in situ bioreclamation of contaminated groundwater

... distributions of in situ nitrifying activities were determined The relationship between the spatial organization of nitrifying bacterial populations and the in situ activity of these populations within ... were performed In situ hybridization of vertical biofilm thin sections clearly indicated that the numbers of probe NEU-stained NH4+-oxidizing bacteria (i.e., Nitrosomonas marina-lineage, Nitrosomonas ... distributions of nitrifiers and heterotrophs in mixed population biofilms Biotechnol Bioeng 50:24-35 Okabe, S., H Satoh, and Y Watanabe 1999a In situ analysis of nitrifying biofilms as determined by in situ...

Ngày tải lên: 05/09/2013, 08:40

... Incubate while rocking for h at room temperature in the dark in 100 µL of staining buffer containing 10 µg/mL of FITC-conjugated streptavidin Wash by diluting the sample with mL of 2× TB buffer ... counterstain Incubate cells in 0.2 µg/mL DAPI, and rinse once in PBS To label fixed samples using both TUNEL and immunofluorescence staining for an antigen of interest, the antibody incubations ... used methods for detecting DNA damage in situ is TdT-mediated dUTP-biotin nick end labeling (TUNEL) staining (1) TUNEL staining was initially described as a method for staining cells that have undergone...

Ngày tải lên: 11/04/2014, 09:47

... order of 1–10 ns [6], which makes the formation of homogeneous nuclei unlikely during heating with laser pulses less than ns This work addresses these issues by performing timeresolved imaging of ... light to a wavelength of 532 nm Any light remaining at the fundamental wavelength is removed in an infrared absorbing filter The probe beam then travels through a retroreflecting prism that is mounted ... delay of 20 ps This attenuated region increases to a diameter of 64 mm at 100 ps, with some fluctuations in diameter due to changes in pump beam irradiance The level of attenuation also increased...

Ngày tải lên: 06/05/2014, 08:55

... line in Fig 4g) The latter is caused by the surface roughening of the 2D Ge wetting layer surface associated with the formation of a high density of dimer vacancy lines and subsequently missing ... facet spots sharpen and strongly increase in intensity This indicates an initial rapid expansion, i.e., growth of the {113} sidewall facets of the stripes During further Si growth at 520°C, however, ... improves and the initial 3D diffraction spots completely disappear (see Fig 1b) Continuation of buffer growth, results in the appearance of faint additional diffraction streaks inclined by 25° to...

Ngày tải lên: 21/06/2014, 08:20

... Foundation of China (50702017), the Innovation Foundation of Harbin Institute of Technology (HIT NSRIF 2008.51) the Post-Doctor Foundation (20060390786), and the program of excellent team in Harbin Institute ... result from TEM Principle of In Situ Mineralization of Magnetite in Chitosan Hydrogel Fig TG curves of pure magnetite (a) and the mineralized magnetite nanoparticles (b) existence of chitosan layer ... Hysteresis loop of the mineralized magnetite nanoparticles at 300 K 123 1046 Nanoscale Res Lett (2009) 4:1041–1046 Fig Principle of in situ mineralization of magnetite nanoparticles in chitosan hydrogel...

Ngày tải lên: 21/06/2014, 20:20

... Encapsulation of bFGF in MSNs A weak acidic water -in- oil method followed by ammonium hydroxide treatment was developed to encapsulate bFGF in situ Figure illustrates the in situ loading of bFGF in MSNs ... concentration of released bFGF in PBS at predetermined intervals of time C0 is the total concentration of released bFGF from MSNs In addition, the loading efficiency (R) of bFGF describes the capacity of ... activation of protein kinases [26] Meanwhile, our study indicated that there is no obvious increase of dead cell (% of control) in the cytotoxicity assay during the test period The results indicate...

Ngày tải lên: 22/06/2014, 00:20

... discussion of AFM results points to the possible core–shell structure formation during in situ synthesis of Au–Pt NPs on the surface from the observed increase in nanoparticle size going from pure ... mixed in different volumes to create the desired Au:Pt mole ratio in the final solution, ranging from 1:0,1:0.25, 1:0.75 to 1:1 After incubating the surface for h, rinsing with water and drying ... analysis software Surface coverage of different nanoparticles was estimated by analyzing the mean grain area and number of grains present in a typical lm lm (total area = lm2) AFM image of the respective...

