in vitro adult canine adipose tissue derived stromal cell growth characteristics

Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc

Ngày tải lên : 30/03/2014, 04:20

... apolipoprotein A-I apolipoprotein A-II apolipoprotein B apolipoprotein C-I apolipoprotein C-II apolipoprotein D apolipoprotein E apolipoprotein F apolipoprotein H fatty acid binding protein 1, liver ... 1.00 ATP-binding cassette, sub-family transcript variant ATP-binding cassette, sub-family ATP-binding cassette, sub-family transcript variant ATP-binding cassette, sub-family ATP-binding cassette, ... factor in determining stem cell transdifferentiation Discussion AT-MSCs may be good candidates as stem cells for cell transplantation and tissue engineering in regenerative medicine, as a large...

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Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx

Ngày tải lên : 07/08/2014, 23:22

... New canine spinal cord injury model free from laminectomy Brain Res Brain Res Protoc 2005, 14, 171-180 11 Gimble J, Guilak F Adipose- derived adult stem cells: Transplantation of ASCs in spinal ... umbilical cord blood derived MSCs was effective in canine SCI model, there was little histological evidence of spinal cord tissue regeneration [22] In this study, we examined whether canine ASCs could ... tissue- derived stromal cells after cerebral ischemia in rats Exp Neurol 2003, 183, 355-366 Kang SK, Shin MJ, Jung JS, Kim YG, Kim CH Autologous adipose tissue- derived stromal cells for treatment of spinal...

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Investigation of osteogenic characteristics of human adipose derived stromal cells

Ngày tải lên : 08/11/2015, 17:01

... Mohan C Abraham SUMMARY Adipose tissue is being considered as having a potential source for Mesenchymal stromal cells (MSCs) known as Adipose Derived Stromal Cells (ADSCs) In this work, ADSCs were ... to spindle shaped on continued culture (Fig 4.1B) Fig 4.2 Alizarin Red staining of ADSC The uninduced samples (Fig 4.2A) not take up any stain Intense foci of mineralization seen in the induced ... precursor cells for this purpose The two types of stem cells that are being targeted for use in tissue regeneration are the Embryonic stem cells and the Adult stem cells The embryonic stem cells, being...

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Báo cáo y học: " High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells." ppsx

Ngày tải lên : 10/08/2014, 10:20

... these cells These data will significantly contribute to tissue engineering by providing abundant seeding cells with high quality List of abbreviations ADSCs: adipose- derived mesenchymal stem cells; ... mesenchymal stem cells; ALP: alkaline phosphatase; DT: doubling time; EGM2-MV: endothelial cell growth medium 2; FACS: fluorescein-activated cell sorting; FBS: fetal bovine serum; FITC: fluorescein isothiocyanate; ... Hyclone, USA) containing 10% fetal bovine serum (FBS, Invitrogen, USA) Dissociated tissue was filtered to remove debris, and centrifuged at 1500 rpm for 10 The suspending portion containing lipid droplets...

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Characterization of human adipose tissue derived adult multipotent precursor cells

Ngày tải lên : 12/09/2015, 09:55

... Chen Fulin Polysaccharides in Tissue Engineering Applications, Handbook of Carbohydrate Engineering In press Intellectual Competition Finalist in Tan Kah Kee Young Inventors Awards 04 Making bone ... Characterization of cells in in vitro culture plastic environment • Characterization of cells in in vitro 3-D environment • Characterization of cells in in vivo animal models • An in- depth analysis ... correspondingly true in humans There is a possibility of a difference within the human and mouse stem cell systems, as seen in the ability of murine leukaemia inhibitory factor (LIF) in maintaining...

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Báo cáo y học: " Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells" pdf

Ngày tải lên : 13/08/2014, 13:20

... ICAM-3 grabbing nonintegrin) is a 44-kDa type I membrane protein with an external mannose-binding, C-type lectin domain [42] It has been postulated that DC-SIGN interacts with ICAM-3 on T cells to ... Th functions and intracellular cytokine staining The in vitro DC:T cell co-culture method was modified based on Caron et al[26] Briefly, we co-cultured purified naïve CD4 T cells with allogeneic ... T cells were purified based on negative selection of CD45RO- cells using the MACS (Miltenyi Biotec, Auburn, CA) magnetic affinity column according to the manufacturer's instructions In vitro induction...

