il 10 transforming growth factor tgf β vegf sdf 1α and basic fibroblast growth factor bfgf in infarct related conditioned medium

Báo cáo y học: "Gender-based reciprocal expression of transforming growth factor-β1 and the inducible nitric oxide synthase in a rat model of cyclophosphamide-induced cystitis" ppsx

Ngày tải lên : 11/08/2014, 08:22

... interleukin (IL) -1alpha, IL- 1beta and soluble interleukin-1 receptor antagonist (sIL-1RA) but similar IL- 2, sIL2R, IL- 3, IL- 4, IL- 6, sIL-6R, IL- 10, tumour necrosis factor (TNF)-alpha, transforming growth ... β1 [pg/mg of Creatinine] 500 100 0 1500 2000 2500 Latent TGF β1 [pg/mg of Creatinine] Figure Relationship between urine TGF- β1 and NO2-/NO3- levels Inverse Inverse Relationship between urine TGF- β1 ... active and latent/total TGF- β1 at baseline and after CYP Active and latent/total TGF- β1 values are reported as pg/mg of creatinine Panel A Urine levels of TGF- β1 at baseline In the absence of CYP injection,...

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báo cáo khoa học: " The impact of hyperbaric oxygen therapy on serological values of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF)" pptx

Ngày tải lên : 11/08/2014, 20:20

... endothelial growth factor (VEGF) and basic growth factor (bFGF) In angiogenesis, a number of growth factors (for example Angiopoietin-1, Platelet derived growth factor, Hypoxia inducing factor- 1a, EphrinB2, ... endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) Head & Face Medicine 2 010 6:29 Submit your next manuscript to BioMed Central and take full advantage of: • Convenient online ... tyrosine kinase fibroblasts day 10 2.12 1.23 Characteristics of bFGF and VEGF 224 Serological values of bFGF in the study and the control group at definite times Jung et al Head & Face Medicine...

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Báo cáo y học: " Glucocorticoids activate TGF-β induced PAI-1 and CTGF expression in rat hepatocytes" potx

Ngày tải lên : 13/08/2014, 13:20

... JNK inhibitor II and Erk inhibitor on TGF- β and dexamethasone induced CTGF expression in hepatocytes Figure Effects of JNK inhibitor II and Erk inhibitor on TGF- β and dexamethasone induced CTGF ... analysed mRNA and protein expression of PAI-1 and CTGF by a combined stimulation with dexamethasone and TGF- β in primary rat hepatocytes and found that both proteins are co-induced by TGF and dexamethasone ... significantly enhanced TGF- βinduced PAI-1 in hepatocytes Similar to our findings in hepatocytes, Ma and coworkers found an increase of TGF -induced PAI-1 expression through dexamethasone in HTR-8 SV neo...

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Báo cáo y học: "Bone Morphogenetic Protein (BMP)-4 and BMP-7 regulate differentially Transforming Growth Factor (TGF)-β1 in normal human lung fibroblasts (NHLF)" pdf

Ngày tải lên : 12/08/2014, 11:22

... atmosphere in fibroblast growth medium (FGM, Lonza Rockland Inc, Rockland, ME, USA) supplemented with 0.5 ml recombinant human fibroblast growth factor- B, 0.5 ml insulin, 0.5 ml gentamicin sulphate ... BMP-4 and BMP-7 (Figure 4A) BMP-4 inhibited the TGF- β1 induced increase in the level of the transcripts encoding collagen type I and IV (Figures 3A and 3B), tenascin and fibronectin (Figures 4A and ... transfected in NHLF and TGF- β responsive Smad-binding elements (SBE) reporter gene in the MFB-F11 cell line TGF- β1 increased luciferase activity in the pCT-sp 6-fold, indicative of CTGF promoter...

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Báo cáo khoa học: Control of transforming growth factor b signal transduction by small GTPases pot

Ngày tải lên : 07/03/2014, 01:20

... small GTPases 96 97 98 99 100 101 102 103 104 105 106 107 108 109 induces nuclear import of Smad3 in an importin-b1 and Ran-dependent manner Mol Biol Cell 12, 107 9 109 1 Xiao Z, Latek R & Lodish ... [128–132] Eventually, LIM-kinases and phosphorylate actin-depolymerizing proteins such as coﬁlin, destrin and actin-depolymerizing factor, which are inactivated and thus permit actin polymerization to ... attenuated TGFb TGFb signaling and small GTPases signaling by increasing the segregation of TGFb receptors into a lipid raft–caveolar compartment [48], whereas ADAM12 (a disintegrin and metalloproteinase)...

