 32 -)USCN( ytisrevinU etatS aniloraC htroN saw tnempoleved oyrbme rof muidem )CVI( erutluc ehT stnemirepxe lla ni desu saw hctab emas eht morf diulf ralucillof enicroP...

Ngày tải lên: 07/08/2014, 20:23

... bovine serum (FBS, Sigma, Japan) and 1% antibiotics and antimicotics (Gibco, Japan), and they were maintained at 37°C in a humidified atmosphere of 5% CO2 in air U-CH1-N cells and cell doubling ... this is the first report presenting the extensive in vitro cellular studies including radiation and chemical cell survival/ toxicity curves with the cell line originating from chordoma Acknowledgements ... bleocin, B) cisplatin, C) mitomycin C, and D) camptothecin Black square represents HeLa cells, white triangle indicates U87-MG cells, and black reverse triangle indicates U-CH1-N chordoma cell line...

Ngày tải lên: 09/08/2014, 09:21

... to cell cycle arrest, 4-HPR and both of the iodo-derivatives induced high levels of cell death in all RT cell lines tested The most dramatic induction of cell death occurred in G401 cells, in ... has resulted in prolonged stabilization of disease in pilot clinical studies [17-20] 4-HPR induces apoptosis in tumor cells in vitro by various mechanisms including activating retinoic acid receptors ... significantly induced cell death in all cells lines (P

Ngày tải lên: 13/08/2014, 18:21

... protein, as seen in HCT116 cells, resulted in the absence of enhanced cell growth inhibition The central role of p21waf1 in mediating senescence is currently being investigated in isogenic cell lines ... growth arrest and suppression of invasiveness by CRE-decoy oligonucleotide in ovarian cancer cells: protein kinase A downregulation and cytoplasmic export of CREbinding proteins Mol Cell Biochem ... fetal bovine serum HCT116 and GEO cell lines were both wild-type p53, and SW620 lines were mutant p53 No antibiotics were used in our experiments, and all cell lines were incubated in a humidified...

Ngày tải lên: 07/03/2014, 15:20

... TNF-α in triggering and modulating apoptosis is less clearly defined This is because, in addition to signalling through the death domain of TNFRI, TNF-α activates mainly signalling pathways and ... Briefly, following induction of apoptosis, cells were fixed in 1% paraformaldehyde and incubated with Br-dUTP in the presence of TdT enzyme, which results in the incorporation of Br-dUTP into exposed ... susceptibility to FasL-induced cell death, and TNF-α prevented these cells from undergoing FasL-induced cell death in a dose-dependent manner No such inhibitory effects of TNF-α on FasL-induced apoptosis...

Ngày tải lên: 09/08/2014, 01:23

... protein tyrosine- and histidine kinases [810], and inhibits topoisomerase I and II [11] These effects result in alterations of several intracellular and extracellular pathways including cell ... radiosensitivity in the androgen independent PC cell line PC-3 has been demonstrated in vitro and in vivo [26,27] Our study analyzes the interactions of irradiation and genistein or estradiol incubation in androgen ... tumor cell lines in vitro: in human esophageal squamous cell cancer cell lines (TP 53 mutant and wild-type) [21], hepatoma cells [22], leukemia cells [23], and PC cell lines [24,25] Furthermore, increased...

Ngày tải lên: 09/08/2014, 09:22

... (H) and (I) Histochemical analysis of GUS expression in pVvMYB60:GUS leaves in response to ABA (H) GUS staining of stomata in a control leaf (I), GUS staining of stomata following hours of exposure ... our findings indicate a role for VvMYB60 in the regulation of guard cell activity and transpiration rate in grapevine Stomatal conductance is a key trait in grapevine, as it directly determines ... in a sealed chamber in the presence of 100 ml of commercial bleach and ml of 37% HCl, and germinated in Petri dishes containing Murashige and Skoog medium, 1% w/v sucrose and 0.8% w/v agar Plants...

Ngày tải lên: 11/08/2014, 11:21

... obtained after incubating cells with GSH (1.25 – mM) instead of NAC (data not shown) In contrast, in NCI-H292 cells but not in PBEC, depleting GSH using BSO resulted in a full inhibition of wound ... medium containing glutamine, penicillin and streptomycin After washing with ice-cold PBS, stimulated cells were lysed with reducing sample buffer and incubated for 10 minutes on ice Proteins were ... CSC proliferation and its modulation by BSO and NAC on cell Effect of1 in NCI-H292 Effect of CSC and its modulation by BSO and NAC on cell proliferation in NCI-H292 Subconfluent cultures of NCI-H292...

Ngày tải lên: 12/08/2014, 18:20