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Báo cáo y học: " Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions" pps

Ngày tải lên : 14/08/2014, 08:21

... 4',6-diamino-2phenylindole; GO, Gene Ontology; GSEA, gene set enrichment analysis; IGF, insulin-like growth factor; IGFBP, IGF binding protein; IL, interleukin; JAK, Janus activated kinase; KEGG, ... PTL induces apoptosis in primitive cancer cells PTL induces apoptosis in primitive cancer cells Percent viability of prostate cancer cells and cells from a patient with BPH treated with increasing ... adhesion kinase through α5β1 integrin/fibronectin has previously been implicated in regulating the invasiveness of prostate cancer cells via activation of phosphatidylinositol-3,4,5trisphosphate kinase...

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Engineering and characterization of human renal proximal tubular cells for applications in vitro toxicology and bioartifical kidneys

Ngày tải lên : 09/09/2015, 10:19

... origin such as Madin-Darby canine kidney (MDCK) cells were used (75) There are challenges associated with extrapolating results obtained with animal cells /cell lines Animal cells /cell lines (MDCK/LLC-PK1) ... in vitro applications The underlying reason behind such a great interest in tissue models that contain more than one cell type is that a multiple cell type-system enables the study of cells in ... with other cells in the renal cortex in producing or responding to costimulatory cytokines, i.e tumour necrosis factor alpha(35) In addition, PTC produce interleukin-6 in response to inflammatory...

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ENGINEERING THREE DIMENSIONAL CULTURE PLATFORMS FOR HUMAN ADIPOSE DERIVED STEM CELL THERAPY

Ngày tải lên : 09/09/2015, 11:18

... harvest tissues by minimally invasive means which contain large numbers of stem cells 2.2.3 Adipose derived stem cells ADSCs are adult stem cells found in adipose tissues with very similar characteristics ... ADSCs ADSC-GMs Adipose derived stem cells Cell- microsphere constructs formed using adipose derived stem cell and gelatin microspheres AFP Alpha-fetoprotein ALB Albumin ALP Alkaline phosphatase ... value for treating organ failures Adipose derived stem cells (ADSCs) which are present in adipose tissue are one such kind of adult stem cells They are categorized as mesenchymal stem cells (MSCs)...

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Báo cáo khoa học: "Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose-tissue-derived stem cells: a case series" docx

Ngày tải lên : 10/08/2014, 23:21

... PRP contains multiple growth factors including transforming growth factor (TGF)b, insulin-like growth factor (IGF), fibroblast growth factor (FGF), and platelet -derived growth factor (PDGF) A ... brought into an operating room and placed in a supine position She was then sedated with propofol mg intravenously (push) and a 20 mg/hour rate of continuous infusion After cleaning her abdominal ... maceration After obtaining ADSCs and preparing PRP as described above for the first two patients, she was prepared for injection of the mixture into the joint In order to inject the stem cell and PRP...

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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx

Ngày tải lên : 18/06/2014, 19:20

... modulatory cytokines for groups of MDSC-inducing and non-inducing human cancer cell lines [16] These studies suggested multiple mechanisms of MDSC induction amongst tumor cell lines, including inflammatory ... of in vitro- generated MDSC was examined for expression of myeloid, antigen-presenting, and suppressor cell markers For staining, cells were collected from flasks using Detachin to minimize cell ... Canonical CD33+ human MDSC induction by human cancer cell lines Inducing Tumor Cell Line Mean Percent Suppression SEM Inducing Tumor Cell Line Controls T cells alone 0.00 Medium only -2.35 0.86 Lung...

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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx

Ngày tải lên : 13/08/2014, 23:20

... Ratio of inner cell mass and trophoblastic cells in blastocysts derived from porcine 4- and 8cell embryos and isolated blastomeres cultured in vitro in the presence or absence of protein and human ... parts, it has been suggested that the defining vital characteristics of a 4- or 8 -cell human embryo must include continued and integrated cellular division, growth, and differentiation [16] And by ... suffer from life destroying diseases Only certain cell lines will be considered eligible for federally-sponsored human ESC research These cell lines must be derived (1) with the informed consent of...