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Báo cáo khoa học: Fragile X-related protein FXR1 controls posttranscriptional suppression of lipopolysaccharide-induced tumour necrosis factor-a production by transforming growth factor-b1 doc

Ngày tải lên : 22/03/2014, 21:21

... the cytokines involved in the induction of FXR1 remain uncharacterized The regulation by the anti -in ammatory cytokine transforming growth factor- b1 (TGF- b1) of the proinﬂammatory cytokine TNF-a ... TNF-a [50], interleukin (IL) -3 [51], and IL- 8 [52] Inhibiting p38 FEBS Journal 277 (2 010) 2754–2765 ª 2 010 The Authors Journal compilation ª 2 010 FEBS T K Khera et al TGF inhibits LPS induction ... with 10% fetal bovine serum, 10 UÆmL)1 penicillin, 10 lgÆmL)1 streptomycin, and mm l-glutamine (all from Invitrogen, Paisley, UK) Cells were maintained at 37 °C in the presence of 5% CO2 Inhibitors...

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Báo cáo khoa học: The C-terminus of viral vascular endothelial growth factor-E partially blocks binding to VEGF receptor-1 docx

Ngày tải lên : 23/03/2014, 07:20

... viral VEGF s C-terminus in VEGFR binding Fig Deletion of the C-terminus of ORFVNZ 2VEGF increases binding to VEGFR-1 and VEGFR-2 Immobilized VEGFR-1–Ig (A) or VEGFR-2– Ig (B) fusion protein was incubated ... viral VEGF s C-terminus in VEGFR binding M K Inder et al VEGF- A [10, 22], and that viral VEGFs conserve many residues shown to be vital for VEGF- A binding to VEGFR-1 [23,24] Although domain-exchange ... binding and cross-linking of VEGFR-1 and VEGFR-2, using BaF3-derived cell lines expressing chimeric receptors consisting of the extracellular, ligand-binding domains of human VEGFR-1 or mouse VEGFR-2...

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Báo cáo y học: Identiﬁcation of an element within the promoter of human selenoprotein P responsive to transforming growth factor-b ppt

Ngày tải lên : 31/03/2014, 23:20

... Cell culture and treatment The human hepatocarcinoma line HepG2 was cultured in RPMI 1640 medium containing 10% fetal bovine serum, 1% penicillin/streptomycin, mg:mL21 L -glutamine, and 0.15% NaHCO3 ... Identiﬁcation and functional characterization of a Smad binding element (SBE) in the JunB promoter that acts as a transforming growth factor- beta, activin, and bone morphogenetic proteininducible ... Cells were maintained under standard conditions at 37 8C in 5% CO2 Forty-eight hours before treatment, cells were cultured in RPMI 1640 medium containing 5% BMS, 1% penicillin/streptomycin, mg:mL21...

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transforming growth factor-beta protocols

Ngày tải lên : 11/04/2014, 07:14

... Measuring TGF- Growth 1 In Vitro Assays for Measuring TGFGrowth Stimulation and Inhibition Maryanne Edens and Edward B Leof Introduction Transforming growth factors (TGFs) were initially ... of TGFRadioiodinated transforming growth factors (TGF- s) are critical reagents for functional studies on TGF- ligand–receptor binding (1–5), TGF- –binding protein interactions (6–9), ligand ... thawing Bovine serum albumin (BSA), store at 4°C Penicillin–streptomycin–L-glutamine (PSG) stock (100 ×): 20 g/L strepto mycin, 50 × 106 U/L penicillin G, 29.2 g/L L-glutamine Filter sterilize and...

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báo cáo hóa học:" Enhanced serum concentrations of transforming growth factor-beta1 in simple fatty liver: is it really benign?" pot

Ngày tải lên : 18/06/2014, 15:20

... 0.055 ALT TGF- β1 r = -0.02 P = 0.9 TGF- β1 r = -0.13 P = 0.38 TGF- β1 r = -0.34 P = 0.026 BMI TGF- β1 rho = 0.13 P = 0.46 TGF- β1 rho = 0.18 P = 0.22 TGF- β1 rho = 0.09 P = 0.56 US score ND TGF- β1 rho ... serum TGF- β1 and ferritin TGF- β1 , transforming growth factor- beta1 To let us get down to the grassroots of the FL benignity from the beginning we should relay on longitudinal observations lasting ... = -0.08 P = 0.57 TGF- β1 rho = 0.05 P = 0.72 Fibrosis score TGF- β1 rho = -0.09 P = 0.60 ND TGF- β1 rho = 0.05 P = 0.75 Age TGF- β1 r = -0.02 P = 0.9 TGF- β1 r = 0.02 P = 0.9 TGF- β1 r = -0.009 P =...

