implications for development of cell therapy protocols for myocardial infarction

... shows the use of the regulatory sequences of the H19 gene for the development of DNAbased therapy for human ovarian cancer related ascites The successful development of anti-tumor gene therapy depends ... Determination of the level of RNA products of the H19 gene The PCR reactions were carried out in 25 μl volumes in the presence of ng/μl of each of the forward and the reverse primers using 0.05 units/μl of ... intensity of hybridization signal was indicated as (+1) for weak, (+2) for moderate and (+3) for strong signals The quantity of the staining was referred to (+1) up to one third of the cells, (+2)...

Ngày tải lên: 18/06/2014, 15:20

... of 94°C for 30 sec, 59°C for 45 sec and 72°C for 60 sec, and finally 72°C for The PCR reactions were carried out in 25 μl volumes in the presence of ng/μl of each of the forward and the reverse ... indicated as (0) for no staining, (+1) for weak, (+2) for moderate and (+3) for strong signals The distribution of the hybridization signal was referred to as up to one third of the cells, + (1), ... provides a more relevant tool for the investigation of the biology and therapy of bladder cancer than subcutaneous implantation of bladder cancer cells (heterotopic model) Therefore, a mouse model was...

Ngày tải lên: 18/06/2014, 16:20

... submitted for the degree of Doctor of Philosophy in the Graduate Program in Bioengineering at the National University of Singapore under the supervision of Professor Lee Chuen Neng, Professor Teoh ... 1-3: Endothelial cells derived from adult stem cell sources and the method of induction 30 Chapter Introduction The harvest of bone marrow derived stem cells for autologous therapy eliminates ... substrate cues for modulating cell responses The development of microthin polycaprolactone (PCL) films raised the possibility of using these films as scaffolds to complement the cell sheet approach...

Ngày tải lên: 14/09/2015, 08:49

... Chapter Isolation and Characterisation Of Vascular Progenitors Figure 4-3: Primary culture of haemopoietic tissue in EGM Following culture in endothelial cell media, outgrowth colonies were generated ... (a) umbilical cord blood mononuclear cells with typical cobblestone morphology Similarly, outgrowth cells could be generated from (b) fetal blood mononuclear cells, which appear to have a more ... same medium, fibroblastic cells quickly grew out from fetal liver mononuclear cells, and failed to express endothelial phenotype 122 Chapter Isolation and Characterisation Of Vascular Progenitors...

Ngày tải lên: 14/09/2015, 08:49

... GO:45664: regulation of neuron differentiation GO:8361: regulation of cell size GO:16049: cell growth GO:1502: cartilage condensation GO:1558: regulation of cell growth GO:48627: myoblast development ... of the cells to semi-solid cultures for haematopoietic colony forming assays as an assessment of the retention of haematopoietic character following expansion As seen in Figure 4-10, Colony forming ... forming assays of expanded FL133+ cells generated CFU-E (Colony Forming Unit Erythrocyte, 1.33/103 cells), BFU-E (Burst Forming Unit Erythrocyte, 3.00/103 cells), CFU-M (Colony Forming Unit Macrophage,...

Ngày tải lên: 14/09/2015, 08:49

... the adhesion of perivascular cells to modified PCL film surfaces for the generation of the perivascular compartment I then proceeded to engineer a surface for the reconstitution of the tunica ... on cell adhesion, as well as blood compatibility Finally, I studied the assembly of cells and film to form a layered structure The tunicae in vascular tissue are unique in the composition of cells: ... poor cellular adhesion and reduced viability of the anchorage dependent cells (Vogler 1998) To overcome issues of poor adhesion and poor proliferation of EPC on µXPCL-PAAc, I studied the use of...

Ngày tải lên: 14/09/2015, 08:49

... Thrombolysis in Myocardial Infarction [TIMI] flow grade of or 1) on angiography, death from any cause before angiography could be performed, or recurrent myocardial infarction before angiography ... guidelines for the management of patients with ST-elevation myocardial infarction: a report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines for the ... history of myocardial infarction A total of 99.7 percent of the patients received a fibrinolytic agent, of whom 68.8 percent received a fibrin-specific agent The median time from the onset of symptoms...

Ngày tải lên: 27/06/2014, 00:20

... immediately before the onset of NHL An important feature of our cohort was the finding of symptomatic NHL occurring in the setting of profound HIV viral load decline following initiation of HAART ... Week (± 1) CD4 Not performed Not performed 234 195 Not performed Week 12 (± 2) CD4 15 250 40 33 Week 24 (± 2) CD4 Not performed 112 294 Not performed (deceased) Not performed Baseline HIV viral ... regimens Huhn et al AIDS Research and Therapy 2010, 7:44 http://www.aidsrestherapy.com/content/7/1/44 Page of Table Antiretroviral use and risk ratios for development of NHL in subjects after starting...

Ngày tải lên: 10/08/2014, 05:21

... DEVELOPMENT OF NEW HUMAN STEM CELLDERIVED CELLULAR VEHICLES FOR GLIOMA GENE THERAPY ZHAO YING (B Sc., PKU; M Sc., PKU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ... remains one of the most lethal forms of cancer in humans However, current therapy for glioma rarely achieves long-term tumor control Stem cell based gene therapy is a promising new strategy for the ... (Galli et al, 2004) Studies of the origin of brain cancer stem cells reveal that they arise from the malignant transformation of normal somatic stem cells or of more mature cells within the high-proliferation...

Ngày tải lên: 11/09/2015, 09:00

... in Ghana: Implications for Development and Performance of the Industry By William F Steel & David O Andah June 2003 Foreword his country study is one of three being published as part of research ... of research on the implications of legal and regulatory structures for the development of microfinance institutions in African countries This research is a collaborative effort between the World ... be a flexible means of regularizing three types of MFIs through: • transformation of NGOs into licensed financial intermediaries; • formalization of actual or potential informal money- lending...