Ngày tải lên: 22/06/2014, 00:20

... patterns in the nanofibers were due to the small size of the crystal [48] Conclusions We prepared two kinds of the nanocomposites by using an in situ polymerization method in the presence of the ... demonstrates that 4-aminobenzoyl moiety was covalently attached to the surface of MWNT as shown in Scheme [36] The aromatic amine groups will provide the sites for the ring opening initiation of e-caprolactam ... Res Lett (2009) 4:39–46 Fig FT-IR spectra of (a) P-MWNTs and (b) F-MWNTs In situ Polymerization In situ polymerization of e-caprolactam in the presence of the F-MWNTs and the P-MWNTs was carried...

Ngày tải lên: 22/06/2014, 01:20

... be induced by: 1) individual variability 2) changes in A between morning and evening at the same level of incident and PPFD The maximum values for potato about the same as those for lilac are increases ... is the point where the maximum of demand function crosses the C;regulation line We can observe that, if WUE of maize is higher than the WUE of lilac or potato, the junction occurs within the same ... Up to and 0.23 , s m’ mol the g dependence of A is c c almost linear and the slope of this line represents C Beyond these values, i - C a A does not increase for both C plants, although g can...

Ngày tải lên: 09/08/2014, 04:20

... prostacyclin release was not involved in vasodilatation of rat pulmonary artery In contrast, in guinea-pig lung slices, indomethacin increased dilator responses, possibly via the inhibition of thromboxane ... (NOS) inhibitor L-NGnitro-L-arginine methyl ester (L-NAME; 10-3 M), the combined cyclo-oxygenase-1/ cyclo-oxygenase-2 inhibitor indomethacin (10-5 M) or the combination of apamin and charybdotoxin ... in lung slices the inhibitory effects we see with L-NAME are via the selective inhibition of NOSIII in the endothelium Our results showing that the combination of apamin plus charybdotoxin (inhibitors...

Ngày tải lên: 12/08/2014, 16:20

... verifying that nonspecific binding of the Qdot 655 multimer is low Staining pattern and staining intensity of Gag CM9 Qdot 655 multimer positive cells Confocal microscopy revealed a punctate staining ... ranging from 2.43%- 9.59% of all CD8+ cells, confirming reports using flow cytometry or in situ staining of fresh tissues using tetramers [10,24-28] We also looked for CD8+ T cells recognizing ... useful in undertaking a detailed in situ analysis of the CD8+ T cell responses in SIV infection Conclusion In this study we demonstrate that by using the Gag CM9 Qdot655 multimer instead of Gag...

Ngày tải lên: 12/08/2014, 23:23

... G.3 Far-infrared Region A representative spectra of the far-infrared region, 400-700 cm-1, are shown in Figure G.1 Figure G.1 In- situ raw far-infrared spectra of the hydroformylation of 3,3-dimethyl-1-but-ene ... the highpressure cell were recorded Hydroformylation reaction was initiated by introducing MPa of H2 gas Then, spectra were recorded at 10 minute intervals in the range 400-2500 cm-1 The semi-batch ... Preliminary FIR Analysis Appendix G Preliminary FIR Analysis The current appendix presents the first attempt of analyzing the far-infrared region The collected spectra were obtain from monitoring...

Ngày tải lên: 14/09/2015, 12:54

... achieved by a single individual, therefore, I was very fortunate to be part of very competent team at the National University of Singapore and Institute of Chemical and Engineering Sciences (ICES) ... change of a reaction s Number of species T Temperature in Kelvin T1× z Transformation matrix U ∞ v CO V ν s×E Matrix of left singular vector Partial molar volume of the dissolved CO gas at infinite ... of the collected in- situ spectra, (4) a sound understanding of organometallic chemistry in particular coordination chemistry (5) the use of density functional theory to assist in geometrical assignments...