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báo cáo hóa học: " Effect of anti-inflammatory agents on transforming growth factor beta over-expressing mouse brains: a model revised" potx

Ngày tải lên : 19/06/2014, 22:20

... reporting up-regulation of APP mRNA and protein, and increased production of Aβ40 and Aβ42 in 6month-old TGF- β1 heterozygous mice [38] This and one other study [39] also showed that TGF- β1 induces ... are 100 µM mouse Aβ and hence should detect endogenous mouse Aβ in TGF- β1 mice ELISA measurements of Aβ40 and Aβ42 at and months revealed no significant differences between control and TGF- β1 ... occur in the mouse brain [37] An increase in Aβ40 was however detected in TGF- β1 mice months old, suggesting changes in APP metabolism later in the life of the animals This finding reproduces in...

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Báo cáo y học: "Adverse effects of adenovirus-mediated gene transfer of human transforming growth factor beta 1 into rabbit knees" potx

Ngày tải lên : 09/08/2014, 01:21

... transforming growth factor (TGF) -β1 gene expression in naïve and antigen-induced arthritis (AIA) rabbit joints (A) × 107 , × 108 and × 109 adenoviral particles encoding human TGF- β1 cDNA were injected ... following intraarticular injection of the low dose (1 × 107 particles) of Ad .TGF- β1 Alterations in joint anatomy after intra-articular β Ad .TGF- β1 injection Three days after injection of Ad .TGF- β1 , ... Intra-articular injection of adenoviral vector expressing human transforming growth factor (Ad .TGF) -β1 resulted in a high level of TGF- β1 accumulation in the synovial fluid Intra-articular TGF- β1 expression...

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Báo cáo y học: "β Transforming growth factor-β-induced regulatory T cells referee inflammatory and autoimmune diseases" potx

Ngày tải lên : 09/08/2014, 06:22

... changes in aberrant immunity In mediating its suppressive effects, TGF- β signals through the type I and type II TGF- β serine–threonine kinase receptors, TβRI and TβRII Interaction of the TGF- β ligand ... Microbes Infect 1999, 1:1255-1263 31 Oklu R, Hesketh R: The latent transforming growth factor β binding protein (LTBP) family Biochem J 2000, 352:601- 610 β 32 Shi Y, Massagué J: Mechanisms of TGF- β ... [6,8,12] β TGF- β signaling and regulation TGF- β is a potent cytokine and growth factor whose biological activity is primarily regulated post-translationally [30], because it is transcribed and translated...

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Báo cáo y học: "Reduced transforming growth factor-beta signaling in cartilage of old mice: role in impaired repair capacity" ppsx

Ngày tải lên : 09/08/2014, 07:20

... various transforming growth factor (TGF) -beta signaling Staining ofin cartilage molecules in cartilage Paraffin sections of knee joints of young and old mice were stained with antibodies against ... cartilage cartilage repair capacity Effect of transforming growth factor (TGF) -beta deprivation on intrinsic cartilage repair capacity Murine knee joints of young mice were injected with IL- 1 ... articular cartilage Injection of IL- 1 24 h prior to knee joint isolation resulted in an increased expression of TGF- beta1 and TGF- beta2 in lateral tibial cartilage in old mice and a higher number...

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Báo cáo y học: " Aging, osteoarthritis and transforming growth factor-β signaling in cartilage" pps

Ngày tải lên : 09/08/2014, 07:20

... treatment with IL- 1 This indicates that the TGF- β signaling induced as a repair response to IL- 1 is curtailed by TGF- β depleting LAP The LAP binds to TGF- β and prevents its bioavailability to the ... R-Smads and the C-Smad have a highly conserved Mad homology domains (MH in the amino terminus and MH in the carboxy terminus) References Binding of the TGF- β isoforms to the TGF- β receptors (TGF- βRs) ... localized in the joints of young and old mice In 2-yearold mice there was a decline in the expression of TGF- β2 , TGF- β3 and TGF- β receptor It is noteworthy that the number of cells expressing phosphorylated...