Ngày tải lên: 22/03/2014, 21:20

... of action of ethambutol Recent studies have confirmed that decaprenyl-phospho-arabinose is the only donor of the arabinofuranosyl residues for the synthesis of the cell- wall arabinogalactans of ... scheme of biosynthesis of D-arabinofuranosyl residues of the cell- wall arabinogalactan and lipoarabinomannan in mycobacteria, including a feedback mechanism and possible sites of action of ethambutol, ... beginning of a fruitful search that has led to the identification of the d-arabinose pathway, and to a better understanding of cell- wall biosynthesis and of the mechanism of action of ethambutol...

Ngày tải lên: 30/03/2014, 04:20

... T, Kopp W, Mackall CL, Berzofsky J, Read EJ: Development of a clinical-scale method for generation of dendritic cells from PBMC for use in cancer immunotherapy Cytotherapy 2001, 3:19-29 10 Berger ... patterns: down-regulation of groups of miR at hours of maturation with sustained low expression throughout the rest of maturation and up-regulation of other groups of miR at hours of maturation and sustained ... These Treg cells can counter the effects of Th1 responses by cytotoxic T cells, Th1 cells, and NK cells In contrast, we found that LPS and IFN-g maturated DCs did not increase the levels of CCL22...

Ngày tải lên: 18/06/2014, 16:20

... Program for the development and elaboration of this essay These concepts were originally presented at the 2nd "Cellular Therapy of Cancer" Symposium of the ATTACK (Adoptive engineered T -cell Targeting ... descriptions could be made for control of genes desirable for expression (e.g., of cytokine) that is time-limited without terminating the T cells, allowing for resumption of activity at a later time ... types of discussion as for the Igbased CAR constructs Bearing in mind that there is a 100-fold expansion of T cells for the lowest useful doses in the engraftment protocols (e.g., 109 cells)...

Ngày tải lên: 18/06/2014, 16:20

... treatment Helper T -cell (Th cell/ CD4) % of total T cells 114 48.84 (9.03) 45.44 (10.65) 28-58

Ngày tải lên: 18/06/2014, 16:20

... of clinical situations to validate the rules implemented and to detect any bugs Figure Example of a platform for development of an explicit computerized protocol dedicated to the management of ... patients used for ECP development are computer simulations of the physiologic processes of respiration and circulation, using mathematical models Several barriers exist to the development of ECPs: ... awareness, lack of familiarity with the protocol, lack of agreement, lack of demonstrated safety and efficacy, lack of known improved outcome, lack of ability to overcome the inertia of previous...

Ngày tải lên: 18/06/2014, 18:20

... field of adoptive cellular therapy of cancer The growing clinical applications and success of adoptive cellular therapy of cancer has been due to the rapid evolution of immunology, gene therapy ... cytotoxic T cells for melanoma therapy [8,9] Immune therapy of cancer has spread well beyond the treatment of melanoma The field of hematopoietic stem cell transplantation (HSCT) is moving from a cell ... list of author information is available at the end of the article and, in fact, are critical to the advancement of adoptive cellular therapy of cancer, but this and most other areas of clinical therapy...

Ngày tải lên: 20/06/2014, 04:20

... only intracellularly, prolonged expression of surface CTLA-4 could be a good marker for identifying Treg cells viable for the autologous cell therapy of chronic inflammations Polymorphisms of CTLA-4 ... CTLA-4-expressing cells represent a distinct pool of memory T cells [11,15] Control of apoptosis by CD28 and CTLA-4 The decision between the survival and apoptosis of T cells is of particular importance for ... prerequisite for cell cycling In addition, the induction of IL-2 leads to autocrine support for the activation of the cell cycle machinery; the IL-2 signal induces the degradation of the cell cycle...

Ngày tải lên: 09/08/2014, 01:23

... progenitors, and of the rate at which new cells are produced and progress from one developmental stage to another In adult mice, development of B cells occurs in the BM in a series of steps that ... and transitional B cells not decline with age, maintaining that ‘the major defect in B cell development of old mice is the inability of newly made cells to join the peripheral B cell compartment.’ ... gradual loss of naïve follicular B cells, but not of MZ or B1 B cells Recent studies conducted in our laboratory also suggest that reduced influx of B cells from the BM drives the selection of antigen-experienced...

Ngày tải lên: 09/08/2014, 01:23

... physical separation of T cells from monocytes through the use of a transwell insert abrogated this effect This observation was also true for Ttcr cells and RA synovial T cells but not for control nonactivated ... macrophages with either anti-CD3 for 48 hours (Ttcr cells) or a 'cocktail' of inflammatory cytokines (tumour necrosis factor alpha (TNFα), IL-2, IL-6) (Tck cells) for days, before fixation The unstimulated, ... differential utilization of NFκB for the expression of CC and CXC chemokines in this system We also examined the role of NFκB in the Tck -cell contactdependent production of chemokines in monocytes...

Ngày tải lên: 09/08/2014, 08:23

... deprived of nutrient factors for the following parameters: (a) the harvested cell number, (b) the proportion of Ki-67-immunopositive cells, (c) cell viability, and (d) the proportion of SA-βgal-positive ... alginate cultures resulted in the formation of NP cell clusters, which also form through increased cell proliferation in the NP in vivo and have been suggested to form part of an ineffective response ... and the proportion of SA-β-gal-positive cells was quantitated by scoring a minimum of 200 cells for each condition and each sample Collagen immunolocalisation The production of type I and II collagens...

Ngày tải lên: 09/08/2014, 10:23