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Immunological characterization of human umbilical cord lining derived cells and their therapeutic application in a diabetic mouse model

Ngày tải lên : 11/09/2015, 10:02

... of cord lining cells in vivo using an immunocompetent mouse model To test the therapeutic application of cord lining cells in ex vivo insulin gene therapy using a streptozocin (STZ) induced type ... stem cell IACUC institutional animal care and use committee ICAM intercellular adhesion molecule IFN interferon IHC immunohistochemistry IL interleukin INS insulin IP intraperitoneal IRB institutional ... differentiated into insulin producing clusters Successful applications of those MSC -derived insulinproducing cells in treatment of diabetic animals have also been reported Problems that remain difficult include...

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báo cáo hóa học:" Human fallopian tube: a new source of multipotent adult mesenchymal stem cells discarded in surgical procedures" potx

Ngày tải lên : 18/06/2014, 15:20

... cells induced for myogenic differentiation 1A) Dystrophin expression in muscle control and in the induced hFTs cells 1B) Skeletal myosin expression in muscle control and in the induced hFTs cells ... cells 1C) Myosin band observed in the muscle control and in the induced hFTs cells by Ponceau S membrane dyeing 1D) IF assay, indicating dystrophin expression (in green fluorescence) in myotubes ... using stem cells for regenerative medicine has opened a new field of investigation to find the best sources for obtaining multipotent stem cells, in particular through non-invasive procedures Initially...

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Báo cáo y học: "Human infrapatellar fat pad-derived stem cells express the pericyte marker 3G5 and show enhanced chondrogenesis after expansion in fibroblast growth factor-2" potx

Ngày tải lên : 09/08/2014, 10:23

... particular benefit in increasing the production of matrix in cartilage tissue- engineered in vitro The inhibition of actin stress fibres by FGF-2 in adult human chondrocytes results in an upregulation ... vasculature 3G5 (a, b) staining predominantly the tunica adventitia consisting of supporting tissue in the vasculature, vWF (c, d) staining predominantly the tunica intima consisting of the endothelial ... the cells was determined by staining with Trypan blue Isolation of clonal populations Clonal cell populations were derived from single cells obtained by limiting dilution Freshly isolated cells...

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Báo cáo y học: "Isolation and characterization of human cells resistant to retrovirus infection" pdf

Ngày tải lên : 13/08/2014, 05:22

... the hypoxanthine guanine phosphoribosyl transferase (HPRT) locus and at the adenine phosphoribosyltransferase locus by plating in medium containing 6-thioguanine (6-TG) or diaminopurine (DAP), ... in Figure The range is considered linear where increase in moi yields a corresponding increase in the number of cells infected and the geometric means of fluorescence (a measure of multiple infections) ... import Methods Tissue culture 293T cells, HeLa and derived cell lines (30-2 and 42-7) were maintained in Dulbecco's modified Eagle's medium, DMEM (Cellgro) supplemented with 10% Fetal Bovine serum,...

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Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"

Ngày tải lên : 25/10/2012, 11:10

... the loading of pravastatin into erythrocytes was directly proportional with increase of drug concentration in the incubation medium, in the 2-10 mg/ml concentration range This finding is in concurrence ... Pharmacokinetic-pharmacodynamic drug interactions with HMG-CoA reductase inhibitors Clin Pharmacokinet 2002; 41(5):343-370 Hatanaka T Clinical pharmacokinetics of pravastatin: mechanisms of pharmacokinetic ... pravastatin to the total amount of that added during the entire loading process Cell recovery The percentage ratio of the hematocrite value of the final loaded cells to that of the initial packed cells,...

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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx

Ngày tải lên : 19/02/2014, 02:20

... of Huntington’s disease are duplicated in experimental animals by intrastriatal quinolinate injection [5,6] In addition, a signiﬁcant elevation of quinolinate levels has been observed in low-grade ... corresponding amino acids in the human protein are pointed with arrows The mean hydrophobicity values of residues within the window centered on the pointed amino acids are reported in brackets ... al hydrophobic index of the residues within the window centered on amino acids involved in metal binding, it can be noticed that regions around His6 and His8 in the human protein are predicted...

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