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Báo cáo y học: "Activation of transforming growth factor-β1 and early atherosclerosis in systemic lupus erythematosus" pptx

Ngày tải lên : 09/08/2014, 08:22

... erythematosus; TGF, transforming growth factor A relative Activation Index (AI) was calculated, by division of TGF- β1 levels in blood (A450) incubated at 37°C for 90 minutes by TGF- β1 levels in blood ... latent TGF- β1 in an in vitro assay that utilizes the physiological activation of latent TGF- β1 that occurs normally during blood clotting The activation of TGF- β1 during clotting is complex, being ... anticoagulant TGF- β1 activation index: an in vitro measure of the in vivo efficiency of TGF- β1 activation To examine the ability of SLE patients and control individuals to activate TGF- β1 , a ratio...

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Báo cáo y học: "Constitutive upregulation of the transforming growth factor-β pathway in rheumatoid arthritis synovial fibroblasts" doc

Ngày tải lên : 09/08/2014, 10:20

... mRNA-expression of the transforming growth factor (TGF) -β related genes (a) TGF- β1 , (b) TGF- β2 , (c) TGF- β3 , (d) TGF- β receptor (TGFBR1), genes: (e) thrombospondin (THBS1), and (f) skiL in osteoarthritis ... molecules within the pathway in conjunction with their scores is demonstrated in Figure The mRNA for TGF- β1 and TGF- β3 , TGFBR1, the latent TGF binding proteins 1/2 (LTBP1/2), the TGF- β- releasing THBS1, ... TGF- β1 in RA (see above), resulting in more TGF- β1 activity in the arthritic joint Indeed, increased levels of active TGF- β1 have recently been reported in RA synovial fluid in Available online...

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Báo cáo y học: "Microarray gene expression profiling of osteoarthritic bone suggests altered bone remodelling, WNT and transforming growth factor-β/bone morphogenic protein signalling" pot

Ngày tải lên : 09/08/2014, 10:21

... also interacts with and influences the effects of key bone regulators insulin-like growth factor (IGF)1 and transforming growth factor (TGF) -β [93,94] STC1 Stanniocalcin inhibits calcium uptake and ... identified in OA individuals altered levels of insulin-like growth factor- 1, insulin-like growth factor- 2 and TGF- β1 in cortical bone from the iliac crest [38]; matrix metalloproteinase (MMP)2 and liver ... TGFB1 and BMP5, a member of the transforming growth factor (TGF) -β superfamily, as OA susceptibility loci has implicated the TGF- β/ BMP signalling pathway in OA pathogenesis The TGF- β/ BMP signalling...

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Báo cáo y học: "Inorganic pyrophosphate generation by transforming growth factor-beta-1 is mainly dependent on ANK induction by Ras/Raf-1/extracellular signal-regulated kinase pathways in chondrocytes" docx

Ngày tải lên : 09/08/2014, 10:22

... of 10 ng/mL of TGF- β1 (prepared from a stock solution at 10 μg/mL in mM citric acid containing mg/mL bovine serum albumin) in DMEM/F-12 containing a final concentration of 1% FCS Third, to investigate ... murine cartilage and bone [18] TGF- β1 was shown to induce two major signaling pathways, referred to as TGF- β1 Smad-dependent [19] or Smadindependent [20] signaling, in many cell types However, intracellular ... several signaling pathways in transforming growth factor- beta-1 (TGF- β1 )-induced expression of the Ank gene (a) Kinetics of signgene aling events induced by TGF- β1 Total proteins were extracted from...

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Báo cáo y học: "Adipose-derived mesenchymal stem cells from the sand rat: transforming growth factor beta and 3D co-culture with human disc cells stimulate proteoglycan and collagen type I rich extracellular matrix" ppsx

Ngày tải lên : 09/08/2014, 10:23

... inducers of chondrogenic differentiation are members of the transforming growth factor- beta (TGF ) super family such as the TGF isoforms and the BMPs [15] Also important are the fibroblast growth ... fibroblast growth factor isoforms and insulinlike growth factor (IGF-1) TGF super family cytokines act through binding to cell-surface receptors Differentiation occurs through two major intracellular ... immunohistochemistry and toluidine blue staining Scoring of slides from immunohistochemical staining of cellsurface markers, ECM proteins and toluidine blue staining of total proteoglycans was performed blinded...

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