Ngày tải lên: 14/09/2015, 13:04

... BUILT -IN SENSOR ARRAY FOR IN- SITU MEASUREMENT OF CELLULAR MICROENVIRONMENT ZHANG LIN (B.Eng, ZJU, China) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF ENGINEERING DEPARTMENT OF BIOENGINEERING ... encapsulation of these sensors β-TC-6 cell was used as the cell line cultured in the final microfluidic device and in- situ measurement of three parameters including pH, oxygen and glucose concentration in ... showing the sensors’ robustness in working in the real cell culture environment The measurement is in- situ, real time and reagentless, fulfilling all requirements of microscale sensing Cells in...

Ngày tải lên: 03/10/2015, 11:36

... systems and the number of sets for each This helps inducing the density of fissuring, which increases the heating rate Delineating the alignment and dimensions of faults Constructing stratigraphic ... illustrations of rock successions, as needed Identifying the characteristics of aquifeours strata and the hydrogeologic setting of the springs In principle field testing is applied in- situ, and primarily ... determined in the field reconnaissance The following points must be taken into account while using thermal detectors to measure temperature of water sources: The increase in temperature of water...

Ngày tải lên: 05/09/2013, 16:10

... characterization of the initial acetylene hydratase is inevitable In case of the other, more common enzymes, which are involved a genomic sequence analysis and a comparison to the genome of other strains ... by in situ 1H-NMR analysis (1H d: 3.06 p.p.m) in 1H2O Spectra indicated the additional presence of a small amount of 3-ketopropanoate (1H d: 3.48 p.p.m) and acetaldehyde (1H d: 1.17 p.p.m) during ... metabolise this substrate in a different way Performing the propynoate biotransformation in 1H2O/ D2O (1 : 1) led to an incorporation of 50% deuterium in all metabolites The addition of 0.5 mM acetaldehyde...

Ngày tải lên: 08/03/2014, 08:20

... X Ding et al / Journal of Colloid and Interface Science 320 (2008) 341–345 by oxidatively polymerizing aniline using ammonium peroxodisulfate as oxidant (NH4 )2 S2 O8 [23] There, the obtained ... aggregate in the presence or even the absence of a magnetic field Furthermore, the major difficulty lies in the interrelated effects in synthesis and assembly Agglomeration of these particles originates ... synthesized using the HCl as dopant The DBSA-doped PANI with nanorods structure was synthesized as follow In a typical experiment, ml of DBSA and 0.093 g of purified aniline were dissolved in 50 ml of distilled...

Ngày tải lên: 20/03/2014, 13:04

... cooperatively in the cross-linking of proteins, including involucrin, loricrin and small Preferred substrate peptide for TGase proline-rich proteins (SPRs) Such concerted reactions result in formation of ... consisting of covalent crosslinks of proteins beneath the plasma membrane of terminally differentiated keratinocytes [23,24] Furthermore, TGase in hair follicles is involved in crosslinking structural ... for detecting in situ activity of TGase in a frozen mouse skin section As shown in Fig 6, in the presence of CaCl2, specific incorporation of FITC–pepE51 (1 lm) in endogenous glutamine-acceptor...

Ngày tải lên: 29/03/2014, 21:20

... semiquantitatively for cytoplasmic staining on computer screen The dominant staining intensity in tumor cells was scored as: = negative; = weak; = intermediate; = strong (Figure 1) In case of disagreement (score ... now being exploited in cancer, attempting to modulate their expression, reintroducing microRNAs lost in cancer, or inhibiting oncogenic microRNAs by using anti-micro oligonucleotides [38] In a ... Hospital of North Norway, Tromso, Norway 2Institute of Clinical Medicine, University of Tromso, Tromso, Norway 3Department of Pathology, University Hospital of North Norway, Tromso, Norway 4Institute...

Ngày tải lên: 18/06/2014, 16:20

... rodent spine in flexion-extension testing Incorporating the same methodology used in human spine testing, we were able to assess spinal fusion in the rodent model In humans, quality of spinal fusions ... bleach Page of After 45 minutes, 6lb fishing line was threaded through the spinal canal of the sample Samples were then placed into a tray and covered before submerging in bleach again for more ... to determine optimal spinal flexion/extension conditions for testing fusion integrity Maximal intervertebral motion of untreated spines was observed at 150 of spinal flexion/extension Intervertebral...

Ngày tải lên: 20/06/2014, 04:20